Restriction map for
pF1KSDA0238
(Linear) (Six-base) MAPSORT of: KIAA0238.seq Check: 4922 from: 1 to: 1950
KIAA0238.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
Acc65I G'GTAC_C
Cuts at: 0 296 1950
Size: 296 1654
AclI AA'CG_TT
Cuts at: 0 469 1950
Size: 469 1481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 736 1830 1950
Size: 324 412 1094 120
Fragments arranged by size:
1094 412 324 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 1950
Size: 835 1115
ApaLI G'TGCA_C
Cuts at: 0 1202 1950
Size: 1202 748
ApoI r'AATT_y
Cuts at: 0 871 1253 1533 1950
Size: 871 382 280 417
Fragments arranged by size:
871 417 382 280
AvaI C'yCGr_G
Cuts at: 0 527 1950
Size: 527 1423
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 288 321 1950
Size: 288 33 1629
Fragments arranged by size:
1629 288 33
BamHI G'GATC_C
Cuts at: 0 632 1449 1950
Size: 632 817 501
Fragments arranged by size:
817 632 501
BanI G'GyrC_C
Cuts at: 0 124 296 1950
Size: 124 172 1654
Fragments arranged by size:
1654 172 124
BanII G_rGCy'C
Cuts at: 0 167 211 711 1467 1583 1950
Size: 167 44 500 756 116 367
Fragments arranged by size:
756 500 367 167 116 44
BbeI G_GCGC'C
Cuts at: 0 128 1950
Size: 128 1822
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 996 1937 1950
Size: 77 919 941 13
Fragments arranged by size:
941 919 77 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 339 373 1950
Size: 339 34 1577
Fragments arranged by size:
1577 339 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 635 975 1950
Size: 635 340 975
Fragments arranged by size:
975 635 340
BclI T'GATC_A
Cuts at: 0 1490 1950
Size: 1490 460
BglI GCCn_nnn'nGGC
Cuts at: 0 695 1950
Size: 695 1255
Bme1580I G_kGCm'C
Cuts at: 0 362 1206 1221 1950
Size: 362 844 15 729
Fragments arranged by size:
844 729 362 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 816 1310 1950
Size: 816 494 640
Fragments arranged by size:
816 640 494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1408 1950
Size: 1408 542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1709 1950
Size: 117 1592 241
Fragments arranged by size:
1592 241 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 1950
Size: 214 1736
BsaHI Gr'CG_yC
Cuts at: 0 125 1950
Size: 125 1825
BsaWI w'CCGG_w
Cuts at: 0 105 1446 1742 1950
Size: 105 1341 296 208
Fragments arranged by size:
1341 296 208 105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 1950
Size: 682 1268
BseYI C'CCAG_C
Cuts at: 0 87 251 1458 1950
Size: 87 164 1207 492
Fragments arranged by size:
1207 492 164 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1458 1950
Size: 1458 492
BsiEI CG_ry'CG
Cuts at: 0 353 1133 1950
Size: 353 780 817
Fragments arranged by size:
817 780 353
BsiHKAI G_wGCw'C
Cuts at: 0 167 211 1206 1950
Size: 167 44 995 744
Fragments arranged by size:
995 744 167 44
Bsp1286I G_dGCh'C
Cuts at: 0 167 211 362 711 1206 1221 1467 1583
Size: 167 44 151 349 495 15 246 116
Cuts at: 1583 1950
Size: 367
Fragments arranged by size:
495 367 349 246 167 151 116 44
15
BspEI T'CCGG_A
Cuts at: 0 1446 1742 1950
Size: 1446 296 208
Fragments arranged by size:
1446 296 208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 1523 1950
Size: 470 1053 427
Fragments arranged by size:
1053 470 427
BsrDI GCAATG_nn'
Cuts at: 0 194 812 1950
Size: 194 618 1138
Fragments arranged by size:
1138 618 194
BsrFI r'CCGG_y
Cuts at: 0 1132 1950
Size: 1132 818
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1062 1950
Size: 1062 888
BstEII G'GTnAC_C
Cuts at: 0 482 1950
Size: 482 1468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 387 421 1106 1950
Size: 36 351 34 685 844
Fragments arranged by size:
844 685 351 36 34
BstYI r'GATC_y
Cuts at: 0 632 641 1449 1950
Size: 632 9 808 501
Fragments arranged by size:
808 632 501 9
Bsu36I CC'TnA_GG
Cuts at: 0 1897 1950
Size: 1897 53
BtgI C'CryG_G
Cuts at: 0 746 1078 1157 1943 1950
Size: 746 332 79 786 7
Fragments arranged by size:
786 746 332 79 7
BtsI GCAGTG_nn'
Cuts at: 0 362 1950
Size: 362 1588
ClaI AT'CG_AT
Cuts at: 0 1637 1950
Size: 1637 313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1856 1891 1950
Size: 1856 35 59
Fragments arranged by size:
1856 59 35
DraI TTT'AAA
Cuts at: 0 1532 1950
Size: 1532 418
EaeI y'GGCC_r
Cuts at: 0 953 1130 1950
Size: 953 177 820
Fragments arranged by size:
953 820 177
EagI C'GGCC_G
Cuts at: 0 1130 1950
Size: 1130 820
EarI CTCTTCn'nnn_
Cuts at: 0 107 1897 1950
Size: 107 1790 53
Fragments arranged by size:
1790 107 53
EcoICRI GAG'CTC
Cuts at: 0 165 209 1950
Size: 165 44 1741
Fragments arranged by size:
1741 165 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 324 736 1709 1830 1950
Size: 117 207 412 973 121 120
Fragments arranged by size:
973 412 207 121 120 117
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 1950
Size: 493 1457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1047 1081 1950
Size: 1047 34 869
Fragments arranged by size:
1047 869 34
HaeII r_GCGC'y
Cuts at: 0 128 1430 1950
Size: 128 1302 520
Fragments arranged by size:
1302 520 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1553 1585 1918 1950
Size: 1553 32 333 32
Fragments arranged by size:
1553 333 32 32
KasI G'GCGC_C
Cuts at: 0 124 1950
Size: 124 1826
KpnI G_GTAC'C
Cuts at: 0 300 1950
Size: 300 1650
MscI TGG'CCA
Cuts at: 0 955 1950
Size: 955 995
MslI CAynn'nnrTG
Cuts at: 0 385 658 817 1104 1144 1950
Size: 385 273 159 287 40 806
Fragments arranged by size:
806 385 287 273 159 40
MspA1I CmG'CkG
Cuts at: 0 137 344 784 927 1498 1950
Size: 137 207 440 143 571 452
Fragments arranged by size:
571 452 440 207 143 137
NarI GG'CG_CC
Cuts at: 0 125 1950
Size: 125 1825
NspI r_CATG'y
Cuts at: 0 338 1149 1950
Size: 338 811 801
Fragments arranged by size:
811 801 338
PfoI T'CCnGG_A
Cuts at: 0 1540 1700 1950
Size: 1540 160 250
Fragments arranged by size:
1540 250 160
PshAI GACnn'nnGTC
Cuts at: 0 1000 1950
Size: 1000 950
PsiI TTA'TAA
Cuts at: 0 890 1828 1950
Size: 890 938 122
Fragments arranged by size:
938 890 122
PspXI vC'TCGA_Gb
Cuts at: 0 527 1950
Size: 527 1423
PstI C_TGCA'G
Cuts at: 0 1520 1950
Size: 1520 430
PvuI CG_AT'CG
Cuts at: 0 353 1950
Size: 353 1597
PvuII CAG'CTG
Cuts at: 0 927 1498 1950
Size: 927 571 452
Fragments arranged by size:
927 571 452
SacI G_AGCT'C
Cuts at: 0 167 211 1950
Size: 167 44 1739
Fragments arranged by size:
1739 167 44
SapI GCTCTTCn'nnn_
Cuts at: 0 1897 1950
Size: 1897 53
SfcI C'TryA_G
Cuts at: 0 319 460 1516 1950
Size: 319 141 1056 434
Fragments arranged by size:
1056 434 319 141
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 695 1950
Size: 695 1255
SfoI GGC'GCC
Cuts at: 0 126 1950
Size: 126 1824
SmlI C'TyrA_G
Cuts at: 0 527 1950
Size: 527 1423
SphI G_CATG'C
Cuts at: 0 1149 1950
Size: 1149 801
SspI AAT'ATT
Cuts at: 0 830 1950
Size: 830 1120
StuI AGG'CCT
Cuts at: 0 1268 1895 1950
Size: 1268 627 55
Fragments arranged by size:
1268 627 55
SwaI ATTT'AAAT
Cuts at: 0 1532 1950
Size: 1532 418
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 715 759 1950
Size: 101 614 44 1191
Fragments arranged by size:
1191 614 101 44
TatI w'GTAC_w
Cuts at: 0 719 1950
Size: 719 1231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 1743 1775 1950
Size: 1244 32 467 32 175
Fragments arranged by size:
1244 467 175 32 32
XhoI C'TCGA_G
Cuts at: 0 527 1950
Size: 527 1423
XmnI GAAnn'nnTTC
Cuts at: 0 284 1950
Size: 284 1666
Enzymes that do cut:
Acc65I AclI AcuI AlfI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI Bme1580I BmrI BplI BpmI BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI ClaI CspCI DraI EaeI EagI EarI EcoICRI
Eco57MI EcoNI FalI HaeII Hin4I KasI KpnI MscI
MslI MspA1I NarI NspI PfoI PshAI PsiI PspXI
PstI PvuI PvuII SacI SapI SfcI SfiI SfoI
SmlI SphI SspI StuI SwaI TaqII TatI TstI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BbvCI BfrBI BglII BlpI BmgBI BmtI Bpu10I BpuEI
BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I BtgZI DraIII DrdI
EciI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PsrI RsrII
SacII SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SmaI SnaBI SpeI SrfI StyI Tth111I XbaI XcmI
XmaI ZraI