Restriction map for
pF1KA0219
(Linear) (Six-base) MAPSORT of: KIAA0219.seq Check: 6412 from: 1 to: 8013
KIAA0219.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 295 6575 7980 8013
Size: 295 6280 1405 33
Fragments arranged by size:
6280 1405 295 33
AccI GT'mk_AC
Cuts at: 0 2642 4605 8013
Size: 2642 1963 3408
Fragments arranged by size:
3408 2642 1963
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 858 1165 1586 1843 2114 2449 2683 2803
Size: 858 307 421 257 271 335 234 120
Cuts at: 2803 2974 3004 3161 3928 4258 6214 6379 7042
Size: 171 30 157 767 330 1956 165 663
Cuts at: 7042 7114 7394 7801 7975 8013
Size: 72 280 407 174 38
Fragments arranged by size:
1956 858 767 663 421 407 335 330
307 280 271 257 234 174 171 165
157 120 72 38 30
AflIII A'CryG_T
Cuts at: 0 4922 5809 7418 8013
Size: 4922 887 1609 595
Fragments arranged by size:
4922 1609 887 595
AleI CACnn'nnGTG
Cuts at: 0 981 2755 2984 6274 8013
Size: 981 1774 229 3290 1739
Fragments arranged by size:
3290 1774 1739 981 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 999 1043 2273 2321 6527 8013
Size: 223 776 44 1230 48 4206 1486
Fragments arranged by size:
4206 1486 1230 776 223 48 44
AlwNI CAG_nnn'CTG
Cuts at: 0 441 1143 1386 1504 1538 2550 2908 3171
Size: 441 702 243 118 34 1012 358 263
Cuts at: 3171 3346 4152 4155 4794 5146 5569 6120 6301
Size: 175 806 3 639 352 423 551 181
Cuts at: 6301 7221 8013
Size: 920 792
Fragments arranged by size:
1012 920 806 792 702 639 551 441
423 358 352 263 243 181 175 118
34 3
ApaI G_GGCC'C
Cuts at: 0 5330 8013
Size: 5330 2683
ApoI r'AATT_y
Cuts at: 0 769 1585 8013
Size: 769 816 6428
Fragments arranged by size:
6428 816 769
AvaI C'yCGr_G
Cuts at: 0 529 3070 5884 6272 6582 6979 8013
Size: 529 2541 2814 388 310 397 1034
Fragments arranged by size:
2814 2541 1034 529 397 388 310
AvrII C'CTAG_G
Cuts at: 0 7347 8013
Size: 7347 666
BamHI G'GATC_C
Cuts at: 0 4758 7562 8013
Size: 4758 2804 451
Fragments arranged by size:
4758 2804 451
BanI G'GyrC_C
Cuts at: 0 2111 2813 6443 7343 8013
Size: 2111 702 3630 900 670
Fragments arranged by size:
3630 2111 900 702 670
BanII G_rGCy'C
Cuts at: 0 149 575 1704 1967 2185 3154 3973 4314
Size: 149 426 1129 263 218 969 819 341
Cuts at: 4314 5128 5330 5786 8013
Size: 814 202 456 2227
Fragments arranged by size:
2227 1129 969 819 814 456 426 341
263 218 202 149
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 1831 2934 3118 4777 8013
Size: 275 1556 1103 184 1659 3236
Fragments arranged by size:
3236 1659 1556 1103 275 184
BbvCI CC'TCA_GC
Cuts at: 0 233 1517 3046 5162 7335 8013
Size: 233 1284 1529 2116 2173 678
Fragments arranged by size:
2173 2116 1529 1284 678 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1450 1484 1744 1778 8013
Size: 1450 34 260 34 6235
Fragments arranged by size:
6235 1450 260 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5353 8013
Size: 5353 2660
BclI T'GATC_A
Cuts at: 0 2012 8013
Size: 2012 6001
BglI GCCn_nnn'nGGC
Cuts at: 0 211 343 2733 4205 5044 7972 8013
Size: 211 132 2390 1472 839 2928 41
Fragments arranged by size:
2928 2390 1472 839 211 132 41
BglII A'GATC_T
Cuts at: 0 120 5850 8013
Size: 120 5730 2163
Fragments arranged by size:
5730 2163 120
BlpI GC'TnA_GC
Cuts at: 0 1160 1751 7294 8013
Size: 1160 591 5543 719
Fragments arranged by size:
5543 1160 719 591
Bme1580I G_kGCm'C
Cuts at: 0 741 1265 2346 2752 4622 5330 6023 6245
Size: 741 524 1081 406 1870 708 693 222
Cuts at: 6245 6446 8013
Size: 201 1567
Fragments arranged by size:
1870 1567 1081 741 708 693 524 406
222 201
BmgBI CAC'GTC
Cuts at: 0 642 1737 3255 3501 7419 8013
Size: 642 1095 1518 246 3918 594
Fragments arranged by size:
3918 1518 1095 642 594 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 308 2616 3634 3729 5558 5595 8013
Size: 308 2308 1018 95 1829 37 2418
Fragments arranged by size:
2418 2308 1829 1018 308 95 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 5142 8013
Size: 103 5039 2871
Fragments arranged by size:
5039 2871 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1901 2443 2584 3665 4142 4177 4537
Size: 472 1429 542 141 1081 477 35 360
Cuts at: 4537 4718 4749 6073 6142 6412 6490 6594 6646
Size: 181 31 1324 69 270 78 104 52
Cuts at: 6646 8013
Size: 1367
Fragments arranged by size:
1429 1367 1324 1081 542 477 472 360
270 181 141 104 78 69 52 35
31
Bpu10I CC'TnA_GC
Cuts at: 0 233 1517 1720 3046 4586 4684 5139 5162
Size: 233 1284 203 1326 1540 98 455 23
Cuts at: 5162 6830 7004 7335 7540 8013
Size: 1668 174 331 205 473
Fragments arranged by size:
1668 1540 1326 1284 473 455 331 233
205 203 174 98 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1951 2722 4488 5923 7843 8013
Size: 1951 771 1766 1435 1920 170
Fragments arranged by size:
1951 1920 1766 1435 771 170
BsaI GGTCTCn'nnnn_
Cuts at: 0 5924 6585 8013
Size: 5924 661 1428
Fragments arranged by size:
5924 1428 661
BsaAI yAC'GTr
Cuts at: 0 1116 1911 2766 4377 5810 8013
Size: 1116 795 855 1611 1433 2203
Fragments arranged by size:
2203 1611 1433 1116 855 795
BsaBI GATnn'nnATC
Cuts at: 0 2083 6454 6934 8013
Size: 2083 4371 480 1079
Fragments arranged by size:
4371 2083 1079 480
BsaHI Gr'CG_yC
Cuts at: 0 4975 5707 6890 8013
Size: 4975 732 1183 1123
Fragments arranged by size:
4975 1183 1123 732
BsaWI w'CCGG_w
Cuts at: 0 1951 2531 3422 3815 6446 7268 8013
Size: 1951 580 891 393 2631 822 745
Fragments arranged by size:
2631 1951 891 822 745 580 393
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1092 1122 1924 1954 2149 2179 2986 3016
Size: 1092 30 802 30 195 30 807 30
Cuts at: 3016 3466 3496 3715 3745 4513 4543 4912 4942
Size: 450 30 219 30 768 30 369 30
Cuts at: 4942 5840 5870 8013
Size: 898 30 2143
Fragments arranged by size:
2143 1092 898 807 802 768 450 369
219 195 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 828 1831 2296 2449 2479 3004 3007 3010
Size: 828 1003 465 153 30 525 3 3
Cuts at: 3010 3013 3259 3427 3430 3668 3913 4534 6070
Size: 3 246 168 3 238 245 621 1536
Cuts at: 6070 6178 6643 6856 7323 7393 7846 8013
Size: 108 465 213 467 70 453 167
Fragments arranged by size:
1536 1003 828 621 525 467 465 465
453 246 245 238 213 168 167 153
108 70 30 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 239 459 597 2308 2345 2507 3041 4055
Size: 239 220 138 1711 37 162 534 1014
Cuts at: 4055 4344 4543 4966 5453 5687 5790 6339 6635
Size: 289 199 423 487 234 103 549 296
Cuts at: 6635 7037 7302 8013
Size: 402 265 711
Fragments arranged by size:
1711 1014 711 549 534 487 423 402
296 289 265 239 234 220 199 162
138 103 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 603 631 1005 2065 2243 2269 2491
Size: 454 149 28 374 1060 178 26 222
Cuts at: 2491 2845 4320 5132 5834 6688 8013
Size: 354 1475 812 702 854 1325
Fragments arranged by size:
1475 1325 1060 854 812 702 454 374
354 222 178 149 28 26
BsiEI CG_ry'CG
Cuts at: 0 3634 8013
Size: 3634 4379
BsiHKAI G_wGCw'C
Cuts at: 0 1718 3308 4476 4496 4584 5128 8013
Size: 1718 1590 1168 20 88 544 2885
Fragments arranged by size:
2885 1718 1590 1168 544 88 20
BsmI GAATG_Cn'
Cuts at: 0 2178 6096 8013
Size: 2178 3918 1917
Fragments arranged by size:
3918 2178 1917
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7287 8013
Size: 7287 726
Bsp1286I G_dGCh'C
Cuts at: 0 149 575 741 1265 1704 1718 1967 2185
Size: 149 426 166 524 439 14 249 218
Cuts at: 2185 2346 2752 3154 3308 3973 4314 4476 4496
Size: 161 406 402 154 665 341 162 20
Cuts at: 4496 4584 4622 5128 5330 5786 6023 6245 6446
Size: 88 38 506 202 456 237 222 201
Cuts at: 6446 8013
Size: 1567
Fragments arranged by size:
1567 665 524 506 456 439 426 406
402 341 249 237 222 218 202 201
166 162 161 154 149 88 38 20
14
BspEI T'CCGG_A
Cuts at: 0 2531 3422 3815 7268 8013
Size: 2531 891 393 3453 745
Fragments arranged by size:
3453 2531 891 745 393
BspHI T'CATG_A
Cuts at: 0 1194 3510 5969 7604 8013
Size: 1194 2316 2459 1635 409
Fragments arranged by size:
2459 2316 1635 1194 409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 6 295 367 1346 1557 2517 3473 4050
Size: 6 289 72 979 211 960 956 577
Cuts at: 4050 4970 5294 6575 7980 8013
Size: 920 324 1281 1405 33
Fragments arranged by size:
1405 1281 979 960 956 920 577 324
289 211 72 33 6
BsrBI CCG'CTC
Cuts at: 0 5640 5678 6542 7580 8013
Size: 5640 38 864 1038 433
Fragments arranged by size:
5640 1038 864 433 38
BsrDI GCAATG_nn'
Cuts at: 0 4574 4727 5240 7568 8013
Size: 4574 153 513 2328 445
Fragments arranged by size:
4574 2328 513 445 153
BsrFI r'CCGG_y
Cuts at: 0 3629 4298 5173 7504 7641 8013
Size: 3629 669 875 2331 137 372
Fragments arranged by size:
3629 2331 875 669 372 137
BsrGI T'GTAC_A
Cuts at: 0 5666 8013
Size: 5666 2347
BssHII G'CGCG_C
Cuts at: 0 5394 8013
Size: 5394 2619
BssSI C'ACGA_G
Cuts at: 0 621 4096 6016 8013
Size: 621 3475 1920 1997
Fragments arranged by size:
3475 1997 1920 621
BstAPI GCAn_nnn'nTGC
Cuts at: 0 441 2071 4152 4794 7407 8013
Size: 441 1630 2081 642 2613 606
Fragments arranged by size:
2613 2081 1630 642 606 441
BstEII G'GTnAC_C
Cuts at: 0 913 8013
Size: 913 7100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2115 2886 3689 3987 4669 4915 5235 6618
Size: 2115 771 803 298 682 246 320 1383
Cuts at: 6618 7905 8013
Size: 1287 108
Fragments arranged by size:
2115 1383 1287 803 771 682 320 298
246 108
BstYI r'GATC_y
Cuts at: 0 120 777 3023 3419 4718 4758 5520 5762
Size: 120 657 2246 396 1299 40 762 242
Cuts at: 5762 5850 6462 7562 7901 8013
Size: 88 612 1100 339 112
Fragments arranged by size:
2246 1299 1100 762 657 612 396 339
242 120 112 88 40
Bsu36I CC'TnA_GG
Cuts at: 0 5012 5914 6488 6858 6895 7222 8013
Size: 5012 902 574 370 37 327 791
Fragments arranged by size:
5012 902 791 574 370 327 37
BtgI C'CryG_G
Cuts at: 0 482 887 1001 1918 2177 4883 5048 5092
Size: 482 405 114 917 259 2706 165 44
Cuts at: 5092 6023 6905 7181 7469 7550 7817 7982 8013
Size: 931 882 276 288 81 267 165 31
Fragments arranged by size:
2706 931 917 882 482 405 288 276
267 259 165 165 114 81 44 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4017 4976 5421 5945 8013
Size: 4017 959 445 524 2068
Fragments arranged by size:
4017 2068 959 524 445
BtsI GCAGTG_nn'
Cuts at: 0 143 251 330 442 961 993 1348 2237
Size: 143 108 79 112 519 32 355 889
Cuts at: 2237 2260 3216 3563 5621 7408 7524 7551 7741
Size: 23 956 347 2058 1787 116 27 190
Cuts at: 7741 8013
Size: 272
Fragments arranged by size:
2058 1787 956 889 519 355 347 272
190 143 116 112 108 79 32 27
23
ClaI AT'CG_AT
Cuts at: 0 177 2087 8013
Size: 177 1910 5926
Fragments arranged by size:
5926 1910 177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5760 5795 8013
Size: 5760 35 2218
Fragments arranged by size:
5760 2218 35
DraIII CAC_nnn'GTG
Cuts at: 0 483 1119 1848 3197 4482 8013
Size: 483 636 729 1349 1285 3531
Fragments arranged by size:
3531 1349 1285 729 636 483
DrdI GACnn_nn'nnGTC
Cuts at: 0 911 1133 4651 7195 8013
Size: 911 222 3518 2544 818
Fragments arranged by size:
3518 2544 911 818 222
EaeI y'GGCC_r
Cuts at: 0 998 2059 2597 3113 3631 3977 4724 5171
Size: 998 1061 538 516 518 346 747 447
Cuts at: 5171 5801 6108 6398 7643 7913 7964 8013
Size: 630 307 290 1245 270 51 49
Fragments arranged by size:
1245 1061 998 747 630 538 518 516
447 346 307 290 270 51 49
EagI C'GGCC_G
Cuts at: 0 3631 8013
Size: 3631 4382
EarI CTCTTCn'nnn_
Cuts at: 0 999 2816 2836 5920 6604 6692 7156 7370
Size: 999 1817 20 3084 684 88 464 214
Cuts at: 7370 7793 7871 8013
Size: 423 78 142
Fragments arranged by size:
3084 1817 999 684 464 423 214 142
88 78 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 3598 4285 4548 5658 6583 6862 7429
Size: 22 3576 687 263 1110 925 279 567
Cuts at: 7429 7467 7570 7643 8013
Size: 38 103 73 370
Fragments arranged by size:
3576 1110 925 687 567 370 279 263
103 73 38 22
EcoICRI GAG'CTC
Cuts at: 0 5126 8013
Size: 5126 2887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 858 1165 1586 1843 1901 2114 2443
Size: 472 386 307 421 257 58 213 329
Cuts at: 2443 2449 2584 2683 2803 2974 3004 3161 3665
Size: 6 135 99 120 171 30 157 504
Cuts at: 3665 3928 4142 4177 4258 4537 4718 4749 6073
Size: 263 214 35 81 279 181 31 1324
Cuts at: 6073 6142 6214 6379 6412 6490 6594 6646 7042
Size: 69 72 165 33 78 104 52 396
Cuts at: 7042 7114 7394 7801 7975 8013
Size: 72 280 407 174 38
Fragments arranged by size:
1324 504 472 421 407 396 386 329
307 280 279 263 257 214 213 181
174 171 165 157 135 120 104 99
81 78 72 72 69 58 52 38
35 33 31 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 198 810 1183 1930 4213 4519 8013
Size: 198 612 373 747 2283 306 3494
Fragments arranged by size:
3494 2283 747 612 373 306 198
EcoO109I rG'GnC_Cy
Cuts at: 0 203 1140 1202 1383 1923 2211 3050 4218
Size: 203 937 62 181 540 288 839 1168
Cuts at: 4218 5037 5326 6374 6899 7188 7218 7737 7826
Size: 819 289 1048 525 289 30 519 89
Cuts at: 7826 7949 8013
Size: 123 64
Fragments arranged by size:
1168 1048 937 839 819 540 525 519
289 289 288 203 181 123 89 64
62 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2715 3783 4308 5011 6053 7399 8013
Size: 2715 1068 525 703 1042 1346 614
Fragments arranged by size:
2715 1346 1068 1042 703 614 525
FspI TGC'GCA
Cuts at: 0 373 8013
Size: 373 7640
FspAI rTGC'GCAy
Cuts at: 0 373 8013
Size: 373 7640
HaeII r_GCGC'y
Cuts at: 0 665 2575 4684 5147 8013
Size: 665 1910 2109 463 2866
Fragments arranged by size:
2866 2109 1910 665 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 178 191 210 1092 1124 3466 3498
Size: 159 19 13 19 882 32 2342 32
Cuts at: 3498 3957 3989 4115 4147 4510 4542 4816 4848
Size: 459 32 126 32 363 32 274 32
Cuts at: 4848 6154 6186 6749 6781 7601 7633 8013
Size: 1306 32 563 32 820 32 380
Fragments arranged by size:
2342 1306 882 820 563 459 380 363
274 159 126 32 32 32 32 32
32 32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 0 2643 3876 6267 7851 7941 8013
Size: 2643 1233 2391 1584 90 72
Fragments arranged by size:
2643 2391 1584 1233 90 72
HindIII A'AGCT_T
Cuts at: 0 1292 2390 4624 6367 8013
Size: 1292 1098 2234 1743 1646
Fragments arranged by size:
2234 1743 1646 1292 1098
HpaI GTT'AAC
Cuts at: 0 7941 8013
Size: 7941 72
MfeI C'AATT_G
Cuts at: 0 6402 7844 8013
Size: 6402 1442 169
Fragments arranged by size:
6402 1442 169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 950 3809 5174 5630 7100 8013
Size: 707 243 2859 1365 456 1470 913
Fragments arranged by size:
2859 1470 1365 913 707 456 243
MscI TGG'CCA
Cuts at: 0 2061 2599 3115 3979 4726 5803 6400 7915
Size: 2061 538 516 864 747 1077 597 1515
Cuts at: 7915 7966 8013
Size: 51 47
Fragments arranged by size:
2061 1515 1077 864 747 597 538 516
51 47
MslI CAynn'nnrTG
Cuts at: 0 694 981 2755 2984 3809 5272 6274 7138
Size: 694 287 1774 229 825 1463 1002 864
Cuts at: 7138 7573 8013
Size: 435 440
Fragments arranged by size:
1774 1463 1002 864 825 694 440 435
287 229
MspA1I CmG'CkG
Cuts at: 0 2475 3045 3573 4082 4152 4593 5406 5691
Size: 2475 570 528 509 70 441 813 285
Cuts at: 5691 6093 6153 6343 6390 6956 7041 8013
Size: 402 60 190 47 566 85 972
Fragments arranged by size:
2475 972 813 570 566 528 509 441
402 285 190 85 70 60 47
NaeI GCC'GGC
Cuts at: 0 4300 7506 7643 8013
Size: 4300 3206 137 370
Fragments arranged by size:
4300 3206 370 137
NcoI C'CATG_G
Cuts at: 0 887 1001 2177 5048 7181 8013
Size: 887 114 1176 2871 2133 832
Fragments arranged by size:
2871 2133 1176 887 832 114
NgoMIV G'CCGG_C
Cuts at: 0 4298 7504 7641 8013
Size: 4298 3206 137 372
Fragments arranged by size:
4298 3206 372 137
NspI r_CATG'y
Cuts at: 0 597 1333 1350 3828 4926 5719 6751 7302
Size: 597 736 17 2478 1098 793 1032 551
Cuts at: 7302 8013
Size: 711
Fragments arranged by size:
2478 1098 1032 793 736 711 597 551
17
PasI CC'CwG_GG
Cuts at: 0 5628 8013
Size: 5628 2385
PciI A'CATG_T
Cuts at: 0 4922 8013
Size: 4922 3091
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 1416 3558 3741 5694 6120 8013
Size: 228 1188 2142 183 1953 426 1893
Fragments arranged by size:
2142 1953 1893 1188 426 228 183
PfoI T'CCnGG_A
Cuts at: 0 2798 4064 4742 4765 4871 7904 8013
Size: 2798 1266 678 23 106 3033 109
Fragments arranged by size:
3033 2798 1266 678 109 106 23
PmlI CAC'GTG
Cuts at: 0 1116 2766 4377 5810 8013
Size: 1116 1650 1611 1433 2203
Fragments arranged by size:
2203 1650 1611 1433 1116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3862 3894 8013
Size: 3862 32 4119
Fragments arranged by size:
4119 3862 32
PpuMI rG'GwC_Cy
Cuts at: 0 1140 1202 1923 2211 6374 7188 7737 7949
Size: 1140 62 721 288 4163 814 549 212
Cuts at: 7949 8013
Size: 64
Fragments arranged by size:
4163 1140 814 721 549 288 212 64
62
PspOMI G'GGCC_C
Cuts at: 0 5326 8013
Size: 5326 2687
PstI C_TGCA'G
Cuts at: 0 281 1343 1931 2504 2546 3470 4437 7046
Size: 281 1062 588 573 42 924 967 2609
Cuts at: 7046 7070 7217 7679 8013
Size: 24 147 462 334
Fragments arranged by size:
2609 1062 967 924 588 573 462 334
281 147 42 24
PvuII CAG'CTG
Cuts at: 0 2475 3045 4082 4152 4593 5691 6093 6153
Size: 2475 570 1037 70 441 1098 402 60
Cuts at: 6153 6390 6956 7041 8013
Size: 237 566 85 972
Fragments arranged by size:
2475 1098 1037 972 570 566 441 402
237 85 70 60
RsrII CG'GwC_CG
Cuts at: 0 2205 5021 8013
Size: 2205 2816 2992
Fragments arranged by size:
2992 2816 2205
SacI G_AGCT'C
Cuts at: 0 5128 8013
Size: 5128 2885
SalI G'TCGA_C
Cuts at: 0 2641 8013
Size: 2641 5372
SanDI GG'GwC_CC
Cuts at: 0 6374 8013
Size: 6374 1639
SapI GCTCTTCn'nnn_
Cuts at: 0 2816 5920 6692 7793 8013
Size: 2816 3104 772 1101 220
Fragments arranged by size:
3104 2816 1101 772 220
SbfI CC_TGCA'GG
Cuts at: 0 1931 7070 8013
Size: 1931 5139 943
Fragments arranged by size:
5139 1931 943
ScaI AGT'ACT
Cuts at: 0 547 8013
Size: 547 7466
SfcI C'TryA_G
Cuts at: 0 277 1339 1927 2500 2542 3466 4433 5222
Size: 277 1062 588 573 42 924 967 789
Cuts at: 5222 7042 7066 7213 7396 7675 7830 8013
Size: 1820 24 147 183 279 155 183
Fragments arranged by size:
1820 1062 967 924 789 588 573 279
277 183 183 155 147 42 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4205 5044 7972 8013
Size: 4205 839 2928 41
Fragments arranged by size:
4205 2928 839 41
SgrAI Cr'CCGG_yG
Cuts at: 0 4298 8013
Size: 4298 3715
SmaI CCC'GGG
Cuts at: 0 531 3072 6274 8013
Size: 531 2541 3202 1739
Fragments arranged by size:
3202 2541 1739 531
SmlI C'TyrA_G
Cuts at: 0 1966 2737 4467 5902 6979 7858 8013
Size: 1966 771 1730 1435 1077 879 155
Fragments arranged by size:
1966 1730 1435 1077 879 771 155
SphI G_CATG'C
Cuts at: 0 597 1333 7302 8013
Size: 597 736 5969 711
Fragments arranged by size:
5969 736 711 597
StuI AGG'CCT
Cuts at: 0 1906 5468 5912 8013
Size: 1906 3562 444 2101
Fragments arranged by size:
3562 2101 1906 444
StyI C'CwwG_G
Cuts at: 0 887 1001 1205 2177 2708 4448 5040 5048
Size: 887 114 204 972 531 1740 592 8
Cuts at: 5048 5514 6182 7181 7347 7802 8013
Size: 466 668 999 166 455 211
Fragments arranged by size:
1740 999 972 887 668 592 531 466
455 211 204 166 114 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 2042 2881 3126 3381 3815 3821 4657
Size: 451 1591 839 245 255 434 6 836
Cuts at: 4657 5701 8013
Size: 1044 2312
Fragments arranged by size:
2312 1591 1044 839 836 451 434 255
245 6
TatI w'GTAC_w
Cuts at: 0 545 1618 2624 4925 5666 8013
Size: 545 1073 1006 2301 741 2347
Fragments arranged by size:
2347 2301 1073 1006 741 545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 421 453 907 939 1431 1463 1600 1632
Size: 421 32 454 32 492 32 137 32
Cuts at: 1632 1924 1956 2014 2046 2133 2165 2986 3018
Size: 292 32 58 32 87 32 821 32
Cuts at: 3018 3685 3717 4513 4545 5237 5269 5840 5872
Size: 667 32 796 32 692 32 571 32
Cuts at: 5872 8013
Size: 2141
Fragments arranged by size:
2141 821 796 692 667 571 492 454
421 292 137 87 58 32 32 32
32 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 643 2209 8013
Size: 643 1566 5804
Fragments arranged by size:
5804 1566 643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 586 2175 3705 5257 7989 8013
Size: 225 361 1589 1530 1552 2732 24
Fragments arranged by size:
2732 1589 1552 1530 361 225 24
XhoI C'TCGA_G
Cuts at: 0 6979 8013
Size: 6979 1034
XmaI C'CCGG_G
Cuts at: 0 529 3070 6272 8013
Size: 529 2541 3202 1741
Fragments arranged by size:
3202 2541 1741 529
XmnI GAAnn'nnTTC
Cuts at: 0 2698 4291 7027 8013
Size: 2698 1593 2736 986
Fragments arranged by size:
2736 2698 1593 986
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI BaeI BbeI BfrBI BmtI
BsiWI BstBI BstZ17I DraI EcoRI EcoRV FseI KasI
KpnI MluI NarI NdeI NheI NotI NruI NsiI
PacI PmeI PshAI PsiI PspXI PsrI PvuI SacII
SexAI SfoI SgfI SnaBI SpeI SrfI SspI SwaI
XbaI ZraI