Restriction map for
pF1KSDA0214
(Linear) (Six-base) MAPSORT of: KIAA0214.seq Check: 1580 from: 1 to: 2271
KIAA0214.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1275 2271
Size: 1275 996
AatII G_ACGT'C
Cuts at: 0 233 2114 2271
Size: 233 1881 157
Fragments arranged by size:
1881 233 157
AccI GT'mk_AC
Cuts at: 0 1291 2271
Size: 1291 980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 983 2164 2271
Size: 592 391 1181 107
Fragments arranged by size:
1181 592 391 107
AflII C'TTAA_G
Cuts at: 0 85 2271
Size: 85 2186
AflIII A'CryG_T
Cuts at: 0 176 1430 2237 2271
Size: 176 1254 807 34
Fragments arranged by size:
1254 807 176 34
AleI CACnn'nnGTG
Cuts at: 0 1557 2271
Size: 1557 714
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 536 2156 2271
Size: 536 1620 115
Fragments arranged by size:
1620 536 115
AlwNI CAG_nnn'CTG
Cuts at: 0 225 2073 2256 2271
Size: 225 1848 183 15
Fragments arranged by size:
1848 225 183 15
ApaI G_GGCC'C
Cuts at: 0 1675 2271
Size: 1675 596
ApoI r'AATT_y
Cuts at: 0 1017 1204 2271
Size: 1017 187 1067
Fragments arranged by size:
1067 1017 187
AvaI C'yCGr_G
Cuts at: 0 799 1148 2117 2271
Size: 799 349 969 154
Fragments arranged by size:
969 799 349 154
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2236 2269 2271
Size: 2236 33 2
Fragments arranged by size:
2236 33 2
BanI G'GyrC_C
Cuts at: 0 1316 2271
Size: 1316 955
BanII G_rGCy'C
Cuts at: 0 451 800 1229 1675 1777 1919 2234 2271
Size: 451 349 429 446 102 142 315 37
Fragments arranged by size:
451 446 429 349 315 142 102 37
BbeI G_GCGC'C
Cuts at: 0 1320 2271
Size: 1320 951
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 1069 1622 2271
Size: 179 890 553 649
Fragments arranged by size:
890 649 553 179
BbvCI CC'TCA_GC
Cuts at: 0 65 2271
Size: 65 2206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 12 46 2271
Size: 12 34 2225
Fragments arranged by size:
2225 34 12
BclI T'GATC_A
Cuts at: 0 335 2271
Size: 335 1936
BfrBI ATG'CAT
Cuts at: 0 76 784 2271
Size: 76 708 1487
Fragments arranged by size:
1487 708 76
Bme1580I G_kGCm'C
Cuts at: 0 415 565 1675 2271
Size: 415 150 1110 596
Fragments arranged by size:
1110 596 415 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 1044 1429 2271
Size: 1044 385 842
Fragments arranged by size:
1044 842 385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 759 1062 2271
Size: 759 303 1209
Fragments arranged by size:
1209 759 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1349 2149 2271
Size: 1243 106 800 122
Fragments arranged by size:
1243 800 122 106
Bpu10I CC'TnA_GC
Cuts at: 0 65 917 2200 2271
Size: 65 852 1283 71
Fragments arranged by size:
1283 852 71 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1360 2271
Size: 1220 140 911
Fragments arranged by size:
1220 911 140
BsaAI yAC'GTr
Cuts at: 0 179 2271
Size: 179 2092
BsaHI Gr'CG_yC
Cuts at: 0 230 1317 1738 2111 2271
Size: 230 1087 421 373 160
Fragments arranged by size:
1087 421 373 230 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1429 1459 1856 1886 2026 2056 2271
Size: 1429 30 397 30 140 30 215
Fragments arranged by size:
1429 397 215 140 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 477 826 1041 1051 1321 1804 1880 1918
Size: 477 349 215 10 270 483 76 38
Cuts at: 1918 2271
Size: 353
Fragments arranged by size:
483 477 353 349 270 215 76 38
10
BseYI C'CCAG_C
Cuts at: 0 135 776 864 1750 2263 2271
Size: 135 641 88 886 513 8
Fragments arranged by size:
886 641 513 135 88 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1381 2271
Size: 1120 261 890
Fragments arranged by size:
1120 890 261
BsiHKAI G_wGCw'C
Cuts at: 0 331 929 1229 2234 2271
Size: 331 598 300 1005 37
Fragments arranged by size:
1005 598 331 300 37
BsmI GAATG_Cn'
Cuts at: 0 78 192 1850 2271
Size: 78 114 1658 421
Fragments arranged by size:
1658 421 114 78
BsmBI CGTCTCn'nnnn_
Cuts at: 0 745 2271
Size: 745 1526
Bsp1286I G_dGCh'C
Cuts at: 0 331 415 451 565 800 929 1229 1675
Size: 331 84 36 114 235 129 300 446
Cuts at: 1675 1777 1919 2234 2271
Size: 102 142 315 37
Fragments arranged by size:
446 331 315 300 235 142 129 114
102 84 37 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1149 1275 2264 2271
Size: 1149 126 989 7
Fragments arranged by size:
1149 989 126 7
BsrBI CCG'CTC
Cuts at: 0 1188 2271
Size: 1188 1083
BsrDI GCAATG_nn'
Cuts at: 0 324 1304 1898 2271
Size: 324 980 594 373
Fragments arranged by size:
980 594 373 324
BsrFI r'CCGG_y
Cuts at: 0 523 1813 2212 2271
Size: 523 1290 399 59
Fragments arranged by size:
1290 523 399 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1476 2271
Size: 1476 795
BstEII G'GTnAC_C
Cuts at: 0 238 1809 2271
Size: 238 1571 462
Fragments arranged by size:
1571 462 238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 852 2271
Size: 624 228 1419
Fragments arranged by size:
1419 624 228
BtgI C'CryG_G
Cuts at: 0 1448 1805 1844 2271
Size: 1448 357 39 427
Fragments arranged by size:
1448 427 357 39
BtsI GCAGTG_nn'
Cuts at: 0 98 1064 1762 1889 2271
Size: 98 966 698 127 382
Fragments arranged by size:
966 698 382 127 98
ClaI AT'CG_AT
Cuts at: 0 879 2271
Size: 879 1392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 150 185 2271
Size: 150 35 2086
Fragments arranged by size:
2086 150 35
DraIII CAC_nnn'GTG
Cuts at: 0 729 2271
Size: 729 1542
DrdI GACnn_nn'nnGTC
Cuts at: 0 1546 2271
Size: 1546 725
EaeI y'GGCC_r
Cuts at: 0 306 378 420 677 748 1301 1451 2271
Size: 306 72 42 257 71 553 150 820
Fragments arranged by size:
820 553 306 257 150 72 71 42
EarI CTCTTCn'nnn_
Cuts at: 0 16 319 1178 2271
Size: 16 303 859 1093
Fragments arranged by size:
1093 859 303 16
EcoICRI GAG'CTC
Cuts at: 0 1227 2232 2271
Size: 1227 1005 39
Fragments arranged by size:
1227 1005 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 983 1243 1349 2149 2164 2271
Size: 592 391 260 106 800 15 107
Fragments arranged by size:
800 592 391 260 107 106 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 502 965 2131 2271
Size: 442 60 463 1166 140
Fragments arranged by size:
1166 463 442 140 60
EcoO109I rG'GnC_Cy
Cuts at: 0 140 1672 1838 1976 2081 2271
Size: 140 1532 166 138 105 190
Fragments arranged by size:
1532 190 166 140 138 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 2271
Size: 735 1536
HaeII r_GCGC'y
Cuts at: 0 161 977 1320 1989 2271
Size: 161 816 343 669 282
Fragments arranged by size:
816 669 343 282 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 723 755 1429 1461 2073 2105 2271
Size: 723 32 674 32 612 32 166
Fragments arranged by size:
723 674 612 166 32 32 32
HincII GTy'rAC
Cuts at: 0 1292 2109 2271
Size: 1292 817 162
Fragments arranged by size:
1292 817 162
KasI G'GCGC_C
Cuts at: 0 1316 2271
Size: 1316 955
MfeI C'AATT_G
Cuts at: 0 390 2271
Size: 390 1881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1842 2069 2201 2271
Size: 1842 227 132 70
Fragments arranged by size:
1842 227 132 70
MscI TGG'CCA
Cuts at: 0 380 422 679 2271
Size: 380 42 257 1592
Fragments arranged by size:
1592 380 257 42
MslI CAynn'nnrTG
Cuts at: 0 292 697 1557 1654 2271
Size: 292 405 860 97 617
Fragments arranged by size:
860 617 405 292 97
MspA1I CmG'CkG
Cuts at: 0 135 486 632 776 1221 1442 2271
Size: 135 351 146 144 445 221 829
Fragments arranged by size:
829 445 351 221 146 144 135
NaeI GCC'GGC
Cuts at: 0 525 2271
Size: 525 1746
NarI GG'CG_CC
Cuts at: 0 1317 2271
Size: 1317 954
NcoI C'CATG_G
Cuts at: 0 1805 1844 2271
Size: 1805 39 427
Fragments arranged by size:
1805 427 39
NgoMIV G'CCGG_C
Cuts at: 0 523 2271
Size: 523 1748
NsiI A_TGCA'T
Cuts at: 0 78 786 2271
Size: 78 708 1485
Fragments arranged by size:
1485 708 78
NspI r_CATG'y
Cuts at: 0 960 1434 1545 1758 2005 2241 2271
Size: 960 474 111 213 247 236 30
Fragments arranged by size:
960 474 247 236 213 111 30
PasI CC'CwG_GG
Cuts at: 0 951 2271
Size: 951 1320
PciI A'CATG_T
Cuts at: 0 1430 2237 2271
Size: 1430 807 34
Fragments arranged by size:
1430 807 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 350 1995 2271
Size: 350 1645 276
Fragments arranged by size:
1645 350 276
PfoI T'CCnGG_A
Cuts at: 0 149 1610 1834 2271
Size: 149 1461 224 437
Fragments arranged by size:
1461 437 224 149
PpuMI rG'GwC_Cy
Cuts at: 0 1838 2081 2271
Size: 1838 243 190
Fragments arranged by size:
1838 243 190
PsiI TTA'TAA
Cuts at: 0 1385 2271
Size: 1385 886
PspOMI G'GGCC_C
Cuts at: 0 1671 2271
Size: 1671 600
PstI C_TGCA'G
Cuts at: 0 1059 1472 2021 2053 2261 2271
Size: 1059 413 549 32 208 10
Fragments arranged by size:
1059 549 413 208 32 10
PvuII CAG'CTG
Cuts at: 0 135 486 632 1221 2271
Size: 135 351 146 589 1050
Fragments arranged by size:
1050 589 351 146 135
RsrII CG'GwC_CG
Cuts at: 0 1086 2271
Size: 1086 1185
SacI G_AGCT'C
Cuts at: 0 1229 2234 2271
Size: 1229 1005 37
Fragments arranged by size:
1229 1005 37
SalI G'TCGA_C
Cuts at: 0 1290 2271
Size: 1290 981
SapI GCTCTTCn'nnn_
Cuts at: 0 16 319 1178 2271
Size: 16 303 859 1093
Fragments arranged by size:
1093 859 303 16
SexAI A'CCwGG_T
Cuts at: 0 1712 2271
Size: 1712 559
SfcI C'TryA_G
Cuts at: 0 1055 1468 2017 2049 2257 2271
Size: 1055 413 549 32 208 14
Fragments arranged by size:
1055 549 413 208 32 14
SfoI GGC'GCC
Cuts at: 0 1318 2271
Size: 1318 953
SmaI CCC'GGG
Cuts at: 0 2119 2271
Size: 2119 152
SmlI C'TyrA_G
Cuts at: 0 85 1235 1375 2271
Size: 85 1150 140 896
Fragments arranged by size:
1150 896 140 85
SphI G_CATG'C
Cuts at: 0 960 1758 2005 2271
Size: 960 798 247 266
Fragments arranged by size:
960 798 266 247
StuI AGG'CCT
Cuts at: 0 430 2271
Size: 430 1841
StyI C'CwwG_G
Cuts at: 0 1805 1844 1961 1967 2271
Size: 1805 39 117 6 304
Fragments arranged by size:
1805 304 117 39 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1211 1798 2271
Size: 1211 587 473
Fragments arranged by size:
1211 587 473
TatI w'GTAC_w
Cuts at: 0 803 1326 2271
Size: 803 523 945
Fragments arranged by size:
945 803 523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1856 1888 2271
Size: 1856 32 383
Fragments arranged by size:
1856 383 32
Tth111I GACn'n_nGTC
Cuts at: 0 1421 1714 2271
Size: 1421 293 557
Fragments arranged by size:
1421 557 293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 852 1842 2271
Size: 852 990 429
Fragments arranged by size:
990 852 429
XmaI C'CCGG_G
Cuts at: 0 2117 2271
Size: 2117 154
XmnI GAAnn'nnTTC
Cuts at: 0 221 989 1663 2271
Size: 221 768 674 608
Fragments arranged by size:
768 674 608 221
ZraI GAC'GTC
Cuts at: 0 231 2112 2271
Size: 231 1881 159
Fragments arranged by size:
1881 231 159
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AleI AlfI
AlwNI ApaI ApoI AvaI BaeI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BtgI BtsI ClaI CspCI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PpuMI PsiI PspOMI PstI
PvuII RsrII SacI SalI SapI SexAI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AloI ApaLI AscI
AseI AsiSI AvrII BamHI BciVI BglI BglII BlpI
BmgBI BmtI BsaI BsaBI BsaWI BsiEI BsiWI BspEI
BspHI BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I
BtgZI DraI EagI EciI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MluI NdeI NheI NotI
NruI PacI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI SacII SanDI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI