Restriction map for
pF1KSDA0222
(Linear) (Six-base) MAPSORT of: KIAA0222.seq Check: 55 from: 1 to: 3489
KIAA0222.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 112 3489
Size: 112 3377
AccI GT'mk_AC
Cuts at: 0 2215 3489
Size: 2215 1274
Acc65I G'GTAC_C
Cuts at: 0 3454 3489
Size: 3454 35
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 753 808 2757 3416 3489
Size: 250 503 55 1949 659 73
Fragments arranged by size:
1949 659 503 250 73 55
AflIII A'CryG_T
Cuts at: 0 1602 3489
Size: 1602 1887
AgeI A'CCGG_T
Cuts at: 0 2745 3489
Size: 2745 744
AleI CACnn'nnGTG
Cuts at: 0 2515 3489
Size: 2515 974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 394 469 2533 3489
Size: 394 75 2064 956
Fragments arranged by size:
2064 956 394 75
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 850 882 3489
Size: 850 32 2607
Fragments arranged by size:
2607 850 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1158 1945 3027 3489
Size: 1158 787 1082 462
Fragments arranged by size:
1158 1082 787 462
ApaI G_GGCC'C
Cuts at: 0 686 1127 1427 1662 2452 2711 3345 3489
Size: 686 441 300 235 790 259 634 144
Fragments arranged by size:
790 686 634 441 300 259 235 144
ApaLI G'TGCA_C
Cuts at: 0 577 3489
Size: 577 2912
ApoI r'AATT_y
Cuts at: 0 2929 3489
Size: 2929 560
AvaI C'yCGr_G
Cuts at: 0 498 721 733 1740 2314 2757 2810 3386
Size: 498 223 12 1007 574 443 53 576
Cuts at: 3386 3489
Size: 103
Fragments arranged by size:
1007 576 574 498 443 223 103 53
12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3438 3471 3489
Size: 3438 33 18
Fragments arranged by size:
3438 33 18
BanI G'GyrC_C
Cuts at: 0 297 406 1789 2049 2345 2517 2634 2748
Size: 297 109 1383 260 296 172 117 114
Cuts at: 2748 3102 3454 3489
Size: 354 352 35
Fragments arranged by size:
1383 354 352 297 296 260 172 117
114 109 35
BanII G_rGCy'C
Cuts at: 0 142 686 733 817 863 1127 1427 1662
Size: 142 544 47 84 46 264 300 235
Cuts at: 1662 1747 1862 2452 2711 3005 3345 3489
Size: 85 115 590 259 294 340 144
Fragments arranged by size:
590 544 340 300 294 264 259 235
144 142 115 85 84 47 46
BbeI G_GCGC'C
Cuts at: 0 301 410 1793 2638 3106 3489
Size: 301 109 1383 845 468 383
Fragments arranged by size:
1383 845 468 383 301 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 1778 3489
Size: 1778 1711
BbvCI CC'TCA_GC
Cuts at: 0 31 3028 3489
Size: 31 2997 461
Fragments arranged by size:
2997 461 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1747 1781 3489
Size: 1747 34 1708
Fragments arranged by size:
1747 1708 34
BglI GCCn_nnn'nGGC
Cuts at: 0 397 433 1288 1762 3079 3489
Size: 397 36 855 474 1317 410
Fragments arranged by size:
1317 855 474 410 397 36
BglII A'GATC_T
Cuts at: 0 1565 3489
Size: 1565 1924
BlpI GC'TnA_GC
Cuts at: 0 646 727 1223 2939 3489
Size: 646 81 496 1716 550
Fragments arranged by size:
1716 646 550 496 81
Bme1580I G_kGCm'C
Cuts at: 0 581 686 1127 1195 1427 1662 2054 2452
Size: 581 105 441 68 232 235 392 398
Cuts at: 2452 2711 2910 3345 3357 3489
