Restriction map for
pF1KSDA0216
(Linear) (Six-base) MAPSORT of: KIAA0216.seq Check: 1374 from: 1 to: 6162
KIAA0216.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4090 5159 6162
Size: 4090 1069 1003
Fragments arranged by size:
4090 1069 1003
AccI GT'mk_AC
Cuts at: 0 608 2363 6162
Size: 608 1755 3799
Fragments arranged by size:
3799 1755 608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2066 4027 4138 4513 4993 5044 5194 6162
Size: 2066 1961 111 375 480 51 150 968
Fragments arranged by size:
2066 1961 968 480 375 150 111 51
AfeI AGC'GCT
Cuts at: 0 412 1282 2173 5186 6162
Size: 412 870 891 3013 976
Fragments arranged by size:
3013 976 891 870 412
AflIII A'CryG_T
Cuts at: 0 1304 1379 6162
Size: 1304 75 4783
Fragments arranged by size:
4783 1304 75
AhdI GACnn_n'nnGTC
Cuts at: 0 4944 6162
Size: 4944 1218
AleI CACnn'nnGTG
Cuts at: 0 2156 2247 2482 6162
Size: 2156 91 235 3680
Fragments arranged by size:
3680 2156 235 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 1325 2265 2500 5306 6162
Size: 733 592 940 235 2806 856
Fragments arranged by size:
2806 940 856 733 592 235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 5071 5103 6162
Size: 136 32 4903 32 1059
Fragments arranged by size:
4903 1059 136 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 956 1530 1542 1602 2595 3488 3645 3795
Size: 956 574 12 60 993 893 157 150
Cuts at: 3795 3933 4302 4701 5376 5751 5992 6032 6162
Size: 138 369 399 675 375 241 40 130
Fragments arranged by size:
993 956 893 675 574 399 375 369
241 157 150 138 130 60 40 12
ApaI G_GGCC'C
Cuts at: 0 733 1350 2085 2197 6162
Size: 733 617 735 112 3965
Fragments arranged by size:
3965 735 733 617 112
ApaLI G'TGCA_C
Cuts at: 0 2240 3130 6162
Size: 2240 890 3032
Fragments arranged by size:
3032 2240 890
AseI AT'TA_AT
Cuts at: 0 836 1646 6162
Size: 836 810 4516
Fragments arranged by size:
4516 836 810
AvaI C'yCGr_G
Cuts at: 0 279 572 733 2197 2370 3225 3808 4231
Size: 279 293 161 1464 173 855 583 423
Cuts at: 4231 5309 6162
Size: 1078 853
Fragments arranged by size:
1464 1078 855 853 583 423 293 279
173 161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1225 1258 6162
Size: 1225 33 4904
Fragments arranged by size:
4904 1225 33
BanI G'GyrC_C
Cuts at: 0 563 630 784 810 1410 1760 1819 2161
Size: 563 67 154 26 600 350 59 342
Cuts at: 2161 2664 2800 3523 6162
Size: 503 136 723 2639
Fragments arranged by size:
2639 723 600 563 503 350 342 154
136 67 59 26
BanII G_rGCy'C
Cuts at: 0 579 733 938 947 1112 1350 1838 2085
Size: 579 154 205 9 165 238 488 247
Cuts at: 2085 2197 2288 2322 3173 3251 4272 4400 4568
Size: 112 91 34 851 78 1021 128 168
Cuts at: 4568 6162
Size: 1594
Fragments arranged by size:
1594 1021 851 579 488 247 238 205
168 165 154 128 112 91 78 34
9
BbeI G_GCGC'C
Cuts at: 0 1764 6162
Size: 1764 4398
BbsI GAAGACnn'nnnn_
Cuts at: 0 1782 5147 5264 6162
Size: 1782 3365 117 898
Fragments arranged by size:
3365 1782 898 117
BbvCI CC'TCA_GC
Cuts at: 0 1713 2130 3152 4478 4915 6162
Size: 1713 417 1022 1326 437 1247
Fragments arranged by size:
1713 1326 1247 1022 437 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2616 2650 6162
Size: 2616 34 3512
Fragments arranged by size:
3512 2616 34
BglI GCCn_nnn'nGGC
Cuts at: 0 737 2089 2258 2933 2975 4993 6162
Size: 737 1352 169 675 42 2018 1169
Fragments arranged by size:
2018 1352 1169 737 675 169 42
BlpI GC'TnA_GC
Cuts at: 0 770 937 946 1916 4310 5200 5342 6162
Size: 770 167 9 970 2394 890 142 820
Fragments arranged by size:
2394 970 890 820 770 167 142 9
Bme1580I G_kGCm'C
Cuts at: 0 566 635 733 845 1350 2085 2197 2244
Size: 566 69 98 112 505 735 112 47
Cuts at: 2244 3134 3269 3526 6162
Size: 890 135 257 2636
Fragments arranged by size:
2636 890 735 566 505 257 135 112
112 98 69 47
BmgBI CAC'GTC
Cuts at: 0 127 3264 6162
Size: 127 3137 2898
Fragments arranged by size:
3137 2898 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 598 2113 2817 3987 6162
Size: 598 1515 704 1170 2175
Fragments arranged by size:
2175 1515 1170 704 598
BmtI G_CTAG'C
Cuts at: 0 1295 5537 6162
Size: 1295 4242 625
Fragments arranged by size:
4242 1295 625
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 465 2145 2997 3939 5922 6162
Size: 465 1680 852 942 1983 240
Fragments arranged by size:
1983 1680 942 852 465 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 288 682 704 1240 1759 2137 2266
Size: 127 161 394 22 536 519 378 129
Cuts at: 2266 2713 2992 3478 3493 3559 3880 4150 4210
Size: 447 279 486 15 66 321 270 60
Cuts at: 4210 4357 4387 4552 4660 4744 4915 5095 5209
Size: 147 30 165 108 84 171 180 114
Cuts at: 5209 5263 5383 5440 5500 5512 5542 5731 5752
Size: 54 120 57 60 12 30 189 21
Cuts at: 5752 5947 6075 6162
Size: 195 128 87
Fragments arranged by size:
536 519 486 447 394 378 321 279
270 195 189 180 171 165 161 147
129 128 127 120 114 108 87 84
66 60 60 57 54 30 30 22
21 15 12
Bpu10I CC'TnA_GC
Cuts at: 0 581 1713 2130 2387 3152 3499 4478 4915
Size: 581 1132 417 257 765 347 979 437
Cuts at: 4915 5300 6162
Size: 385 862
Fragments arranged by size:
1132 979 862 765 581 437 417 385
347 257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 898 2275 2308 4267 5047 5527 5917
Size: 301 597 1377 33 1959 780 480 390
Cuts at: 5917 6162
Size: 245
Fragments arranged by size:
1959 1377 780 597 480 390 301 245
33
BsaI GGTCTCn'nnnn_
Cuts at: 0 3224 6162
Size: 3224 2938
BsaAI yAC'GTr
Cuts at: 0 1307 6162
Size: 1307 4855
BsaHI Gr'CG_yC
Cuts at: 0 463 1761 3089 6162
Size: 463 1298 1328 3073
Fragments arranged by size:
3073 1328 1298 463
BsaWI w'CCGG_w
Cuts at: 0 3767 6162
Size: 3767 2395
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 136 166 229 259 635 665
Size: 67 30 39 30 63 30 376 30
Cuts at: 665 844 874 1039 1069 2110 2140 2566 2596
Size: 179 30 165 30 1041 30 426 30
Cuts at: 2596 5071 5101 5381 5411 6073 6103 6162
Size: 2475 30 280 30 662 30 59
Fragments arranged by size:
2475 1041 662 426 376 280 179 165
67 63 59 39 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 141 340 1120 2134 2215 2437 2524
Size: 124 17 199 780 1014 81 222 87
Cuts at: 2524 2668 3174 3463 3556 3772 3796 4270 4395
Size: 144 506 289 93 216 24 474 125
Cuts at: 4395 4429 4462 4798 4807 5092 5206 5584 5668
Size: 34 33 336 9 285 114 378 84
Cuts at: 5668 6162
Size: 494
Fragments arranged by size:
1014 780 506 494 474 378 336 289
285 222 216 199 144 125 124 114
93 87 84 81 34 33 24 17
9
BseYI C'CCAG_C
Cuts at: 0 425 1318 1764 1953 2050 2856 3158 3163
Size: 425 893 446 189 97 806 302 5
Cuts at: 3163 3244 3281 3926 4421 5003 5038 5057 6162
Size: 81 37 645 495 582 35 19 1105
Fragments arranged by size:
1105 893 806 645 582 495 446 425
302 189 97 81 37 35 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 550 800 940 1249 1285 1555 2506
Size: 211 339 250 140 309 36 270 951
Cuts at: 2506 2740 4315 4858 5131 6162
Size: 234 1575 543 273 1031
Fragments arranged by size:
1575 1031 951 543 339 309 273 270
250 234 211 140 36
BsiEI CG_ry'CG
Cuts at: 0 1215 2270 6162
Size: 1215 1055 3892
Fragments arranged by size:
3892 1215 1055
BsiHKAI G_wGCw'C
Cuts at: 0 476 938 947 1112 1838 2244 2288 2951
Size: 476 462 9 165 726 406 44 663
Cuts at: 2951 3134 4400 4568 5037 6071 6162
Size: 183 1266 168 469 1034 91
Fragments arranged by size:
1266 1034 726 663 476 469 462 406
183 168 165 91 44 9
BsmI GAATG_Cn'
Cuts at: 0 2874 5773 6162
Size: 2874 2899 389
Fragments arranged by size:
2899 2874 389
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1036 2307 4057 5545 6162
Size: 1036 1271 1750 1488 617
Fragments arranged by size:
1750 1488 1271 1036 617
Bsp1286I G_dGCh'C
Cuts at: 0 476 566 579 635 733 845 938 947
Size: 476 90 13 56 98 112 93 9
Cuts at: 947 1112 1350 1838 2085 2197 2244 2288 2322
Size: 165 238 488 247 112 47 44 34
Cuts at: 2322 2951 3134 3173 3251 3269 3526 4272 4400
Size: 629 183 39 78 18 257 746 128
Cuts at: 4400 4568 5037 6071 6162
Size: 168 469 1034 91
Fragments arranged by size:
1034 746 629 488 476 469 257 247
238 183 168 165 128 112 112 98
93 91 90 78 56 47 44 39
34 18 13 9
BspEI T'CCGG_A
Cuts at: 0 3767 6162
Size: 3767 2395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 620 1808 3227 4090 4247 4338 5153
Size: 233 387 1188 1419 863 157 91 815
Cuts at: 5153 5159 5801 6162
Size: 6 642 361
Fragments arranged by size:
1419 1188 863 815 642 387 361 233
157 91 6
BsrBI CCG'CTC
Cuts at: 0 78 149 968 2603 4128 4740 5574 6162
Size: 78 71 819 1635 1525 612 834 588
Fragments arranged by size:
1635 1525 834 819 612 588 78 71
BsrDI GCAATG_nn'
Cuts at: 0 1659 4523 5129 5585 6162
Size: 1659 2864 606 456 577
Fragments arranged by size:
2864 1659 606 577 456
BsrFI r'CCGG_y
Cuts at: 0 2269 2450 3854 4931 5237 6162
Size: 2269 181 1404 1077 306 925
Fragments arranged by size:
2269 1404 1077 925 306 181
BsrGI T'GTAC_A
Cuts at: 0 1604 3660 6162
Size: 1604 2056 2502
Fragments arranged by size:
2502 2056 1604
BssSI C'ACGA_G
Cuts at: 0 2470 6162
Size: 2470 3692
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1272 1533 2938 3129 3488 5376 6162
Size: 1272 261 1405 191 359 1888 786
Fragments arranged by size:
1888 1405 1272 786 359 261 191
BstEII G'GTnAC_C
Cuts at: 0 3331 6162
Size: 3331 2831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 207 795 1285 1326 2797 3036 6162
Size: 207 588 490 41 1471 239 3126
Fragments arranged by size:
3126 1471 588 490 239 207 41
BstYI r'GATC_y
Cuts at: 0 1224 1673 3468 3635 3764 3785 3869 5228
Size: 1224 449 1795 167 129 21 84 1359
Cuts at: 5228 5718 6162
Size: 490 444
Fragments arranged by size:
1795 1359 1224 490 449 444 167 129
84 21
Bsu36I CC'TnA_GG
Cuts at: 0 1121 1825 1895 6162
Size: 1121 704 70 4267
Fragments arranged by size:
4267 1121 704 70
BtgI C'CryG_G
Cuts at: 0 339 626 1341 2406 2796 2942 3288 5294
Size: 339 287 715 1065 390 146 346 2006
Cuts at: 5294 5429 6162
Size: 135 733
Fragments arranged by size:
2006 1065 733 715 390 346 339 287
146 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 323 1465 1536 2946 5151 6162
Size: 323 1142 71 1410 2205 1011
Fragments arranged by size:
2205 1410 1142 1011 323 71
BtsI GCAGTG_nn'
Cuts at: 0 1433 1509 2943 2992 3132 3213 3234 3449
Size: 1433 76 1434 49 140 81 21 215
Cuts at: 3449 6162
Size: 2713
Fragments arranged by size:
2713 1434 1433 215 140 81 76 49
21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5111 5146 6162
Size: 5111 35 1016
Fragments arranged by size:
5111 1016 35
DraIII CAC_nnn'GTG
Cuts at: 0 849 3348 6162
Size: 849 2499 2814
Fragments arranged by size:
2814 2499 849
EaeI y'GGCC_r
Cuts at: 0 209 380 1085 1544 1701 1886 1940 2267
Size: 209 171 705 459 157 185 54 327
Cuts at: 2267 3647 4610 6162
Size: 1380 963 1552
Fragments arranged by size:
1552 1380 963 705 459 327 209 185
171 157 54
EagI C'GGCC_G
Cuts at: 0 2267 6162
Size: 2267 3895
EarI CTCTTCn'nnn_
Cuts at: 0 1022 2293 2470 2645 3472 3622 4661 4844
Size: 1022 1271 177 175 827 150 1039 183
Cuts at: 4844 6162
Size: 1318
Fragments arranged by size:
1318 1271 1039 1022 827 183 177 175
150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 57 2176 3168 3352 3832 3909 6162
Size: 57 2119 992 184 480 77 2253
Fragments arranged by size:
2253 2119 992 480 184 77 57
EcoICRI GAG'CTC
Cuts at: 0 936 945 1110 1836 2286 4398 4566 6162
Size: 936 9 165 726 450 2112 168 1596
Fragments arranged by size:
2112 1596 936 726 450 168 165 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 288 682 704 1240 1759 2066 2137
Size: 127 161 394 22 536 519 307 71
Cuts at: 2137 2266 2713 2992 3478 3493 3559 3880 4027
Size: 129 447 279 486 15 66 321 147
Cuts at: 4027 4138 4150 4210 4357 4387 4513 4552 4660
Size: 111 12 60 147 30 126 39 108
Cuts at: 4660 4744 4915 4993 5044 5095 5194 5209 5263
Size: 84 171 78 51 51 99 15 54
Cuts at: 5263 5383 5440 5500 5512 5542 5731 5752 5947
Size: 120 57 60 12 30 189 21 195
Cuts at: 5947 6075 6162
Size: 128 87
Fragments arranged by size:
536 519 486 447 394 321 307 279
195 189 171 161 147 147 129 128
127 126 120 111 108 99 87 84
78 71 66 60 60 57 54 51
51 39 30 30 22 21 15 15
12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 542 557 779 4477 6162
Size: 542 15 222 3698 1685
Fragments arranged by size:
3698 1685 542 222 15
EcoO109I rG'GnC_Cy
Cuts at: 0 729 730 792 976 1346 1347 2193 2194
Size: 729 1 62 184 370 1 846 1
Cuts at: 2194 2631 3642 3731 4236 4635 4968 4986 5313
Size: 437 1011 89 505 399 333 18 327
Cuts at: 5313 5790 5838 5974 6162
Size: 477 48 136 188
Fragments arranged by size:
1011 846 729 505 477 437 399 370
333 327 188 184 136 89 62 48
18 1 1 1
FseI GG_CCGG'CC
Cuts at: 0 2273 6162
Size: 2273 3889
FspI TGC'GCA
Cuts at: 0 3316 6162
Size: 3316 2846
FspAI rTGC'GCAy
Cuts at: 0 3316 6162
Size: 3316 2846
HaeII r_GCGC'y
Cuts at: 0 414 449 1284 1292 1764 2175 2700 5188
Size: 414 35 835 8 472 411 525 2488
Cuts at: 5188 6162
Size: 974
Fragments arranged by size:
2488 974 835 525 472 414 411 35
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 229 261 844 876 1063 1095
Size: 67 32 130 32 583 32 187 32
Cuts at: 1095 1216 1248 1449 1481 2110 2142 2566 2598
Size: 121 32 201 32 629 32 424 32
Cuts at: 2598 3208 3240 3868 3900 4758 4790 4930 4962
Size: 610 32 628 32 858 32 140 32
Cuts at: 4962 5316 5348 5859 5891 5902 5934 6073 6105
Size: 354 32 511 32 11 32 139 32
Cuts at: 6105 6162
Size: 57
Fragments arranged by size:
858 629 628 610 583 511 424 354
201 187 140 139 130 121 67 57
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
11
HincII GTy'rAC
Cuts at: 0 609 1093 2364 5940 6162
Size: 609 484 1271 3576 222
Fragments arranged by size:
3576 1271 609 484 222
HindIII A'AGCT_T
Cuts at: 0 3712 6162
Size: 3712 2450
KasI G'GCGC_C
Cuts at: 0 1760 6162
Size: 1760 4402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 2474 6162
Size: 212 2262 3688
Fragments arranged by size:
3688 2262 212
MscI TGG'CCA
Cuts at: 0 211 382 1087 1546 1703 1888 3649 4612
Size: 211 171 705 459 157 185 1761 963
Cuts at: 4612 6162
Size: 1550
Fragments arranged by size:
1761 1550 963 705 459 211 185 171
157
MslI CAynn'nnrTG
Cuts at: 0 205 715 1307 1421 1948 2156 2247 2482
Size: 205 510 592 114 527 208 91 235
Cuts at: 2482 2795 3010 3034 3070 3316 3424 4696 6162
Size: 313 215 24 36 246 108 1272 1466
Fragments arranged by size:
1466 1272 592 527 510 313 246 235
215 208 205 114 108 91 36 24
MspA1I CmG'CkG
Cuts at: 0 88 215 357 525 1520 1565 2408 2988
Size: 88 127 142 168 995 45 843 580
Cuts at: 2988 3290 3485 3549 3750 3795 3930 4066 4218
Size: 302 195 64 201 45 135 136 152
Cuts at: 4218 4266 4425 4440 4455 5073 5199 6162
Size: 48 159 15 15 618 126 963
Fragments arranged by size:
995 963 843 618 580 302 201 195
168 159 152 142 136 135 127 126
88 64 48 45 45 15 15
NaeI GCC'GGC
Cuts at: 0 2271 6162
Size: 2271 3891
NarI GG'CG_CC
Cuts at: 0 1761 6162
Size: 1761 4401
NcoI C'CATG_G
Cuts at: 0 2796 5429 6162
Size: 2796 2633 733
Fragments arranged by size:
2796 2633 733
NgoMIV G'CCGG_C
Cuts at: 0 2269 6162
Size: 2269 3893
NheI G'CTAG_C
Cuts at: 0 1291 5533 6162
Size: 1291 4242 629
Fragments arranged by size:
4242 1291 629
NspI r_CATG'y
Cuts at: 0 1383 3015 3321 4470 6162
Size: 1383 1632 306 1149 1692
Fragments arranged by size:
1692 1632 1383 1149 306
PasI CC'CwG_GG
Cuts at: 0 801 6162
Size: 801 5361
PciI A'CATG_T
Cuts at: 0 1379 6162
Size: 1379 4783
PflMI CCAn_nnn'nTGG
Cuts at: 0 1530 3645 3933 4365 4701 6032 6162
Size: 1530 2115 288 432 336 1331 130
Fragments arranged by size:
2115 1530 1331 432 336 288 130
PfoI T'CCnGG_A
Cuts at: 0 272 653 1163 1613 2690 3638 4568 4631
Size: 272 381 510 450 1077 948 930 63
Cuts at: 4631 5417 5639 6162
Size: 786 222 523
Fragments arranged by size:
1077 948 930 786 523 510 450 381
272 222 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 4214 4246 5071 5103 6162
Size: 136 32 4046 32 825 32 1059
Fragments arranged by size:
4046 1059 825 136 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 792 976 3642 4236 4635 4968 5313 5790
Size: 792 184 2666 594 399 333 345 477
Cuts at: 5790 5838 5974 6162
Size: 48 136 188
Fragments arranged by size:
2666 792 594 477 399 345 333 188
184 136 48
PspOMI G'GGCC_C
Cuts at: 0 729 1346 2081 2193 6162
Size: 729 617 735 112 3969
Fragments arranged by size:
3969 735 729 617 112
PstI C_TGCA'G
Cuts at: 0 356 1937 2113 2171 2459 2888 3257 3598
Size: 356 1581 176 58 288 429 369 341
Cuts at: 3598 3989 4244 4277 4439 4454 5156 5381 5756
Size: 391 255 33 162 15 702 225 375
Cuts at: 5756 5798 6162
Size: 42 364
Fragments arranged by size:
1581 702 429 391 375 369 364 356
341 288 255 225 176 162 58 42
33 15
PvuII CAG'CTG
Cuts at: 0 525 1520 2988 3485 3750 3795 3930 4218
Size: 525 995 1468 497 265 45 135 288
Cuts at: 4218 5073 5199 6162
Size: 855 126 963
Fragments arranged by size:
1468 995 963 855 525 497 288 265
135 126 45
SacI G_AGCT'C
Cuts at: 0 938 947 1112 1838 2288 4400 4568 6162
Size: 938 9 165 726 450 2112 168 1594
Fragments arranged by size:
2112 1594 938 726 450 168 165 9
SacII CC_GC'GG
Cuts at: 0 2409 3291 6162
Size: 2409 882 2871
Fragments arranged by size:
2871 2409 882
SalI G'TCGA_C
Cuts at: 0 607 2362 6162
Size: 607 1755 3800
Fragments arranged by size:
3800 1755 607
SapI GCTCTTCn'nnn_
Cuts at: 0 2293 2470 3472 4661 6162
Size: 2293 177 1002 1189 1501
Fragments arranged by size:
2293 1501 1189 1002 177
SbfI CC_TGCA'GG
Cuts at: 0 2888 3598 4244 5756 5798 6162
Size: 2888 710 646 1512 42 364
Fragments arranged by size:
2888 1512 710 646 364 42
SexAI A'CCwGG_T
Cuts at: 0 4922 6162
Size: 4922 1240
SfcI C'TryA_G
Cuts at: 0 352 1933 2109 2167 2277 2455 2884 3079
Size: 352 1581 176 58 110 178 429 195
Cuts at: 3079 3146 3253 3594 3985 4240 4273 4435 4450
Size: 67 107 341 391 255 33 162 15
Cuts at: 4450 5152 5377 5626 5752 5794 6162
Size: 702 225 249 126 42 368
Fragments arranged by size:
1581 702 429 391 368 352 341 255
249 225 195 178 176 162 126 110
107 67 58 42 33 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 737 6162
Size: 737 5425
SfoI GGC'GCC
Cuts at: 0 1762 6162
Size: 1762 4400
SmaI CCC'GGG
Cuts at: 0 2372 5311 6162
Size: 2372 2939 851
Fragments arranged by size:
2939 2372 851
SmlI C'TyrA_G
Cuts at: 0 316 913 2254 2323 3808 4282 5062 5542
Size: 316 597 1341 69 1485 474 780 480
Cuts at: 5542 5896 6162
Size: 354 266
Fragments arranged by size:
1485 1341 780 597 480 474 354 316
266 69
SphI G_CATG'C
Cuts at: 0 3015 6162
Size: 3015 3147
StuI AGG'CCT
Cuts at: 0 745 1033 1981 2581 6162
Size: 745 288 948 600 3581
Fragments arranged by size:
3581 948 745 600 288
StyI C'CwwG_G
Cuts at: 0 788 2796 3327 3527 4592 4613 4982 5429
Size: 788 2008 531 200 1065 21 369 447
Cuts at: 5429 5648 5981 6162
Size: 219 333 181
Fragments arranged by size:
2008 1065 788 531 447 369 333 219
200 181 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2458 2878 3295 4552 6044 6162
Size: 2458 420 417 1257 1492 118
Fragments arranged by size:
2458 1492 1257 420 417 118
TatI w'GTAC_w
Cuts at: 0 292 1358 1604 2816 3660 3744 6162
Size: 292 1066 246 1212 844 84 2418
Fragments arranged by size:
2418 1212 1066 844 292 246 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 512 544 635 667 1039 1071 1119 1151
Size: 512 32 91 32 372 32 48 32
Cuts at: 1151 3050 3082 6162
Size: 1899 32 3080
Fragments arranged by size:
3080 1899 512 372 91 48 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 5944 6162
Size: 5944 218
XbaI T'CTAG_A
Cuts at: 0 513 4554 5721 6162
Size: 513 4041 1167 441
Fragments arranged by size:
4041 1167 513 441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 1167 1950 2010 2155 2967 3241 4332
Size: 174 993 783 60 145 812 274 1091
Cuts at: 4332 4698 4719 4989 5184 5655 6162
Size: 366 21 270 195 471 507
Fragments arranged by size:
1091 993 812 783 507 471 366 274
270 195 174 145 60 21
XhoI C'TCGA_G
Cuts at: 0 3808 6162
Size: 3808 2354
XmaI C'CCGG_G
Cuts at: 0 2370 5309 6162
Size: 2370 2939 853
Fragments arranged by size:
2939 2370 853
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AseI AvaI BaeI BanI
BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI FspAI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SalI SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI ApoI AscI AsiSI
AvrII BamHI BciVI BclI BfrBI BglII BsaBI BsiWI
BspHI BssHII BstBI BstZ17I ClaI DraI DrdI EcoRI
EcoRV FalI HpaI KpnI MfeI MluI NdeI NotI
NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SanDI ScaI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XmnI ZraI