Restriction map for
pF1KA0227
(Linear) (Six-base) MAPSORT of: KIAA0227.seq Check: 8837 from: 1 to: 666
KIAA0227.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 349 666
Size: 280 69 317
Fragments arranged by size:
317 280 69
AgeI A'CCGG_T
Cuts at: 0 503 666
Size: 503 163
AleI CACnn'nnGTG
Cuts at: 0 595 666
Size: 595 71
ApaI G_GGCC'C
Cuts at: 0 327 666
Size: 327 339
ApoI r'AATT_y
Cuts at: 0 214 666
Size: 214 452
AvaI C'yCGr_G
Cuts at: 0 39 93 280 666
Size: 39 54 187 386
Fragments arranged by size:
386 187 54 39
BanII G_rGCy'C
Cuts at: 0 16 46 114 158 327 666
Size: 16 30 68 44 169 339
Fragments arranged by size:
339 169 68 44 30 16
BglI GCCn_nnn'nGGC
Cuts at: 0 148 316 666
Size: 148 168 350
Fragments arranged by size:
350 168 148
BlpI GC'TnA_GC
Cuts at: 0 422 666
Size: 422 244
Bme1580I G_kGCm'C
Cuts at: 0 327 666
Size: 327 339
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 666
Size: 315 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 400 666
Size: 274 126 266
Fragments arranged by size:
274 266 126
BsaWI w'CCGG_w
Cuts at: 0 503 666
Size: 503 163
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 364 666
Size: 122 242 302
Fragments arranged by size:
302 242 122
BseYI C'CCAG_C
Cuts at: 0 114 319 666
Size: 114 205 347
Fragments arranged by size:
347 205 114
BsiEI CG_ry'CG
Cuts at: 0 23 56 143 152 339 666
Size: 23 33 87 9 187 327
Fragments arranged by size:
327 187 87 33 23 9
BsiHKAI G_wGCw'C
Cuts at: 0 158 666
Size: 158 508
Bsp1286I G_dGCh'C
Cuts at: 0 16 46 114 158 327 666
Size: 16 30 68 44 169 339
Fragments arranged by size:
339 169 68 44 30 16
BsrBI CCG'CTC
Cuts at: 0 294 666
Size: 294 372
BsrFI r'CCGG_y
Cuts at: 0 147 503 666
Size: 147 356 163
Fragments arranged by size:
356 163 147
BssHII G'CGCG_C
Cuts at: 0 303 666
Size: 303 363
BssSI C'ACGA_G
Cuts at: 0 172 666
Size: 172 494
BtgI C'CryG_G
Cuts at: 0 50 666
Size: 50 616
BtsI GCAGTG_nn'
Cuts at: 0 202 666
Size: 202 464
EaeI y'GGCC_r
Cuts at: 0 20 53 70 140 149 336 666
Size: 20 33 17 70 9 187 330
Fragments arranged by size:
330 187 70 33 20 17 9
EagI C'GGCC_G
Cuts at: 0 20 53 140 149 336 666
Size: 20 33 87 9 187 330
Fragments arranged by size:
330 187 87 33 20 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 119 666
Size: 119 547
EcoICRI GAG'CTC
Cuts at: 0 156 666
Size: 156 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 280 349 400 666
Size: 274 6 69 51 266
Fragments arranged by size:
274 266 69 51 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 415 666
Size: 346 69 251
Fragments arranged by size:
346 251 69
EcoO109I rG'GnC_Cy
Cuts at: 0 323 324 495 572 666
Size: 323 1 171 77 94
Fragments arranged by size:
323 171 94 77 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 666
Size: 186 480
MslI CAynn'nnrTG
Cuts at: 0 595 666
Size: 595 71
MspA1I CmG'CkG
Cuts at: 0 52 69 84 615 666
Size: 52 17 15 531 51
Fragments arranged by size:
531 52 51 17 15
NaeI GCC'GGC
Cuts at: 0 149 666
Size: 149 517
NgoMIV G'CCGG_C
Cuts at: 0 147 666
Size: 147 519
PpuMI rG'GwC_Cy
Cuts at: 0 572 666
Size: 572 94
PspOMI G'GGCC_C
Cuts at: 0 323 666
Size: 323 343
PvuII CAG'CTG
Cuts at: 0 615 666
Size: 615 51
SacI G_AGCT'C
Cuts at: 0 158 666
Size: 158 508
SacII CC_GC'GG
Cuts at: 0 53 666
Size: 53 613
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 148 666
Size: 148 518
SmaI CCC'GGG
Cuts at: 0 95 282 666
Size: 95 187 384
Fragments arranged by size:
384 187 95
SspI AAT'ATT
Cuts at: 0 454 666
Size: 454 212
StyI C'CwwG_G
Cuts at: 0 29 186 528 666
Size: 29 157 342 138
Fragments arranged by size:
342 157 138 29
XmaI C'CCGG_G
Cuts at: 0 93 280 666
Size: 93 187 386
Fragments arranged by size:
386 187 93
Enzymes that do cut:
AcuI AgeI AleI ApaI ApoI AvaI BanII BglI
BlpI Bme1580I BplI BpmI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI BtgI BtsI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I MmeI
MslI MspA1I NaeI NgoMIV PpuMI PspOMI PvuII SacI
SacII SfiI SmaI SspI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI