Restriction map for
pF1KA0209
(Linear) (Six-base) MAPSORT of: KIAA0209.seq Check: 5515 from: 1 to: 5490
KIAA0209.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2364 2479 5490
Size: 2364 115 3011
Fragments arranged by size:
3011 2364 115
AccI GT'mk_AC
Cuts at: 0 4358 5490
Size: 4358 1132
Acc65I G'GTAC_C
Cuts at: 0 3665 5490
Size: 3665 1825
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 451 847 1882 2170 2230 2614 3421
Size: 442 9 396 1035 288 60 384 807
Cuts at: 3421 4676 5179 5468 5490
Size: 1255 503 289 22
Fragments arranged by size:
1255 1035 807 503 442 396 384 289
288 60 22 9
AflII C'TTAA_G
Cuts at: 0 919 5490
Size: 919 4571
AflIII A'CryG_T
Cuts at: 0 118 2948 4457 5490
Size: 118 2830 1509 1033
Fragments arranged by size:
2830 1509 1033 118
AhdI GACnn_n'nnGTC
Cuts at: 0 5475 5490
Size: 5475 15
AleI CACnn'nnGTG
Cuts at: 0 1216 1813 5490
Size: 1216 597 3677
Fragments arranged by size:
3677 1216 597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 957 1393 2129 5490
Size: 957 436 736 3361
Fragments arranged by size:
3361 957 736 436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2212 2244 5490
Size: 2212 32 3246
Fragments arranged by size:
3246 2212 32
AlwNI CAG_nnn'CTG
Cuts at: 0 954 2637 2745 3456 3718 3963 4279 5490
Size: 954 1683 108 711 262 245 316 1211
Fragments arranged by size:
1683 1211 954 711 316 262 245 108
ApaLI G'TGCA_C
Cuts at: 0 942 4453 5490
Size: 942 3511 1037
Fragments arranged by size:
3511 1037 942
ApoI r'AATT_y
Cuts at: 0 251 875 1896 2062 3624 3979 4953 5490
Size: 251 624 1021 166 1562 355 974 537
Fragments arranged by size:
1562 1021 974 624 537 355 251 166
AvaI C'yCGr_G
Cuts at: 0 1787 4179 5055 5490
Size: 1787 2392 876 435
Fragments arranged by size:
2392 1787 876 435
BamHI G'GATC_C
Cuts at: 0 1511 4430 5490
Size: 1511 2919 1060
Fragments arranged by size:
2919 1511 1060
BanI G'GyrC_C
Cuts at: 0 3665 5490
Size: 3665 1825
BanII G_rGCy'C
Cuts at: 0 72 719 1756 3308 5490
Size: 72 647 1037 1552 2182
Fragments arranged by size:
2182 1552 1037 647 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 509 828 1505 2158 2988 5392 5490
Size: 509 319 677 653 830 2404 98
Fragments arranged by size:
2404 830 677 653 509 319 98
BbvCI CC'TCA_GC
Cuts at: 0 5347 5490
Size: 5347 143
BciVI GTATCCnnnnn_n'
Cuts at: 0 133 918 1944 2157 4030 5490
Size: 133 785 1026 213 1873 1460
Fragments arranged by size:
1873 1460 1026 785 213 133
BglI GCCn_nnn'nGGC
Cuts at: 0 1091 5490
Size: 1091 4399
BglII A'GATC_T
Cuts at: 0 3932 5490
Size: 3932 1558
BlpI GC'TnA_GC
Cuts at: 0 713 1744 3302 3952 5490
Size: 713 1031 1558 650 1538
Fragments arranged by size:
1558 1538 1031 713 650
Bme1580I G_kGCm'C
Cuts at: 0 946 4457 5490
Size: 946 3511 1033
Fragments arranged by size:
3511 1033 946
BmgBI CAC'GTC
Cuts at: 0 119 446 5490
Size: 119 327 5044
Fragments arranged by size:
5044 327 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 4134 5490
Size: 4134 1356
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1446 3156 4416 5490
Size: 1446 1710 1260 1074
Fragments arranged by size:
1710 1446 1260 1074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1740 1930 2107 2154 2476 2626 2659
Size: 328 1412 190 177 47 322 150 33
Cuts at: 2659 2978 3151 3478 3565 4504 5490
Size: 319 173 327 87 939 986
Fragments arranged by size:
1412 986 939 328 327 322 319 190
177 173 150 87 47 33
Bpu10I CC'TnA_GC
Cuts at: 0 2686 5347 5490
Size: 2686 2661 143
Fragments arranged by size:
2686 2661 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1639 3736 3811 4690 5212 5490
Size: 496 1143 2097 75 879 522 278
Fragments arranged by size:
2097 1143 879 522 496 278 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 2863 2905 3838 4552 5490
Size: 2863 42 933 714 938
Fragments arranged by size:
2863 938 933 714 42
BsaAI yAC'GTr
Cuts at: 0 4314 5490
Size: 4314 1176
BsaBI GATnn'nnATC
Cuts at: 0 3931 4250 4753 4930 5490
Size: 3931 319 503 177 560
Fragments arranged by size:
3931 560 503 319 177
BsaHI Gr'CG_yC
Cuts at: 0 5023 5233 5490
Size: 5023 210 257
Fragments arranged by size:
5023 257 210
BsaWI w'CCGG_w
Cuts at: 0 2057 2759 3308 4799 5490
Size: 2057 702 549 1491 691
Fragments arranged by size:
2057 1491 702 691 549
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 1916 1946 2632 2662 3640 3670
Size: 367 30 1519 30 686 30 978 30
Cuts at: 3670 4175 4205 4564 4594 4831 4861 5490
Size: 505 30 359 30 237 30 629
Fragments arranged by size:
1519 978 686 629 505 367 359 237
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 636 972 3664 5490
Size: 636 336 2692 1826
Fragments arranged by size:
2692 1826 636 336
BseYI C'CCAG_C
Cuts at: 0 74 386 1089 1230 1851 4744 4972 5283
Size: 74 312 703 141 621 2893 228 311
Cuts at: 5283 5490
Size: 207
Fragments arranged by size:
2893 703 621 312 311 228 207 141
74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 714 3591 5490
Size: 370 344 2877 1899
Fragments arranged by size:
2877 1899 370 344
BsiEI CG_ry'CG
Cuts at: 0 2248 5490
Size: 2248 3242
BsiHKAI G_wGCw'C
Cuts at: 0 719 946 1756 1822 3308 3491 4457 5065
Size: 719 227 810 66 1486 183 966 608
Cuts at: 5065 5490
Size: 425
Fragments arranged by size:
1486 966 810 719 608 425 227 183
66
BsmI GAATG_Cn'
Cuts at: 0 1986 2560 4203 4494 5490
Size: 1986 574 1643 291 996
Fragments arranged by size:
1986 1643 996 574 291
BsmBI CGTCTCn'nnnn_
Cuts at: 0 109 2304 3180 3826 5490
Size: 109 2195 876 646 1664
Fragments arranged by size:
2195 1664 876 646 109
Bsp1286I G_dGCh'C
Cuts at: 0 72 719 946 1756 1822 3308 3491 4457
Size: 72 647 227 810 66 1486 183 966
Cuts at: 4457 5065 5490
Size: 608 425
Fragments arranged by size:
1486 966 810 647 608 425 227 183
72 66
BspEI T'CCGG_A
Cuts at: 0 3308 5490
Size: 3308 2182
BspHI T'CATG_A
Cuts at: 0 1976 3995 5186 5490
Size: 1976 2019 1191 304
Fragments arranged by size:
2019 1976 1191 304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2364 2479 3626 5490
Size: 2364 115 1147 1864
Fragments arranged by size:
2364 1864 1147 115
BsrBI CCG'CTC
Cuts at: 0 30 5490
Size: 30 5460
BsrDI GCAATG_nn'
Cuts at: 0 635 2048 2191 3497 5490
Size: 635 1413 143 1306 1993
Fragments arranged by size:
1993 1413 1306 635 143
BsrFI r'CCGG_y
Cuts at: 0 3818 5490
Size: 3818 1672
BsrGI T'GTAC_A
Cuts at: 0 3656 3671 5490
Size: 3656 15 1819
Fragments arranged by size:
3656 1819 15
BssSI C'ACGA_G
Cuts at: 0 3412 5490
Size: 3412 2078
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3447 3605 5490
Size: 3447 158 1885
Fragments arranged by size:
3447 1885 158
BstEII G'GTnAC_C
Cuts at: 0 1140 2842 5490
Size: 1140 1702 2648
Fragments arranged by size:
2648 1702 1140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1218 1230 2979 3510 5490
Size: 1218 12 1749 531 1980
Fragments arranged by size:
1980 1749 1218 531 12
BstYI r'GATC_y
Cuts at: 0 641 843 915 1511 3932 4430 4523 4727
Size: 641 202 72 596 2421 498 93 204
Cuts at: 4727 4754 5462 5490
Size: 27 708 28
Fragments arranged by size:
2421 708 641 596 498 204 202 93
72 28 27
Bsu36I CC'TnA_GG
Cuts at: 0 800 3296 4006 4679 5072 5490
Size: 800 2496 710 673 393 418
Fragments arranged by size:
2496 800 710 673 418 393
BtgI C'CryG_G
Cuts at: 0 1214 2774 4089 4508 5480 5490
Size: 1214 1560 1315 419 972 10
Fragments arranged by size:
1560 1315 1214 972 419 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 110 792 5490
Size: 110 682 4698
Fragments arranged by size:
4698 682 110
BtsI GCAGTG_nn'
Cuts at: 0 1767 3104 3775 5490
Size: 1767 1337 671 1715
Fragments arranged by size:
1767 1715 1337 671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1376 1411 5490
Size: 1376 35 4079
Fragments arranged by size:
4079 1376 35
DraI TTT'AAA
Cuts at: 0 2544 5490
Size: 2544 2946
DraIII CAC_nnn'GTG
Cuts at: 0 39 1200 1722 2808 5490
Size: 39 1161 522 1086 2682
Fragments arranged by size:
2682 1161 1086 522 39
DrdI GACnn_nn'nnGTC
Cuts at: 0 1648 2014 4612 5490
Size: 1648 366 2598 878
Fragments arranged by size:
2598 1648 878 366
EaeI y'GGCC_r
Cuts at: 0 41 320 1220 2969 4044 4511 5216 5432
Size: 41 279 900 1749 1075 467 705 216
Cuts at: 5432 5490
Size: 58
Fragments arranged by size:
1749 1075 900 705 467 279 216 58
41
EarI CTCTTCn'nnn_
Cuts at: 0 724 1972 3878 4104 4652 5092 5490
Size: 724 1248 1906 226 548 440 398
Fragments arranged by size:
1906 1248 724 548 440 398 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3928 5490
Size: 3928 1562
EcoICRI GAG'CTC
Cuts at: 0 717 1754 3306 5490
Size: 717 1037 1552 2184
Fragments arranged by size:
2184 1552 1037 717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 442 451 847 1740 1882 1930 2107
Size: 328 114 9 396 893 142 48 177
Cuts at: 2107 2154 2170 2230 2476 2614 2626 2659 2978
Size: 47 16 60 246 138 12 33 319
Cuts at: 2978 3151 3421 3478 3565 4504 4676 5179 5468
Size: 173 270 57 87 939 172 503 289
Cuts at: 5468 5490
Size: 22
Fragments arranged by size:
939 893 503 396 328 319 289 270
246 177 173 172 142 138 114 87
60 57 48 47 33 22 16 12
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2371 5071 5490
Size: 2371 2700 419
Fragments arranged by size:
2700 2371 419
EcoO109I rG'GnC_Cy
Cuts at: 0 383 962 2634 2931 3268 4710 5490
Size: 383 579 1672 297 337 1442 780
Fragments arranged by size:
1672 1442 780 579 383 337 297
EcoRI G'AATT_C
Cuts at: 0 4953 5490
Size: 4953 537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 852 1053 2175 4665 5490
Size: 852 201 1122 2490 825
Fragments arranged by size:
2490 1122 852 825 201
FspI TGC'GCA
Cuts at: 0 4345 5490
Size: 4345 1145
HaeII r_GCGC'y
Cuts at: 0 5296 5490
Size: 5296 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 367 383 399 1500 1532 1783 1815
Size: 351 16 16 16 1101 32 251 32
Cuts at: 1815 2331 2363 5461 5490
Size: 516 32 3098 29
Fragments arranged by size:
3098 1101 516 351 251 32 32 32
29 16 16 16
HindIII A'AGCT_T
Cuts at: 0 3226 5490
Size: 3226 2264
KpnI G_GTAC'C
Cuts at: 0 3669 5490
Size: 3669 1821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2124 3740 4014 4331 5132 5300 5388 5490
Size: 2124 1616 274 317 801 168 88 102
Fragments arranged by size:
2124 1616 801 317 274 168 102 88
MscI TGG'CCA
Cuts at: 0 43 322 1222 2971 5434 5490
Size: 43 279 900 1749 2463 56
Fragments arranged by size:
2463 1749 900 279 56 43
MslI CAynn'nnrTG
Cuts at: 0 557 1150 1216 1351 1813 1978 2111 2773
Size: 557 593 66 135 462 165 133 662
Cuts at: 2773 2977 5490
Size: 204 2513
Fragments arranged by size:
2513 662 593 557 462 204 165 135
133 66
MspA1I CmG'CkG
Cuts at: 0 65 1063 2742 3072 3129 3453 4091 4131
Size: 65 998 1679 330 57 324 638 40
Cuts at: 4131 5143 5490
Size: 1012 347
Fragments arranged by size:
1679 1012 998 638 347 330 324 65
57 40
NcoI C'CATG_G
Cuts at: 0 2774 5490
Size: 2774 2716
NspI r_CATG'y
Cuts at: 0 1506 2625 2816 3450 4461 5215 5490
Size: 1506 1119 191 634 1011 754 275
Fragments arranged by size:
1506 1119 1011 754 634 275 191
PasI CC'CwG_GG
Cuts at: 0 5314 5490
Size: 5314 176
PciI A'CATG_T
Cuts at: 0 4457 5490
Size: 4457 1033
PflMI CCAn_nnn'nTGG
Cuts at: 0 2637 5490
Size: 2637 2853
PfoI T'CCnGG_A
