Restriction map for
pF1KSDA0223
(Linear) (Six-base) MAPSORT of: KIAA0223.seq Check: 3446 from: 1 to: 3408
KIAA0223.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1411 1818 3408
Size: 1411 407 1590
Fragments arranged by size:
1590 1411 407
AatII G_ACGT'C
Cuts at: 0 335 1939 3408
Size: 335 1604 1469
Fragments arranged by size:
1604 1469 335
AccI GT'mk_AC
Cuts at: 0 2162 3026 3408
Size: 2162 864 382
Fragments arranged by size:
2162 864 382
Acc65I G'GTAC_C
Cuts at: 0 1948 3408
Size: 1948 1460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 1985 2271 2602 3408
Size: 624 1361 286 331 806
Fragments arranged by size:
1361 806 624 331 286
AfeI AGC'GCT
Cuts at: 0 829 1234 3408
Size: 829 405 2174
Fragments arranged by size:
2174 829 405
AflII C'TTAA_G
Cuts at: 0 397 3408
Size: 397 3011
AflIII A'CryG_T
Cuts at: 0 2409 3408
Size: 2409 999
AhdI GACnn_n'nnGTC
Cuts at: 0 979 3408
Size: 979 2429
AleI CACnn'nnGTG
Cuts at: 0 1405 3408
Size: 1405 2003
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 1829 1861 3408
Size: 1030 32 767 32 1547
Fragments arranged by size:
1547 1030 767 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1041 1107 1437 1578 2724 3408
Size: 1041 66 330 141 1146 684
Fragments arranged by size:
1146 1041 684 330 141 66
ApaI G_GGCC'C
Cuts at: 0 450 2807 3408
Size: 450 2357 601
Fragments arranged by size:
2357 601 450
ApaLI G'TGCA_C
Cuts at: 0 1564 1663 1794 3408
Size: 1564 99 131 1614
Fragments arranged by size:
1614 1564 131 99
ApoI r'AATT_y
Cuts at: 0 3400 3408
Size: 3400 8
AvaI C'yCGr_G
Cuts at: 0 84 297 714 2038 2521 2612 2779 2944
Size: 84 213 417 1324 483 91 167 165
Cuts at: 2944 3068 3245 3408
Size: 124 177 163
Fragments arranged by size:
1324 483 417 213 177 167 165 163
124 91 84
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1436 1469 3408
Size: 1436 33 1939
Fragments arranged by size:
1939 1436 33
BanI G'GyrC_C
Cuts at: 0 319 541 1075 1163 1551 1948 2994 3408
Size: 319 222 534 88 388 397 1046 414
Fragments arranged by size:
1046 534 414 397 388 319 222 88
BanII G_rGCy'C
Cuts at: 0 26 280 450 995 1658 1682 1777 2558
Size: 26 254 170 545 663 24 95 781
Cuts at: 2558 2681 2807 3379 3408
Size: 123 126 572 29
Fragments arranged by size:
781 663 572 545 254 170 126 123
95 29 26 24
BbeI G_GCGC'C
Cuts at: 0 1555 3408
Size: 1555 1853
BbsI GAAGACnn'nnnn_
Cuts at: 0 782 1426 2910 3408
Size: 782 644 1484 498
Fragments arranged by size:
1484 782 644 498
BbvCI CC'TCA_GC
Cuts at: 0 380 2978 3154 3408
Size: 380 2598 176 254
Fragments arranged by size:
2598 380 254 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2781 2815 3408
Size: 2781 34 593
Fragments arranged by size:
2781 593 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1437 3408
Size: 1437 1971
BfrBI ATG'CAT
Cuts at: 0 483 1539 3408
Size: 483 1056 1869
Fragments arranged by size:
1869 1056 483
BglI GCCn_nnn'nGGC
Cuts at: 0 220 2605 3215 3408
Size: 220 2385 610 193
Fragments arranged by size:
2385 610 220 193
Bme1580I G_kGCm'C
Cuts at: 0 450 760 1078 1173 1568 1667 1722 1798
Size: 450 310 318 95 395 99 55 76
Cuts at: 1798 1807 2060 2246 2324 2807 3144 3408
Size: 9 253 186 78 483 337 264
Fragments arranged by size:
483 450 395 337 318 310 264 253
186 99 95 78 76 55 9
BmgBI CAC'GTC
Cuts at: 0 366 816 1686 3408
Size: 366 450 870 1722
Fragments arranged by size:
1722 870 450 366
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 2054 3382 3408
Size: 268 1786 1328 26
Fragments arranged by size:
1786 1328 268 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 363 3126 3408
Size: 363 2763 282
Fragments arranged by size:
