Restriction map for
pF1KA0233
(Linear) (Six-base) MAPSORT of: KIAA0233.seq Check: 4646 from: 1 to: 7563
KIAA0233.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2307 2397 2804 3330 7563
Size: 2307 90 407 526 4233
Fragments arranged by size:
4233 2307 526 407 90
AatII G_ACGT'C
Cuts at: 0 2393 5879 7563
Size: 2393 3486 1684
Fragments arranged by size:
3486 2393 1684
AccI GT'mk_AC
Cuts at: 0 98 665 1796 1808 1850 1931 5610 5909
Size: 98 567 1131 12 42 81 3679 299
Cuts at: 5909 7563
Size: 1654
Fragments arranged by size:
3679 1654 1131 567 299 98 81 42
12
Acc65I G'GTAC_C
Cuts at: 0 5997 7563
Size: 5997 1566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 355 717 1636 2345 2500 3493 3744
Size: 63 292 362 919 709 155 993 251
Cuts at: 3744 4319 7359 7563
Size: 575 3040 204
Fragments arranged by size:
3040 993 919 709 575 362 292 251
204 155 63
AfeI AGC'GCT
Cuts at: 0 4639 5182 7563
Size: 4639 543 2381
Fragments arranged by size:
4639 2381 543
AflIII A'CryG_T
Cuts at: 0 1445 1811 2138 2400 7331 7563
Size: 1445 366 327 262 4931 232
Fragments arranged by size:
4931 1445 366 327 262 232
AhdI GACnn_n'nnGTC
Cuts at: 0 5973 7563
Size: 5973 1590
AleI CACnn'nnGTG
Cuts at: 0 2317 7563
Size: 2317 5246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2071 2701 3622 6094 7267 7378 7563
Size: 2071 630 921 2472 1173 111 185
Fragments arranged by size:
2472 2071 1173 921 630 185 111
AlwNI CAG_nnn'CTG
Cuts at: 0 270 290 743 819 1689 1701 2654 2712
Size: 270 20 453 76 870 12 953 58
Cuts at: 2712 2922 2934 3159 3869 4539 4920 5400 6753
Size: 210 12 225 710 670 381 480 1353
Cuts at: 6753 7563
Size: 810
Fragments arranged by size:
1353 953 870 810 710 670 480 453
381 270 225 210 76 58 20 12
12
ApaI G_GGCC'C
Cuts at: 0 1492 2729 3279 3630 4138 4166 4477 5641
Size: 1492 1237 550 351 508 28 311 1164
Cuts at: 5641 7097 7563
Size: 1456 466
Fragments arranged by size:
1492 1456 1237 1164 550 508 466 351
311 28
ApaLI G'TGCA_C
Cuts at: 0 1153 4678 7563
Size: 1153 3525 2885
Fragments arranged by size:
3525 2885 1153
ApoI r'AATT_y
Cuts at: 0 2977 7563
Size: 2977 4586
AvaI C'yCGr_G
Cuts at: 0 725 1049 1216 4305 4477 4882 5166 6001
Size: 725 324 167 3089 172 405 284 835
Cuts at: 6001 7024 7469 7563
Size: 1023 445 94
Fragments arranged by size:
3089 1023 835 725 445 405 324 284
172 167 94
BanI G'GyrC_C
Cuts at: 0 419 766 1113 1549 2055 2319 2840 2908
Size: 419 347 347 436 506 264 521 68
Cuts at: 2908 3086 4601 4609 4900 4955 5496 5997 6333
Size: 178 1515 8 291 55 541 501 336
Cuts at: 6333 7030 7563
Size: 697 533
Fragments arranged by size:
1515 697 541 533 521 506 501 436
419 347 347 336 291 264 178 68
55 8
BanII G_rGCy'C
Cuts at: 0 448 1177 1217 1492 1673 2039 2175 2666
Size: 448 729 40 275 181 366 136 491
Cuts at: 2666 2729 3279 3425 3630 4138 4166 4183 4477
Size: 63 550 146 205 508 28 17 294
Cuts at: 4477 4661 5582 5641 6875 7097 7563
