Restriction map for
pF1KA0234
(Linear) (Six-base) MAPSORT of: KIAA0234.seq Check: 4376 from: 1 to: 4446
KIAA0234.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2550 2843 3354 4446
Size: 2550 293 511 1092
Fragments arranged by size:
2550 1092 511 293
AatII G_ACGT'C
Cuts at: 0 1836 4446
Size: 1836 2610
AccI GT'mk_AC
Cuts at: 0 459 1836 3767 4446
Size: 459 1377 1931 679
Fragments arranged by size:
1931 1377 679 459
AclI AA'CG_TT
Cuts at: 0 4187 4446
Size: 4187 259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1655 2107 2218 2263 2536 2558 3607
Size: 959 696 452 111 45 273 22 1049
Cuts at: 3607 3704 3758 4198 4367 4446
Size: 97 54 440 169 79
Fragments arranged by size:
1049 959 696 452 440 273 169 111
97 79 54 45 22
AfeI AGC'GCT
Cuts at: 0 2734 4446
Size: 2734 1712
AflIII A'CryG_T
Cuts at: 0 668 4446
Size: 668 3778
AleI CACnn'nnGTG
Cuts at: 0 36 749 4446
Size: 36 713 3697
Fragments arranged by size:
3697 713 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1309 3677 4446
Size: 1309 2368 769
Fragments arranged by size:
2368 1309 769
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2336 2368 4446
Size: 2336 32 2078
Fragments arranged by size:
2336 2078 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1792 2241 2520 2601 3112 3223 3591 3781
Size: 1792 449 279 81 511 111 368 190
Cuts at: 3781 4037 4047 4446
Size: 256 10 399
Fragments arranged by size:
1792 511 449 399 368 279 256 190
111 81 10
ApaLI G'TGCA_C
Cuts at: 0 1795 4446
Size: 1795 2651
ApoI r'AATT_y
Cuts at: 0 67 271 281 1069 1141 3066 3511 4079
Size: 67 204 10 788 72 1925 445 568
Cuts at: 4079 4105 4446
Size: 26 341
Fragments arranged by size:
1925 788 568 445 341 204 72 67
26 10
AvaI C'yCGr_G
Cuts at: 0 2696 4446
Size: 2696 1750
BanI G'GyrC_C
Cuts at: 0 899 1044 1293 2532 2659 3315 3366 4446
Size: 899 145 249 1239 127 656 51 1080
Fragments arranged by size:
1239 1080 899 656 249 145 127 51
BanII G_rGCy'C
Cuts at: 0 710 1739 2066 2626 3895 3972 3986 4143
Size: 710 1029 327 560 1269 77 14 157
Cuts at: 4143 4230 4237 4446
Size: 87 7 209
Fragments arranged by size:
1269 1029 710 560 327 209 157 87
77 14 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 1399 1713 4446
Size: 1106 293 314 2733
Fragments arranged by size:
2733 1106 314 293
BbvCI CC'TCA_GC
Cuts at: 0 2444 4446
Size: 2444 2002
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3265 3299 4446
Size: 3265 34 1147
Fragments arranged by size:
3265 1147 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3280 4446
Size: 3280 1166
BfrBI ATG'CAT
Cuts at: 0 2391 4446
Size: 2391 2055
BglI GCCn_nnn'nGGC
Cuts at: 0 1976 2979 3600 3670 4446
Size: 1976 1003 621 70 776
Fragments arranged by size:
1976 1003 776 621 70
BglII A'GATC_T
Cuts at: 0 323 4446
Size: 323 4123
BlpI GC'TnA_GC
Cuts at: 0 1874 2702 3716 4446
Size: 1874 828 1014 730
Fragments arranged by size:
1874 1014 828 730
Bme1580I G_kGCm'C
Cuts at: 0 43 756 904 1298 1799 2187 3440 4446
Size: 43 713 148 394 501 388 1253 1006
Fragments arranged by size:
1253 1006 713 501 394 388 148 43
BmgBI CAC'GTC
Cuts at: 0 1104 4446
Size: 1104 3342
BmrI ACTGGGnnnn_n'
Cuts at: 0 750 3299 3458 4446
Size: 750 2549 159 988
Fragments arranged by size:
2549 988 750 159
BmtI G_CTAG'C
Cuts at: 0 3490 4446
Size: 3490 956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3060 3777 4157 4446
Size: 3060 717 380 289
Fragments arranged by size:
3060 717 380 289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 915 1095 1374 2209 2658 2782 3051
Size: 244 671 180 279 835 449 124 269
Cuts at: 3051 3862 3866 3922 3997 4049 4114 4165 4446
Size: 811 4 56 75 52 65 51 281
