(Linear) (Six-base) MAPSORT of: KIAA0236.seq Check: 1037 from: 1 to: 5061 KIAA0236.orf With 182 enzymes: SgfI * March 3, 2008 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 551 2370 2660 2790 5000 5061 Size: 551 1819 290 130 2210 61 Fragments arranged by size: 2210 1819 551 290 130 61 Acc65I G'GTAC_C Cuts at: 0 2740 3635 3712 5061 Size: 2740 895 77 1349 Fragments arranged by size: 2740 1349 895 77 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 374 940 1731 2276 2677 3067 3158 Size: 218 156 566 791 545 401 390 91 Cuts at: 3158 4202 4618 5061 Size: 1044 416 443 Fragments arranged by size: 1044 791 566 545 443 416 401 390 218 156 91 AfeI AGC'GCT Cuts at: 0 508 5061 Size: 508 4553 AflII C'TTAA_G Cuts at: 0 3447 3901 5061 Size: 3447 454 1160 Fragments arranged by size: 3447 1160 454 AflIII A'CryG_T Cuts at: 0 1370 3322 5061 Size: 1370 1952 1739 Fragments arranged by size: 1952 1739 1370 AgeI A'CCGG_T Cuts at: 0 3638 3737 5061 Size: 3638 99 1324 Fragments arranged by size: 3638 1324 99 AleI CACnn'nnGTG Cuts at: 0 677 1289 5061 Size: 677 612 3772 Fragments arranged by size: 3772 677 612 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2668 3494 5061 Size: 2668 826 1567 Fragments arranged by size: 2668 1567 826 AlwNI CAG_nnn'CTG Cuts at: 0 196 241 441 1965 2287 2878 3416 5061 Size: 196 45 200 1524 322 591 538 1645 Fragments arranged by size: 1645 1524 591 538 322 200 196 45 ApaI G_GGCC'C Cuts at: 0 1861 3500 3979 5061 Size: 1861 1639 479 1082 Fragments arranged by size: 1861 1639 1082 479 ApaLI G'TGCA_C Cuts at: 0 1374 1420 4359 4657 4987 5061 Size: 1374 46 2939 298 330 74 Fragments arranged by size: 2939 1374 330 298 74 46 ApoI r'AATT_y Cuts at: 0 781 5061 Size: 781 4280 AvaI C'yCGr_G Cuts at: 0 137 2605 3385 3928 4166 4615 5061 Size: 137 2468 780 543 238 449 446 Fragments arranged by size: 2468 780 543 449 446 238 137 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 4504 4537 5061 Size: 4504 33 524 Fragments arranged by size: 4504 524 33 BamHI G'GATC_C Cuts at: 0 4499 5013 5061 Size: 4499 514 48 Fragments arranged by size: 4499 514 48 BanI G'GyrC_C Cuts at: 0 463 2573 2740 3470 3635 3712 3844 4925 Size: 463 2110 167 730 165 77 132 1081 Cuts at: 4925 5061 Size: 136 Fragments arranged by size: 2110 1081 730 463 167 165 136 132 77 BanII G_rGCy'C Cuts at: 0 127 435 1861 1886 2294 2594 3306 3354 Size: 127 308 1426 25 408 300 712 48 Cuts at: 3354 3500 3979 5061 Size: 146 479 1082 Fragments arranged by size: 1426 1082 712 479 408 308 300 146 127 48 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 2004 5061 Size: 2004 3057 BbvCI CC'TCA_GC Cuts at: 0 790 2641 5061 Size: 790 1851 2420 Fragments arranged by size: 2420 1851 790 BciVI GTATCCnnnnn_n' Cuts at: 0 3267 5061 Size: 3267 1794 BclI T'GATC_A Cuts at: 0 1491 5061 Size: 1491 3570 BfrBI ATG'CAT Cuts at: 0 723 1182 5061 Size: 723 459 3879 Fragments arranged by size: 3879 723 459 BglI GCCn_nnn'nGGC Cuts at: 0 1000 1100 3842 5061 Size: 1000 100 2742 1219 Fragments arranged by size: 2742 1219 1000 100 BlpI GC'TnA_GC Cuts at: 0 434 1498 1678 5061 Size: 434 1064 180 3383 Fragments arranged by size: 3383 1064 434 180 Bme1580I G_kGCm'C Cuts at: 0 64 580 1378 1424 1728 1861 2605 2668 Size: 64 516 798 46 304 133 744 63 Cuts at: 2668 3500 3607 3979 4012 4345 4363 4531 4661 Size: 832 107 372 33 333 18 168 130 Cuts at: 4661 4991 5061 Size: 330 70 Fragments arranged by size: 832 798 744 516 372 333 330 304 168 133 130 107 70 64 63 46 33 18 BmgBI CAC'GTC Cuts at: 0 1320 3157 5061 Size: 1320 1837 1904 Fragments arranged by size: 1904 1837 1320 BmrI ACTGGGnnnn_n' Cuts at: 0 1144 2343 2579 2881 2941 3001 3861 3873 Size: 1144 1199 236 302 60 60 860 12 Cuts at: 3873 4433 5061 Size: 560 628 Fragments arranged by size: 1199 1144 860 628 560 302 236 60 60 12 BmtI G_CTAG'C Cuts at: 0 2621 5061 Size: 2621 2440 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3276 5061 Size: 3276 1785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 278 287 556 1820 2131 2547 3416 5029 Size: 278 9 269 1264 311 416 869 1613 Cuts at: 5029 5061 Size: 32 Fragments arranged by size: 1613 1264 869 416 311 278 269 32 9 Bpu10I CC'TnA_GC Cuts at: 0 65 790 2641 5061 Size: 65 725 1851 2420 Fragments arranged by size: 2420 1851 725 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 601 799 2155 2395 3559 4081 4468 5061 Size: 601 198 1356 240 1164 522 387 593 Fragments arranged by size: 1356 1164 601 593 522 387 240 198 BsaI GGTCTCn'nnnn_ Cuts at: 0 265 462 919 1796 3958 4660 5061 Size: 265 197 457 877 2162 702 401 Fragments arranged by size: 2162 877 702 457 401 265 197 BsaAI yAC'GTr Cuts at: 0 3132 4917 5061 Size: 3132 1785 144 Fragments arranged by size: 3132 1785 144 BsaWI w'CCGG_w Cuts at: 0 3638 3737 4115 5061 Size: 3638 99 378 946 Fragments arranged by size: 3638 946 378 99 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 264 294 1453 1483 2289 2319 2717 2747 Size: 264 30 1159 30 806 30 398 30 Cuts at: 2747 3070 3100 5061 Size: 323 30 1961 Fragments arranged by size: 1961 1159 806 398 323 264 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 373 1996 2080 2152 2332 2958 3018 3105 Size: 373 1623 84 72 180 626 60 87 Cuts at: 3105 3403 4225 5061 Size: 298 822 836 Fragments arranged by size: 1623 836 822 626 373 298 180 87 84 72 60 BseYI C'CCAG_C Cuts at: 0 276 565 639 1598 1948 2047 2052 3191 Size: 276 289 74 959 350 99 5 1139 Cuts at: 3191 3486 3849 4012 4808 5017 5061 Size: 295 363 163 796 209 44 Fragments arranged by size: 1139 959 796 363 350 295 289 276 209 163 99 74 44 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 1104 2061 2491 2542 3388 3920 3967 Size: 131 973 957 430 51 846 532 47 Cuts at: 3967 4614 5023 5061 Size: 647 409 38 Fragments arranged by size: 973 957 846 647 532 430 409 131 51 47 38 BsiEI CG_ry'CG Cuts at: 0 897 4851 5061 Size: 897 3954 210 Fragments arranged by size: 3954 897 210 BsiHKAI G_wGCw'C Cuts at: 0 264 542 812 1247 1378 1424 1471 2735 Size: 264 278 270 435 131 46 47 1264 Cuts at: 2735 4115 4363 4661 4991 5061 Size: 1380 248 298 330 70 Fragments arranged by size: 1380 1264 435 330 298 278 270 264 248 131 70 47 46 BsiWI C'GTAC_G Cuts at: 0 4917 5061 Size: 4917 144 BsmI GAATG_Cn' Cuts at: 0 699 5061 Size: 699 4362 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1310 4354 4486 5061 Size: 1310 3044 132 575 Fragments arranged by size: 3044 1310 575 132 Bsp1286I G_dGCh'C Cuts at: 0 64 127 264 435 542 580 812 1247 Size: 64 63 137 171 107 38 232 435 Cuts at: 1247 1378 1424 1471 1728 1861 1886 2294 2594 Size: 131 46 47 