Size: 259 199 435 12 132
Fragments arranged by size:
581 441 435 398 392 259 235 232
199 132 105 68 12
BmgBI CAC'GTC
Cuts at: 0 1338 3489
Size: 1338 2151
BmtI G_CTAG'C
Cuts at: 0 2585 2738 3489
Size: 2585 153 751
Fragments arranged by size:
2585 751 153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 1704 2265 2754 3024 3280 3489
Size: 1143 561 561 489 270 256 209
Fragments arranged by size:
1143 561 561 489 270 256 209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1135 1848 3489
Size: 586 549 713 1641
Fragments arranged by size:
1641 713 586 549
Bpu10I CC'TnA_GC
Cuts at: 0 31 991 3028 3489
Size: 31 960 2037 461
Fragments arranged by size:
2037 960 461 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1285 3489
Size: 856 429 2204
Fragments arranged by size:
2204 856 429
BsaI GGTCTCn'nnnn_
Cuts at: 0 1865 3431 3489
Size: 1865 1566 58
Fragments arranged by size:
1865 1566 58
BsaAI yAC'GTr
Cuts at: 0 576 3489
Size: 576 2913
BsaBI GATnn'nnATC
Cuts at: 0 238 3489
Size: 238 3251
BsaHI Gr'CG_yC
Cuts at: 0 298 335 407 1790 2635 3103 3419 3489
Size: 298 37 72 1383 845 468 316 70
Fragments arranged by size:
1383 845 468 316 298 72 70 37
BsaWI w'CCGG_w
Cuts at: 0 242 2611 2745 3266 3489
Size: 242 2369 134 521 223
Fragments arranged by size:
2369 521 242 223 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 799 829 850 880 1678 1708
Size: 730 30 39 30 21 30 798 30
Cuts at: 1708 3278 3308 3489
Size: 1570 30 181
Fragments arranged by size:
1570 798 730 181 39 30 30 30
30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 985 1123 1798 2241 2972 2975 3489
Size: 649 336 138 675 443 731 3 514
Fragments arranged by size:
731 675 649 514 443 336 138 3
BseYI C'CCAG_C
Cuts at: 0 43 303 1697 1702 2230 2868 3083 3097
Size: 43 260 1394 5 528 638 215 14
Cuts at: 3097 3489
Size: 392
Fragments arranged by size:
1394 638 528 392 260 215 43 14
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 624 633 1579 1787 3489
Size: 556 68 9 946 208 1702
Fragments arranged by size:
1702 946 556 208 68 9
BsiEI CG_ry'CG
Cuts at: 0 63 846 1106 1181 1292 1493 1856 3489
Size: 63 783 260 75 111 201 363 1633
Fragments arranged by size:
1633 783 363 260 201 111 75 63
BsiHKAI G_wGCw'C
Cuts at: 0 142 529 581 1862 2940 3254 3489
Size: 142 387 52 1281 1078 314 235
Fragments arranged by size:
1281 1078 387 314 235 142 52
BsmI GAATG_Cn'
Cuts at: 0 2482 3489
Size: 2482 1007
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2236 3489
Size: 2236 1253
Bsp1286I G_dGCh'C
Cuts at: 0 142 529 581 686 733 817 863 1127
Size: 142 387 52 105 47 84 46 264
Cuts at: 1127 1195 1427 1662 1747 1862 2054 2452 2711
Size: 68 232 235 85 115 192 398 259
Cuts at: 2711 2910 2940 3005 3254 3345 3357 3489
Size: 199 30 65 249 91 12 132
Fragments arranged by size:
398 387 264 259 249 235 232 199
192 142 132 115 105 91 85 84
68 65 52 47 46 30 12
BspEI T'CCGG_A
Cuts at: 0 242 2611 3266 3489
Size: 242 2369 655 223
Fragments arranged by size:
2369 655 242 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 112 1809 3489
Size: 103 9 1697 1680
Fragments arranged by size:
1697 1680 103 9
BsrBI CCG'CTC
Cuts at: 0 1445 1487 2095 2438 3489
Size: 1445 42 608 343 1051
Fragments arranged by size:
1445 1051 608 343 42
BsrFI r'CCGG_y
Cuts at: 0 58 374 985 1180 1287 1468 1525 1915
Size: 58 316 611 195 107 181 57 390
Cuts at: 1915 2745 3489
Size: 830 744
Fragments arranged by size:
830 744 611 390 316 195 181 107
58 57
BssHII G'CGCG_C
Cuts at: 0 315 1095 1437 1495 1616 1618 2819 3489
Size: 315 780 342 58 121 2 1201 670
Fragments arranged by size:
1201 780 670 342 315 121 58 2
BssSI C'ACGA_G
Cuts at: 0 70 277 1188 3489
Size: 70 207 911 2301
Fragments arranged by size:
2301 911 207 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 611 2049 2477 3305 3489
Size: 156 455 1438 428 828 184
Fragments arranged by size:
1438 828 455 428 184 156
BstEII G'GTnAC_C
Cuts at: 0 2110 3489
Size: 2110 1379
BstYI r'GATC_y
Cuts at: 0 1565 3489
Size: 1565 1924
Bsu36I CC'TnA_GG
Cuts at: 0 1165 2945 3337 3489
Size: 1165 1780 392 152
Fragments arranged by size:
1780 1165 392 152
BtgI C'CryG_G
Cuts at: 0 825 1429 2366 2377 3489
Size: 825 604 937 11 1112
Fragments arranged by size:
1112 937 825 604 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 1594 2790 3489
Size: 927 667 1196 699
Fragments arranged by size:
1196 927 699 667
DraIII CAC_nnn'GTG
Cuts at: 0 75 1678 3363 3489
Size: 75 1603 1685 126
Fragments arranged by size:
1685 1603 126 75
EaeI y'GGCC_r
Cuts at: 0 60 759 843 1103 1178 1262 1280 1289
Size: 60 699 84 260 75 84 18 9
Cuts at: 1289 1328 1470 1490 1527 1754 2676 2696 2714
Size: 39 142 20 37 227 922 20 18
Cuts at: 2714 2831 3038 3140 3280 3330 3489
Size: 117 207 102 140 50 159
Fragments arranged by size:
922 699 260 227 207 159 142 140
117 102 84 84 75 60 50 39
37 20 20 18 18 9
EagI C'GGCC_G
Cuts at: 0 60 843 1103 1178 1289 1490 3489
Size: 60 783 260 75 111 201 1999
Fragments arranged by size:
1999 783 260 201 111 75 60
EarI CTCTTCn'nnn_
Cuts at: 0 1835 2556 3489
Size: 1835 721 933
Fragments arranged by size:
1835 933 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1132 1707 3489
Size: 1132 575 1782
Fragments arranged by size:
1782 1132 575
EcoICRI GAG'CTC
Cuts at: 0 140 1860 3489
Size: 140 1720 1629
Fragments arranged by size:
1720 1629 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 586 753 808 1135 1848 2757 3416
Size: 250 336 167 55 327 713 909 659
Cuts at: 3416 3489
Size: 73
Fragments arranged by size:
909 713 659 336 327 250 167 73
55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1000 1805 3217 3489
Size: 1000 805 1412 272
Fragments arranged by size:
1412 1000 805 272
EcoO109I rG'GnC_Cy
Cuts at: 0 40 327 389 414 425 561 682 1123
Size: 40 287 62 25 11 136 121 441
Cuts at: 1123 1124 1310 1423 1424 1455 2319 2449 2617
Size: 1 186 113 1 31 864 130 168
Cuts at: 2617 2707 2708 2862 3341 3489
Size: 90 1 154 479 148
Fragments arranged by size:
864 479 441 287 186 168 154 148
136 130 121 113 90 62 40 31
25 11 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1161 2567 3489
Size: 1161 1406 922
Fragments arranged by size:
1406 1161 922
FseI GG_CCGG'CC
Cuts at: 0 62 3489
Size: 62 3427
FspI TGC'GCA
Cuts at: 0 160 3489
Size: 160 3329
HaeII r_GCGC'y
Cuts at: 0 301 362 410 1327 1793 2176 2638 2786
Size: 301 61 48 917 466 383 462 148
Cuts at: 2786 2981 3106 3162 3489
Size: 195 125 56 327
Fragments arranged by size:
917 466 462 383 327 301 195 148
125 61 56 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1471 1503 1678 1710 1885 1917 3215 3247
Size: 1471 32 175 32 175 32 1298 32
Cuts at: 3247 3278 3310 3456 3488 3489
Size: 31 32 146 32 1
Fragments arranged by size:
1471 1298 175 175 146 32 32 32
32 32 32 31 1
HincII GTy'rAC
Cuts at: 0 1239 2216 3489
Size: 1239 977 1273
Fragments arranged by size:
1273 1239 977
KasI G'GCGC_C
Cuts at: 0 297 406 1789 2634 3102 3489
Size: 297 109 1383 845 468 387
Fragments arranged by size:
1383 845 468 387 297 109
KpnI G_GTAC'C
Cuts at: 0 3458 3489
Size: 3458 31
MluI A'CGCG_T
Cuts at: 0 1602 3489
Size: 1602 1887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3227 3489
Size: 3227 262
MscI TGG'CCA
Cuts at: 0 761 1264 2716 3040 3489
Size: 761 503 1452 324 449
Fragments arranged by size:
1452 761 503 449 324
MslI CAynn'nnrTG
Cuts at: 0 2515 3489
Size: 2515 974
MspA1I CmG'CkG
Cuts at: 0 303 443 517 692 1260 1431 1664 1729
Size: 303 140 74 175 568 171 233 65
Cuts at: 1729 1826 2357 2872 3236 3489
Size: 97 531 515 364 253
Fragments arranged by size:
568 531 515 364 303 253 233 175
171 140 97 74 65
NaeI GCC'GGC
Cuts at: 0 60 987 1182 1289 1470 3489
Size: 60 927 195 107 181 2019
Fragments arranged by size:
2019 927 195 181 107 60
NarI GG'CG_CC
Cuts at: 0 298 407 1790 2635 3103 3489
Size: 298 109 1383 845 468 386
Fragments arranged by size:
1383 845 468 386 298 109
NgoMIV G'CCGG_C
Cuts at: 0 58 985 1180 1287 1468 3489
Size: 58 927 195 107 181 2021
Fragments arranged by size:
2021 927 195 181 107 58
NheI G'CTAG_C
Cuts at: 0 2581 2734 3489
Size: 2581 153 755
Fragments arranged by size:
2581 755 153
NspI r_CATG'y
Cuts at: 0 159 494 2589 3351 3489
Size: 159 335 2095 762 138
Fragments arranged by size:
2095 762 335 159 138
PasI CC'CwG_GG
Cuts at: 0 2559 3241 3489
Size: 2559 682 248
Fragments arranged by size:
2559 682 248
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 3489
Size: 75 3414
PfoI T'CCnGG_A
Cuts at: 0 965 2431 3489
Size: 965 1466 1058
Fragments arranged by size:
1466 1058 965
PmlI CAC'GTG
Cuts at: 0 576 3489
Size: 576 2913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 412 444 850 882 3489
Size: 376 32 4 32 406 32 2607
Fragments arranged by size:
2607 406 376 32 32 32 4
PpuMI rG'GwC_Cy
Cuts at: 0 414 561 1455 2319 2617 2862 3489
Size: 414 147 894 864 298 245 627
Fragments arranged by size:
894 864 627 414 298 245 147
PshAI GACnn'nnGTC
Cuts at: 0 1877 3489
Size: 1877 1612
PspOMI G'GGCC_C
Cuts at: 0 682 1123 1423 1658 2448 2707 3341 3489