Size: 184 921 59 1234 222 466
Fragments arranged by size:
1234 921 729 550 508 491 466 448
366 294 275 222 205 184 181 146
136 63 59 40 28 17
BbeI G_GCGC'C
Cuts at: 0 423 770 2844 4959 7563
Size: 423 347 2074 2115 2604
Fragments arranged by size:
2604 2115 2074 423 347
BbsI GAAGACnn'nnnn_
Cuts at: 0 3477 3705 3894 4307 5133 5196 5338 5522
Size: 3477 228 189 413 826 63 142 184
Cuts at: 5522 7563
Size: 2041
Fragments arranged by size:
3477 2041 826 413 228 189 184 142
63
BbvCI CC'TCA_GC
Cuts at: 0 196 4375 5672 7563
Size: 196 4179 1297 1891
Fragments arranged by size:
4179 1891 1297 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1746 1780 5863 5897 6201 6235 7563
Size: 1746 34 4083 34 304 34 1328
Fragments arranged by size:
4083 1746 1328 304 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4078 7168 7563
Size: 4078 3090 395
Fragments arranged by size:
4078 3090 395
BclI T'GATC_A
Cuts at: 0 7538 7563
Size: 7538 25
BglI GCCn_nnn'nGGC
Cuts at: 0 1013 1197 4197 5826 6489 7563
Size: 1013 184 3000 1629 663 1074
Fragments arranged by size:
3000 1629 1074 1013 663 184
BglII A'GATC_T
Cuts at: 0 233 2990 7382 7563
Size: 233 2757 4392 181
Fragments arranged by size:
4392 2757 233 181
BlpI GC'TnA_GC
Cuts at: 0 1211 2354 3353 4522 7563
Size: 1211 1143 999 1169 3041
Fragments arranged by size:
3041 1211 1169 1143 999
Bme1580I G_kGCm'C
Cuts at: 0 254 1118 1157 1492 2060 2729 3279 3437
Size: 254 864 39 335 568 669 550 158
Cuts at: 3437 3630 4138 4166 4477 4682 5641 5812 6858
Size: 193 508 28 311 205 959 171 1046
Cuts at: 6858 7033 7086 7097 7563
Size: 175 53 11 466
Fragments arranged by size:
1046 959 864 669 568 550 508 466
335 311 254 205 193 175 171 158
53 39 28 11
BmgBI CAC'GTC
Cuts at: 0 3927 4623 5975 7563
Size: 3927 696 1352 1588
Fragments arranged by size:
3927 1588 1352 696
BmrI ACTGGGnnnn_n'
Cuts at: 0 6378 7563
Size: 6378 1185
BmtI G_CTAG'C
Cuts at: 0 1066 3961 7563
Size: 1066 2895 3602
Fragments arranged by size:
3602 2895 1066
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 318 2058 2286 5448 6834 7050 7563
Size: 318 1740 228 3162 1386 216 513
Fragments arranged by size:
3162 1740 1386 513 318 228 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 642 873 1078 1537 1616 2028 2035
Size: 214 428 231 205 459 79 412 7
Cuts at: 2035 2256 2386 2449 3007 3231 3424 3925 3948
Size: 221 130 63 558 224 193 501 23
Cuts at: 3948 4174 4996 5347 5768 7498 7504 7563
Size: 226 822 351 421 1730 6 59
Fragments arranged by size:
1730 822 558 501 459 428 421 412
351 231 226 224 221 214 205 193
130 79 63 59 23 7 6
Bpu10I CC'TnA_GC
Cuts at: 0 196 1469 3907 4375 4831 5581 5672 7563
Size: 196 1273 2438 468 456 750 91 1891
Fragments arranged by size:
2438 1891 1273 750 468 456 196 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1861 2605 2932 3982 6625 7417 7563
Size: 844 1017 744 327 1050 2643 792 146
