Restriction map for
pF1KA0229
(Linear) (Six-base) MAPSORT of: KIAA0229.seq Check: 8435 from: 1 to: 3402
KIAA0229.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AatII G_ACGT'C
Cuts at: 0 818 2569 3402
Size: 818 1751 833
Fragments arranged by size:
1751 833 818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 2461 2682 3402
Size: 1085 1376 221 720
Fragments arranged by size:
1376 1085 720 221
AfeI AGC'GCT
Cuts at: 0 2695 3402
Size: 2695 707
AgeI A'CCGG_T
Cuts at: 0 3264 3402
Size: 3264 138
AleI CACnn'nnGTG
Cuts at: 0 487 1775 3402
Size: 487 1288 1627
Fragments arranged by size:
1627 1288 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 275 766 776 3402
Size: 275 491 10 2626
Fragments arranged by size:
2626 491 275 10
AlwNI CAG_nnn'CTG
Cuts at: 0 18 811 1033 1297 2211 3402
Size: 18 793 222 264 914 1191
Fragments arranged by size:
1191 914 793 264 222 18
ApoI r'AATT_y
Cuts at: 0 541 3402
Size: 541 2861
AseI AT'TA_AT
Cuts at: 0 901 3402
Size: 901 2501
AvaI C'yCGr_G
Cuts at: 0 1340 2684 3278 3402
Size: 1340 1344 594 124
Fragments arranged by size:
1344 1340 594 124
AvrII C'CTAG_G
Cuts at: 0 1207 1318 3402
Size: 1207 111 2084
Fragments arranged by size:
2084 1207 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3042 3075 3402
Size: 3042 33 327
Fragments arranged by size:
3042 327 33
BamHI G'GATC_C
Cuts at: 0 2702 3402
Size: 2702 700
BanI G'GyrC_C
Cuts at: 0 41 1603 2543 2648 2676 3223 3267 3402
Size: 41 1562 940 105 28 547 44 135
Fragments arranged by size:
1562 940 547 135 105 44 41 28
BanII G_rGCy'C
Cuts at: 0 2429 2845 3402
Size: 2429 416 557
Fragments arranged by size:
2429 557 416
BbeI G_GCGC'C
Cuts at: 0 1607 2652 3227 3402
Size: 1607 1045 575 175
Fragments arranged by size:
1607 1045 575 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 1265 1355 3402
Size: 1265 90 2047
Fragments arranged by size:
2047 1265 90
BbvCI CC'TCA_GC
Cuts at: 0 1172 1784 2539 3391 3402
Size: 1172 612 755 852 11
Fragments arranged by size:
1172 852 755 612 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2109 2143 3402
Size: 2109 34 1259
Fragments arranged by size:
2109 1259 34
BclI T'GATC_A
Cuts at: 0 2852 3402
Size: 2852 550
BglI GCCn_nnn'nGGC
Cuts at: 0 2331 3199 3402
Size: 2331 868 203
Fragments arranged by size:
2331 868 203
BglII A'GATC_T
Cuts at: 0 2069 2613 2859 3402
Size: 2069 544 246 543
Fragments arranged by size:
2069 544 543 246
BlpI GC'TnA_GC
Cuts at: 0 193 3402
Size: 193 3209
Bme1580I G_kGCm'C
Cuts at: 0 44 1677 2327 3402
Size: 44 1633 650 1075
Fragments arranged by size:
1633 1075 650 44
BmgBI CAC'GTC
Cuts at: 0 1017 3402
Size: 1017 2385
BmrI ACTGGGnnnn_n'
Cuts at: 0 1664 2609 2764 3402
Size: 1664 945 155 638
Fragments arranged by size:
1664 945 638 155
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1512 3402
Size: 1512 1890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 168 604 963 1211 1217 1651 1676
Size: 43 125 436 359 248 6 434 25
Cuts at: 1676 2110 2134 2242 2612 3402
Size: 434 24 108 370 790
Fragments arranged by size:
790 436 434 434 370 359 248 125
108 43 25 24 6
Bpu10I CC'TnA_GC
Cuts at: 0 625 1172 1784 2539 3272 3391 3402
Size: 625 547 612 755 733 119 11
Fragments arranged by size:
755 733 625 612 547 119 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 541 1308 1787 3402
Size: 541 767 479 1615
Fragments arranged by size:
1615 767 541 479
BsaHI Gr'CG_yC
Cuts at: 0 815 1604 2566 2649 2888 3224 3402
Size: 815 789 962 83 239 336 178
Fragments arranged by size:
962 815 789 336 239 178 83
BsaWI w'CCGG_w
Cuts at: 0 1796 3017 3264 3402
Size: 1796 1221 247 138
Fragments arranged by size:
1796 1221 247 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 1138 1168 1501 1531 1749 1779
Size: 166 30 942 30 333 30 218 30
Cuts at: 1779 1917 1947 3402
Size: 138 30 1455
Fragments arranged by size:
1455 942 333 218 166 138 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 523 691 1495 1506 1648 2518 2725
Size: 75 448 168 804 11 142 870 207
Cuts at: 2725 3402
Size: 677
Fragments arranged by size:
870 804 677 448 207 168 142 75
11
BseYI C'CCAG_C
Cuts at: 0 962 1595 1664 2534 3159 3402
Size: 962 633 69 870 625 243
Fragments arranged by size:
962 870 633 625 243 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 485 640 1355 2580 2794 3402
Size: 235 250 155 715 1225 214 608
Fragments arranged by size:
1225 715 608 250 235 214 155
BsiEI CG_ry'CG
Cuts at: 0 2608 3402
Size: 2608 794
BsiHKAI G_wGCw'C
Cuts at: 0 503 2429 2717 2921 3011 3402
Size: 503 1926 288 204 90 391
Fragments arranged by size:
1926 503 391 288 204 90
BsiWI C'GTAC_G
Cuts at: 0 2498 3402
Size: 2498 904
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1630 3402
Size: 1630 1772
Bsp1286I G_dGCh'C
Cuts at: 0 44 503 1677 2327 2429 2717 2845 2921
Size: 44 459 1174 650 102 288 128 76
Cuts at: 2921 3011 3402
Size: 90 391
Fragments arranged by size:
1174 650 459 391 288 128 102 90
76 44
BspEI T'CCGG_A
Cuts at: 0 3017 3402
Size: 3017 385
BspHI T'CATG_A
Cuts at: 0 2021 3402
Size: 2021 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1200 1470 1903 2219 3092 3402
Size: 1200 270 433 316 873 310
Fragments arranged by size:
1200 873 433 316 310 270
BsrBI CCG'CTC
Cuts at: 0 1733 2689 3402
Size: 1733 956 713
Fragments arranged by size:
1733 956 713
BsrDI GCAATG_nn'
Cuts at: 0 2999 3402
Size: 2999 403
BsrFI r'CCGG_y
Cuts at: 0 2243 3264 3402
Size: 2243 1021 138
Fragments arranged by size:
2243 1021 138
BssSI C'ACGA_G
Cuts at: 0 3010 3402
Size: 3010 392
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 795 2211 3402
Size: 18 777 1416 1191
Fragments arranged by size:
1416 1191 777 18
BstBI TT'CG_AA
Cuts at: 0 3173 3402
Size: 3173 229
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1677 2838 3402
Size: 1677 1161 564
Fragments arranged by size:
1677 1161 564
BstYI r'GATC_y
Cuts at: 0 2069 2613 2702 2859 2930 3014 3402
Size: 2069 544 89 157 71 84 388
Fragments arranged by size:
2069 544 388 157 89 84 71
Bsu36I CC'TnA_GG
Cuts at: 0 87 3402
Size: 87 3315
BtgI C'CryG_G
Cuts at: 0 686 834 1509 1919 3122 3402
Size: 686 148 675 410 1203 280
Fragments arranged by size:
1203 686 675 410 280 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2446 2460 3402
Size: 2446 14 942
Fragments arranged by size:
2446 942 14
BtsI GCAGTG_nn'
Cuts at: 0 750 1034 3402
Size: 750 284 2368
Fragments arranged by size:
2368 750 284
ClaI AT'CG_AT
Cuts at: 0 2978 3402
Size: 2978 424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1376 1411 3085 3120 3402
Size: 1376 35 1674 35 282
Fragments arranged by size:
1674 1376 282 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1480 3402
Size: 1480 1922
EaeI y'GGCC_r
Cuts at: 0 270 477 675 1127 1569 2457 3402
Size: 270 207 198 452 442 888 945
Fragments arranged by size:
945 888 452 442 270 207 198
EarI CTCTTCn'nnn_
Cuts at: 0 171 1064 1244 2195 3402
Size: 171 893 180 951 1207
Fragments arranged by size:
1207 951 893 180 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1446 2262 3402
Size: 1446 816 1140
Fragments arranged by size:
1446 1140 816
EcoICRI GAG'CTC
Cuts at: 0 2427 3402
Size: 2427 975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 168 604 963 1085 1211 1217 1651
Size: 43 125 436 359 122 126 6 434
Cuts at: 1651 1676 2110 2134 2242 2461 2612 2682 3402
Size: 25 434 24 108 219 151 70 720
Fragments arranged by size:
720 436 434 434 359 219 151 126
125 122 108 70 43 25 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2278 3402
Size: 2278 1124
EcoO109I rG'GnC_Cy
Cuts at: 0 406 696 1075 1482 1542 1589 2208 3042
Size: 406 290 379 407 60 47 619 834
Cuts at: 3042 3051 3081 3402
Size: 9 30 321
Fragments arranged by size:
834 619 407 406 379 321 290 60
47 30 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 519 3402
Size: 519 2883
FspI TGC'GCA
Cuts at: 0 532 3402
Size: 532 2870
HaeII r_GCGC'y
Cuts at: 0 1607 1822 2652 2697 3227 3402
Size: 1607 215 830 45 530 175
Fragments arranged by size:
1607 830 530 215 175 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1138 1170 1291 1323 1927 1959
Size: 979 32 127 32 121 32 604 32
Cuts at: 1959 2542 2574 2844 2876 3319 3351 3402
Size: 583 32 270 32 443 32 51
Fragments arranged by size:
979 604 583 443 270 127 121 51
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 228 819 1376 3402
Size: 228 591 557 2026
Fragments arranged by size:
2026 591 557 228
HpaI GTT'AAC
Cuts at: 0 1376 3402
Size: 1376 2026
KasI G'GCGC_C
Cuts at: 0 1603 2648 3223 3402
Size: 1603 1045 575 179
Fragments arranged by size:
1603 1045 575 179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1909 1970 3011 3402
Size: 1097 812 61 1041 391
Fragments arranged by size:
1097 1041 812 391 61
MscI TGG'CCA
Cuts at: 0 272 479 677 1129 1571 3402
Size: 272 207 198 452 442 1831
Fragments arranged by size:
1831 452 442 272 207 198
MslI CAynn'nnrTG
Cuts at: 0 278 487 1331 1361 1775 3344 3402
Size: 278 209 844 30 414 1569 58
Fragments arranged by size:
1569 844 414 278 209 58 30
MspA1I CmG'CkG
Cuts at: 0 140 1133 1554 1567 1629 2451 2538 3235
Size: 140 993 421 13 62 822 87 697
Cuts at: 3235 3402
Size: 167
Fragments arranged by size:
993 822 697 421 167 140 87 62
13
NarI GG'CG_CC
Cuts at: 0 1604 2649 3224 3402
Size: 1604 1045 575 178
Fragments arranged by size:
1604 1045 575 178
NcoI C'CATG_G
Cuts at: 0 834 1919 3402
Size: 834 1085 1483
Fragments arranged by size:
1483 1085 834
NspI r_CATG'y
Cuts at: 0 201 1626 1651 3402
Size: 201 1425 25 1751
Fragments arranged by size:
1751 1425 201 25
PasI CC'CwG_GG
Cuts at: 0 2343 3402
Size: 2343 1059
PflMI CCAn_nnn'nTGG
Cuts at: 0 1116 2181 3402
Size: 1116 1065 1221
Fragments arranged by size:
1221 1116 1065
PfoI T'CCnGG_A
Cuts at: 0 70 1653 2705 3402
Size: 70 1583 1052 697
Fragments arranged by size:
1583 1052 697 70
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1838 1870 3402
Size: 1838 32 1532
Fragments arranged by size:
1838 1532 32
PpuMI rG'GwC_Cy
Cuts at: 0 696 1075 1482 1542 1589 2208 3042 3051
Size: 696 379 407 60 47 619 834 9
Cuts at: 3051 3081 3402
Size: 30 321
Fragments arranged by size:
834 696 619 407 379 321 60 47
30 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3124 3156 3402
Size: 3124 32 246
Fragments arranged by size:
3124 246 32
PstI C_TGCA'G
Cuts at: 0 807 1954 2132 2354 3089 3200 3234 3402
Size: 807 1147 178 222 735 111 34 168
Fragments arranged by size:
1147 807 735 222 178 168 111 34
PvuII CAG'CTG
Cuts at: 0 1554 1629 2451 2538 3235 3402
Size: 1554 75 822 87 697 167
Fragments arranged by size:
1554 822 697 167 87 75
SacI G_AGCT'C
Cuts at: 0 2429 3402
Size: 2429 973
SanDI GG'GwC_CC
Cuts at: 0 1075 1589 3402
Size: 1075 514 1813
Fragments arranged by size:
1813 1075 514
SapI GCTCTTCn'nnn_
Cuts at: 0 171 2195 3402
Size: 171 2024 1207
Fragments arranged by size:
2024 1207 171
SbfI CC_TGCA'GG
Cuts at: 0 3200 3402
Size: 3200 202
SfcI C'TryA_G
Cuts at: 0 803 1950 2128 2350 3085 3196 3230 3402
Size: 803 1147 178 222 735 111 34 172
Fragments arranged by size:
1147 803 735 222 178 172 111 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3199 3402
Size: 3199 203
SfoI GGC'GCC
Cuts at: 0 1605 2650 3225 3402
Size: 1605 1045 575 177
Fragments arranged by size:
1605 1045 575 177
SgrAI Cr'CCGG_yG
Cuts at: 0 2243 3402
Size: 2243 1159
SmaI CCC'GGG
Cuts at: 0 1342 3402
Size: 1342 2060
SphI G_CATG'C
Cuts at: 0 1626 1651 3402
Size: 1626 25 1751
Fragments arranged by size:
1751 1626 25
StuI AGG'CCT
Cuts at: 0 1206 1502 1712 3169 3402
Size: 1206 296 210 1457 233
Fragments arranged by size:
1457 1206 296 233 210
StyI C'CwwG_G
Cuts at: 0 834 1207 1318 1919 2279 3077 3402
Size: 834 373 111 601 360 798 325
Fragments arranged by size:
834 798 601 373 360 325 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 793 1917 3402
Size: 793 1124 1485
Fragments arranged by size:
1485 1124 793
TatI w'GTAC_w
Cuts at: 0 931 3402
Size: 931 2471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 1501 1533 1749 1781 1917 1949
Size: 166 32 1303 32 216 32 136 32
Cuts at: 1949 3402
Size: 1453
Fragments arranged by size:
1453 1303 216 166 136 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2170 2359 3402
Size: 2170 189 1043
Fragments arranged by size:
2170 1043 189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1626 2777 3402
Size: 1626 1151 625
Fragments arranged by size:
1626 1151 625
XmaI C'CCGG_G
Cuts at: 0 1340 3402
Size: 1340 2062
ZraI GAC'GTC
Cuts at: 0 816 2567 3402
Size: 816 1751 835
Fragments arranged by size:
1751 835 816
Enzymes that do cut:
AatII AcuI AfeI AgeI AleI AlfI AlwNI ApoI
AseI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HpaI
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PpiI PpuMI PsrI PstI PvuII
SacI SanDI SapI SbfI SfcI SfiI SfoI SgrAI
SmaI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AhdI AloI
ApaI ApaLI AscI AsiSI BciVI BfrBI BmtI BpuEI
BsaAI BsaBI BsmI BsrGI BssHII BstEII BstZ17I DraI
DraIII EagI EcoRI EcoRV FseI FspAI HindIII KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI ScaI SexAI SgfI
SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
XmnI