Fragments arranged by size:
835 811 671 449 281 279 269 244
180 124 75 65 56 52 51 4
Bpu10I CC'TnA_GC
Cuts at: 0 584 1460 2179 2444 4236 4446
Size: 584 876 719 265 1792 210
Fragments arranged by size:
1792 876 719 584 265 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 717 2264 2380 3043 3106 4027 4446
Size: 670 47 1547 116 663 63 921 419
Fragments arranged by size:
1547 921 670 663 419 116 63 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 3206 4446
Size: 3206 1240
BsaAI yAC'GTr
Cuts at: 0 1308 1937 4446
Size: 1308 629 2509
Fragments arranged by size:
2509 1308 629
BsaBI GATnn'nnATC
Cuts at: 0 1778 4446
Size: 1778 2668
BsaHI Gr'CG_yC
Cuts at: 0 1677 1833 1862 4446
Size: 1677 156 29 2584
Fragments arranged by size:
2584 1677 156 29
BsaWI w'CCGG_w
Cuts at: 0 33 2039 4446
Size: 33 2006 2407
Fragments arranged by size:
2407 2006 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1186 1216 2021 2051 3462 3492 3842 3872
Size: 1186 30 805 30 1411 30 350 30
Cuts at: 3872 3895 3925 4201 4231 4446
Size: 23 30 276 30 215
Fragments arranged by size:
1411 1186 805 350 276 215 30 30
30 30 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 188 282 514 1207 1450 1747 1840 2223
Size: 188 94 232 693 243 297 93 383
Cuts at: 2223 3436 3769 3994 4446
Size: 1213 333 225 452
Fragments arranged by size:
1213 693 452 383 333 297 243 232
225 188 94 93
BseYI C'CCAG_C
Cuts at: 0 59 2520 2735 3156 3186 3373 4446
Size: 59 2461 215 421 30 187 1073
Fragments arranged by size:
2461 1073 421 215 187 59 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 696 1586 3125 3182 4446
Size: 451 245 890 1539 57 1264
Fragments arranged by size:
1539 1264 890 451 245 57
BsiHKAI G_wGCw'C
Cuts at: 0 1739 1799 2020 2066 3986 4237 4446
Size: 1739 60 221 46 1920 251 209
Fragments arranged by size:
1920 1739 251 221 209 60 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1759 4446
Size: 1759 2687
Bsp1286I G_dGCh'C
Cuts at: 0 43 710 756 904 1298 1739 1799 2020
Size: 43 667 46 148 394 441 60 221
Cuts at: 2020 2066 2187 2626 3440 3895 3972 3986 4143
Size: 46 121 439 814 455 77 14 157
Cuts at: 4143 4230 4237 4446
Size: 87 7 209
Fragments arranged by size:
814 667 455 441 439 394 221 209
157 148 121 87 77 60 46 46
43 14 7
BspHI T'CATG_A
Cuts at: 0 1508 3986 4446
Size: 1508 2478 460
Fragments arranged by size:
2478 1508 460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1677 2550 2843 2978 3000 3354 4010 4446
Size: 1677 873 293 135 22 354 656 436
Fragments arranged by size:
1677 873 656 436 354 293 135 22
BsrBI CCG'CTC
Cuts at: 0 318 4446
Size: 318 4128
BsrDI GCAATG_nn'
Cuts at: 0 89 358 679 2581 2723 4446
Size: 89 269 321 1902 142 1723
Fragments arranged by size:
1902 1723 321 269 142 89
BsrFI r'CCGG_y
Cuts at: 0 1700 3137 4446
Size: 1700 1437 1309
Fragments arranged by size:
1700 1437 1309
BsrGI T'GTAC_A
Cuts at: 0 1035 4446
Size: 1035 3411
BssSI C'ACGA_G
Cuts at: 0 1592 1729 2201 2450 4446
Size: 1592 137 472 249 1996
Fragments arranged by size:
1996 1592 472 249 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1925 3591 4047 4446
Size: 1925 1666 456 399
Fragments arranged by size:
1925 1666 456 399
BstBI TT'CG_AA
Cuts at: 0 735 4446
Size: 735 3711
BstEII G'GTnAC_C
Cuts at: 0 2914 4011 4446
Size: 2914 1097 435
Fragments arranged by size:
2914 1097 435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 564 3312 4446
Size: 564 2748 1134
Fragments arranged by size:
2748 1134 564
BstYI r'GATC_y
Cuts at: 0 323 1773 2903 4446
Size: 323 1450 1130 1543
Fragments arranged by size:
1543 1450 1130 323
Bsu36I CC'TnA_GG
Cuts at: 0 1190 3608 3956 4446
Size: 1190 2418 348 490
Fragments arranged by size:
2418 1190 490 348
BtgI C'CryG_G
Cuts at: 0 146 792 1533 3417 4446
Size: 146 646 741 1884 1029
Fragments arranged by size:
1884 1029 741 646 146
BtsI GCAGTG_nn'
Cuts at: 0 756 1155 2467 3079 3397 3430 3481 3779
Size: 756 399 1312 612 318 33 51 298
Cuts at: 3779 3813 4446
Size: 34 633
Fragments arranged by size:
1312 756 633 612 399 318 298 51
34 33
DraI TTT'AAA
Cuts at: 0 299 4446
Size: 299 4147
EaeI y'GGCC_r
Cuts at: 0 2154 2309 2462 3544 4446
Size: 2154 155 153 1082 902
Fragments arranged by size:
2154 1082 902 155 153
EarI CTCTTCn'nnn_
Cuts at: 0 2168 2981 3339 4386 4446
Size: 2168 813 358 1047 60
Fragments arranged by size:
2168 1047 813 358 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 934 4446
Size: 127 807 3512
Fragments arranged by size:
3512 807 127
EcoICRI GAG'CTC
Cuts at: 0 1737 2064 3984 4235 4446
Size: 1737 327 1920 251 211
Fragments arranged by size:
1920 1737 327 251 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 915 959 1095 1374 1655 2107 2209
Size: 244 671 44 136 279 281 452 102
Cuts at: 2209 2218 2263 2536 2558 2658 2782 3051 3607
Size: 9 45 273 22 100 124 269 556
Cuts at: 3607 3704 3758 3862 3866 3922 3997 4049 4114
Size: 97 54 104 4 56 75 52 65
Cuts at: 4114 4165 4198 4367 4446
Size: 51 33 169 79
Fragments arranged by size:
671 556 452 281 279 273 269 244
169 136 124 104 102 100 97 79
75 65 56 54 52 51 45 44
33 22 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2257 2422 3403 4006 4446
Size: 2257 165 981 603 440
Fragments arranged by size:
2257 981 603 440 165
EcoO109I rG'GnC_Cy
Cuts at: 0 553 1845 2352 2598 2946 2967 3204 3618
Size: 553 1292 507 246 348 21 237 414
Cuts at: 3618 3999 4446
Size: 381 447
Fragments arranged by size:
1292 553 507 447 414 381 348 246
237 21
EcoRI G'AATT_C
Cuts at: 0 67 1069 3066 4079 4446
Size: 67 1002 1997 1013 367
Fragments arranged by size:
1997 1013 1002 367 67
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 97 711 4446
Size: 97 614 3735
Fragments arranged by size:
3735 614 97
HaeII r_GCGC'y
Cuts at: 0 1713 2736 3157 4446
Size: 1713 1023 421 1289
Fragments arranged by size:
1713 1289 1023 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1758 1790 2021 2053 2307 2339 3100 3132
Size: 1758 32 231 32 254 32 761 32
Cuts at: 3132 3205 3237 3895 3927 4201 4233 4265 4363
Size: 73 32 658 32 274 32 32 98
Cuts at: 4363 4395 4446
Size: 32 51
Fragments arranged by size:
1758 761 658 274 254 231 98 73
51 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 0 180 460 1644 3932 4446
Size: 180 280 1184 2288 514
Fragments arranged by size:
2288 1184 514 280 180
HindIII A'AGCT_T
Cuts at: 0 811 941 2879 4446
Size: 811 130 1938 1567
Fragments arranged by size:
1938 1567 811 130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1198 1751 2483 3364 3926 4105 4446
Size: 1040 158 553 732 881 562 179 341
Fragments arranged by size:
1040 881 732 562 553 341 179 158
MscI TGG'CCA
Cuts at: 0 2311 2464 4446
Size: 2311 153 1982
Fragments arranged by size:
2311 1982 153
MslI CAynn'nnrTG
Cuts at: 0 36 749 1268 1291 1382 1507 1525 2000
Size: 36 713 519 23 91 125 18 475
Cuts at: 2000 4446
Size: 2446
Fragments arranged by size:
2446 713 519 475 125 91 36 23
18
MspA1I CmG'CkG
Cuts at: 0 148 2331 2520 3279 3373 3711 4044 4387
Size: 148 2183 189 759 94 338 333 343
Cuts at: 4387 4446
Size: 59
Fragments arranged by size:
2183 759 343 338 333 189 148 94
59
NaeI GCC'GGC
Cuts at: 0 1702 4446
Size: 1702 2744
NcoI C'CATG_G
Cuts at: 0 1533 3417 4446
Size: 1533 1884 1029
Fragments arranged by size:
1884 1533 1029
NdeI CA'TA_TG
Cuts at: 0 1040 4446
Size: 1040 3406
NgoMIV G'CCGG_C
Cuts at: 0 1700 4446
Size: 1700 2746
NheI G'CTAG_C
Cuts at: 0 3486 4446
Size: 3486 960
NsiI A_TGCA'T
Cuts at: 0 2393 4446
Size: 2393 2053
NspI r_CATG'y
Cuts at: 0 623 1032 4446
Size: 623 409 3414
Fragments arranged by size:
3414 623 409
PflMI CCAn_nnn'nTGG
Cuts at: 0 1217 3650 4446
Size: 1217 2433 796
Fragments arranged by size:
2433 1217 796
PfoI T'CCnGG_A
Cuts at: 0 2417 3028 3882 4446
Size: 2417 611 854 564
Fragments arranged by size:
2417 854 611 564
PmlI CAC'GTG
Cuts at: 0 1937 4446
Size: 1937 2509
PpuMI rG'GwC_Cy
Cuts at: 0 553 2352 2967 3204 3999 4446
Size: 553 1799 615 237 795 447
Fragments arranged by size:
1799 795 615 553 447 237
PshAI GACnn'nnGTC
Cuts at: 0 3401 4446
Size: 3401 1045
PstI C_TGCA'G
Cuts at: 0 3011 3278 3392 3587 4052 4446
Size: 3011 267 114 195 465 394
Fragments arranged by size:
3011 465 394 267 195 114
PvuII CAG'CTG
Cuts at: 0 2331 2520 3279 3373 3711 4044 4387 4446
Size: 2331 189 759 94 338 333 343 59
Fragments arranged by size:
2331 759 343 338 333 189 94 59
SacI G_AGCT'C
Cuts at: 0 1739 2066 3986 4237 4446
Size: 1739 327 1920 251 209
Fragments arranged by size:
1920 1739 327 251 209
SacII CC_GC'GG
Cuts at: 0 149 4446
Size: 149 4297
SalI G'TCGA_C
Cuts at: 0 458 4446
Size: 458 3988
SapI GCTCTTCn'nnn_
Cuts at: 0 2168 2981 4386 4446
Size: 2168 813 1405 60
Fragments arranged by size:
2168 1405 813 60
SexAI A'CCwGG_T
Cuts at: 0 1396 4446
Size: 1396 3050
SfcI C'TryA_G
Cuts at: 0 336 453 475 494 1049 3007 3274 3388
Size: 336 117 22 19 555 1958 267 114
Cuts at: 3388 3583 3733 4048 4446
Size: 195 150 315 398
Fragments arranged by size:
1958 555 398 336 315 267 195 150
117 114 22 19
SmaI CCC'GGG
Cuts at: 0 2698 4446
Size: 2698 1748
SmlI C'TyrA_G
Cuts at: 0 685 696 2279 2359 3058 3085 4006 4446
Size: 685 11 1583 80 699 27 921 440
Fragments arranged by size:
1583 921 699 685 440 80 27 11
SrfI GCCC'GGGC
Cuts at: 0 2698 4446
Size: 2698 1748
SspI AAT'ATT
Cuts at: 0 3819 4446
Size: 3819 627
StuI AGG'CCT
Cuts at: 0 2434 3043 3760 4446
Size: 2434 609 717 686
Fragments arranged by size:
2434 717 686 609
StyI C'CwwG_G
Cuts at: 0 1533 1617 2054 2138 3417 3831 4446
Size: 1533 84 437 84 1279 414 615
Fragments arranged by size:
1533 1279 615 437 414 84 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2512 2647 4446
Size: 2512 135 1799
Fragments arranged by size:
2512 1799 135
TatI w'GTAC_w
Cuts at: 0 971 1035 1651 4446
Size: 971 64 616 2795
Fragments arranged by size:
2795 971 616 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 3462 3494 3842 3874 4446
Size: 27 3435 32 348 32 572
Fragments arranged by size:
3435 572 348 32 32 27
XbaI T'CTAG_A
Cuts at: 0 2931 4446
Size: 2931 1515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 79 149 2682 3488 3672 4446
Size: 79 70 2533 806 184 774
Fragments arranged by size:
2533 806 774 184 79 70
XmaI C'CCGG_G
Cuts at: 0 2696 4446
Size: 2696 1750
XmnI GAAnn'nnTTC
Cuts at: 0 1069 4446
Size: 1069 3377
ZraI GAC'GTC
Cuts at: 0 1834 4446
Size: 1834 2612
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AvaI BanI BanII
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NsiI NspI PflMI PfoI PmlI PpuMI
PshAI PstI PvuII SacI SacII SalI SapI SexAI
SfcI SmaI SmlI SrfI SspI StuI StyI TaqII
TatI TstI XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AgeI AhdI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BclI BsiEI BsiWI BsmI
BspEI BssHII BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI NarI NotI NruI PacI PasI PciI
PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SwaI Tth111I XhoI