Restriction map for
pF1KSDA0212
(Linear) (Six-base) MAPSORT of: KIAA0212.seq Check: 7985 from: 1 to: 1971
KIAA0212.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1326 1971
Size: 1326 645
AccI GT'mk_AC
Cuts at: 0 951 1971
Size: 951 1020
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1820 1971
Size: 1820 151
AfeI AGC'GCT
Cuts at: 0 14 106 349 1971
Size: 14 92 243 1622
Fragments arranged by size:
1622 243 92 14
AflII C'TTAA_G
Cuts at: 0 1206 1971
Size: 1206 765
AflIII A'CryG_T
Cuts at: 0 1271 1724 1971
Size: 1271 453 247
Fragments arranged by size:
1271 453 247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 1954 1971
Size: 395 1559 17
Fragments arranged by size:
1559 395 17
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1341 1560 1971
Size: 135 1206 219 411
Fragments arranged by size:
1206 411 219 135
ApaI G_GGCC'C
Cuts at: 0 86 176 494 1971
Size: 86 90 318 1477
Fragments arranged by size:
1477 318 90 86
ApaLI G'TGCA_C
Cuts at: 0 1834 1971
Size: 1834 137
ApoI r'AATT_y
Cuts at: 0 591 934 1789 1971
Size: 591 343 855 182
Fragments arranged by size:
855 591 343 182
AvaI C'yCGr_G
Cuts at: 0 168 854 1971
Size: 168 686 1117
Fragments arranged by size:
1117 686 168
BanI G'GyrC_C
Cuts at: 0 358 1096 1408 1971
Size: 358 738 312 563
Fragments arranged by size:
738 563 358 312
BanII G_rGCy'C
Cuts at: 0 86 145 176 247 464 494 1971
Size: 86 59 31 71 217 30 1477
Fragments arranged by size:
1477 217 86 71 59 31 30
BbeI G_GCGC'C
Cuts at: 0 362 1971
Size: 362 1609
BbsI GAAGACnn'nnnn_
Cuts at: 0 63 1166 1634 1697 1971
Size: 63 1103 468 63 274
Fragments arranged by size:
1103 468 274 63 63
BciVI GTATCCnnnnn_n'
Cuts at: 0 207 564 1971
Size: 207 357 1407
Fragments arranged by size:
1407 357 207
BclI T'GATC_A
Cuts at: 0 626 1971
Size: 626 1345
BfrBI ATG'CAT
Cuts at: 0 565 1971
Size: 565 1406
BglI GCCn_nnn'nGGC
Cuts at: 0 149 210 1971
Size: 149 61 1761
Fragments arranged by size:
1761 149 61
BlpI GC'TnA_GC
Cuts at: 0 115 1971
Size: 115 1856
Bme1580I G_kGCm'C
Cuts at: 0 86 176 384 494 1413 1838 1971
Size: 86 90 208 110 919 425 133
Fragments arranged by size:
919 425 208 133 110 90 86
BmgBI CAC'GTC
Cuts at: 0 1608 1971
Size: 1608 363
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 1080 1971
Size: 306 774 891
Fragments arranged by size:
891 774 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1857 1971
Size: 1857 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 1238 1464 1651 1971
Size: 1238 226 187 320
Fragments arranged by size:
1238 320 226 187
BsaAI yAC'GTr
Cuts at: 0 306 1971
Size: 306 1665
BsaBI GATnn'nnATC
Cuts at: 0 1763 1971
Size: 1763 208
BsaHI Gr'CG_yC
Cuts at: 0 264 359 1971
Size: 264 95 1612
Fragments arranged by size:
1612 264 95
BsaWI w'CCGG_w
Cuts at: 0 1016 1631 1971
Size: 1016 615 340
Fragments arranged by size:
1016 615 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1861 1891 1971
Size: 1861 30 80
Fragments arranged by size:
1861 80 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 153 802 1489 1920 1971
Size: 25 128 649 687 431 51