Fragments arranged by size:
2643 1050 1017 844 792 744 327 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 396 862 3809 7525 7563
Size: 289 107 466 2947 3716 38
Fragments arranged by size:
3716 2947 466 289 107 38
BsaAI yAC'GTr
Cuts at: 0 12 1110 1446 2139 5271 7332 7563
Size: 12 1098 336 693 3132 2061 231
Fragments arranged by size:
3132 2061 1098 693 336 231 12
BsaHI Gr'CG_yC
Cuts at: 0 420 767 950 1225 2390 2841 3386 4956
Size: 420 347 183 275 1165 451 545 1570
Cuts at: 4956 5876 7563
Size: 920 1687
Fragments arranged by size:
1687 1570 1165 920 545 451 420 347
275 183
BsaWI w'CCGG_w
Cuts at: 0 7527 7563
Size: 7527 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 994 1024 2125 2155 4643 4673 5741 5771
Size: 994 30 1101 30 2488 30 1068 30
Cuts at: 5771 6932 6962 7399 7429 7477 7480 7507 7510
Size: 1161 30 437 30 48 3 27 3
Cuts at: 7510 7563
Size: 53
Fragments arranged by size:
2488 1161 1101 1068 994 437 53 48
30 30 30 30 30 30 27 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 384 2230 2263 2266 2269 2272 2275 2278
Size: 384 1846 33 3 3 3 3 3
Cuts at: 2278 3379 3856 4042 4173 4297 4300 4495 4636
Size: 1101 477 186 131 124 3 195 141
Cuts at: 4636 4804 4879 4993 5440 5479 5482 5497 6736
Size: 168 75 114 447 39 3 15 1239
Cuts at: 6736 7147 7420 7501 7504 7563
Size: 411 273 81 3 59
Fragments arranged by size:
1846 1239 1101 477 447 411 384 273
195 186 168 141 131 124 114 81
75 59 39 33 15 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 189 277 290 595 852 1509 1998 2168
Size: 189 88 13 305 257 657 489 170
Cuts at: 2168 2286 3329 3558 3743 4043 4447 4548 4779
Size: 118 1043 229 185 300 404 101 231
Cuts at: 4779 5173 5393 5640 6081 6188 6645 6680 6746
Size: 394 220 247 441 107 457 35 66
Cuts at: 6746 6847 7563
Size: 101 716
Fragments arranged by size:
1043 716 657 489 457 441 404 394
305 300 257 247 231 229 220 189
185 170 118 107 101 101 88 66
35 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 2077 2425 2640 3034 3907 4531 5383
Size: 675 1402 348 215 394 873 624 852
Cuts at: 5383 5833 6983 7563
Size: 450 1150 580
Fragments arranged by size:
1402 1150 873 852 675 624 580 450
394 348 215
BsiEI CG_ry'CG
Cuts at: 0 1785 3399 5768 5821 6890 7563
Size: 1785 1614 2369 53 1069 673
Fragments arranged by size:
2369 1785 1614 1069 673 53
BsiHKAI G_wGCw'C
Cuts at: 0 17 1157 1177 1399 1523 2039 2138 2852
Size: 17 1140 20 222 124 516 99 714
Cuts at: 2852 4528 4661 4682 4837 5138 5582 6875 7563
Size: 1676 133 21 155 301 444 1293 688
Fragments arranged by size:
1676 1293 1140 714 688 516 444 301
222 155 133 124 99 21 20 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1232 7563
Size: 1232 6331
Bsp1286I G_dGCh'C
Cuts at: 0 17 254 448 1118 1157 1177 1217 1399
Size: 17 237 194 670 39 20 40 182
Cuts at: 1399 1492 1523 1673 2039 2060 2138 2175 2666
Size: 93 31 150 366 21 78 37 491
Cuts at: 2666 2729 2852 3279 3425 3437 3630 4138 4166
Size: 63 123 427 146 12 193 508 28
Cuts at: 4166 4183 4477 4528 4661 4682 4837 5138 5582
Size: 17 294 51 133 21 155 301 444
Cuts at: 5582 5641 5812 6858 6875 7033 7086 7097 7563
Size: 59 171 1046 17 158 53 11 466
Fragments arranged by size:
1046 670 508 491 466 444 427 366
301 294 237 194 193 182 171 158
155 150 146 133 123 93 78 63
59 53 51 40 39 37 31 28
21 21 20 17 17 17 12 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 677 1469 2307 2397 2510 2593 2804
Size: 110 567 792 838 90 113 83 211
Cuts at: 2804 3167 3266 3330 3443 3575 3593 3929 6074
Size: 363 99 64 113 132 18 336 2145
Cuts at: 6074 7256 7563
Size: 1182 307
Fragments arranged by size:
2145 1182 838 792 567 363 336 307
211 132 113 113 110 99 90 83
64 18
BsrBI CCG'CTC
Cuts at: 0 556 1233 2358 5390 5459 5656 5744 7523
Size: 556 677 1125 3032 69 197 88 1779
Cuts at: 7523 7563
Size: 40
Fragments arranged by size:
3032 1779 1125 677 556 197 88 69
40
BsrDI GCAATG_nn'
Cuts at: 0 184 1283 3098 7563
Size: 184 1099 1815 4465
Fragments arranged by size:
4465 1815 1099 184
BsrFI r'CCGG_y
Cuts at: 0 769 832 916 1165 1780 2377 2837 2858
Size: 769 63 84 249 615 597 460 21
Cuts at: 2858 3733 4837 5113 7192 7563
Size: 875 1104 276 2079 371
Fragments arranged by size:
2079 1104 875 769 615 597 460 371
276 249 84 63 21
BsrGI T'GTAC_A
Cuts at: 0 2459 3659 4703 7563
Size: 2459 1200 1044 2860
Fragments arranged by size:
2860 2459 1200 1044
BssHII G'CGCG_C
Cuts at: 0 50 1277 7563
Size: 50 1227 6286
Fragments arranged by size:
6286 1227 50
BssSI C'ACGA_G
Cuts at: 0 1156 1789 1894 3634 7550 7563
Size: 1156 633 105 1740 3916 13
Fragments arranged by size:
3916 1740 1156 633 105 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 249 743 1113 2805 4324 4518 7563
Size: 249 494 370 1692 1519 194 3045
Fragments arranged by size:
3045 1692 1519 494 370 249 194
BstEII G'GTnAC_C
Cuts at: 0 1650 3042 4353 7563
Size: 1650 1392 1311 3210
Fragments arranged by size:
3210 1650 1392 1311
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 1485 2052 4779 7344 7563
Size: 360 1125 567 2727 2565 219
Fragments arranged by size:
2727 2565 1125 567 360 219
BstYI r'GATC_y
Cuts at: 0 233 1739 2931 2990 6239 7382 7563
Size: 233 1506 1192 59 3249 1143 181
Fragments arranged by size:
3249 1506 1192 1143 233 181 59
Bsu36I CC'TnA_GG
Cuts at: 0 6785 7563
Size: 6785 778
BtgI C'CryG_G
Cuts at: 0 454 749 974 1454 1655 2555 2693 2729
Size: 454 295 225 480 201 900 138 36
Cuts at: 2729 4192 4605 5549 5717 6041 6596 7256 7563
Size: 1463 413 944 168 324 555 660 307
Fragments arranged by size:
1463 944 900 660 555 480 454 413
324 307 295 225 201 168 138 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 621 1908 6534 7563
Size: 621 1287 4626 1029
Fragments arranged by size:
4626 1287 1029 621
BtsI GCAGTG_nn'
Cuts at: 0 616 2195 2203 3340 3447 4207 4860 4866
Size: 616 1579 8 1137 107 760 653 6
Cuts at: 4866 6365 6995 7563
Size: 1499 630 568
Fragments arranged by size:
1579 1499 1137 760 653 630 616 568
107 8 6
ClaI AT'CG_AT
Cuts at: 0 6623 7563
Size: 6623 940
DraIII CAC_nnn'GTG
Cuts at: 0 698 1113 2579 4324 5274 7563
Size: 698 415 1466 1745 950 2289
Fragments arranged by size:
2289 1745 1466 950 698 415
EaeI y'GGCC_r
Cuts at: 0 551 608 771 1014 1167 1385 1782 2294
Size: 551 57 163 243 153 218 397 512
Cuts at: 2294 3005 3068 3479 4931 5048 5195 5504 5765
Size: 711 63 411 1452 117 147 309 261
Cuts at: 5765 5818 5960 6116 6755 6887 7013 7563
Size: 53 142 156 639 132 126 550
Fragments arranged by size:
1452 711 639 551 550 512 411 397
309 261 243 218 163 156 153 147
142 132 126 117 63 57 53
EagI C'GGCC_G
Cuts at: 0 1782 5765 5818 6887 7563
Size: 1782 3983 53 1069 676
Fragments arranged by size:
3983 1782 1069 676 53
EarI CTCTTCn'nnn_
Cuts at: 0 112 211 682 1825 1846 2044 3133 3598
Size: 112 99 471 1143 21 198 1089 465
Cuts at: 3598 3763 4274 5684 5696 5725 5748 6313 6319
Size: 165 511 1410 12 29 23 565 6
Cuts at: 6319 6547 6580 7069 7563
Size: 228 33 489 494
Fragments arranged by size:
1410 1143 1089 565 511 494 489 471
465 228 198 165 112 99 33 29
23 21 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1881 5100 6219 7563
Size: 1881 3219 1119 1344
Fragments arranged by size:
3219 1881 1344 1119
EcoICRI GAG'CTC
Cuts at: 0 1175 2037 4659 5580 6873 7563
Size: 1175 862 2622 921 1293 690
Fragments arranged by size:
2622 1293 1175 921 862 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 214 355 642 717 873 1078 1537
Size: 63 151 141 287 75 156 205 459
Cuts at: 1537 1616 1636 2028 2035 2256 2345 2386 2449
Size: 79 20 392 7 221 89 41 63
Cuts at: 2449 2500 3007 3231 3424 3493 3744 3925 3948
Size: 51 507 224 193 69 251 181 23
Cuts at: 3948 4174 4319 4996 5347 5768 7359 7498 7504
Size: 226 145 677 351 421 1591 139 6
Cuts at: 7504 7563
Size: 59
Fragments arranged by size:
1591 677 507 459 421 392 351 287
251 226 224 221 205 193 181 156
151 145 141 139 89 79 75 69
63 63 59 51 41 23 20 7
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1843 2482 4651 5803 6316 6934 7024 7563
Size: 1843 639 2169 1152 513 618 90 539
Fragments arranged by size:
2169 1843 1152 639 618 539 513 90
EcoO109I rG'GnC_Cy
Cuts at: 0 274 603 874 1489 2004 2108 2198 2343
Size: 274 329 271 615 515 104 90 145
Cuts at: 2343 2725 3276 3455 4135 4143 4162 4473 4474
Size: 382 551 179 680 8 19 311 1
Cuts at: 4474 4735 4746 5558 5585 5637 5712 6927 7563
Size: 261 11 812 27 52 75 1215 636
Fragments arranged by size:
1215 812 680 636 615 551 515 382
329 311 274 271 261 179 145 104
90 75 52 27 19 11 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5319 6111 7527 7563
Size: 5319 792 1416 36
Fragments arranged by size:
5319 1416 792 36
FspI TGC'GCA
Cuts at: 0 988 1369 4963 6070 7563
Size: 988 381 3594 1107 1493
Fragments arranged by size:
3594 1493 1107 988 381
HaeII r_GCGC'y
Cuts at: 0 423 770 2844 3139 3210 3897 4561 4641
Size: 423 347 2074 295 71 687 664 80
Cuts at: 4641 4959 5017 5184 5286 5832 6109 6680 6829
Size: 318 58 167 102 546 277 571 149
Cuts at: 6829 7563
Size: 734
Fragments arranged by size:
2074 734 687 664 571 546 423 347
318 295 277 167 149 102 80 71
58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 435 467 2125 2157 2380 2412
Size: 137 32 266 32 1658 32 223 32
Cuts at: 2412 4018 4050 5422 5454 5959 5991 6712 6744
Size: 1606 32 1372 32 505 32 721 32
Cuts at: 6744 7563
Size: 819
Fragments arranged by size:
1658 1606 1372 819 721 505 266 223
137 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2631 3390 5910 6051 6612 7563
Size: 2631 759 2520 141 561 951
Fragments arranged by size:
2631 2520 951 759 561 141
KasI G'GCGC_C
Cuts at: 0 419 766 2840 4955 7563
Size: 419 347 2074 2115 2608
Fragments arranged by size:
2608 2115 2074 419 347
KpnI G_GTAC'C
Cuts at: 0 6001 7563
Size: 6001 1562
MluI A'CGCG_T
Cuts at: 0 2400 7563
Size: 2400 5163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4292 5523 7563
Size: 4292 1231 2040
Fragments arranged by size:
4292 2040 1231
MscI TGG'CCA
Cuts at: 0 1387 2296 3070 6118 6757 7015 7563
Size: 1387 909 774 3048 639 258 548
Fragments arranged by size:
3048 1387 909 774 639 548 258
MslI CAynn'nnrTG
Cuts at: 0 385 1288 2053 2128 2317 2596 5527 7563
Size: 385 903 765 75 189 279 2931 2036
Fragments arranged by size:
2931 2036 903 765 385 279 189 75
MspA1I CmG'CkG
Cuts at: 0 83 281 290 751 1319 1509 1636 1998
Size: 83 198 9 461 568 190 127 362
Cuts at: 1998 2091 2871 2880 2906 2922 3352 3411 4047
Size: 93 780 9 26 16 430 59 636
Cuts at: 4047 4335 4489 4701 5397 6245 6404 6570 6663
Size: 288 154 212 696 848 159 166 93
Cuts at: 6663 6753 7011 7119 7164 7563
Size: 90 258 108 45 399
Fragments arranged by size:
848 780 696 636 568 461 430 399
362 288 258 212 198 190 166 159
154 127 108 93 93 90 83 59
45 26 16 9 9
NaeI GCC'GGC
Cuts at: 0 771 834 1782 2379 2860 5115 7563
Size: 771 63 948 597 481 2255 2448
Fragments arranged by size:
2448 2255 948 771 597 481 63
NarI GG'CG_CC
Cuts at: 0 420 767 2841 4956 7563
Size: 420 347 2074 2115 2607
Fragments arranged by size:
2607 2115 2074 420 347
NcoI C'CATG_G
Cuts at: 0 1454 2555 6041 7256 7563
Size: 1454 1101 3486 1215 307
Fragments arranged by size:
3486 1454 1215 1101 307
NgoMIV G'CCGG_C
Cuts at: 0 769 832 1780 2377 2858 5113 7563
Size: 769 63 948 597 481 2255 2450
Fragments arranged by size:
2450 2255 948 769 597 481 63
NheI G'CTAG_C
Cuts at: 0 1062 3957 7563
Size: 1062 2895 3606
Fragments arranged by size:
3606 2895 1062
NruI TCG'CGA
Cuts at: 0 306 7563
Size: 306 7257
NspI r_CATG'y
Cuts at: 0 1761 1815 3471 3693 6975 7563
Size: 1761 54 1656 222 3282 588
Fragments arranged by size:
3282 1761 1656 588 222 54
PasI CC'CwG_GG
Cuts at: 0 1485 4131 5805 7563
Size: 1485 2646 1674 1758
Fragments arranged by size:
2646 1758 1674 1485
PciI A'CATG_T
Cuts at: 0 1811 7563
Size: 1811 5752
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 903 1110 1242 1383 1736 2579 2922
Size: 270 633 207 132 141 353 843 343
Cuts at: 2922 4458 5046 6180 6560 6753 7047 7563
Size: 1536 588 1134 380 193 294 516
Fragments arranged by size:
1536 1134 843 633 588 516 380 353
343 294 270 207 193 141 132
PfoI T'CCnGG_A
Cuts at: 0 1946 4946 7563
Size: 1946 3000 2617
Fragments arranged by size:
3000 2617 1946
PmlI CAC'GTG
Cuts at: 0 12 1110 2139 5271 7563
Size: 12 1098 1029 3132 2292
Fragments arranged by size:
3132 2292 1098 1029 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6151 6183 6649 6681 7563
Size: 6151 32 466 32 882
Fragments arranged by size:
6151 882 466 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 274 603 874 2004 2198 3455 4143 5558
Size: 274 329 271 1130 194 1257 688 1415
Cuts at: 5558 6927 7563
Size: 1369 636
Fragments arranged by size:
1415 1369 1257 1130 688 636 329 274
271 194
PshAI GACnn'nnGTC
Cuts at: 0 1648 2587 4112 7563
Size: 1648 939 1525 3451
Fragments arranged by size:
3451 1648 1525 939
PspOMI G'GGCC_C
Cuts at: 0 1488 2725 3275 3626 4134 4162 4473 5637
Size: 1488 1237 550 351 508 28 311 1164
Cuts at: 5637 7093 7563
Size: 1456 470
Fragments arranged by size:
1488 1456 1237 1164 550 508 470 351
311 28
PspXI vC'TCGA_Gb
Cuts at: 0 7024 7563
Size: 7024 539
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5284 5316 7563
Size: 5284 32 2247
Fragments arranged by size:
5284 2247 32
PstI C_TGCA'G
Cuts at: 0 286 512 2090 3454 3614 3893 4589 4667
Size: 286 226 1578 1364 160 279 696 78
Cuts at: 4667 5783 7124 7226 7563
Size: 1116 1341 102 337
Fragments arranged by size:
1578 1364 1341 1116 696 337 286 279
226 160 102 78
PvuII CAG'CTG
Cuts at: 0 281 1319 1509 1636 1998 2091 2871 2922
Size: 281 1038 190 127 362 93 780 51
Cuts at: 2922 3352 4047 4335 4701 5397 6404 7011 7119
Size: 430 695 288 366 696 1007 607 108
Cuts at: 7119 7164 7563
Size: 45 399
Fragments arranged by size:
1038 1007 780 696 695 607 430 399
366 362 288 281 190 127 108 93
51 45
RsrII CG'GwC_CG
Cuts at: 0 7235 7563
Size: 7235 328
SacI G_AGCT'C
Cuts at: 0 1177 2039 4661 5582 6875 7563
Size: 1177 862 2622 921 1293 688
Fragments arranged by size:
2622 1293 1177 921 862 688
SacII CC_GC'GG
Cuts at: 0 752 7563
Size: 752 6811
SalI G'TCGA_C
Cuts at: 0 5908 7563
Size: 5908 1655
SanDI GG'GwC_CC
Cuts at: 0 274 874 2198 5558 7563
Size: 274 600 1324 3360 2005
Fragments arranged by size:
3360 2005 1324 600 274
SapI GCTCTTCn'nnn_
Cuts at: 0 112 211 682 2044 3598 3763 6580 7563
Size: 112 99 471 1362 1554 165 2817 983
Fragments arranged by size:
2817 1554 1362 983 471 165 112 99
SbfI CC_TGCA'GG
Cuts at: 0 4667 7563
Size: 4667 2896
ScaI AGT'ACT
Cuts at: 0 1735 2953 7563
Size: 1735 1218 4610
Fragments arranged by size:
4610 1735 1218
SexAI A'CCwGG_T
Cuts at: 0 694 5357 5993 7563
Size: 694 4663 636 1570
Fragments arranged by size:
4663 1570 694 636
SfcI C'TryA_G
Cuts at: 0 282 508 1854 2086 3450 3610 3889 4585
Size: 282 226 1346 232 1364 160 279 696
Cuts at: 4585 4663 5779 7120 7222 7563
Size: 78 1116 1341 102 341
Fragments arranged by size:
1364 1346 1341 1116 696 341 282 279
232 226 160 102 78
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1197 7563
Size: 1197 6366
SfoI GGC'GCC
Cuts at: 0 421 768 2842 4957 7563
Size: 421 347 2074 2115 2606
Fragments arranged by size:
2606 2115 2074 421 347
SmaI CCC'GGG
Cuts at: 0 1218 4884 7563
Size: 1218 3666 2679
Fragments arranged by size:
3666 2679 1218
SmlI C'TyrA_G
Cuts at: 0 859 1876 2620 2947 3997 6640 7024 7432
Size: 859 1017 744 327 1050 2643 384 408
Cuts at: 7432 7563
Size: 131
Fragments arranged by size:
2643 1050 1017 859 744 408 384 327
131
StuI AGG'CCT
Cuts at: 0 1882 6715 7563
Size: 1882 4833 848
Fragments arranged by size:
4833 1882 848
StyI C'CwwG_G
Cuts at: 0 156 806 1454 1545 2555 3797 4139 5441
Size: 156 650 648 91 1010 1242 342 1302
Cuts at: 5441 6041 7256 7563
Size: 600 1215 307
Fragments arranged by size:
1302 1242 1215 1010 650 648 600 342
307 156 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2460 4341 6360 6514 7252 7563
Size: 2460 1881 2019 154 738 311
Fragments arranged by size:
2460 2019 1881 738 311 154
TatI w'GTAC_w
Cuts at: 0 29 1733 2459 2951 3659 4703 6290 6776
Size: 29 1704 726 492 708 1044 1587 486
Cuts at: 6776 7073 7563
Size: 297 490
Fragments arranged by size:
1704 1587 1044 726 708 492 490 486
297 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3439 3471 4643 4675 6358 6390 6932 6964
Size: 3439 32 1172 32 1683 32 542 32
Cuts at: 6964 7399 7431 7563
Size: 435 32 132
Fragments arranged by size:
3439 1683 1172 542 435 132 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3865 6793 7276 7563
Size: 3865 2928 483 287
Fragments arranged by size:
3865 2928 483 287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 2343 3804 4199 4455 4852 7563
Size: 360 1983 1461 395 256 397 2711
Fragments arranged by size:
2711 1983 1461 397 395 360 256
XhoI C'TCGA_G
Cuts at: 0 7024 7563
Size: 7024 539
XmaI C'CCGG_G
Cuts at: 0 1216 4882 7563
Size: 1216 3666 2681
Fragments arranged by size:
3666 2681 1216
XmnI GAAnn'nnTTC
Cuts at: 0 2764 7563
Size: 2764 4799
ZraI GAC'GTC
Cuts at: 0 2391 5877 7563
Size: 2391 3486 1686
Fragments arranged by size:
3486 2391 1686
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AloI AscI AseI AsiSI AvrII
BaeI BamHI BfrBI BsaBI BsiWI BsmI BspEI BspHI
BstBI BstZ17I CspCI DraI DrdI EcoRI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NsiI PacI
PmeI PsiI PvuI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI