Restriction map for
pF1KSDA0221
(Linear) (Six-base) MAPSORT of: KIAA0221.seq Check: 2298 from: 1 to: 3387
KIAA0221.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2702 3387
Size: 2702 685
AatII G_ACGT'C
Cuts at: 0 1537 3387
Size: 1537 1850
AccI GT'mk_AC
Cuts at: 0 1282 1550 3387
Size: 1282 268 1837
Fragments arranged by size:
1837 1282 268
Acc65I G'GTAC_C
Cuts at: 0 1814 3387
Size: 1814 1573
AclI AA'CG_TT
Cuts at: 0 1318 3387
Size: 1318 2069
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 2395 3387
Size: 979 1416 992
Fragments arranged by size:
1416 992 979
AfeI AGC'GCT
Cuts at: 0 1417 2431 3387
Size: 1417 1014 956
Fragments arranged by size:
1417 1014 956
AflIII A'CryG_T
Cuts at: 0 1879 3387
Size: 1879 1508
AleI CACnn'nnGTG
Cuts at: 0 1882 3387
Size: 1882 1505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 653 3387
Size: 653 2734
AlwNI CAG_nnn'CTG
Cuts at: 0 374 693 1761 1770 3387
Size: 374 319 1068 9 1617
Fragments arranged by size:
1617 1068 374 319 9
ApaI G_GGCC'C
Cuts at: 0 23 239 524 923 1510 2035 3387
Size: 23 216 285 399 587 525 1352
Fragments arranged by size:
1352 587 525 399 285 216 23
ApaLI G'TGCA_C
Cuts at: 0 1232 3387
Size: 1232 2155
AvaI C'yCGr_G
Cuts at: 0 159 183 1899 1987 2310 2805 2843 3387
Size: 159 24 1716 88 323 495 38 544
Fragments arranged by size:
1716 544 495 323 159 88 38 24
BanI G'GyrC_C
Cuts at: 0 73 1814 1888 2314 2383 3058 3187 3387
Size: 73 1741 74 426 69 675 129 200
Fragments arranged by size:
1741 675 426 200 129 74 73 69
BanII G_rGCy'C
Cuts at: 0 23 91 205 239 524 923 1510 2035
Size: 23 68 114 34 285 399 587 525
Cuts at: 2035 2191 2814 3387
Size: 156 623 573
Fragments arranged by size:
623 587 573 525 399 285 156 114
68 34 23
BbeI G_GCGC'C
Cuts at: 0 77 1892 2387 3191 3387
Size: 77 1815 495 804 196
Fragments arranged by size:
1815 804 495 196 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 564 1477 1531 3387
Size: 335 229 913 54 1856
Fragments arranged by size:
1856 913 335 229 54
BclI T'GATC_A
Cuts at: 0 2936 3387
Size: 2936 451
BglI GCCn_nnn'nGGC
Cuts at: 0 155 188 1514 2030 2848 3242 3387
Size: 155 33 1326 516 818 394 145
Fragments arranged by size:
1326 818 516 394 155 145 33
Bme1580I G_kGCm'C
Cuts at: 0 23 239 524 762 923 1236 1510 2035
Size: 23 216 285 238 161 313 274 525
Cuts at: 2035 2317 3387
Size: 282 1070
Fragments arranged by size:
1070 525 313 285 282 274 238 216
161 23
BmgBI CAC'GTC
Cuts at: 0 442 1170 3309 3387
Size: 442 728 2139 78
Fragments arranged by size:
2139 728 442 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 1793 2029 3375 3387
Size: 678 1115 236 1346 12
Fragments arranged by size:
1346 1115 678 236 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 814 850 859 955 2179 3387
Size: 562 252 36 9 96 1224 1208
Fragments arranged by size:
1224 1208 562 252 96 36 9
Bpu10I CC'TnA_GC
Cuts at: 0 1748 3387
Size: 1748 1639
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 959 3387
Size: 640 319 2428
Fragments arranged by size:
2428 640 319
BsaI GGTCTCn'nnnn_
Cuts at: 0 1327 3387
Size: 1327 2060
BsaAI yAC'GTr
Cuts at: 0 2280 2958 3332 3387
Size: 2280 678 374 55
Fragments arranged by size:
2280 678 374 55
BsaBI GATnn'nnATC
Cuts at: 0 2404 3387
Size: 2404 983
BsaHI Gr'CG_yC