Fragments arranged by size:
687 649 431 128 51 25
BseYI C'CCAG_C
Cuts at: 0 78 85 288 309 1971
Size: 78 7 203 21 1662
Fragments arranged by size:
1662 203 78 21 7
BsiEI CG_ry'CG
Cuts at: 0 318 486 498 1971
Size: 318 168 12 1473
Fragments arranged by size:
1473 318 168 12
BsiHKAI G_wGCw'C
Cuts at: 0 35 464 1838 1971
Size: 35 429 1374 133
Fragments arranged by size:
1374 429 133 35
Bsp1286I G_dGCh'C
Cuts at: 0 35 86 145 176 247 384 464 494
Size: 35 51 59 31 71 137 80 30
Cuts at: 494 1413 1838 1971
Size: 919 425 133
Fragments arranged by size:
919 425 137 133 80 71 59 51
35 31 30
BspEI T'CCGG_A
Cuts at: 0 1016 1971
Size: 1016 955
BspHI T'CATG_A
Cuts at: 0 1851 1971
Size: 1851 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1326 1971
Size: 1326 645
BsrBI CCG'CTC
Cuts at: 0 114 1971
Size: 114 1857
BsrDI GCAATG_nn'
Cuts at: 0 10 1029 1050 1236 1971
Size: 10 1019 21 186 735
Fragments arranged by size:
1019 735 186 21 10
BsrFI r'CCGG_y
Cuts at: 0 182 916 1971
Size: 182 734 1055
Fragments arranged by size:
1055 734 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 296 1075 1960 1971
Size: 296 779 885 11
Fragments arranged by size:
885 779 296 11
BtgI C'CryG_G
Cuts at: 0 51 176 259 324 480 486 498 1780
Size: 51 125 83 65 156 6 12 1282
Cuts at: 1780 1971
Size: 191
Fragments arranged by size:
1282 191 156 125 83 65 51 12
6
BtsI GCAGTG_nn'
Cuts at: 0 474 1001 1971
Size: 474 527 970
Fragments arranged by size:
970 527 474
EaeI y'GGCC_r
Cuts at: 0 315 483 983 1971
Size: 315 168 500 988
Fragments arranged by size:
988 500 315 168
EagI C'GGCC_G
Cuts at: 0 315 483 1971
Size: 315 168 1488
Fragments arranged by size:
1488 315 168
EarI CTCTTCn'nnn_
Cuts at: 0 1206 1462 1796 1971
Size: 1206 256 334 175
Fragments arranged by size:
1206 334 256 175
EcoICRI GAG'CTC
Cuts at: 0 462 1971
Size: 462 1509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1820 1857 1971
Size: 1820 37 114
Fragments arranged by size:
1820 114 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1504 1971
Size: 1504 467
EcoO109I rG'GnC_Cy
Cuts at: 0 82 172 504 687 1443 1971
Size: 82 90 332 183 756 528
Fragments arranged by size:
756 528 332 183 90 82
EcoRI G'AATT_C
Cuts at: 0 1789 1971
Size: 1789 182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 756 1539 1809 1971
Size: 756 783 270 162
Fragments arranged by size:
783 756 270 162
FseI GG_CCGG'CC
Cuts at: 0 186 1971
Size: 186 1785
HaeII r_GCGC'y
Cuts at: 0 16 108 351 362 1971
Size: 16 92 243 11 1609
Fragments arranged by size:
1609 243 92 16 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 834 866 933 965 1293 1325
Size: 2 32 800 32 67 32 328 32
Cuts at: 1325 1971
Size: 646
Fragments arranged by size:
800 646 328 67 32 32 32 32
2
HincII GTy'rAC
Cuts at: 0 952 1263 1971
Size: 952 311 708
Fragments arranged by size:
952 708 311
KasI G'GCGC_C
Cuts at: 0 358 1971
Size: 358 1613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1056 1904 1971
Size: 1056 848 67
Fragments arranged by size:
1056 848 67
MscI TGG'CCA
Cuts at: 0 985 1971
Size: 985 986
MslI CAynn'nnrTG
Cuts at: 0 975 1936 1971
Size: 975 961 35
Fragments arranged by size:
975 961 35
MspA1I CmG'CkG
Cuts at: 0 261 326 356 375 482 500 910 1287
Size: 261 65 30 19 107 18 410 377
Cuts at: 1287 1437 1971
Size: 150 534
Fragments arranged by size:
534 410 377 261 150 107 65 30
19 18
NaeI GCC'GGC
Cuts at: 0 184 1971
Size: 184 1787
NarI GG'CG_CC
Cuts at: 0 359 1971
Size: 359 1612
NcoI C'CATG_G
Cuts at: 0 51 1780 1971
Size: 51 1729 191
Fragments arranged by size:
1729 191 51
NdeI CA'TA_TG
Cuts at: 0 1393 1971
Size: 1393 578
NgoMIV G'CCGG_C
Cuts at: 0 182 1971
Size: 182 1789
NsiI A_TGCA'T
Cuts at: 0 567 1971
Size: 567 1404
NspI r_CATG'y
Cuts at: 0 411 905 1275 1728 1941 1971
Size: 411 494 370 453 213 30
Fragments arranged by size:
494 453 411 370 213 30
PciI A'CATG_T
Cuts at: 0 1271 1724 1971
Size: 1271 453 247
Fragments arranged by size:
1271 453 247
PflMI CCAn_nnn'nTGG
Cuts at: 0 798 1971
Size: 798 1173
PpuMI rG'GwC_Cy
Cuts at: 0 504 687 1971
Size: 504 183 1284
Fragments arranged by size:
1284 504 183
PspOMI G'GGCC_C
Cuts at: 0 82 172 490 1971
Size: 82 90 318 1481
Fragments arranged by size:
1481 318 90 82
PstI C_TGCA'G
Cuts at: 0 218 1316 1337 1442 1556 1971
Size: 218 1098 21 105 114 415
Fragments arranged by size:
1098 415 218 114 105 21
PvuII CAG'CTG
Cuts at: 0 375 1287 1437 1971
Size: 375 912 150 534
Fragments arranged by size:
912 534 375 150
SacI G_AGCT'C
Cuts at: 0 464 1971
Size: 464 1507
SacII CC_GC'GG
Cuts at: 0 262 327 483 501 1971
Size: 262 65 156 18 1470
Fragments arranged by size:
1470 262 156 65 18
SalI G'TCGA_C
Cuts at: 0 950 1971
Size: 950 1021
SanDI GG'GwC_CC
Cuts at: 0 504 1971
Size: 504 1467
SfcI C'TryA_G
Cuts at: 0 214 351 1312 1333 1438 1552 1971
Size: 214 137 961 21 105 114 419
Fragments arranged by size:
961 419 214 137 114 105 21
SfoI GGC'GCC
Cuts at: 0 360 1971
Size: 360 1611
SmlI C'TyrA_G
Cuts at: 0 1206 1971
Size: 1206 765
SspI AAT'ATT
Cuts at: 0 1669 1971
Size: 1669 302
StuI AGG'CCT
Cuts at: 0 1318 1971
Size: 1318 653
StyI C'CwwG_G
Cuts at: 0 51 1780 1818 1971
Size: 51 1729 38 153
Fragments arranged by size:
1729 153 51 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 969 1084 1971
Size: 969 115 887
Fragments arranged by size:
969 887 115
TatI w'GTAC_w
Cuts at: 0 531 1568 1701 1971
Size: 531 1037 133 270
Fragments arranged by size:
1037 531 270 133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1322 1354 1817 1849 1971
Size: 1322 32 463 32 122
Fragments arranged by size:
1322 463 122 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1273 1522 1789 1971
Size: 1273 249 267 182
Fragments arranged by size:
1273 267 249 182
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstXI BtgI BtsI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI
SfcI SfoI SmlI SspI StuI StyI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AleI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BglII
BmtI BplI Bpu10I BpuEI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI
EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI