Restriction map for pF1KA0236
 (Linear) (Six-base) MAPSORT of: KIAA0236.seq  Check: 1037  from: 1  to: 5061



KIAA0236.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     551    2370    2660    2790    5000    5061 
   Size:        551    1819     290     130    2210      61

  Fragments arranged by size: 

                2210    1819     551     290     130      61
 
Acc65I G'GTAC_C

Cuts at:      0    2740    3635    3712    5061 
   Size:       2740     895      77    1349

  Fragments arranged by size: 

                2740    1349     895      77
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     374     940    1731    2276    2677    3067    3158 
   Size:        218     156     566     791     545     401     390      91

Cuts at:   3158    4202    4618    5061 
   Size:       1044     416     443

  Fragments arranged by size: 

                1044     791     566     545     443     416     401     390
                 218     156      91
 
AfeI AGC'GCT

Cuts at:      0     508    5061 
   Size:        508    4553
 
AflII C'TTAA_G

Cuts at:      0    3447    3901    5061 
   Size:       3447     454    1160

  Fragments arranged by size: 

                3447    1160     454
 
AflIII A'CryG_T

Cuts at:      0    1370    3322    5061 
   Size:       1370    1952    1739

  Fragments arranged by size: 

                1952    1739    1370
 
AgeI A'CCGG_T

Cuts at:      0    3638    3737    5061 
   Size:       3638      99    1324

  Fragments arranged by size: 

                3638    1324      99
 
AleI CACnn'nnGTG

Cuts at:      0     677    1289    5061 
   Size:        677     612    3772

  Fragments arranged by size: 

                3772     677     612
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2668    3494    5061 
   Size:       2668     826    1567

  Fragments arranged by size: 

                2668    1567     826
 
AlwNI CAG_nnn'CTG

Cuts at:      0     196     241     441    1965    2287    2878    3416    5061 
   Size:        196      45     200    1524     322     591     538    1645

  Fragments arranged by size: 

                1645    1524     591     538     322     200     196      45
            
 
ApaI G_GGCC'C

Cuts at:      0    1861    3500    3979    5061 
   Size:       1861    1639     479    1082

  Fragments arranged by size: 

                1861    1639    1082     479
 
ApaLI G'TGCA_C

Cuts at:      0    1374    1420    4359    4657    4987    5061 
   Size:       1374      46    2939     298     330      74

  Fragments arranged by size: 

                2939    1374     330     298      74      46
 
ApoI r'AATT_y

Cuts at:      0     781    5061 
   Size:        781    4280
 
AvaI C'yCGr_G

Cuts at:      0     137    2605    3385    3928    4166    4615    5061 
   Size:        137    2468     780     543     238     449     446

  Fragments arranged by size: 

                2468     780     543     449     446     238     137
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4504    4537    5061 
   Size:       4504      33     524

  Fragments arranged by size: 

                4504     524      33
 
BamHI G'GATC_C

Cuts at:      0    4499    5013    5061 
   Size:       4499     514      48

  Fragments arranged by size: 

                4499     514      48
 
BanI G'GyrC_C

Cuts at:      0     463    2573    2740    3470    3635    3712    3844    4925 
   Size:        463    2110     167     730     165      77     132    1081

Cuts at:   4925    5061 
   Size:        136

  Fragments arranged by size: 

                2110    1081     730     463     167     165     136     132
                  77
 
BanII G_rGCy'C

Cuts at:      0     127     435    1861    1886    2294    2594    3306    3354 
   Size:        127     308    1426      25     408     300     712      48

Cuts at:   3354    3500    3979    5061 
   Size:        146     479    1082

  Fragments arranged by size: 

                1426    1082     712     479     408     308     300     146
                 127      48      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2004    5061 
   Size:       2004    3057
 
BbvCI CC'TCA_GC

Cuts at:      0     790    2641    5061 
   Size:        790    1851    2420

  Fragments arranged by size: 

                2420    1851     790
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3267    5061 
   Size:       3267    1794
 
BclI T'GATC_A

Cuts at:      0    1491    5061 
   Size:       1491    3570
 
BfrBI ATG'CAT

Cuts at:      0     723    1182    5061 
   Size:        723     459    3879

  Fragments arranged by size: 

                3879     723     459
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1000    1100    3842    5061 
   Size:       1000     100    2742    1219

  Fragments arranged by size: 

                2742    1219    1000     100
 
BlpI GC'TnA_GC

Cuts at:      0     434    1498    1678    5061 
   Size:        434    1064     180    3383

  Fragments arranged by size: 

                3383    1064     434     180
 
Bme1580I G_kGCm'C

Cuts at:      0      64     580    1378    1424    1728    1861    2605    2668 
   Size:         64     516     798      46     304     133     744      63

Cuts at:   2668    3500    3607    3979    4012    4345    4363    4531    4661 
   Size:        832     107     372      33     333      18     168     130

Cuts at:   4661    4991    5061 
   Size:        330      70

  Fragments arranged by size: 

                 832     798     744     516     372     333     330     304
                 168     133     130     107      70      64      63      46
                  33      18
 
BmgBI CAC'GTC

Cuts at:      0    1320    3157    5061 
   Size:       1320    1837    1904

  Fragments arranged by size: 

                1904    1837    1320
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1144    2343    2579    2881    2941    3001    3861    3873 
   Size:       1144    1199     236     302      60      60     860      12

Cuts at:   3873    4433    5061 
   Size:        560     628

  Fragments arranged by size: 

                1199    1144     860     628     560     302     236      60
                  60      12
 
BmtI G_CTAG'C

Cuts at:      0    2621    5061 
   Size:       2621    2440
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3276    5061 
   Size:       3276    1785
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     287     556    1820    2131    2547    3416    5029 
   Size:        278       9     269    1264     311     416     869    1613

Cuts at:   5029    5061 
   Size:         32

  Fragments arranged by size: 

                1613    1264     869     416     311     278     269      32
                   9
 
Bpu10I CC'TnA_GC

Cuts at:      0      65     790    2641    5061 
   Size:         65     725    1851    2420

  Fragments arranged by size: 

                2420    1851     725      65
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     799    2155    2395    3559    4081    4468    5061 
   Size:        601     198    1356     240    1164     522     387     593

  Fragments arranged by size: 

                1356    1164     601     593     522     387     240     198
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     265     462     919    1796    3958    4660    5061 
   Size:        265     197     457     877    2162     702     401

  Fragments arranged by size: 

                2162     877     702     457     401     265     197
 
BsaAI yAC'GTr

Cuts at:      0    3132    4917    5061 
   Size:       3132    1785     144

  Fragments arranged by size: 

                3132    1785     144
 
BsaWI w'CCGG_w

Cuts at:      0    3638    3737    4115    5061 
   Size:       3638      99     378     946

  Fragments arranged by size: 

                3638     946     378      99
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     264     294    1453    1483    2289    2319    2717    2747 
   Size:        264      30    1159      30     806      30     398      30

Cuts at:   2747    3070    3100    5061 
   Size:        323      30    1961

  Fragments arranged by size: 

                1961    1159     806     398     323     264      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     373    1996    2080    2152    2332    2958    3018    3105 
   Size:        373    1623      84      72     180     626      60      87

Cuts at:   3105    3403    4225    5061 
   Size:        298     822     836

  Fragments arranged by size: 

                1623     836     822     626     373     298     180      87
                  84      72      60
 
BseYI C'CCAG_C

Cuts at:      0     276     565     639    1598    1948    2047    2052    3191 
   Size:        276     289      74     959     350      99       5    1139

Cuts at:   3191    3486    3849    4012    4808    5017    5061 
   Size:        295     363     163     796     209      44

  Fragments arranged by size: 

                1139     959     796     363     350     295     289     276
                 209     163      99      74      44       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131    1104    2061    2491    2542    3388    3920    3967 
   Size:        131     973     957     430      51     846     532      47

Cuts at:   3967    4614    5023    5061 
   Size:        647     409      38

  Fragments arranged by size: 

                 973     957     846     647     532     430     409     131
                  51      47      38
 
BsiEI CG_ry'CG

Cuts at:      0     897    4851    5061 
   Size:        897    3954     210

  Fragments arranged by size: 

                3954     897     210
 
BsiHKAI G_wGCw'C

Cuts at:      0     264     542     812    1247    1378    1424    1471    2735 
   Size:        264     278     270     435     131      46      47    1264

Cuts at:   2735    4115    4363    4661    4991    5061 
   Size:       1380     248     298     330      70

  Fragments arranged by size: 

                1380    1264     435     330     298     278     270     264
                 248     131      70      47      46
 
BsiWI C'GTAC_G

Cuts at:      0    4917    5061 
   Size:       4917     144
 
BsmI GAATG_Cn'

Cuts at:      0     699    5061 
   Size:        699    4362
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1310    4354    4486    5061 
   Size:       1310    3044     132     575

  Fragments arranged by size: 

                3044    1310     575     132
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     127     264     435     542     580     812    1247 
   Size:         64      63     137     171     107      38     232     435

Cuts at:   1247    1378    1424    1471    1728    1861    1886    2294    2594 
   Size:        131      46      47     257     133      25     408     300

Cuts at:   2594    2605    2668    2735    3306    3354    3500    3607    3979 
   Size:         11      63      67     571      48     146     107     372

Cuts at:   3979    4012    4115    4345    4363    4531    4661    4991    5061 
   Size:         33     103     230      18     168     130     330      70

  Fragments arranged by size: 

                 571     435     408     372     330     300     257     232
                 230     171     168     146     137     133     131     130
                 107     107     103      70      67      64      63      63
                  48      47      46      38      33      25      18      11
            
 
BspHI T'CATG_A

Cuts at:      0    1188    5061 
   Size:       1188    3873
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     313     551    2370    2660    2790    5000    5061 
   Size:        313     238    1819     290     130    2210      61

  Fragments arranged by size: 

                2210    1819     313     290     238     130      61
 
BsrBI CCG'CTC

Cuts at:      0    4173    5038    5061 
   Size:       4173     865      23

  Fragments arranged by size: 

                4173     865      23
 
BsrDI GCAATG_nn'

Cuts at:      0    3222    4791    5061 
   Size:       3222    1569     270

  Fragments arranged by size: 

                3222    1569     270
 
BsrFI r'CCGG_y

Cuts at:      0    3566    3638    3737    5061 
   Size:       3566      72      99    1324

  Fragments arranged by size: 

                3566    1324      99      72
 
BssSI C'ACGA_G

Cuts at:      0    3580    5061 
   Size:       3580    1481
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1127    1466    1674    2730    3089    3416    3686    3695 
   Size:       1127     339     208    1056     359     327     270       9

Cuts at:   3695    3915    3943    4790    5001    5061 
   Size:        220      28     847     211      60

  Fragments arranged by size: 

                1127    1056     847     359     339     327     270     220
                 211     208      60      28       9
 
BstEII G'GTnAC_C

Cuts at:      0    2029    5061 
   Size:       2029    3032
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3709    5061 
   Size:       3709    1352
 
BstYI r'GATC_y

Cuts at:      0    1196    3368    4499    5013    5061 
   Size:       1196    2172    1131     514      48

  Fragments arranged by size: 

                2172    1196    1131     514      48
 
Bsu36I CC'TnA_GG

Cuts at:      0    1447    1910    2419    5054    5061 
   Size:       1447     463     509    2635       7

  Fragments arranged by size: 

                2635    1447     509     463       7
 
BtgI C'CryG_G

Cuts at:      0      99     930     995    1785    2022    2975    3492    4054 
   Size:         99     831      65     790     237     953     517     562

Cuts at:   4054    4226    5061 
   Size:        172     835

  Fragments arranged by size: 

                 953     835     831     790     562     517     237     172
                  99      65
 
BtsI GCAGTG_nn'

Cuts at:      0     657    1029    1110    1226    2016    2517    2934    3078 
   Size:        657     372      81     116     790     501     417     144

Cuts at:   3078    3405    3425    3440    3476    4272    4648    4711    5061 
   Size:        327      20      15      36     796     376      63     350

  Fragments arranged by size: 

                 796     790     657     501     417     376     372     350
                 327     144     116      81      63      36      20      15
            
 
DraIII CAC_nnn'GTG

Cuts at:      0    1400    3784    4326    4654    5061 
   Size:       1400    2384     542     328     407

  Fragments arranged by size: 

                2384    1400     542     407     328
 
EaeI y'GGCC_r

Cuts at:      0     713    3675    4257    5061 
   Size:        713    2962     582     804

  Fragments arranged by size: 

                2962     804     713     582
 
EarI CTCTTCn'nnn_

Cuts at:      0     194     350     371     376     481    1103    2852    5061 
   Size:        194     156      21       5     105     622    1749    2209

  Fragments arranged by size: 

                2209    1749     622     194     156     105      21       5
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     775    5061 
   Size:        775    4286
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     278     287     374     556     940    1731    1820 
   Size:        218      60       9      87     182     384     791      89

Cuts at:   1820    2131    2276    2547    2677    3067    3158    3416    4202 
   Size:        311     145     271     130     390      91     258     786

Cuts at:   4202    4618    5029    5061 
   Size:        416     411      32

  Fragments arranged by size: 

                 791     786     416     411     390     384     311     271
                 258     218     182     145     130      91      89      87
                  60      32       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     793    2137    2897    2957    3017    5061 
   Size:        793    1344     760      60      60    2044

  Fragments arranged by size: 

                2044    1344     793     760      60      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112     141     798    1443    1857    1962    2094    2171 
   Size:        112      29     657     645     414     105     132      77

Cuts at:   2171    2415    2547    2922    2982    3042    3179    3497    3975 
   Size:        244     132     375      60      60     137     318     478

Cuts at:   3975    3976    4070    5061 
   Size:          1      94     991

  Fragments arranged by size: 

                 991     657     645     478     414     375     318     244
                 137     132     132     112     105      94      77      60
                  60      29       1
 
EcoRI G'AATT_C

Cuts at:      0     781    5061 
   Size:        781    4280
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     528    2881    2955    3075    4746    5061 
   Size:        528    2353      74     120    1671     315

  Fragments arranged by size: 

                2353    1671     528     315     120      74
 
FspI TGC'GCA

Cuts at:      0    4741    5061 
   Size:       4741     320
 
HaeII r_GCGC'y

Cuts at:      0     510    5061 
   Size:        510    4551
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     114     146     264     296     442     474    1170    1202 
   Size:        114      32     118      32     146      32     696      32

Cuts at:   1202    2098    2130    2386    2418    4382    4414    4659    4691 
   Size:        896      32     256      32    1964      32     245      32

Cuts at:   4691    5061 
   Size:        370

  Fragments arranged by size: 

                1964     896     696     370     256     245     146     118
                 114      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1989    3828    4560    5061 
   Size:       1989    1839     732     501

  Fragments arranged by size: 

                1989    1839     732     501
 
KpnI G_GTAC'C

Cuts at:      0    2744    3639    3716    5061 
   Size:       2744     895      77    1345

  Fragments arranged by size: 

                2744    1345     895      77
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     761    1275    1379    5061 
   Size:        761     514     104    3682

  Fragments arranged by size: 

                3682     761     514     104
 
MscI TGG'CCA

Cuts at:      0     715    4259    5061 
   Size:        715    3544     802

  Fragments arranged by size: 

                3544     802     715
 
MslI CAynn'nnrTG

Cuts at:      0     677    1187    1271    1289    1790    2027    4608    4994 
   Size:        677     510      84      18     501     237    2581     386

Cuts at:   4994    5061 
   Size:         67

  Fragments arranged by size: 

                2581     677     510     501     386     237      84      67
                  18
 
MspA1I CmG'CkG

Cuts at:      0     352     438    1497    1896    2075    2510    3833    3995 
   Size:        352      86    1059     399     179     435    1323     162

Cuts at:   3995    4142    4308    4566    4808    5007    5061 
   Size:        147     166     258     242     199      54

  Fragments arranged by size: 

                1323    1059     435     399     352     258     242     199
                 179     166     162     147      86      54
 
NcoI C'CATG_G

Cuts at:      0     930     995    1785    2022    5061 
   Size:        930      65     790     237    3039

  Fragments arranged by size: 

                3039     930     790     237      65
 
NdeI CA'TA_TG

Cuts at:      0     488    5061 
   Size:        488    4573
 
NheI G'CTAG_C

Cuts at:      0    2617    5061 
   Size:       2617    2444
 
NsiI A_TGCA'T

Cuts at:      0     725    1184    5061 
   Size:        725     459    3877

  Fragments arranged by size: 

                3877     725     459
 
NspI r_CATG'y

Cuts at:      0     723    1186    1260    1313    1344    1374    1428    1438 
   Size:        723     463      74      53      31      30      54      10

Cuts at:   1438    3326    3558    3913    4298    4497    5061 
   Size:       1888     232     355     385     199     564

  Fragments arranged by size: 

                1888     723     564     463     385     355     232     199
                  74      54      53      31      30      10
 
PasI CC'CwG_GG

Cuts at:      0    2097    2175    2837    5061 
   Size:       2097      78     662    2224

  Fragments arranged by size: 

                2224    2097     662      78
 
PciI A'CATG_T

Cuts at:      0    1370    3322    5061 
   Size:       1370    1952    1739

  Fragments arranged by size: 

                1952    1739    1370
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3493    4308    4959    5061 
   Size:       3493     815     651     102

  Fragments arranged by size: 

                3493     815     651     102
 
PfoI T'CCnGG_A

Cuts at:      0     264     294    2563    5061 
   Size:        264      30    2269    2498

  Fragments arranged by size: 

                2498    2269     264      30
 
PmlI CAC'GTG

Cuts at:      0    3132    5061 
   Size:       3132    1929
 
PpuMI rG'GwC_Cy

Cuts at:      0     141    1443    3179    5061 
   Size:        141    1302    1736    1882

  Fragments arranged by size: 

                1882    1736    1302     141
 
PspOMI G'GGCC_C

Cuts at:      0    1857    3496    3975    5061 
   Size:       1857    1639     479    1086

  Fragments arranged by size: 

                1857    1639    1086     479
 
PspXI vC'TCGA_Gb

Cuts at:      0    3385    5061 
   Size:       3385    1676
 
PstI C_TGCA'G

Cuts at:      0     880    1583    1648    2901    2961    3021    3148    3412 
   Size:        880     703      65    1253      60      60     127     264

Cuts at:   3412    3483    3691    3963    5061 
   Size:         71     208     272    1098

  Fragments arranged by size: 

                1253    1098     880     703     272     264     208     127
                  71      65      60      60
 
PvuII CAG'CTG

Cuts at:      0     352    1497    1896    2075    3833    4308    4566    5007 
   Size:        352    1145     399     179    1758     475     258     441

Cuts at:   5007    5061 
   Size:         54

  Fragments arranged by size: 

                1758    1145     475     441     399     352     258     179
                  54
 
SanDI GG'GwC_CC

Cuts at:      0     141    3179    5061 
   Size:        141    3038    1882

  Fragments arranged by size: 

                3038    1882     141
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1103    2852    5061 
   Size:       1103    1749    2209

  Fragments arranged by size: 

                2209    1749    1103
 
SbfI CC_TGCA'GG

Cuts at:      0    2901    2961    3021    5061 
   Size:       2901      60      60    2040

  Fragments arranged by size: 

                2901    2040      60      60
 
ScaI AGT'ACT

Cuts at:      0    4627    5061 
   Size:       4627     434
 
SfcI C'TryA_G

Cuts at:      0     675     876    1579    1644    2897    2957    3017    3144 
   Size:        675     201     703      65    1253      60      60     127

Cuts at:   3144    3408    3479    3511    3687    3789    3959    5061 
   Size:        264      71      32     176     102     170    1102

  Fragments arranged by size: 

                1253    1102     703     675     264     201     176     170
                 127     102      71      65      60      60      32
 
SgrAI Cr'CCGG_yG

Cuts at:      0    3566    5061 
   Size:       3566    1495
 
SmaI CCC'GGG

Cuts at:      0     139    4168    5061 
   Size:        139    4029     893

  Fragments arranged by size: 

                4029     893     139
 
SmlI C'TyrA_G

Cuts at:      0     616     814    2134    2410    3385    3447    3574    3901 
   Size:        616     198    1320     276     975      62     127     327

Cuts at:   3901    4060    4483    5061 
   Size:        159     423     578

  Fragments arranged by size: 

                1320     975     616     578     423     327     276     198
                 159     127      62
 
SphI G_CATG'C

Cuts at:      0     723    1186    3558    3913    5061 
   Size:        723     463    2372     355    1148

  Fragments arranged by size: 

                2372    1148     723     463     355
 
StuI AGG'CCT

Cuts at:      0     904    1810    2749    4084    4717    5061 
   Size:        904     906     939    1335     633     344

  Fragments arranged by size: 

                1335     939     906     904     633     344
 
StyI C'CwwG_G

Cuts at:      0     426     930     995    1361    1785    2022    2577    3840 
   Size:        426     504      65     366     424     237     555    1263

Cuts at:   3840    5061 
   Size:       1221

  Fragments arranged by size: 

                1263    1221     555     504     426     424     366     237
                  65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     406     631    2026    2239    2561    2662    2727    2988 
   Size:        406     225    1395     213     322     101      65     261

Cuts at:   2988    3048    4067    4759    4855    5061 
   Size:         60    1019     692      96     206

  Fragments arranged by size: 

                1395    1019     692     406     322     261     225     213
                 206     101      96      65      60
 
TatI w'GTAC_w

Cuts at:      0    3247    4625    5061 
   Size:       3247    1378     436

  Fragments arranged by size: 

                3247    1378     436
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      43      75    1357    1389    1579    1611    1751    1783 
   Size:         43      32    1282      32     190      32     140      32

Cuts at:   1783    2289    2321    3070    3102    5061 
   Size:        506      32     749      32    1959

  Fragments arranged by size: 

                1959    1282     749     506     190     140      43      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2947    3007    5061 
   Size:       2947      60    2054

  Fragments arranged by size: 

                2947    2054      60
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1606    3514    5061 
   Size:       1606    1908    1547

  Fragments arranged by size: 

                1908    1606    1547
 
XhoI C'TCGA_G

Cuts at:      0    3385    5061 
   Size:       3385    1676
 
XmaI C'CCGG_G

Cuts at:      0     137    4166    5061 
   Size:        137    4029     895

  Fragments arranged by size: 

                4029     895     137
 
XmnI GAAnn'nnTTC

Cuts at:      0     595    5061 
   Size:        595    4466
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PstI    PvuII    SanDI     SapI     SbfI     ScaI
     SfcI    SgrAI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AhdI     AloI     AscI     AseI    AsiSI
    AvrII     BbeI     BcgI    BglII    BsaBI    BsaHI    BspEI    BsrGI
   BssHII    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI  EcoICRI    EcoRV     FseI    FspAI  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI   NgoMIV     NotI     NruI     PacI
     PmeI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SexAI     SfiI     SfoI     SgfI    SnaBI     SpeI
     SrfI     SspI     SwaI     XbaI     ZraI
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