Restriction map for
pF1KSDA0230
(Linear) (Six-base) MAPSORT of: KIAA0230.seq Check: 6271 from: 1 to: 4437
KIAA0230.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 805 4342 4437
Size: 805 3537 95
Fragments arranged by size:
3537 805 95
AatII G_ACGT'C
Cuts at: 0 1847 3805 4437
Size: 1847 1958 632
Fragments arranged by size:
1958 1847 632
AccI GT'mk_AC
Cuts at: 0 2087 3524 4437
Size: 2087 1437 913
Fragments arranged by size:
2087 1437 913
AclI AA'CG_TT
Cuts at: 0 1835 4437
Size: 1835 2602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1745 3192 4101 4137 4437
Size: 1745 1447 909 36 300
Fragments arranged by size:
1745 1447 909 300 36
AfeI AGC'GCT
Cuts at: 0 10 4437
Size: 10 4427
AflIII A'CryG_T
Cuts at: 0 2201 2546 2741 3548 3860 4437
Size: 2201 345 195 807 312 577
Fragments arranged by size:
2201 807 577 345 312 195
AhdI GACnn_n'nnGTC
Cuts at: 0 2124 4437
Size: 2124 2313
AleI CACnn'nnGTG
Cuts at: 0 136 1573 1603 2497 3847 4437
Size: 136 1437 30 894 1350 590
Fragments arranged by size:
1437 1350 894 590 136 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 169 1162 3992 4437
Size: 154 15 993 2830 445
Fragments arranged by size:
2830 993 445 154 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3412 3444 4437
Size: 3412 32 993
Fragments arranged by size:
3412 993 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2139 2472 3402 4437
Size: 2139 333 930 1035
Fragments arranged by size:
2139 1035 930 333
ApaI G_GGCC'C
Cuts at: 0 22 2619 3688 4437
Size: 22 2597 1069 749
Fragments arranged by size:
2597 1069 749 22
ApoI r'AATT_y
Cuts at: 0 330 1708 4437
Size: 330 1378 2729
Fragments arranged by size:
2729 1378 330
AvaI C'yCGr_G
Cuts at: 0 22 478 774 1654 2333 2600 3845 4437
Size: 22 456 296 880 679 267 1245 592
Fragments arranged by size:
1245 880 679 592 456 296 267 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 921 954 4437
Size: 921 33 3483
Fragments arranged by size:
3483 921 33
BamHI G'GATC_C
Cuts at: 0 1995 3497 3815 4437
Size: 1995 1502 318 622
Fragments arranged by size:
1995 1502 622 318
BanI G'GyrC_C
Cuts at: 0 26 180 1433 1615 2236 2269 3666 4437
Size: 26 154 1253 182 621 33 1397 771
Fragments arranged by size:
1397 1253 771 621 182 154 33 26
BanII G_rGCy'C
Cuts at: 0 22 755 1655 2619 2899 3416 3688 4437
Size: 22 733 900 964 280 517 272 749
Fragments arranged by size:
964 900 749 733 517 280 272 22
BbeI G_GCGC'C
Cuts at: 0 30 184 1619 2273 4437
Size: 30 154 1435 654 2164
Fragments arranged by size:
2164 1435 654 154 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 326 871 985 3694 3956 4186 4437
Size: 326 545 114 2709 262 230 251
Fragments arranged by size:
2709 545 326 262 251 230 114
BbvCI CC'TCA_GC
Cuts at: 0 253 4110 4437
Size: 253 3857 327
Fragments arranged by size:
3857 327 253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2141 2175 2613 2647 4437
Size: 2141 34 438 34 1790
Fragments arranged by size:
2141 1790 438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 293 1997 4437
Size: 293 1704 2440
Fragments arranged by size:
2440 1704 293
BfrBI ATG'CAT
Cuts at: 0 150 4135 4297 4437
Size: 150 3985 162 140
Fragments arranged by size:
3985 162 150 140
BglI GCCn_nnn'nGGC
Cuts at: 0 82 2798 3202 3683 4437
Size: 82 2716 404 481 754
Fragments arranged by size:
2716 754 481 404 82
BglII A'GATC_T
Cuts at: 0 201 4437
Size: 201 4236
BlpI GC'TnA_GC
Cuts at: 0 856 4437
Size: 856 3581
Bme1580I G_kGCm'C
Cuts at: 0 22 173 2369 2619 3392 3688 4437
Size: 22 151 2196 250 773 296 749
Fragments arranged by size:
2196 773 749 296 250 151 22
BmgBI CAC'GTC
Cuts at: 0 3861 4437
Size: 3861 576
BmrI ACTGGGnnnn_n'
Cuts at: 0 1567 3033 4064 4437
Size: 1567 1466 1031 373
Fragments arranged by size:
1567 1466 1031 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 998 1102 2625 4437
Size: 181 817 104 1523 1812
Fragments arranged by size:
1812 1523 817 181 104
Bpu10I CC'TnA_GC
Cuts at: 0 253 2506 4110 4437
Size: 253 2253 1604 327
Fragments arranged by size:
2253 1604 327 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2992 4033 4437
Size: 2992 1041 404
Fragments arranged by size:
2992 1041 404
BsaI GGTCTCn'nnnn_
Cuts at: 0 1016 2410 3043 4013 4437
Size: 1016 1394 633 970 424
Fragments arranged by size:
1394 1016 970 633 424
BsaHI Gr'CG_yC
Cuts at: 0 27 181 1312 1616 1844 2270 3802 4437
Size: 27 154 1131 304 228 426 1532 635
Fragments arranged by size:
1532 1131 635 426 304 228 154 27
BsaWI w'CCGG_w
Cuts at: 0 503 1982 2222 3317 4437
Size: 503 1479 240 1095 1120
Fragments arranged by size:
1479 1120 1095 503 240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2473 2503 3412 3442 4437
Size: 2473 30 909 30 995
Fragments arranged by size:
2473 995 909 30 30
BseYI C'CCAG_C
Cuts at: 0 1591 2943 3138 3681 3779 4437
Size: 1591 1352 195 543 98 658
Fragments arranged by size:
1591 1352 658 543 195 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1107 1576 1645 1656 2571 2842 3871
Size: 406 701 469 69 11 915 271 1029
Cuts at: 3871 4437
Size: 566
Fragments arranged by size:
1029 915 701 566 469 406 271 69
11
BsiHKAI G_wGCw'C
Cuts at: 0 1237 1826 2755 2984 3416 4437
Size: 1237 589 929 229 432 1021
Fragments arranged by size:
1237 1021 929 589 432 229
BsmI GAATG_Cn'
Cuts at: 0 241 1507 1586 4318 4437
Size: 241 1266 79 2732 119
Fragments arranged by size:
2732 1266 241 119 79
Bsp1286I G_dGCh'C
Cuts at: 0 22 173 755 1237 1655 1826 2369 2619
Size: 22 151 582 482 418 171 543 250
Cuts at: 2619 2755 2899 2984 3392 3416 3688 4437
Size: 136 144 85 408 24 272 749
Fragments arranged by size:
749 582 543 482 418 408 272 250
171 151 144 136 85 24 22
BspEI T'CCGG_A
Cuts at: 0 3317 4437
Size: 3317 1120
BspHI T'CATG_A
Cuts at: 0 2579 2678 4437
Size: 2579 99 1759
Fragments arranged by size:
2579 1759 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 805 1756 2258 4317 4342 4437
Size: 428 377 951 502 2059 25 95
Fragments arranged by size:
2059 951 502 428 377 95 25
BsrBI CCG'CTC
Cuts at: 0 2612 3423 4437
Size: 2612 811 1014
Fragments arranged by size:
2612 1014 811
BsrDI GCAATG_nn'
Cuts at: 0 268 2044 2990 3269 4437
Size: 268 1776 946 279 1168
Fragments arranged by size:
1776 1168 946 279 268
BsrFI r'CCGG_y
Cuts at: 0 1427 2177 3245 3677 4437
Size: 1427 750 1068 432 760
Fragments arranged by size:
1427 1068 760 750 432
BsrGI T'GTAC_A
Cuts at: 0 350 2067 2357 4437
Size: 350 1717 290 2080
Fragments arranged by size:
2080 1717 350 290
BssSI C'ACGA_G
Cuts at: 0 46 3649 4437
Size: 46 3603 788
Fragments arranged by size:
3603 788 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 2549 4437
Size: 156 2393 1888
Fragments arranged by size:
2393 1888 156
BstEII G'GTnAC_C
Cuts at: 0 990 1132 4329 4437
Size: 990 142 3197 108
Fragments arranged by size:
3197 990 142 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2499 4437
Size: 2499 1938
BstYI r'GATC_y
Cuts at: 0 201 227 362 1115 1860 1995 3074 3110
Size: 201 26 135 753 745 135 1079 36
Cuts at: 3110 3497 3587 3815 3908 4437
Size: 387 90 228 93 529
Fragments arranged by size:
1079 753 745 529 387 228 201 135
135 93 90 36 26
Bsu36I CC'TnA_GG
Cuts at: 0 997 1316 4437
Size: 997 319 3121
Fragments arranged by size:
3121 997 319
BtgI C'CryG_G
Cuts at: 0 77 176 1111 1159 1346 1421 2492 2695
Size: 77 99 935 48 187 75 1071 203
Cuts at: 2695 2796 2867 2996 3150 3200 3434 3492 4057
Size: 101 71 129 154 50 234 58 565
Cuts at: 4057 4437
Size: 380
Fragments arranged by size:
1071 935 565 380 234 203 187 154
129 101 99 77 75 71 58 50
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1374 2133 4437
Size: 1374 759 2304
Fragments arranged by size:
2304 1374 759
BtsI GCAGTG_nn'
Cuts at: 0 582 2464 2535 3235 4437
Size: 582 1882 71 700 1202
Fragments arranged by size:
1882 1202 700 582 71
ClaI AT'CG_AT
Cuts at: 0 3341 4437
Size: 3341 1096
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 944 979 4437
Size: 944 35 3458
Fragments arranged by size:
3458 944 35
DraI TTT'AAA
Cuts at: 0 335 4437
Size: 335 4102
DraIII CAC_nnn'GTG
Cuts at: 0 2873 2969 3447 4241 4283 4437
Size: 2873 96 478 794 42 154
Fragments arranged by size:
2873 794 478 154 96 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 3520 4327 4437
Size: 3520 807 110
Fragments arranged by size:
3520 807 110
EaeI y'GGCC_r
Cuts at: 0 2 74 656 965 2786 2915 3216 3464
Size: 2 72 582 309 1821 129 301 248
Cuts at: 3464 3809 4437
Size: 345 628
Fragments arranged by size:
1821 628 582 345 309 301 248 129
72 2
EarI CTCTTCn'nnn_
Cuts at: 0 547 710 1298 4411 4437
Size: 547 163 588 3113 26
Fragments arranged by size:
3113 588 547 163 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 625 3889 4437
Size: 625 3264 548
Fragments arranged by size:
3264 625 548
EcoICRI GAG'CTC
Cuts at: 0 3414 4437
Size: 3414 1023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 998 1102 1745 2625 3192 4101 4137
Size: 181 817 104 643 880 567 909 36
Cuts at: 4137 4437
Size: 300
Fragments arranged by size:
909 880 817 643 567 300 181 104
36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2509 3118 4437
Size: 2509 609 1319
Fragments arranged by size:
2509 1319 609
EcoO109I rG'GnC_Cy
Cuts at: 0 18 19 734 899 2615 3558 3660 3685
Size: 18 1 715 165 1716 943 102 25
Cuts at: 3685 4391 4437
Size: 706 46
Fragments arranged by size:
1716 943 715 706 165 102 46 25
18 1
HaeII r_GCGC'y
Cuts at: 0 12 30 46 184 1094 1619 2273 2295
Size: 12 18 16 138 910 525 654 22
Cuts at: 2295 3649 3726 4437
Size: 1354 77 711
Fragments arranged by size:
1354 910 711 654 525 138 77 22
18 16 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 519 551 757 789 2110 2142
Size: 352 32 135 32 206 32 1321 32
Cuts at: 2142 2473 2505 4437
Size: 331 32 1932
Fragments arranged by size:
1932 1321 352 331 206 135 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1155 1515 1905 2088 3231 4437
Size: 1155 360 390 183 1143 1206
Fragments arranged by size:
1206 1155 1143 390 360 183
HindIII A'AGCT_T
Cuts at: 0 3299 4346 4437
Size: 3299 1047 91
Fragments arranged by size:
3299 1047 91
KasI G'GCGC_C
Cuts at: 0 26 180 1615 2269 4437
Size: 26 154 1435 654 2168
Fragments arranged by size:
2168 1435 654 154 26
MfeI C'AATT_G
Cuts at: 0 371 4437
Size: 371 4066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 2549 2762 4437
Size: 255 2294 213 1675
Fragments arranged by size:
2294 1675 255 213
MscI TGG'CCA
Cuts at: 0 4 2788 3218 3811 4437
Size: 4 2784 430 593 626
Fragments arranged by size:
2784 626 593 430 4
MslI CAynn'nnrTG
Cuts at: 0 136 1573 1603 2446 2452 2497 3694 3847
Size: 136 1437 30 843 6 45 1197 153
Cuts at: 3847 4437
Size: 590
Fragments arranged by size:
1437 1197 843 590 153 136 45 30
6
MspA1I CmG'CkG
Cuts at: 0 32 116 655 1113 1161 1220 2621 2660
Size: 32 84 539 458 48 59 1401 39
Cuts at: 2660 2697 2798 2826 2841 2943 3202 3399 3480
Size: 37 101 28 15 102 259 197 81
Cuts at: 3480 3783 3899 4437
Size: 303 116 538
Fragments arranged by size:
1401 539 538 458 303 259 197 116
102 101 84 81 59 48 39 37
32 28 15
NaeI GCC'GGC
Cuts at: 0 3679 4437
Size: 3679 758
NarI GG'CG_CC
Cuts at: 0 27 181 1616 2270 4437
Size: 27 154 1435 654 2167
Fragments arranged by size:
2167 1435 654 154 27
NcoI C'CATG_G
Cuts at: 0 3434 4437
Size: 3434 1003
NgoMIV G'CCGG_C
Cuts at: 0 3677 4437
Size: 3677 760
NsiI A_TGCA'T
Cuts at: 0 152 4137 4299 4437
Size: 152 3985 162 138
Fragments arranged by size:
3985 162 152 138
NspI r_CATG'y
Cuts at: 0 150 2068 2205 2451 2628 2880 2961 4301
Size: 150 1918 137 246 177 252 81 1340
Cuts at: 4301 4437
Size: 136
Fragments arranged by size:
1918 1340 252 246 177 150 137 136
81
PasI CC'CwG_GG
Cuts at: 0 1644 3760 3914 4387 4437
Size: 1644 2116 154 473 50
Fragments arranged by size:
2116 1644 473 154 50
PciI A'CATG_T
Cuts at: 0 2201 4437
Size: 2201 2236
PflMI CCAn_nnn'nTGG
Cuts at: 0 951 1347 1670 2472 4437
Size: 951 396 323 802 1965
Fragments arranged by size:
1965 951 802 396 323
PfoI T'CCnGG_A
Cuts at: 0 1120 2471 2830 3590 4437
Size: 1120 1351 359 760 847
Fragments arranged by size:
1351 1120 847 760 359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2179 2211 3412 3444 4148 4180 4437
Size: 2179 32 1201 32 704 32 257
Fragments arranged by size:
2179 1201 704 257 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 899 3558 3660 4437
Size: 899 2659 102 777
Fragments arranged by size:
2659 899 777 102
PshAI GACnn'nnGTC
Cuts at: 0 1766 1847 1946 4437
Size: 1766 81 99 2491
Fragments arranged by size:
2491 1766 99 81
PspOMI G'GGCC_C
Cuts at: 0 18 2615 3684 4437
Size: 18 2597 1069 753
Fragments arranged by size:
2597 1069 753 18
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 772 804 2674 2706 4437
Size: 772 32 1870 32 1731
Fragments arranged by size:
1870 1731 772 32 32
PstI C_TGCA'G
Cuts at: 0 802 1761 2255 2542 4437
Size: 802 959 494 287 1895
Fragments arranged by size:
1895 959 802 494 287
PvuII CAG'CTG