Cuts at: 0 74 136 893 1534 1889 2384 2513 2604
Size: 74 62 757 641 355 495 129 91
Cuts at: 2604 2639 3090 3166 3188 3387
Size: 35 451 76 22 199
Fragments arranged by size:
757 641 495 451 355 199 129 91
76 74 62 35 22
BsaWI w'CCGG_w
Cuts at: 0 2138 3387
Size: 2138 1249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 787 817 1780 1810 2173 2203 3387
Size: 787 30 963 30 363 30 1184
Fragments arranged by size:
1184 963 787 363 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 568 811 874 2311 3387
Size: 70 498 243 63 1437 1076
Fragments arranged by size:
1437 1076 498 243 70 63
BseYI C'CCAG_C
Cuts at: 0 22 2028 2103 3121 3251 3387
Size: 22 2006 75 1018 130 136
Fragments arranged by size:
2006 1018 136 130 75 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 645 1699 1860 2135 2455 2464 3387
Size: 493 152 1054 161 275 320 9 923
Fragments arranged by size:
1054 923 493 320 275 161 152 9
BsiHKAI G_wGCw'C
Cuts at: 0 1236 1596 2573 3387
Size: 1236 360 977 814
Fragments arranged by size:
1236 977 814 360
BsiWI C'GTAC_G
Cuts at: 0 14 3387
Size: 14 3373
Bsp1286I G_dGCh'C
Cuts at: 0 23 91 205 239 524 762 923 1236
Size: 23 68 114 34 285 238 161 313
Cuts at: 1236 1510 1596 2035 2191 2317 2573 2814 3387
Size: 274 86 439 156 126 256 241 573
Fragments arranged by size:
573 439 313 285 274 256 241 238
161 156 126 114 86 68 34 23
BspHI T'CATG_A
Cuts at: 0 2819 3387
Size: 2819 568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 2118 2702 2756 3387
Size: 219 1899 584 54 631
Fragments arranged by size:
1899 631 584 219 54
BsrBI CCG'CTC
Cuts at: 0 1925 2682 2739 3345 3387
Size: 1925 757 57 606 42
Fragments arranged by size:
1925 757 606 57 42
BsrFI r'CCGG_y
Cuts at: 0 1578 1891 2891 2944 3387
Size: 1578 313 1000 53 443
Fragments arranged by size:
1578 1000 443 313 53
BssSI C'ACGA_G
Cuts at: 0 1375 3387
Size: 1375 2012
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1761 2451 2721 3387
Size: 1761 690 270 666
Fragments arranged by size:
1761 690 666 270
BstEII G'GTnAC_C
Cuts at: 0 501 1894 3387
Size: 501 1393 1493
Fragments arranged by size:
1493 1393 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2581 3387
Size: 2581 806
BstYI r'GATC_y
Cuts at: 0 731 1631 3387
Size: 731 900 1756
Fragments arranged by size:
1756 900 731
BtgI C'CryG_G
Cuts at: 0 1325 1610 1979 2779 3387
Size: 1325 285 369 800 608
Fragments arranged by size:
1325 800 608 369 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1211 1590 3387
Size: 1211 379 1797
Fragments arranged by size:
1797 1211 379
BtsI GCAGTG_nn'
Cuts at: 0 688 2019 2207 3387
Size: 688 1331 188 1180
Fragments arranged by size:
1331 1180 688 188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 423 458 3387
Size: 423 35 2929
Fragments arranged by size:
2929 423 35
DraI TTT'AAA
Cuts at: 0 2591 3294 3387
Size: 2591 703 93
Fragments arranged by size:
2591 703 93
DraIII CAC_nnn'GTG
Cuts at: 0 480 1380 3387
Size: 480 900 2007
Fragments arranged by size:
2007 900 480
DrdI GACnn_nn'nnGTC
Cuts at: 0 1341 1533 3375 3387
Size: 1341 192 1842 12
Fragments arranged by size:
1842 1341 192 12
EaeI y'GGCC_r
Cuts at: 0 593 1164 1538 1907 2057 3387
Size: 593 571 374 369 150 1330
Fragments arranged by size:
1330 593 571 374 369 150
EarI CTCTTCn'nnn_