Size: 682 441 300 235 790 259 634 148
Fragments arranged by size:
790 682 634 441 300 259 235 148
PstI C_TGCA'G
Cuts at: 0 2121 2261 2296 3489
Size: 2121 140 35 1193
Fragments arranged by size:
2121 1193 140 35
PvuII CAG'CTG
Cuts at: 0 1260 2357 3489
Size: 1260 1097 1132
Fragments arranged by size:
1260 1132 1097
SacI G_AGCT'C
Cuts at: 0 142 1862 3489
Size: 142 1720 1627
Fragments arranged by size:
1720 1627 142
SacII CC_GC'GG
Cuts at: 0 1432 3489
Size: 1432 2057
SalI G'TCGA_C
Cuts at: 0 2214 3489
Size: 2214 1275
SanDI GG'GwC_CC
Cuts at: 0 1455 2862 3489
Size: 1455 1407 627
Fragments arranged by size:
1455 1407 627
SapI GCTCTTCn'nnn_
Cuts at: 0 2556 3489
Size: 2556 933
SbfI CC_TGCA'GG
Cuts at: 0 2261 3489
Size: 2261 1228
SexAI A'CCwGG_T
Cuts at: 0 778 3489
Size: 778 2711
SfcI C'TryA_G
Cuts at: 0 2117 2257 2292 3489
Size: 2117 140 35 1197
Fragments arranged by size:
2117 1197 140 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 397 433 1288 3079 3489
Size: 397 36 855 1791 410
Fragments arranged by size:
1791 855 410 397 36
SfoI GGC'GCC
Cuts at: 0 299 408 1791 2636 3104 3489
Size: 299 109 1383 845 468 385
Fragments arranged by size:
1383 845 468 385 299 109
SgrAI Cr'CCGG_yG
Cuts at: 0 1915 3489
Size: 1915 1574
SmaI CCC'GGG
Cuts at: 0 500 735 1742 2759 2812 3489
Size: 500 235 1007 1017 53 677
Fragments arranged by size:
1017 1007 677 500 235 53
SmlI C'TyrA_G
Cuts at: 0 871 1300 3489
Size: 871 429 2189
Fragments arranged by size:
2189 871 429
SphI G_CATG'C
Cuts at: 0 494 2589 3489
Size: 494 2095 900
Fragments arranged by size:
2095 900 494
SrfI GCCC'GGGC
Cuts at: 0 2812 3489
Size: 2812 677
StuI AGG'CCT
Cuts at: 0 700 766 1252 3489
Size: 700 66 486 2237
Fragments arranged by size:
2237 700 486 66
StyI C'CwwG_G
Cuts at: 0 509 1925 3489
Size: 509 1416 1564
Fragments arranged by size:
1564 1416 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 1870 3489
Size: 295 1575 1619
Fragments arranged by size:
1619 1575 295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 730 762 799 831 1202 1234
Size: 196 32 502 32 37 32 371 32
Cuts at: 1234 3489
Size: 2255
Fragments arranged by size:
2255 502 371 196 37 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1234 3489
Size: 1234 2255
XbaI T'CTAG_A
Cuts at: 0 1981 3489
Size: 1981 1508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 76 930 957 2686 3489
Size: 76 854 27 1729 803
Fragments arranged by size:
1729 854 803 76 27
XmaI C'CCGG_G
Cuts at: 0 498 733 1740 2757 2810 3489
Size: 498 235 1007 1017 53 679
Fragments arranged by size:
1017 1007 679 498 235 53
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI
BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AscI AseI AsiSI
AvrII BamHI BciVI BclI BfrBI BmrI BsiWI BspHI
BsrDI BsrGI BstBI BstXI BstZ17I BtsI ClaI CspCI
DraI DrdI EcoRI EcoRV FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PciI PmeI
PsiI PspXI PsrI PvuI RsrII ScaI SgfI SnaBI
SpeI SspI SwaI TatI XhoI XmnI ZraI