Cuts at: 0 2943 3399 3783 3899 4437
Size: 2943 456 384 116 538
Fragments arranged by size:
2943 538 456 384 116
RsrII CG'GwC_CG
Cuts at: 0 2828 4437
Size: 2828 1609
SacI G_AGCT'C
Cuts at: 0 3416 4437
Size: 3416 1021
SacII CC_GC'GG
Cuts at: 0 1114 1162 2698 2799 3203 4437
Size: 1114 48 1536 101 404 1234
Fragments arranged by size:
1536 1234 1114 404 101 48
SalI G'TCGA_C
Cuts at: 0 2086 4437
Size: 2086 2351
SanDI GG'GwC_CC
Cuts at: 0 899 4437
Size: 899 3538
SapI GCTCTTCn'nnn_
Cuts at: 0 710 1298 4437
Size: 710 588 3139
Fragments arranged by size:
3139 710 588
SexAI A'CCwGG_T
Cuts at: 0 1436 2120 3662 4437
Size: 1436 684 1542 775
Fragments arranged by size:
1542 1436 775 684
SfcI C'TryA_G
Cuts at: 0 798 1757 2251 2538 3516 3954 4437
Size: 798 959 494 287 978 438 483
Fragments arranged by size:
978 959 798 494 483 438 287
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 82 4437
Size: 82 4355
SfoI GGC'GCC
Cuts at: 0 28 182 1617 2271 4437
Size: 28 154 1435 654 2166
Fragments arranged by size:
2166 1435 654 154 28
SgrAI Cr'CCGG_yG
Cuts at: 0 2177 4437
Size: 2177 2260
SmaI CCC'GGG
Cuts at: 0 24 2602 3847 4437
Size: 24 2578 1245 590
Fragments arranged by size:
2578 1245 590 24
SmlI C'TyrA_G
Cuts at: 0 478 3007 4012 4437
Size: 478 2529 1005 425
Fragments arranged by size:
2529 1005 478 425
SphI G_CATG'C
Cuts at: 0 150 2880 2961 4301 4437
Size: 150 2730 81 1340 136
Fragments arranged by size:
2730 1340 150 136 81
StyI C'CwwG_G
Cuts at: 0 1367 1400 3434 4437
Size: 1367 33 2034 1003
Fragments arranged by size:
2034 1367 1003 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2458 3055 4437
Size: 2458 597 1382
Fragments arranged by size:
2458 1382 597
TatI w'GTAC_w
Cuts at: 0 350 788 2067 2357 4437
Size: 350 438 1279 290 2080
Fragments arranged by size:
2080 1279 438 350 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 291 323 680 712 1116 1148 1342 1374
Size: 291 32 357 32 404 32 194 32
Cuts at: 1374 3369 3401 3730 3762 4437
Size: 1995 32 329 32 675
Fragments arranged by size:
1995 675 404 357 329 291 194 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1258 1324 4437
Size: 1258 66 3113
Fragments arranged by size:
3113 1258 66
XbaI T'CTAG_A
Cuts at: 0 405 4437
Size: 405 4032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1667 2385 2940 3060 4242 4437
Size: 1667 718 555 120 1182 195
Fragments arranged by size:
1667 1182 718 555 195 120
XhoI C'TCGA_G
Cuts at: 0 478 4437
Size: 478 3959
XmaI C'CCGG_G
Cuts at: 0 22 2600 3845 4437
Size: 22 2578 1245 592
Fragments arranged by size:
2578 1245 592 22
XmnI GAAnn'nnTTC
Cuts at: 0 2320 4437
Size: 2320 2117
ZraI GAC'GTC
Cuts at: 0 1845 3803 4437
Size: 1845 1958 634
Fragments arranged by size:
1958 1845 634
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII HindIII KasI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuII RsrII SacI SacII SalI SanDI SapI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AgeI ApaLI AscI AseI AsiSI AvrII
BclI BmtI BplI BsaAI BsaBI BseRI BsiEI BsiWI
BsmBI BssHII BstBI BstZ17I EagI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MluI NdeI NheI
NotI NruI PacI PmeI PmlI PsiI PspXI PvuI
SbfI ScaI SgfI SnaBI SpeI SrfI SspI StuI
SwaI