Cuts at: 0 122 1012 2089 3387
Size: 122 890 1077 1298
Fragments arranged by size:
1298 1077 890 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2110 3387
Size: 2110 1277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 814 850 859 955 979 2179 2395
Size: 562 252 36 9 96 24 1200 216
Cuts at: 2395 3387
Size: 992
Fragments arranged by size:
1200 992 562 252 216 96 36 24
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 511 1293 1501 3047 3196 3387
Size: 511 782 208 1546 149 191
Fragments arranged by size:
1546 782 511 208 191 149
EcoO109I rG'GnC_Cy
Cuts at: 0 348 521 834 920 999 1137 1179 1431
Size: 348 173 313 86 79 138 42 252
Cuts at: 1431 1506 3387
Size: 75 1881
Fragments arranged by size:
1881 348 313 252 173 138 86 79
75 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1156 3120 3387
Size: 1156 1964 267
Fragments arranged by size:
1964 1156 267
FseI GG_CCGG'CC
Cuts at: 0 2895 3387
Size: 2895 492
HaeII r_GCGC'y
Cuts at: 0 77 1419 1892 2094 2162 2387 2433 3191
Size: 77 1342 473 202 68 225 46 758
Cuts at: 3191 3316 3387
Size: 125 71
Fragments arranged by size:
1342 758 473 225 202 125 77 71
68 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 1780 1812 3151 3183 3280 3312
Size: 1321 32 427 32 1339 32 97 32
Cuts at: 3312 3387
Size: 75
Fragments arranged by size:
1339 1321 427 97 75 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1283 2793 3387
Size: 1283 1510 594
Fragments arranged by size:
1510 1283 594
KasI G'GCGC_C
Cuts at: 0 73 1888 2383 3187 3387
Size: 73 1815 495 804 200
Fragments arranged by size:
1815 804 495 200 73
KpnI G_GTAC'C
Cuts at: 0 1818 3387
Size: 1818 1569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2195 3387
Size: 2195 1192
MscI TGG'CCA
Cuts at: 0 1909 3387
Size: 1909 1478
MslI CAynn'nnrTG
Cuts at: 0 1609 1882 2647 2830 3211 3387
Size: 1609 273 765 183 381 176
Fragments arranged by size:
1609 765 381 273 183 176
MspA1I CmG'CkG
Cuts at: 0 693 707 753 1407 1770 2001 2028 2215
Size: 693 14 46 654 363 231 27 187
Cuts at: 2215 2462 3387
Size: 247 925
Fragments arranged by size:
925 693 654 363 247 231 187 46
27 14
NaeI GCC'GGC
Cuts at: 0 2893 2946 3387
Size: 2893 53 441
Fragments arranged by size:
2893 441 53
NarI GG'CG_CC
Cuts at: 0 74 1889 2384 3188 3387
Size: 74 1815 495 804 199
Fragments arranged by size:
1815 804 495 199 74
NgoMIV G'CCGG_C
Cuts at: 0 2891 2944 3387
Size: 2891 53 443
Fragments arranged by size:
2891 443 53
NspI r_CATG'y
Cuts at: 0 874 1095 1746 1883 2454 2907 3387
Size: 874 221 651 137 571 453 480
Fragments arranged by size:
874 651 571 480 453 221 137
PasI CC'CwG_GG
Cuts at: 0 525 2286 3387
Size: 525 1761 1101
Fragments arranged by size:
1761 1101 525
PciI A'CATG_T
Cuts at: 0 1879 3387
Size: 1879 1508
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 1380 2493 3016 3162 3387
Size: 297 1083 1113 523 146 225
Fragments arranged by size:
1113 1083 523 297 225 146
PfoI T'CCnGG_A
Cuts at: 0 44 539 698 3114 3387
Size: 44 495 159 2416 273
Fragments arranged by size:
2416 495 273 159 44
PmlI CAC'GTG
Cuts at: 0 2958 3387
Size: 2958 429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 3387
Size: 85 32 3270
Fragments arranged by size:
3270 85 32
PpuMI rG'GwC_Cy
Cuts at: 0 348 834 999 1137 1179 3387
Size: 348 486 165 138 42 2208
Fragments arranged by size:
2208 486 348 165 138 42
PspOMI G'GGCC_C
Cuts at: 0 19 235 520 919 1506 2031 3387
Size: 19 216 285 399 587 525 1356
Fragments arranged by size:
1356 587 525 399 285 216 19
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2440 2472 3387
Size: 2440 32 915
Fragments arranged by size:
2440 915 32
PstI C_TGCA'G
Cuts at: 0 254 370 1757 1766 2129 2214 3051 3387
Size: 254 116 1387 9 363 85 837 336
Fragments arranged by size:
1387 837 363 336 254 116 85 9
PvuII CAG'CTG
Cuts at: 0 753 1407 1770 2001 2028 3387
Size: 753 654 363 231 27 1359
Fragments arranged by size:
1359 753 654 363 231 27
SalI G'TCGA_C
Cuts at: 0 1281 3387
Size: 1281 2106
SapI GCTCTTCn'nnn_
Cuts at: 0 2089 3387
Size: 2089 1298
SbfI CC_TGCA'GG
Cuts at: 0 2129 3387
Size: 2129 1258
SexAI A'CCwGG_T
Cuts at: 0 2438 2990 3387
Size: 2438 552 397
Fragments arranged by size:
2438 552 397
SfcI C'TryA_G
Cuts at: 0 250 366 1753 1762 2125 2210 3047 3387
Size: 250 116 1387 9 363 85 837 340
Fragments arranged by size:
1387 837 363 340 250 116 85 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 155 3387
Size: 155 3232
SfoI GGC'GCC
Cuts at: 0 75 1890 2385 3189 3387
Size: 75 1815 495 804 198
Fragments arranged by size:
1815 804 495 198 75
SgrAI Cr'CCGG_yG
Cuts at: 0 1891 3387
Size: 1891 1496
SmaI CCC'GGG
Cuts at: 0 161 185 2807 2845 3387
Size: 161 24 2622 38 542
Fragments arranged by size:
2622 542 161 38 24
SmlI C'TyrA_G
Cuts at: 0 655 974 3387
Size: 655 319 2413
Fragments arranged by size:
2413 655 319
SphI G_CATG'C
Cuts at: 0 1746 3387
Size: 1746 1641
SrfI GCCC'GGGC
Cuts at: 0 161 185 3387
Size: 161 24 3202
Fragments arranged by size:
3202 161 24
StyI C'CwwG_G
Cuts at: 0 2060 2574 3279 3387
Size: 2060 514 705 108
Fragments arranged by size:
2060 705 514 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1219 1963 2350 3018 3387
Size: 1219 744 387 668 369
Fragments arranged by size:
1219 744 668 387 369
TatI w'GTAC_w
Cuts at: 0 2447 2906 3387
Size: 2447 459 481
Fragments arranged by size:
2447 481 459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 787 819 1258 1290 3387
Size: 787 32 439 32 2097
Fragments arranged by size:
2097 787 439 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 294 2926 3159 3387
Size: 294 2632 233 228
Fragments arranged by size:
2632 294 233 228
XmaI C'CCGG_G
Cuts at: 0 159 183 2805 2843 3387
Size: 159 24 2622 38 544
Fragments arranged by size:
2622 544 159 38 24
XmnI GAAnn'nnTTC
Cuts at: 0 427 450 913 2536 3387
Size: 427 23 463 1623 851
Fragments arranged by size:
1623 851 463 427 23
ZraI GAC'GTC
Cuts at: 0 1535 3387
Size: 1535 1852
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbsI BclI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I BspHI BspMI
BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuII SalI SapI
SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI SrfI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglII
BlpI BmtI BplI BsiEI BsmI BsmBI BspEI BsrDI
BsrGI BssHII BstBI BstZ17I Bsu36I ClaI EagI EcoICRI
EcoRI EcoRV FspI FspAI HindIII HpaI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PmeI
PshAI PsiI PspXI PvuI RsrII SacI SacII SanDI
ScaI SgfI SnaBI SpeI SspI StuI SwaI Tth111I
XbaI XhoI