2763 363 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 613 640 928 946 1051 1063 1372
Size: 160 453 27 288 18 105 12 309
Cuts at: 1372 1459 2239 3025 3184 3253 3408
Size: 87 780 786 159 69 155
Fragments arranged by size:
786 780 453 309 288 160 159 155
105 87 69 27 18 12
Bpu10I CC'TnA_GC
Cuts at: 0 202 380 2978 3154 3408
Size: 202 178 2598 176 254
Fragments arranged by size:
2598 254 202 178 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 409 448 2482 3408
Size: 169 240 39 2034 926
Fragments arranged by size:
2034 926 240 169 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 2932 3408
Size: 2932 476
BsaAI yAC'GTr
Cuts at: 0 1227 1629 3408
Size: 1227 402 1779
Fragments arranged by size:
1779 1227 402
BsaHI Gr'CG_yC
Cuts at: 0 164 332 785 1552 1936 2608 3408
Size: 164 168 453 767 384 672 800
Fragments arranged by size:
800 767 672 453 384 168 164
BsaWI w'CCGG_w
Cuts at: 0 272 2114 3408
Size: 272 1842 1294
Fragments arranged by size:
1842 1294 272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 919 949 2407 2437 3408
Size: 394 30 495 30 1458 30 971
Fragments arranged by size:
1458 971 495 394 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 820 1294 1297 1369 1372 1981 2841 2938
Size: 820 474 3 72 3 609 860 97
Cuts at: 2938 2941 2950 3118 3238 3408
Size: 3 9 168 120 170
Fragments arranged by size:
860 820 609 474 170 168 120 97
72 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 70 151 766 3281 3408
Size: 70 81 615 2515 127
Fragments arranged by size:
2515 615 127 81 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1249 3078 3408
Size: 1084 165 1829 330
Fragments arranged by size:
1829 1084 330 165
BsiEI CG_ry'CG
Cuts at: 0 1759 3408
Size: 1759 1649
BsiHKAI G_wGCw'C
Cuts at: 0 26 1568 1667 1798 2558 2681 3408
Size: 26 1542 99 131 760 123 727
Fragments arranged by size:
1542 760 727 131 123 99 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 517 616 1692 2215 3408
Size: 517 99 1076 523 1193
Fragments arranged by size:
1193 1076 523 517 99
Bsp1286I G_dGCh'C
Cuts at: 0 26 280 450 760 995 1078 1173 1568
Size: 26 254 170 310 235 83 95 395
Cuts at: 1568 1658 1667 1682 1722 1777 1798 1807 2060
Size: 90 9 15 40 55 21 9 253
Cuts at: 2060 2246 2324 2558 2681 2807 3144 3379 3408
Size: 186 78 234 123 126 337 235 29
Fragments arranged by size:
395 337 310 254 253 235 235 234
186 170 126 123 95 90 83 78
55 40 29 26 21 15 9 9
BspEI T'CCGG_A
Cuts at: 0 2114 3408
Size: 2114 1294
BspHI T'CATG_A
Cuts at: 0 26 3408
Size: 26 3382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1211 1411 1818 2516 2653 2699 2732 3408
Size: 1211 200 407 698 137 46 33 676
Fragments arranged by size:
1211 698 676 407 200 137 46 33
BsrBI CCG'CTC
Cuts at: 0 1005 1863 1932 2358 2529 3408
Size: 1005 858 69 426 171 879
Fragments arranged by size:
1005 879 858 426 171 69
BsrDI GCAATG_nn'
Cuts at: 0 548 3408
Size: 548 2860
BsrFI r'CCGG_y
Cuts at: 0 322 538 787 2046 2108 2618 3408
Size: 322 216 249 1259 62 510 790
Fragments arranged by size:
1259 790 510 322 249 216 62
BssHII G'CGCG_C
Cuts at: 0 1298 3408
Size: 1298 2110
BssSI C'ACGA_G
Cuts at: 0 451 1129 1264 2077 2551 3408
Size: 451 678 135 813 474 857
Fragments arranged by size:
857 813 678 474 451 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2724 3408
Size: 2724 684
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 1332 3144 3408
Size: 873 459 1812 264
Fragments arranged by size:
1812 873 459 264
BstYI r'GATC_y
Cuts at: 0 1469 3408
Size: 1469 1939
BtgI C'CryG_G
Cuts at: 0 442 518 725 1961 2821 3354 3408
Size: 442 76 207 1236 860 533 54
Fragments arranged by size:
1236 860 533 442 207 76 54
BtsI GCAGTG_nn'
Cuts at: 0 704 756 853 2651 3225 3334 3408
Size: 704 52 97 1798 574 109 74
Fragments arranged by size:
1798 704 574 109 97 74 52
EaeI y'GGCC_r
Cuts at: 0 875 1050 1268 1319 1388 1409 1511 1756
Size: 875 175 218 51 69 21 102 245
Cuts at: 1756 1858 2051 2228 2277 2567 2818 3408
Size: 102 193 177 49 290 251 590
Fragments arranged by size:
875 590 290 251 245 218 193 177
175 102 102 69 51 49 21
EagI C'GGCC_G
Cuts at: 0 1756 3408
Size: 1756 1652
EarI CTCTTCn'nnn_
Cuts at: 0 1720 2180 3408
Size: 1720 460 1228
Fragments arranged by size:
1720 1228 460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 376 1207 1291 1390 1680 2062 2530 3064
Size: 376 831 84 99 290 382 468 534
Cuts at: 3064 3378 3408
Size: 314 30
Fragments arranged by size:
831 534 468 382 376 314 290 99
84 30
EcoICRI GAG'CTC
Cuts at: 0 24 2556 2679 3408
Size: 24 2532 123 729
Fragments arranged by size:
2532 729 123 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 613 624 640 928 946 1051 1063
Size: 160 453 11 16 288 18 105 12
Cuts at: 1063 1372 1459 1985 2239 2271 2602 3025 3184
Size: 309 87 526 254 32 331 423 159
Cuts at: 3184 3253 3408
Size: 69 155
Fragments arranged by size:
526 453 423 331 309 288 254 160
159 155 105 87 69 32 18 16
12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 463 1033 1207 1907 3103 3408
Size: 427 36 570 174 700 1196 305
Fragments arranged by size:
1196 700 570 427 305 174 36
EcoO109I rG'GnC_Cy
Cuts at: 0 155 179 384 615 891 945 1038 2688
Size: 155 24 205 231 276 54 93 1650
Cuts at: 2688 3330 3408
Size: 642 78
Fragments arranged by size:
1650 642 276 231 205 155 93 78
54 24
EcoRI G'AATT_C
Cuts at: 0 3400 3408
Size: 3400 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1275 1749 3408
Size: 1275 474 1659
Fragments arranged by size:
1659 1275 474
FspI TGC'GCA
Cuts at: 0 1207 2371 3408
Size: 1207 1164 1037
Fragments arranged by size:
1207 1164 1037
HaeII r_GCGC'y
Cuts at: 0 831 1236 1462 1555 2309 2368 3408
Size: 831 405 226 93 754 59 1040
Fragments arranged by size:
1040 831 754 405 226 93 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 965 997 1180 1212 1921 1953 2071 2103
Size: 965 32 183 32 709 32 118 32
Cuts at: 2103 2947 2979 3076 3106 3108 3138 3408
Size: 844 32 97 30 2 30 270
Fragments arranged by size:
965 844 709 270 183 118 97 32
32 32 32 32 30 30 2
KasI G'GCGC_C
Cuts at: 0 1551 3408
Size: 1551 1857
KpnI G_GTAC'C
Cuts at: 0 1952 3408
Size: 1952 1456
MluI A'CGCG_T
Cuts at: 0 2409 3408
Size: 2409 999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 2990 3104 3338 3408
Size: 1223 1767 114 234 70
Fragments arranged by size:
1767 1223 234 114 70
MscI TGG'CCA
Cuts at: 0 877 1270 1390 2230 2569 3408
Size: 877 393 120 840 339 839
Fragments arranged by size:
877 840 839 393 339 120
MslI CAynn'nnrTG
Cuts at: 0 1405 3408
Size: 1405 2003
MspA1I CmG'CkG
Cuts at: 0 151 444 507 1557 1638 1644 2271 3033
Size: 151 293 63 1050 81 6 627 762
Cuts at: 3033 3215 3231 3408
Size: 182 16 177
Fragments arranged by size:
1050 762 627 293 182 177 151 81
63 16 6
NaeI GCC'GGC
Cuts at: 0 324 540 789 2620 3408
Size: 324 216 249 1831 788
Fragments arranged by size:
1831 788 324 249 216
NarI GG'CG_CC
Cuts at: 0 1552 3408
Size: 1552 1856
NcoI C'CATG_G
Cuts at: 0 725 3408
Size: 725 2683
NdeI CA'TA_TG
Cuts at: 0 1541 3408
Size: 1541 1867
NgoMIV G'CCGG_C
Cuts at: 0 322 538 787 2618 3408
Size: 322 216 249 1831 790
Fragments arranged by size:
1831 790 322 249 216
NsiI A_TGCA'T
Cuts at: 0 485 1541 3408
Size: 485 1056 1867
Fragments arranged by size:
1867 1056 485
NspI r_CATG'y
Cuts at: 0 1002 3408
Size: 1002 2406
PasI CC'CwG_GG
Cuts at: 0 3144 3408
Size: 3144 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 726 1059 1700 1987 3408
Size: 357 369 333 641 287 1421
Fragments arranged by size:
1421 641 369 357 333 287
PfoI T'CCnGG_A
Cuts at: 0 7 250 1472 3161 3408
Size: 7 243 1222 1689 247
Fragments arranged by size:
1689 1222 247 243 7
PmlI CAC'GTG
Cuts at: 0 1629 3408
Size: 1629 1779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2695 2727 3408
Size: 2695 32 681
Fragments arranged by size:
2695 681 32
PpuMI rG'GwC_Cy
Cuts at: 0 155 179 891 1038 2688 3408
Size: 155 24 712 147 1650 720
Fragments arranged by size:
1650 720 712 155 147 24
PspOMI G'GGCC_C
Cuts at: 0 446 2803 3408
Size: 446 2357 605
Fragments arranged by size:
2357 605 446
PspXI vC'TCGA_Gb
Cuts at: 0 714 3408
Size: 714 2694
PstI C_TGCA'G
Cuts at: 0 953 973 1643 2270 2720 3038 3329 3385
Size: 953 20 670 627 450 318 291 56
Cuts at: 3385 3408
Size: 23
Fragments arranged by size:
953 670 627 450 318 291 56 23
20
PvuII CAG'CTG
Cuts at: 0 1638 2271 3231 3408
Size: 1638 633 960 177
Fragments arranged by size:
1638 960 633 177
SacI G_AGCT'C
Cuts at: 0 26 2558 2681 3408
Size: 26 2532 123 727
Fragments arranged by size:
2532 727 123 26
SacII CC_GC'GG
Cuts at: 0 445 3408
Size: 445 2963
SanDI GG'GwC_CC
Cuts at: 0 179 3408
Size: 179 3229
SapI GCTCTTCn'nnn_
Cuts at: 0 1720 3408
Size: 1720 1688
SbfI CC_TGCA'GG
Cuts at: 0 3385 3408
Size: 3385 23
SexAI A'CCwGG_T
Cuts at: 0 1841 3408
Size: 1841 1567
SfcI C'TryA_G
Cuts at: 0 823 949 969 1639 2266 2716 3034 3325
Size: 823 126 20 670 627 450 318 291
Cuts at: 3325 3381 3408
Size: 56 27
Fragments arranged by size:
823 670 627 450 318 291 126 56
27 20
SfoI GGC'GCC
Cuts at: 0 1553 3408
Size: 1553 1855
SmaI CCC'GGG
Cuts at: 0 299 2614 2781 2946 3247 3408
Size: 299 2315 167 165 301 161
Fragments arranged by size:
2315 301 299 167 165 161
SmlI C'TyrA_G
Cuts at: 0 184 388 397 427 714 2497 3408
Size: 184 204 9 30 287 1783 911
Fragments arranged by size:
1783 911 287 204 184 30 9
SrfI GCCC'GGGC
Cuts at: 0 299 3408
Size: 299 3109
StuI AGG'CCT
Cuts at: 0 1159 2431 2470 3109 3408
Size: 1159 1272 39 639 299
Fragments arranged by size:
1272 1159 639 299 39
StyI C'CwwG_G
Cuts at: 0 554 725 941 1271 1448 1814 2090 2375
Size: 554 171 216 330 177 366 276 285
Cuts at: 2375 2570 2600 3408
Size: 195 30 808
Fragments arranged by size:
808 554 366 330 285 276 216 195
177 171 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 658 3408
Size: 451 207 2750
Fragments arranged by size:
2750 451 207
TatI w'GTAC_w
Cuts at: 0 881 3408
Size: 881 2527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2407 2439 3408
Size: 2407 32 969
Fragments arranged by size:
2407 969 32
Tth111I GACn'n_nGTC
Cuts at: 0 334 3408
Size: 334 3074
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1020 1329 1968 3192 3408
Size: 1020 309 639 1224 216
Fragments arranged by size:
1224 1020 639 309 216
XhoI C'TCGA_G
Cuts at: 0 714 3408
Size: 714 2694
XmaI C'CCGG_G
Cuts at: 0 297 2612 2779 2944 3245 3408
Size: 297 2315 167 165 301 163
Fragments arranged by size:
2315 301 297 167 165 163
ZraI GAC'GTC
Cuts at: 0 333 1937 3408
Size: 333 1604 1471
Fragments arranged by size:
1604 1471 333
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI
SacII SanDI SapI SbfI SexAI SfcI SfoI SmaI
SmlI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AclI AgeI AlfI AscI AseI AsiSI AvrII BamHI
BclI BglII BlpI BmtI BsaBI BsiWI BsmI BsrGI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoRV FseI FspAI HincII HindIII HpaI
MfeI NheI NotI NruI PacI PciI PmeI PshAI
PsiI PsrI PvuI RsrII SalI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI SwaI XbaI XmnI