Restriction map for
pF1KSDA0217
(Linear) (Six-base) MAPSORT of: KIAA0217.seq Check: 2391 from: 1 to: 2214
KIAA0217.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1899 2214
Size: 1899 315
AatII G_ACGT'C
Cuts at: 0 1018 2214
Size: 1018 1196
Acc65I G'GTAC_C
Cuts at: 0 141 2214
Size: 141 2073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 2214
Size: 1385 829
AfeI AGC'GCT
Cuts at: 0 72 1006 2214
Size: 72 934 1208
Fragments arranged by size:
1208 934 72
AflIII A'CryG_T
Cuts at: 0 527 2214
Size: 527 1687
AleI CACnn'nnGTG
Cuts at: 0 577 616 2214
Size: 577 39 1598
Fragments arranged by size:
1598 577 39
AlwNI CAG_nnn'CTG
Cuts at: 0 847 2214
Size: 847 1367
ApoI r'AATT_y
Cuts at: 0 493 787 802 1488 2214
Size: 493 294 15 686 726
Fragments arranged by size:
726 686 493 294 15
AseI AT'TA_AT
Cuts at: 0 1333 1337 2214
Size: 1333 4 877
Fragments arranged by size:
1333 877 4
AvaI C'yCGr_G
Cuts at: 0 467 2214
Size: 467 1747
BanI G'GyrC_C
Cuts at: 0 141 1755 2214
Size: 141 1614 459
Fragments arranged by size:
1614 459 141
BanII G_rGCy'C
Cuts at: 0 195 1751 2066 2087 2115 2214
Size: 195 1556 315 21 28 99
Fragments arranged by size:
1556 315 195 99 28 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 467 1639 2214
Size: 467 1172 575
Fragments arranged by size:
1172 575 467
BciVI GTATCCnnnnn_n'
Cuts at: 0 1226 2214
Size: 1226 988
BclI T'GATC_A
Cuts at: 0 9 1073 2214
Size: 9 1064 1141
Fragments arranged by size:
1141 1064 9
BglII A'GATC_T
Cuts at: 0 643 2214
Size: 643 1571
BlpI GC'TnA_GC
Cuts at: 0 607 2214
Size: 607 1607
BmgBI CAC'GTC
Cuts at: 0 984 1088 2214
Size: 984 104 1126
Fragments arranged by size:
1126 984 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1154 1634 1725 2129 2214
Size: 1154 480 91 404 85
Fragments arranged by size:
1154 480 404 91 85
Bpu10I CC'TnA_GC
Cuts at: 0 20 2214
Size: 20 2194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2080 2214
Size: 2080 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 1743 2114 2214
Size: 1743 371 100
Fragments arranged by size:
1743 371 100
BsaHI Gr'CG_yC
Cuts at: 0 1015 1189 2214
Size: 1015 174 1025
Fragments arranged by size:
1025 1015 174
BsaWI w'CCGG_w
Cuts at: 0 1346 1502 2214
Size: 1346 156 712
Fragments arranged by size:
1346 712 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 668 698 1507 1530 1537 1560 2214
Size: 668 30 809 23 7 23 654
Fragments arranged by size:
809 668 654 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 1184 1211 1531 2198 2214
Size: 265 919 27 320 667 16
Fragments arranged by size:
919 667 320 265 27 16
BseYI C'CCAG_C
Cuts at: 0 360 998 1046 1423 1584 1803 1942 2088
Size: 360 638 48 377 161 219 139 146
Cuts at: 2088 2214
Size: 126
Fragments arranged by size:
638 377 360 219 161 146 139 126
48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1292 1724 1927 2214
Size: 1292 432 203 287
Fragments arranged by size:
1292 432 287 203
BsiEI CG_ry'CG
Cuts at: 0 1892 2134 2214
Size: 1892 242 80
Fragments arranged by size:
1892 242 80
BsiHKAI G_wGCw'C
Cuts at: 0 2194 2214
Size: 2194 20
BsiWI C'GTAC_G
Cuts at: 0 1784 2214
Size: 1784 430
BsmI GAATG_Cn'
Cuts at: 0 333 2214
Size: 333 1881
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1172 1196 2179 2214
Size: 1172 24 983 35
Fragments arranged by size:
1172 983 35 24
Bsp1286I G_dGCh'C
Cuts at: 0 195 1751 2066 2087 2115 2194 2214
Size: 195 1556 315 21 28 79 20
Fragments arranged by size:
1556 315 195 79 28 21 20
BspEI T'CCGG_A
Cuts at: 0 1346 2214
Size: 1346 868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1360 1899 2214
Size: 1360 539 315
Fragments arranged by size:
1360 539 315
BsrDI GCAATG_nn'
Cuts at: 0 334 1993 2214
Size: 334 1659 221
Fragments arranged by size:
1659 334 221
BsrFI r'CCGG_y
Cuts at: 0 1621 1758 2133 2144 2214
Size: 1621 137 375 11 70
Fragments arranged by size:
1621 375 137 70 11
BsrGI T'GTAC_A
Cuts at: 0 1037 1064 2214
Size: 1037 27 1150
Fragments arranged by size:
1150 1037 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 220 2214
Size: 220 1994
BstEII G'GTnAC_C
Cuts at: 0 310 2214
Size: 310 1904
BstYI r'GATC_y
Cuts at: 0 643 2214
Size: 643 1571
BtgI C'CryG_G
Cuts at: 0 276 743 1925 2162 2214
Size: 276 467 1182 237 52
Fragments arranged by size:
1182 467 276 237 52
BtsI GCAGTG_nn'
Cuts at: 0 225 1319 1883 2214
Size: 225 1094 564 331
Fragments arranged by size:
1094 564 331 225
ClaI AT'CG_AT
Cuts at: 0 1329 2214
Size: 1329 885
DraI TTT'AAA
Cuts at: 0 768 2214
Size: 768 1446
DraIII CAC_nnn'GTG
Cuts at: 0 1860 2214
Size: 1860 354
EaeI y'GGCC_r
Cuts at: 0 261 2131 2159 2214
Size: 261 1870 28 55
Fragments arranged by size:
1870 261 55 28
EagI C'GGCC_G
Cuts at: 0 2131 2214
Size: 2131 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1849 1866 2214
Size: 1849 17 348
Fragments arranged by size:
1849 348 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1154 1385 1634 1725 2129 2214
Size: 1154 231 249 91 404 85
Fragments arranged by size:
1154 404 249 231 91 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1469 2214
Size: 1469 745
EcoO109I rG'GnC_Cy
Cuts at: 0 388 1282 1694 2214
Size: 388 894 412 520
Fragments arranged by size:
894 520 412 388
EcoRI G'AATT_C
Cuts at: 0 493 1488 2214
Size: 493 995 726
Fragments arranged by size:
995 726 493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1509 2214
Size: 1509 705
FseI GG_CCGG'CC
Cuts at: 0 2148 2214
Size: 2148 66
HaeII r_GCGC'y
Cuts at: 0 74 1008 2214
Size: 74 934 1206
Fragments arranged by size:
1206 934 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 72 104 869 901 1530 1562 2214
Size: 26 46 32 765 32 629 32 652
Fragments arranged by size:
765 652 629 46 32 32 32 26
HindIII A'AGCT_T
Cuts at: 0 1575 2214
Size: 1575 639
KpnI G_GTAC'C
Cuts at: 0 145 2214
Size: 145 2069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 1573 2214
Size: 335 1238 641
Fragments arranged by size:
1238 641 335
MscI TGG'CCA
Cuts at: 0 263 2214
Size: 263 1951
MslI CAynn'nnrTG
Cuts at: 0 326 577 616 2214
Size: 326 251 39 1598
Fragments arranged by size:
1598 326 251 39
NaeI GCC'GGC
Cuts at: 0 1623 1760 2135 2146 2214
Size: 1623 137 375 11 68
Fragments arranged by size:
1623 375 137 68 11
NcoI C'CATG_G
Cuts at: 0 2162 2214
Size: 2162 52
NdeI CA'TA_TG
Cuts at: 0 1407 2214
Size: 1407 807
NgoMIV G'CCGG_C
Cuts at: 0 1621 1758 2133 2144 2214
Size: 1621 137 375 11 70
Fragments arranged by size:
1621 375 137 70 11
NspI r_CATG'y
Cuts at: 0 531 1261 2214
Size: 531 730 953
Fragments arranged by size:
953 730 531
PacI TTA_AT'TAA
Cuts at: 0 1337 2214
Size: 1337 877
PciI A'CATG_T
Cuts at: 0 527 2214
Size: 527 1687
PflMI CCAn_nnn'nTGG
Cuts at: 0 964 1144 1612 1860 2214
Size: 964 180 468 248 354
Fragments arranged by size:
964 468 354 248 180
PfoI T'CCnGG_A
Cuts at: 0 53 2214
Size: 53 2161
PpuMI rG'GwC_Cy
Cuts at: 0 388 1282 1694 2214
Size: 388 894 412 520
Fragments arranged by size:
894 520 412 388
PsiI TTA'TAA
Cuts at: 0 793 1153 2214
Size: 793 360 1061
Fragments arranged by size:
1061 793 360
PstI C_TGCA'G
Cuts at: 0 1326 2214
Size: 1326 888
SanDI GG'GwC_CC
Cuts at: 0 388 2214
Size: 388 1826
ScaI AGT'ACT
Cuts at: 0 476 2214
Size: 476 1738
SfcI C'TryA_G
Cuts at: 0 1322 1730 2214
Size: 1322 408 484
Fragments arranged by size:
1322 484 408
SmlI C'TyrA_G
Cuts at: 0 2095 2214
Size: 2095 119
SphI G_CATG'C
Cuts at: 0 1261 2214
Size: 1261 953
SspI AAT'ATT
Cuts at: 0 716 1310 2214
Size: 716 594 904
Fragments arranged by size:
904 716 594
StyI C'CwwG_G
Cuts at: 0 1121 1826 2162 2214
Size: 1121 705 336 52
Fragments arranged by size:
1121 705 336 52
TatI w'GTAC_w
Cuts at: 0 474 1037 1064 2214
Size: 474 563 27 1150
Fragments arranged by size:
1150 563 474 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1686 1718 2214
Size: 1686 32 496
Fragments arranged by size:
1686 496 32
Tth111I GACn'n_nGTC
Cuts at: 0 1089 1542 2214
Size: 1089 453 672
Fragments arranged by size:
1089 672 453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 274 2214
Size: 274 1940
ZraI GAC'GTC
Cuts at: 0 1016 2214
Size: 1016 1198
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI AlwNI
ApoI AseI AvaI BanI BanII BbsI BciVI BclI
BglII BlpI BmgBI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI
BstAPI BstEII BstYI BtgI BtsI ClaI DraI DraIII
EaeI EagI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI HaeII Hin4I HindIII KpnI MmeI MscI MslI
NaeI NcoI NdeI NgoMIV NspI PacI PciI PflMI
PfoI PpuMI PsiI PstI SanDI ScaI SfcI SmlI
SphI SspI StyI TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AlfI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BfrBI BglI Bme1580I BmrI BmtI BplI BsaAI
BsaBI BspHI BsrBI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgZI CspCI DrdI EarI EcoICRI EcoRV FspI
FspAI HincII HpaI KasI MfeI MluI MspA1I NarI
NheI NotI NruI NsiI PasI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII
XbaI XhoI XmaI XmnI