Cuts at: 0 637 1946 2636 3542 5170 5490
Size: 637 1309 690 906 1628 320
Fragments arranged by size:
1628 1309 906 690 637 320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4156 4188 5490
Size: 4156 32 1302
Fragments arranged by size:
4156 1302 32
PpuMI rG'GwC_Cy
Cuts at: 0 383 2634 2931 3268 4710 5490
Size: 383 2251 297 337 1442 780
Fragments arranged by size:
2251 1442 780 383 337 297
PshAI GACnn'nnGTC
Cuts at: 0 1539 4926 5490
Size: 1539 3387 564
Fragments arranged by size:
3387 1539 564
PsiI TTA'TAA
Cuts at: 0 4291 5490
Size: 4291 1199
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 5490
Size: 1261 32 4197
Fragments arranged by size:
4197 1261 32
PstI C_TGCA'G
Cuts at: 0 89 249 3128 3486 4966 5148 5490
Size: 89 160 2879 358 1480 182 342
Fragments arranged by size:
2879 1480 358 342 182 160 89
PvuII CAG'CTG
Cuts at: 0 1063 2742 3072 3129 3453 4131 5143 5490
Size: 1063 1679 330 57 324 678 1012 347
Fragments arranged by size:
1679 1063 1012 678 347 330 324 57
SacI G_AGCT'C
Cuts at: 0 719 1756 3308 5490
Size: 719 1037 1552 2182
Fragments arranged by size:
2182 1552 1037 719
SacII CC_GC'GG
Cuts at: 0 4092 5490
Size: 4092 1398
SapI GCTCTTCn'nnn_
Cuts at: 0 724 5490
Size: 724 4766
ScaI AGT'ACT
Cuts at: 0 2650 5490
Size: 2650 2840
SexAI A'CCwGG_T
Cuts at: 0 1196 2630 3527 5490
Size: 1196 1434 897 1963
Fragments arranged by size:
1963 1434 1196 897
SfcI C'TryA_G
Cuts at: 0 85 245 1888 3124 3482 4962 5144 5490
Size: 85 160 1643 1236 358 1480 182 346
Fragments arranged by size:
1643 1480 1236 358 346 182 160 85
SmaI CCC'GGG
Cuts at: 0 1789 4181 5057 5490
Size: 1789 2392 876 433
Fragments arranged by size:
2392 1789 876 433
SmlI C'TyrA_G
Cuts at: 0 475 919 1654 3751 3826 4705 5227 5490
Size: 475 444 735 2097 75 879 522 263
Fragments arranged by size:
2097 879 735 522 475 444 263 75
SphI G_CATG'C
Cuts at: 0 5215 5490
Size: 5215 275
StuI AGG'CCT
Cuts at: 0 1133 3539 4645 5490
Size: 1133 2406 1106 845
Fragments arranged by size:
2406 1133 1106 845
StyI C'CwwG_G
Cuts at: 0 57 410 1109 2120 2774 5490
Size: 57 353 699 1011 654 2716
Fragments arranged by size:
2716 1011 699 654 353 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 673 1128 1150 1529 2234 2830 3127 3506
Size: 673 455 22 379 705 596 297 379
Cuts at: 3506 5031 5490
Size: 1525 459
Fragments arranged by size:
1525 705 673 596 459 455 379 379
297 22
TatI w'GTAC_w
Cuts at: 0 1310 1439 2505 2648 3443 3656 3671 5490
Size: 1310 129 1066 143 795 213 15 1819
Fragments arranged by size:
1819 1310 1066 795 213 143 129 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1916 1948 2933 2965 3754 3786 4175 4207
Size: 1916 32 985 32 789 32 389 32
Cuts at: 4207 4461 4493 5490
Size: 254 32 997
Fragments arranged by size:
1916 997 985 789 389 254 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1152 1809 4938 5286 5371 5490
Size: 1152 657 3129 348 85 119
Fragments arranged by size:
3129 1152 657 348 119 85
XmaI C'CCGG_G
Cuts at: 0 1787 4179 5055 5490
Size: 1787 2392 876 435
Fragments arranged by size:
2392 1787 876 435
XmnI GAAnn'nnTTC
Cuts at: 0 1072 4645 5490
Size: 1072 3573 845
Fragments arranged by size:
3573 1072 845
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HindIII KpnI MmeI MscI
MslI MspA1I NcoI NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PsiI PsrI PstI PvuII SacI
SacII SapI ScaI SexAI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI ApaI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BclI BfrBI BmtI BsiWI
BssHII BstBI BstZ17I ClaI EagI EcoRV FseI FspAI
HincII HpaI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PspOMI PspXI PvuI RsrII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI ZraI