Restriction map for
pF1KSDA0224
(Linear) (Six-base) MAPSORT of: KIAA0224.seq Check: 991 from: 1 to: 3681
KIAA0224.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1070 2187 3681
Size: 1070 1117 1494
Fragments arranged by size:
1494 1117 1070
AatII G_ACGT'C
Cuts at: 0 2431 3681
Size: 2431 1250
AccI GT'mk_AC
Cuts at: 0 637 3601 3681
Size: 637 2964 80
Fragments arranged by size:
2964 637 80
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1841 2053 2127 3681
Size: 1841 212 74 1554
Fragments arranged by size:
1841 1554 212 74
AfeI AGC'GCT
Cuts at: 0 1126 1969 3681
Size: 1126 843 1712
Fragments arranged by size:
1712 1126 843
AleI CACnn'nnGTG
Cuts at: 0 1937 1984 2644 3681
Size: 1937 47 660 1037
Fragments arranged by size:
1937 1037 660 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1529 3304 3681
Size: 1529 1775 377
Fragments arranged by size:
1775 1529 377
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 1593 1620 1704 2715 3681
Size: 1062 531 27 84 1011 966
Fragments arranged by size:
1062 1011 966 531 84 27
ApaI G_GGCC'C
Cuts at: 0 2217 2584 2796 3436 3681
Size: 2217 367 212 640 245
Fragments arranged by size:
2217 640 367 245 212
ApaLI G'TGCA_C
Cuts at: 0 1225 2200 3681
Size: 1225 975 1481
Fragments arranged by size:
1481 1225 975
ApoI r'AATT_y
Cuts at: 0 908 1552 3681
Size: 908 644 2129
Fragments arranged by size:
2129 908 644
AvaI C'yCGr_G
Cuts at: 0 338 589 749 2650 2701 2788 3681
Size: 338 251 160 1901 51 87 893
Fragments arranged by size:
1901 893 338 251 160 87 51
BamHI G'GATC_C
Cuts at: 0 1892 2783 3681
Size: 1892 891 898
Fragments arranged by size:
1892 898 891
BanI G'GyrC_C
Cuts at: 0 1236 3681
Size: 1236 2445
BanII G_rGCy'C
Cuts at: 0 598 2217 2584 2633 2796 3138 3436 3555
Size: 598 1619 367 49 163 342 298 119
Cuts at: 3555 3644 3681
Size: 89 37
Fragments arranged by size:
1619 598 367 342 298 163 119 89
49 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 849 891 1257 1853 2481 3681
Size: 105 744 42 366 596 628 1200
Fragments arranged by size:
1200 744 628 596 366 105 42
BbvCI CC'TCA_GC
Cuts at: 0 275 3681
Size: 275 3406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 949 983 1635 1669 3681
Size: 949 34 652 34 2012
Fragments arranged by size:
2012 949 652 34 34
BclI T'GATC_A
Cuts at: 0 1871 2982 3681
Size: 1871 1111 699
Fragments arranged by size:
1871 1111 699
BglI GCCn_nnn'nGGC
Cuts at: 0 3587 3681
Size: 3587 94
BglII A'GATC_T
Cuts at: 0 2522 3608 3681
Size: 2522 1086 73
Fragments arranged by size:
2522 1086 73
BlpI GC'TnA_GC
Cuts at: 0 1094 1388 3681
Size: 1094 294 2293
Fragments arranged by size:
2293 1094 294
Bme1580I G_kGCm'C
Cuts at: 0 1229 2204 2217 2584 2651 2796 3436 3681
Size: 1229 975 13 367 67 145 640 245
Fragments arranged by size:
1229 975 640 367 245 145 67 13
BmgBI CAC'GTC
Cuts at: 0 889 1986 3681
Size: 889 1097 1695
Fragments arranged by size:
1695 1097 889
BmrI ACTGGGnnnn_n'
Cuts at: 0 154 252 1410 1432 3204 3681
Size: 154 98 1158 22 1772 477
Fragments arranged by size:
1772 1158 477 154 98 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 1927 2283 3157 3522 3681
Size: 39 1888 356 874 365 159
Fragments arranged by size:
1888 874 365 356 159 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1597 2240 2311 2320 2341 2366 3602
Size: 1192 405 643 71 9 21 25 1236
Cuts at: 3602 3681
Size: 79
Fragments arranged by size:
1236 1192 643 405 79 71 25 21
9
Bpu10I CC'TnA_GC
Cuts at: 0 275 3104 3681
Size: 275 2829 577
Fragments arranged by size:
2829 577 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 2677 3127 3235 3681
Size: 657 2020 450 108 446
Fragments arranged by size:
2020 657 450 446 108
BsaAI yAC'GTr
Cuts at: 0 1041 2169 3270 3681
Size: 1041 1128 1101 411
Fragments arranged by size:
1128 1101 1041 411
BsaBI GATnn'nnATC
Cuts at: 0 2875 3681
Size: 2875 806
BsaHI Gr'CG_yC
Cuts at: 0 706 1439 2428 3681
Size: 706 733 989 1253
Fragments arranged by size:
1253 989 733 706
BsaWI w'CCGG_w
Cuts at: 0 311 3681
Size: 311 3370
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1954 1984 3681
Size: 1954 30 1697
Fragments arranged by size:
1954 1697 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 219 660 907 940 943 1017 1059
Size: 202 17 441 247 33 3 74 42
Cuts at: 1059 1834 2986 2989 3532 3535 3553 3681
Size: 775 1152 3 543 3 18 128
Fragments arranged by size:
1152 775 543 441 247 202 128 74
42 33 18 17 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1127 1748 2940 3429 3681
Size: 1127 621 1192 489 252
Fragments arranged by size:
1192 1127 621 489 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1630 1836 2728 3178 3681
Size: 1630 206 892 450 503
Fragments arranged by size:
1630 892 503 450 206
BsiEI CG_ry'CG
Cuts at: 0 969 3681
Size: 969 2712
BsiHKAI G_wGCw'C
Cuts at: 0 1229 1991 2204 2633 3138 3681
Size: 1229 762 213 429 505 543
Fragments arranged by size:
1229 762 543 505 429 213
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1660 2419 2435 3681
Size: 1660 759 16 1246
Fragments arranged by size:
1660 1246 759 16
Bsp1286I G_dGCh'C
Cuts at: 0 598 1229 1991 2204 2217 2584 2633 2651
Size: 598 631 762 213 13 367 49 18
Cuts at: 2651 2796 3138 3436 3555 3644 3681
Size: 145 342 298 119 89 37
Fragments arranged by size:
762 631 598 367 342 298 213 145
119 89 49 37 18 13
BspEI T'CCGG_A
Cuts at: 0 311 3681
Size: 311 3370
BspHI T'CATG_A
Cuts at: 0 3368 3681
Size: 3368 313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1070 1601 1712 2187 2723 3053 3681
Size: 1070 531 111 475 536 330 628
Fragments arranged by size:
1070 628 536 531 475 330 111
BsrBI CCG'CTC
Cuts at: 0 408 414 516 729 933 3681
Size: 408 6 102 213 204 2748
Fragments arranged by size:
2748 408 213 204 102 6
BsrDI GCAATG_nn'
Cuts at: 0 1325 3681
Size: 1325 2356
BsrFI r'CCGG_y
Cuts at: 0 2569 3681
Size: 2569 1112
BssSI C'ACGA_G
Cuts at: 0 1963 3180 3358 3681
Size: 1963 1217 178 323
Fragments arranged by size:
1963 1217 323 178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1062 1593 1620 1704 2193 2715 3681
Size: 1062 531 27 84 489 522 966
Fragments arranged by size:
1062 966 531 522 489 84 27
BstEII G'GTnAC_C
Cuts at: 0 289 2262 3681
Size: 289 1973 1419
Fragments arranged by size:
1973 1419 289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 364 1158 1776 1950 2113 3681
Size: 364 794 618 174 163 1568
Fragments arranged by size:
1568 794 618 364 174 163
BstYI r'GATC_y
Cuts at: 0 1892 2522 2654 2783 2909 3608 3681
Size: 1892 630 132 129 126 699 73
Fragments arranged by size:
1892 699 630 132 129 126 73
BtgI C'CryG_G
Cuts at: 0 3512 3644 3681
Size: 3512 132 37
Fragments arranged by size:
3512 132 37
BtsI GCAGTG_nn'
Cuts at: 0 946 1554 1613 2186 3055 3397 3597 3681
Size: 946 608 59 573 869 342 200 84
Fragments arranged by size:
946 869 608 573 342 200 84 59
ClaI AT'CG_AT
Cuts at: 0 33 2462 3681
Size: 33 2429 1219
Fragments arranged by size:
2429 1219 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2400 2435 2790 2825 3681
Size: 2400 35 355 35 856
Fragments arranged by size:
2400 856 355 35 35
EaeI y'GGCC_r
Cuts at: 0 95 300 1205 1319 1778 2115 2244 2672
Size: 95 205 905 114 459 337 129 428
Cuts at: 2672 3533 3681
Size: 861 148
Fragments arranged by size:
905 861 459 428 337 205 148 129
114 95
EarI CTCTTCn'nnn_
Cuts at: 0 242 275 371 644 1187 1448 1522 1790
Size: 242 33 96 273 543 261 74 268
Cuts at: 1790 2146 3681
Size: 356 1535
Fragments arranged by size:
1535 543 356 273 268 261 242 96
74 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3439 3681
Size: 3439 242
EcoICRI GAG'CTC
Cuts at: 0 2631 3136 3681
Size: 2631 505 545
Fragments arranged by size:
2631 545 505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1597 1841 2053 2127 2240 2311 2320
Size: 1192 405 244 212 74 113 71 9
Cuts at: 2320 2341 2366 3602 3681
Size: 21 25 1236 79
Fragments arranged by size:
1236 1192 405 244 212 113 79 74
71 25 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1816 3681
Size: 1816 1865
EcoO109I rG'GnC_Cy
Cuts at: 0 2213 2214 2712 2792 2793 3433 3681
Size: 2213 1 498 80 1 640 248
Fragments arranged by size:
2213 640 498 248 80 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2208 3681
Size: 2208 1473
HaeII r_GCGC'y
Cuts at: 0 681 1128 1971 2025 2618 3532 3681
Size: 681 447 843 54 593 914 149
Fragments arranged by size:
914 843 681 593 447 149 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 544 576 3600 3632 3681
Size: 337 32 175 32 3024 32 49
Fragments arranged by size:
3024 337 175 49 32 32 32
HincII GTy'rAC
Cuts at: 0 2133 2442 3681
Size: 2133 309 1239
Fragments arranged by size:
2133 1239 309
MscI TGG'CCA
Cuts at: 0 302 1321 1780 2246 2674 3681
Size: 302 1019 459 466 428 1007
Fragments arranged by size:
1019 1007 466 459 428 302
MslI CAynn'nnrTG
Cuts at: 0 362 1712 1774 1937 1948 1984 2260 2644
Size: 362 1350 62 163 11 36 276 384
Cuts at: 2644 3154 3286 3681
Size: 510 132 395
Fragments arranged by size:
1350 510 395 384 362 276 163 132
62 36 11
MspA1I CmG'CkG
Cuts at: 0 1017 1098 1692 2343 2556 2850 3165 3403
Size: 1017 81 594 651 213 294 315 238
Cuts at: 3403 3546 3681
Size: 143 135
Fragments arranged by size:
1017 651 594 315 294 238 213 143
135 81
NaeI GCC'GGC
Cuts at: 0 2571 3681
Size: 2571 1110
NcoI C'CATG_G
Cuts at: 0 3512 3644 3681
Size: 3512 132 37
Fragments arranged by size:
3512 132 37
NgoMIV G'CCGG_C
Cuts at: 0 2569 3681
Size: 2569 1112
NspI r_CATG'y
Cuts at: 0 112 1146 2760 3681
Size: 112 1034 1614 921
Fragments arranged by size:
1614 1034 921 112
PasI CC'CwG_GG
Cuts at: 0 3585 3681
Size: 3585 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 1170 2280 2733 3077 3681
Size: 36 1134 1110 453 344 604
Fragments arranged by size:
1134 1110 604 453 344 36
PfoI T'CCnGG_A
Cuts at: 0 1574 1913 2003 2990 3122 3681
Size: 1574 339 90 987 132 559
Fragments arranged by size:
1574 987 559 339 132 90
PpuMI rG'GwC_Cy
Cuts at: 0 2712 3681
Size: 2712 969
PspOMI G'GGCC_C
Cuts at: 0 2213 2580 2792 3432 3681
Size: 2213 367 212 640 249
Fragments arranged by size:
2213 640 367 249 212
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3049 3081 3681
Size: 3049 32 600
Fragments arranged by size:
3049 600 32
PstI C_TGCA'G
Cuts at: 0 2181 2522 2723 2902 3050 3681
Size: 2181 341 201 179 148 631
Fragments arranged by size:
2181 631 341 201 179 148
PvuI CG_AT'CG
Cuts at: 0 969 3681
Size: 969 2712
PvuII CAG'CTG
Cuts at: 0 1692 2343 3546 3681
Size: 1692 651 1203 135
Fragments arranged by size:
1692 1203 651 135
SacI G_AGCT'C
Cuts at: 0 2633 3138 3681
Size: 2633 505 543
Fragments arranged by size:
2633 543 505
SapI GCTCTTCn'nnn_
Cuts at: 0 1448 3681
Size: 1448 2233
ScaI AGT'ACT
Cuts at: 0 781 3681
Size: 781 2900
SfcI C'TryA_G
Cuts at: 0 1494 1935 2177 2518 2719 2898 2952 3046
Size: 1494 441 242 341 201 179 54 94
Cuts at: 3046 3444 3681
Size: 398 237
Fragments arranged by size:
1494 441 398 341 242 237 201 179
94 54
SmlI C'TyrA_G
Cuts at: 0 672 749 2692 3142 3250 3681
Size: 672 77 1943 450 108 431
Fragments arranged by size:
1943 672 450 431 108 77
SphI G_CATG'C
Cuts at: 0 1146 3681
Size: 1146 2535
SspI AAT'ATT
Cuts at: 0 2418 3681
Size: 2418 1263
StyI C'CwwG_G
Cuts at: 0 1208 1812 2960 3377 3512 3644 3681
Size: 1208 604 1148 417 135 132 37
Fragments arranged by size:
1208 1148 604 417 135 132 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 320 1730 2623 2925 3681
Size: 320 1410 893 302 756
Fragments arranged by size:
1410 893 756 320 302
TatI w'GTAC_w
Cuts at: 0 779 3681
Size: 779 2902
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 838 870 1954 1986 3400 3432 3681
Size: 838 32 1084 32 1414 32 249
Fragments arranged by size:
1414 1084 838 249 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3202 3681
Size: 3202 479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1314 2505 3681
Size: 1314 1191 1176
Fragments arranged by size:
1314 1191 1176
XhoI C'TCGA_G
Cuts at: 0 749 3681
Size: 749 2932
ZraI GAC'GTC
Cuts at: 0 2429 3681
Size: 2429 1252
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI ClaI CspCI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PsrI
PstI PvuI PvuII SacI SapI ScaI SfcI SmlI
SphI SspI StyI TaqII TatI TstI Tth111I XcmI
XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AloI AscI
AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BmtI
BsaI BsiWI BsmI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI DraI DraIII DrdI EagI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI XbaI
XmaI XmnI