257 133 25 408 300 Cuts at: 2594 2605 2668 2735 3306 3354 3500 3607 3979 Size: 11 63 67 571 48 146 107 372 Cuts at: 3979 4012 4115 4345 4363 4531 4661 4991 5061 Size: 33 103 230 18 168 130 330 70 Fragments arranged by size: 571 435 408 372 330 300 257 232 230 171 168 146 137 133 131 130 107 107 103 70 67 64 63 63 48 47 46 38 33 25 18 11 BspHI T'CATG_A Cuts at: 0 1188 5061 Size: 1188 3873 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 313 551 2370 2660 2790 5000 5061 Size: 313 238 1819 290 130 2210 61 Fragments arranged by size: 2210 1819 313 290 238 130 61 BsrBI CCG'CTC Cuts at: 0 4173 5038 5061 Size: 4173 865 23 Fragments arranged by size: 4173 865 23 BsrDI GCAATG_nn' Cuts at: 0 3222 4791 5061 Size: 3222 1569 270 Fragments arranged by size: 3222 1569 270 BsrFI r'CCGG_y Cuts at: 0 3566 3638 3737 5061 Size: 3566 72 99 1324 Fragments arranged by size: 3566 1324 99 72 BssSI C'ACGA_G Cuts at: 0 3580 5061 Size: 3580 1481 BstAPI GCAn_nnn'nTGC Cuts at: 0 1127 1466 1674 2730 3089 3416 3686 3695 Size: 1127 339 208 1056 359 327 270 9 Cuts at: 3695 3915 3943 4790 5001 5061 Size: 220 28 847 211 60 Fragments arranged by size: 1127 1056 847 359 339 327 270 220 211 208 60 28 9 BstEII G'GTnAC_C Cuts at: 0 2029 5061 Size: 2029 3032 BstXI CCAn_nnnn'nTGG Cuts at: 0 3709 5061 Size: 3709 1352 BstYI r'GATC_y Cuts at: 0 1196 3368 4499 5013 5061 Size: 1196 2172 1131 514 48 Fragments arranged by size: 2172 1196 1131 514 48 Bsu36I CC'TnA_GG Cuts at: 0 1447 1910 2419 5054 5061 Size: 1447 463 509 2635 7 Fragments arranged by size: 2635 1447 509 463 7 BtgI C'CryG_G Cuts at: 0 99 930 995 1785 2022 2975 3492 4054 Size: 99 831 65 790 237 953 517 562 Cuts at: 4054 4226 5061 Size: 172 835 Fragments arranged by size: 953 835 831 790 562 517 237 172 99 65 BtsI GCAGTG_nn' Cuts at: 0 657 1029 1110 1226 2016 2517 2934 3078 Size: 657 372 81 116 790 501 417 144 Cuts at: 3078 3405 3425 3440 3476 4272 4648 4711 5061 Size: 327 20 15 36 796 376 63 350 Fragments arranged by size: 796 790 657 501 417 376 372 350 327 144 116 81 63 36 20 15 DraIII CAC_nnn'GTG Cuts at: 0 1400 3784 4326 4654 5061 Size: 1400 2384 542 328 407 Fragments arranged by size: 2384 1400 542 407 328 EaeI y'GGCC_r Cuts at: 0 713 3675 4257 5061 Size: 713 2962 582 804 Fragments arranged by size: 2962 804 713 582 EarI CTCTTCn'nnn_ Cuts at: 0 194 350 371 376 481 1103 2852 5061 Size: 194 156 21 5 105 622 1749 2209 Fragments arranged by size: 2209 1749 622 194 156 105 21 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 775 5061 Size: 775 4286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 278 287 374 556 940 1731 1820 Size: 218 60 9 87 182 384 791 89 Cuts at: 1820 2131 2276 2547 2677 3067 3158 3416 4202 Size: 311 145 271 130 390 91 258 786 Cuts at: 4202 4618 5029 5061 Size: 416 411 32 Fragments arranged by size: 791 786 416 411 390 384 311 271 258 218 182 145 130 91 89 87 60 32 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 793 2137 2897 2957 3017 5061 Size: 793 1344 760 60 60 2044 Fragments arranged by size: 2044 1344 793 760 60 60 EcoO109I rG'GnC_Cy Cuts at: 0 112 141 798 1443 1857 1962 2094 2171 Size: 112 29 657 645 414 105 132 77 Cuts at: 2171 2415 2547 2922 2982 3042 3179 3497 3975 Size: 244 132 375 60 60 137 318 478 Cuts at: 3975 3976 4070 5061 Size: 1 94 991 Fragments arranged by size: 991 657 645 478 414 375 318 244 137 132 132 112 105 94 77 60 60 29 1 EcoRI G'AATT_C Cuts at: 0 781 5061 Size: 781 4280 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 528 2881 2955 3075 4746 5061 Size: 528 2353 74 120 1671 315 Fragments arranged by size: 2353 1671 528 315 120 74 FspI TGC'GCA Cuts at: 0 4741 5061 Size: 4741 320 HaeII r_GCGC'y Cuts at: 0 510 5061 Size: 510 4551 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 114 146 264 296 442 474 1170 1202 Size: 114 32 118 32 146 32 696 32 Cuts at: 1202 2098 2130 2386 2418 4382 4414 4659 4691 Size: 896 32 256 32 1964 32 245 32 Cuts at: 4691 5061 Size: 370 Fragments arranged by size: 1964 896 696 370 256 245 146 118 114 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1989 3828 4560 5061 Size: 1989 1839 732 501 Fragments arranged by size: 1989 1839 732 501 KpnI G_GTAC'C Cuts at: 0 2744 3639 3716 5061 Size: 2744 895 77 1345 Fragments arranged by size: 2744 1345 895 77 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 761 1275 1379 5061 Size: 761 514 104 3682 Fragments arranged by size: 3682 761 514 104 MscI TGG'CCA Cuts at: 0 715 4259 5061 Size: 715 3544 802 Fragments arranged by size: 3544 802 715 MslI CAynn'nnrTG Cuts at: 0 677 1187 1271 1289 1790 2027 4608 4994 Size: 677 510 84 18 501 237 2581 386 Cuts at: 4994 5061 Size: 67 Fragments arranged by size: 2581 677 510 501 386 237 84 67 18 MspA1I CmG'CkG Cuts at: 0 352 438 1497 1896 2075 2510 3833 3995 Size: 352 86 1059 399 179 435 1323 162 Cuts at: 3995 4142 4308 4566 4808 5007 5061 Size: 147 166 258 242 199 54 Fragments arranged by size: 1323 1059 435 399 352 258 242 199 179 166 162 147 86 54 NcoI C'CATG_G Cuts at: 0 930 995 1785 2022 5061 Size: 930 65 790 237 3039 Fragments arranged by size: 3039 930 790 237 65 NdeI CA'TA_TG Cuts at: 0 488 5061 Size: 488 4573 NheI G'CTAG_C Cuts at: 0 2617 5061 Size: 2617 2444 NsiI A_TGCA'T Cuts at: 0 725 1184 5061 Size: 725 459 3877 Fragments arranged by size: 3877 725 459 NspI r_CATG'y Cuts at: 0 723 1186 1260 1313 1344 1374 1428 1438 Size: 723 463 74 53 31 30 54 10 Cuts at: 1438 3326 3558 3913 4298 4497 5061 Size: 1888 232 355 385 199 564 Fragments arranged by size: 1888 723 564 463 385 355 232 199 74 54 53 31 30 10 PasI CC'CwG_GG Cuts at: 0 2097 2175 2837 5061 Size: 2097 78 662 2224 Fragments arranged by size: 2224 2097 662 78 PciI A'CATG_T Cuts at: 0 1370 3322 5061 Size: 1370 1952 1739 Fragments arranged by size: 1952 1739 1370 PflMI CCAn_nnn'nTGG Cuts at: 0 3493 4308 4959 5061 Size: 3493 815 651 102 Fragments arranged by size: 3493 815 651 102 PfoI T'CCnGG_A Cuts at: 0 264 294 2563 5061 Size: 264 30 2269 2498 Fragments arranged by size: 2498 2269 264 30 PmlI CAC'GTG Cuts at: 0 3132 5061 Size: 3132 1929 PpuMI rG'GwC_Cy Cuts at: 0 141 1443 3179 5061 Size: 141 1302 1736 1882 Fragments arranged by size: 1882 1736 1302 141 PspOMI G'GGCC_C Cuts at: 0 1857 3496 3975 5061 Size: 1857 1639 479 1086 Fragments arranged by size: 1857 1639 1086 479 PspXI vC'TCGA_Gb Cuts at: 0 3385 5061 Size: 3385 1676 PstI C_TGCA'G Cuts at: 0 880 1583 1648 2901 2961 3021 3148 3412 Size: 880 703 65 1253 60 60 127 264 Cuts at: 3412 3483 3691 3963 5061 Size: 71 208 272 1098 Fragments arranged by size: 1253 1098 880 703 272 264 208 127 71 65 60 60 PvuII CAG'CTG Cuts at: 0 352 1497 1896 2075 3833 4308 4566 5007 Size: 352 1145 399 179 1758 475 258 441 Cuts at: 5007 5061 Size: 54 Fragments arranged by size: 1758 1145 475 441 399 352 258 179 54 SanDI GG'GwC_CC Cuts at: 0 141 3179 5061 Size: 141 3038 1882 Fragments arranged by size: 3038 1882 141 SapI GCTCTTCn'nnn_ Cuts at: 0 1103 2852 5061 Size: 1103 1749 2209 Fragments arranged by size: 2209 1749 1103 SbfI CC_TGCA'GG Cuts at: 0 2901 2961 3021 5061 Size: 2901 60 60 2040 Fragments arranged by size: 2901 2040 60 60 ScaI AGT'ACT Cuts at: 0 4627 5061 Size: 4627 434 SfcI C'TryA_G Cuts at: 0 675 876 1579 1644 2897 2957 3017 3144 Size: 675 201 703 65 1253 60 60 127 Cuts at: 3144 3408 3479 3511 3687 3789 3959 5061 Size: 264 71 32 176 102 170 1102 Fragments arranged by size: 1253 1102 703 675 264 201 176 170 127 102 71 65 60 60 32 SgrAI Cr'CCGG_yG Cuts at: 0 3566 5061 Size: 3566 1495 SmaI CCC'GGG Cuts at: 0 139 4168 5061 Size: 139 4029 893 Fragments arranged by size: 4029 893 139 SmlI C'TyrA_G Cuts at: 0 616 814 2134 2410 3385 3447 3574 3901 Size: 616 198 1320 276 975 62 127 327 Cuts at: 3901 4060 4483 5061 Size: 159 423 578 Fragments arranged by size: 1320 975 616 578 423 327 276 198 159 127 62 SphI G_CATG'C Cuts at: 0 723 1186 3558 3913 5061 Size: 723 463 2372 355 1148 Fragments arranged by size: 2372 1148 723 463 355 StuI AGG'CCT Cuts at: 0 904 1810 2749 4084 4717 5061 Size: 904 906 939 1335 633 344 Fragments arranged by size: 1335 939 906 904 633 344 StyI C'CwwG_G Cuts at: 0 426 930 995 1361 1785 2022 2577 3840 Size: 426 504 65 366 424 237 555 1263 Cuts at: 3840 5061 Size: 1221 Fragments arranged by size: 1263 1221 555 504 426 424 366 237 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 406 631 2026 2239 2561 2662 2727 2988 Size: 406 225 1395 213 322 101 65 261 Cuts at: 2988 3048 4067 4759 4855 5061 Size: 60 1019 692 96 206 Fragments arranged by size: 1395 1019 692 406 322 261 225 213 206 101 96 65 60 TatI w'GTAC_w Cuts at: 0 3247 4625 5061 Size: 3247 1378 436 Fragments arranged by size: 3247 1378 436 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 43 75 1357 1389 1579 1611 1751 1783 Size: 43 32 1282 32 190 32 140 32 Cuts at: 1783 2289 2321 3070 3102 5061 Size: 506 32 749 32 1959 Fragments arranged by size: 1959 1282 749 506 190 140 43 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2947 3007 5061 Size: 2947 60 2054 Fragments arranged by size: 2947 2054 60 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1606 3514 5061 Size: 1606 1908 1547 Fragments arranged by size: 1908 1606 1547 XhoI C'TCGA_G Cuts at: 0 3385 5061 Size: 3385 1676 XmaI C'CCGG_G Cuts at: 0 137 4166 5061 Size: 137 4029 895 Fragments arranged by size: 4029 895 137 XmnI GAAnn'nnTTC Cuts at: 0 595 5061 Size: 595 4466 Enzymes that do cut: AarI Acc65I AcuI AfeI AflII AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SanDI SapI SbfI ScaI SfcI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AhdI AloI AscI AseI AsiSI AvrII BbeI BcgI BglII BsaBI BsaHI BspEI BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRV FseI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NgoMIV NotI NruI PacI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SexAI SfiI SfoI SgfI SnaBI SpeI SrfI SspI SwaI XbaI ZraI