Restriction map for
pF1KA0232
(Linear) (Six-base) MAPSORT of: KIAA0232.seq Check: 6467 from: 1 to: 4185
KIAA0232.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1886 4185
Size: 1886 2299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 857 1163 2596 2612 3200 4185
Size: 406 451 306 1433 16 588 985
Fragments arranged by size:
1433 985 588 451 406 306 16
AflII C'TTAA_G
Cuts at: 0 3129 4185
Size: 3129 1056
AflIII A'CryG_T
Cuts at: 0 2158 4185
Size: 2158 2027
AleI CACnn'nnGTG
Cuts at: 0 724 4185
Size: 724 3461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1668 4185
Size: 1668 2517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2118 2150 4185
Size: 2118 32 2035
Fragments arranged by size:
2118 2035 32
AlwNI CAG_nnn'CTG
Cuts at: 0 70 2890 4185
Size: 70 2820 1295
Fragments arranged by size:
2820 1295 70
ApaI G_GGCC'C
Cuts at: 0 4150 4185
Size: 4150 35
ApoI r'AATT_y
Cuts at: 0 772 2409 2505 3373 3520 4185
Size: 772 1637 96 868 147 665
Fragments arranged by size:
1637 868 772 665 147 96
AseI AT'TA_AT
Cuts at: 0 1913 2225 3719 4185
Size: 1913 312 1494 466
Fragments arranged by size:
1913 1494 466 312
AvaI C'yCGr_G
Cuts at: 0 139 4185
Size: 139 4046
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2903 2936 4185
Size: 2903 33 1249
Fragments arranged by size:
2903 1249 33
BamHI G'GATC_C
Cuts at: 0 1557 4185
Size: 1557 2628
BanI G'GyrC_C
Cuts at: 0 1564 4185
Size: 1564 2621
BanII G_rGCy'C
Cuts at: 0 140 401 1199 1901 4150 4166 4185
Size: 140 261 798 702 2249 16 19
Fragments arranged by size:
2249 798 702 261 140 19 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 181 340 857 3062 4004 4185
Size: 181 159 517 2205 942 181
Fragments arranged by size:
2205 942 517 181 181 159
BbvCI CC'TCA_GC
Cuts at: 0 1727 4185
Size: 1727 2458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1201 1235 4185
Size: 1201 34 2950
Fragments arranged by size:
2950 1201 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1278 4185
Size: 1278 2907
BclI T'GATC_A
Cuts at: 0 1542 2997 4185
Size: 1542 1455 1188
Fragments arranged by size:
1542 1455 1188
BglII A'GATC_T
Cuts at: 0 303 3447 4185
Size: 303 3144 738
Fragments arranged by size:
3144 738 303
Bme1580I G_kGCm'C
Cuts at: 0 2957 4150 4185
Size: 2957 1193 35
Fragments arranged by size:
2957 1193 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 1460 3197 3605 3968 4185
Size: 1460 1737 408 363 217
Fragments arranged by size:
1737 1460 408 363 217
BmtI G_CTAG'C
Cuts at: 0 647 4185
Size: 647 3538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 4137 4185
Size: 420 3717 48
Fragments arranged by size:
3717 420 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 458 479 858 1814 2623 2698 4073
Size: 390 68 21 379 956 809 75 1375
Cuts at: 4073 4074 4185
Size: 1 111
Fragments arranged by size:
1375 956 809 390 379 111 75 68
21 1
Bpu10I CC'TnA_GC
Cuts at: 0 1727 2463 4185
Size: 1727 736 1722
Fragments arranged by size:
1727 1722 736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 873 3401 3529 4185
Size: 36 837 2528 128 656
Fragments arranged by size:
2528 837 656 128 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 470 1095 1255 2599 4185
Size: 470 625 160 1344 1586
Fragments arranged by size:
1586 1344 625 470 160
BsaBI GATnn'nnATC
Cuts at: 0 1502 1541 4185
Size: 1502 39 2644
Fragments arranged by size:
2644 1502 39
BsaWI w'CCGG_w
Cuts at: 0 1792 4185
Size: 1792 2393
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 645 675 1186 1216 4185
Size: 645 30 511 30 2969
Fragments arranged by size:
2969 645 511 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 409 1207 1835 2807 3299 3371 3872
Size: 39 370 798 628 972 492 72 501
Cuts at: 3872 4174 4185
Size: 302 11
Fragments arranged by size:
972 798 628 501 492 370 302 72
39 11
BseYI C'CCAG_C
Cuts at: 0 126 251 4185
Size: 126 125 3934
Fragments arranged by size:
3934 126 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 2903 4185
Size: 136 2767 1282
Fragments arranged by size:
2767 1282 136
BsiHKAI G_wGCw'C
Cuts at: 0 140 401 1141 1868 1901 3564 4090 4166
Size: 140 261 740 727 33 1663 526 76
Cuts at: 4166 4185
Size: 19
Fragments arranged by size:
1663 740 727 526 261 140 76 33
19
BsmI GAATG_Cn'
Cuts at: 0 3169 4185
Size: 3169 1016
Bsp1286I G_dGCh'C
Cuts at: 0 140 401 1141 1199 1868 1901 2957 3564
Size: 140 261 740 58 669 33 1056 607
Cuts at: 3564 4090 4150 4166 4185
Size: 526 60 16 19
Fragments arranged by size:
1056 740 669 607 526 261 140 60
58 33 19 16
BspHI T'CATG_A
Cuts at: 0 572 4185
Size: 572 3613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 1375 1886 3733 4185
Size: 227 1148 511 1847 452
Fragments arranged by size:
1847 1148 511 452 227
BsrDI GCAATG_nn'
Cuts at: 0 1966 4185
Size: 1966 2219
BsrGI T'GTAC_A
Cuts at: 0 13 809 1157 4033 4185
Size: 13 796 348 2876 152
Fragments arranged by size:
2876 796 348 152 13
BssSI C'ACGA_G
Cuts at: 0 858 956 4185
Size: 858 98 3229
Fragments arranged by size:
3229 858 98
BstBI TT'CG_AA
Cuts at: 0 966 4185
Size: 966 3219
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3493 4185
Size: 3493 692
BstYI r'GATC_y
Cuts at: 0 303 1128 1557 1921 3093 3302 3435 3447
Size: 303 825 429 364 1172 209 133 12
Cuts at: 3447 4185
Size: 738
Fragments arranged by size:
1172 825 738 429 364 303 209 133
12
Bsu36I CC'TnA_GG
Cuts at: 0 665 4185
Size: 665 3520
BtgI C'CryG_G
Cuts at: 0 2137 4074 4185
Size: 2137 1937 111
Fragments arranged by size:
2137 1937 111
BtsI GCAGTG_nn'
Cuts at: 0 714 1071 1316 4185
Size: 714 357 245 2869
Fragments arranged by size:
2869 714 357 245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2492 2527 4185
Size: 2492 35 1658
Fragments arranged by size:
2492 1658 35
DraI TTT'AAA
Cuts at: 0 1109 3210 4185
Size: 1109 2101 975
Fragments arranged by size:
2101 1109 975
EarI CTCTTCn'nnn_
Cuts at: 0 431 621 3001 3791 3977 4106 4151 4185
Size: 431 190 2380 790 186 129 45 34
Fragments arranged by size:
2380 790 431 190 186 129 45 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 996 4145 4185
Size: 996 3149 40
Fragments arranged by size:
3149 996 40
EcoICRI GAG'CTC
Cuts at: 0 138 399 1899 4164 4185
Size: 138 261 1500 2265 21
Fragments arranged by size:
2265 1500 261 138 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 406 458 479 857 858 1163 1814
Size: 390 16 52 21 378 1 305 651
Cuts at: 1814 2596 2612 2623 2698 3200 4073 4074 4185
Size: 782 16 11 75 502 873 1 111
Fragments arranged by size:
873 782 651 502 390 378 305 111
75 52 21 16 16 11 1 1
EcoO109I rG'GnC_Cy
Cuts at: 0 67 1454 1746 4147 4185
Size: 67 1387 292 2401 38
Fragments arranged by size:
2401 1387 292 67 38
HaeII r_GCGC'y
Cuts at: 0 3990 4097 4185
Size: 3990 107 88
Fragments arranged by size:
3990 107 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 122 154 156 188 355 387 643 675
Size: 122 32 2 32 167 32 256 32
Cuts at: 675 837 869 1113 1145 1285 1317 3294 3326
Size: 162 32 244 32 140 32 1977 32
Cuts at: 3326 3496 3528 4185
Size: 170 32 657
Fragments arranged by size:
1977 657 256 244 170 167 162 140
122 32 32 32 32 32 32 32
32 32 2
HincII GTy'rAC
Cuts at: 0 3276 4185
Size: 3276 909
MfeI C'AATT_G
Cuts at: 0 788 1217 4185
Size: 788 429 2968
Fragments arranged by size:
2968 788 429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 1535 1573 4185
Size: 849 686 38 2612
Fragments arranged by size:
2612 849 686 38
MslI CAynn'nnrTG
Cuts at: 0 577 724 1526 2427 2450 3491 3900 4185
Size: 577 147 802 901 23 1041 409 285
Fragments arranged by size:
1041 901 802 577 409 285 147 23
MspA1I CmG'CkG
Cuts at: 0 1083 3792 4076 4185
Size: 1083 2709 284 109
Fragments arranged by size:
2709 1083 284 109
NcoI C'CATG_G
Cuts at: 0 2137 4185
Size: 2137 2048
NheI G'CTAG_C
Cuts at: 0 643 4185
Size: 643 3542
PasI CC'CwG_GG
Cuts at: 0 2750 4185
Size: 2750 1435
PfoI T'CCnGG_A
Cuts at: 0 3096 4185
Size: 3096 1089
PpuMI rG'GwC_Cy
Cuts at: 0 1454 1746 4185
Size: 1454 292 2439
Fragments arranged by size:
2439 1454 292
PsiI TTA'TAA
Cuts at: 0 269 1429 4185
Size: 269 1160 2756
Fragments arranged by size:
2756 1160 269
PspOMI G'GGCC_C
Cuts at: 0 4146 4185
Size: 4146 39
PstI C_TGCA'G
Cuts at: 0 224 1425 1745 4185
Size: 224 1201 320 2440
Fragments arranged by size:
2440 1201 320 224
PvuII CAG'CTG
Cuts at: 0 1083 3792 4185
Size: 1083 2709 393
Fragments arranged by size:
2709 1083 393
SacI G_AGCT'C
Cuts at: 0 140 401 1901 4166 4185
Size: 140 261 1500 2265 19
Fragments arranged by size:
2265 1500 261 140 19
SacII CC_GC'GG
Cuts at: 0 4077 4185
Size: 4077 108
SapI GCTCTTCn'nnn_
Cuts at: 0 621 3977 4185
Size: 621 3356 208
Fragments arranged by size:
3356 621 208
SbfI CC_TGCA'GG
Cuts at: 0 224 1745 4185
Size: 224 1521 2440
Fragments arranged by size:
2440 1521 224
SexAI A'CCwGG_T
Cuts at: 0 3856 4185
Size: 3856 329
SfcI C'TryA_G
Cuts at: 0 220 1421 1741 2457 2516 4185
Size: 220 1201 320 716 59 1669
Fragments arranged by size:
1669 1201 716 320 220 59
SmlI C'TyrA_G
Cuts at: 0 51 139 888 3129 3416 3508 4185
Size: 51 88 749 2241 287 92 677
Fragments arranged by size:
2241 749 677 287 92 88 51
SspI AAT'ATT
Cuts at: 0 1228 2184 3402 4185
Size: 1228 956 1218 783
Fragments arranged by size:
1228 1218 956 783
StyI C'CwwG_G
Cuts at: 0 695 1749 2137 3926 4185
Size: 695 1054 388 1789 259
Fragments arranged by size:
1789 1054 695 388 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3987 4131 4185
Size: 3987 144 54
Fragments arranged by size:
3987 144 54
TatI w'GTAC_w
Cuts at: 0 13 518 809 1157 3875 4033 4185
Size: 13 505 291 348 2718 158 152
Fragments arranged by size:
2718 505 348 291 158 152 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 3255 3287 4185
Size: 1186 32 2037 32 898
Fragments arranged by size:
2037 1186 898 32 32
XbaI T'CTAG_A
Cuts at: 0 312 2056 2274 2583 4185
Size: 312 1744 218 309 1602
Fragments arranged by size:
1744 1602 312 309 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 119 4185
Size: 119 4066
XhoI C'TCGA_G
Cuts at: 0 139 4185
Size: 139 4046
XmnI GAAnn'nnTTC
Cuts at: 0 530 2270 2974 3251 4185
Size: 530 1740 704 277 934
Fragments arranged by size:
1740 934 704 530 277
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlfI AloI AlwNI
ApaI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglII Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstBI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MfeI MmeI MslI MspA1I
NcoI NheI PasI PfoI PpuMI PsiI PspOMI PstI
PvuII SacI SacII SapI SbfI SexAI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI ApaLI
AscI AsiSI AvrII BbeI BfrBI BglI BlpI BmgBI
BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BstAPI BstEII BstZ17I BtgZI ClaI DraIII DrdI
EaeI EagI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MluI MscI NaeI
NarI NdeI NgoMIV NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SalI SanDI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XmaI ZraI