Restriction map for
pF1KA0244
(Linear) (Six-base) MAPSORT of: KIAA0244.seq Check: 6904 from: 1 to: 6117
KIAA0244.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 2638 3595 5975 6117
Size: 2638 957 2380 142
Fragments arranged by size:
2638 2380 957 142
Acc65I G'GTAC_C
Cuts at: 0 627 2847 6071 6117
Size: 627 2220 3224 46
Fragments arranged by size:
3224 2220 627 46
AclI AA'CG_TT
Cuts at: 0 3544 6117
Size: 3544 2573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 644 653 1384 2333 3854 3858 4072
Size: 154 490 9 731 949 1521 4 214
Cuts at: 4072 4234 4774 4976 5398 6117
Size: 162 540 202 422 719
Fragments arranged by size:
1521 949 731 719 540 490 422 214
202 162 154 9 4
AfeI AGC'GCT
Cuts at: 0 3889 6117
Size: 3889 2228
AflII C'TTAA_G
Cuts at: 0 339 6117
Size: 339 5778
AflIII A'CryG_T
Cuts at: 0 1851 5308 6117
Size: 1851 3457 809
Fragments arranged by size:
3457 1851 809
AhdI GACnn_n'nnGTC
Cuts at: 0 3300 6117
Size: 3300 2817
AlwNI CAG_nnn'CTG
Cuts at: 0 3685 3832 4050 4070 5724 6117
Size: 3685 147 218 20 1654 393
Fragments arranged by size:
3685 1654 393 218 147 20
ApaLI G'TGCA_C
Cuts at: 0 4109 5251 6117
Size: 4109 1142 866
Fragments arranged by size:
4109 1142 866
ApoI r'AATT_y
Cuts at: 0 360 1338 1617 1760 2374 2483 2514 2838
Size: 360 978 279 143 614 109 31 324
Cuts at: 2838 3505 3658 3803 3845 3962 4484 4722 5320
Size: 667 153 145 42 117 522 238 598
Cuts at: 5320 5646 6117
Size: 326 471
Fragments arranged by size:
978 667 614 598 522 471 360 326
324 279 238 153 145 143 117 109
42 31
AvrII C'CTAG_G
Cuts at: 0 4954 6117
Size: 4954 1163
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 559 592 1583 1616 6117
Size: 559 33 991 33 4501
Fragments arranged by size:
4501 991 559 33 33
BamHI G'GATC_C
Cuts at: 0 67 156 4950 6117
Size: 67 89 4794 1167
Fragments arranged by size:
4794 1167 89 67
BanI G'GyrC_C
Cuts at: 0 627 2847 3375 3949 4423 5684 6071 6117
Size: 627 2220 528 574 474 1261 387 46
Fragments arranged by size:
2220 1261 627 574 528 474 387 46
BbeI G_GCGC'C
Cuts at: 0 5688 6117
Size: 5688 429
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 638 2285 2324 4262 6011 6117
Size: 496 142 1647 39 1938 1749 106
Fragments arranged by size:
1938 1749 1647 496 142 106 39
BbvCI CC'TCA_GC
Cuts at: 0 1889 4238 6117
Size: 1889 2349 1879
Fragments arranged by size:
2349 1889 1879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5242 5276 6117
Size: 5242 34 841
Fragments arranged by size:
5242 841 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1128 6117
Size: 1128 4989
BclI T'GATC_A
Cuts at: 0 2106 3021 6117
Size: 2106 915 3096
Fragments arranged by size:
3096 2106 915
BfrBI ATG'CAT
Cuts at: 0 3472 6117
Size: 3472 2645
BglI GCCn_nnn'nGGC
Cuts at: 0 4421 5682 6117
Size: 4421 1261 435
Fragments arranged by size:
4421 1261 435
BglII A'GATC_T
Cuts at: 0 370 4849 6117
Size: 370 4479 1268
Fragments arranged by size:
4479 1268 370
BlpI GC'TnA_GC
Cuts at: 0 145 6117
Size: 145 5972
Bme1580I G_kGCm'C
Cuts at: 0 4113 4426 5255 5897 5916 6117
Size: 4113 313 829 642 19 201
Fragments arranged by size:
4113 829 642 313 201 19
BmgBI CAC'GTC
Cuts at: 0 3562 6117
Size: 3562 2555
BmrI ACTGGGnnnn_n'
Cuts at: 0 4446 6117
Size: 4446 1671
BmtI G_CTAG'C
Cuts at: 0 2560 6117
Size: 2560 3557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2916 3312 5136 6117
Size: 2916 396 1824 981
Fragments arranged by size:
2916 1824 981 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 5510 6117
Size: 97 5413 607
Fragments arranged by size:
5413 607 97
Bpu10I CC'TnA_GC
Cuts at: 0 1889 4238 6117
Size: 1889 2349 1879
Fragments arranged by size:
2349 1889 1879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 2240 5177 5407 6117
Size: 1027 1213 2937 230 710
Fragments arranged by size:
2937 1213 1027 710 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 4928 6117
Size: 4928 1189
BsaAI yAC'GTr
Cuts at: 0 1926 6117
Size: 1926 4191
BsaBI GATnn'nnATC
Cuts at: 0 155 6117
Size: 155 5962
BsaHI Gr'CG_yC
Cuts at: 0 5685 6117
Size: 5685 432
BsaWI w'CCGG_w
Cuts at: 0 1290 6117
Size: 1290 4827
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1497 1527 3534 3564 6117
Size: 1497 30 2007 30 2553
Fragments arranged by size:
2553 2007 1497 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2106 2552 3301 3529 3532 6117
Size: 2106 446 749 228 3 2585
Fragments arranged by size:
2585 2106 749 446 228 3
BseYI C'CCAG_C
Cuts at: 0 1902 2146 4346 6117
Size: 1902 244 2200 1771
Fragments arranged by size:
2200 1902 1771 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1823 6117
Size: 618 1205 4294
Fragments arranged by size:
4294 1205 618
BsiHKAI G_wGCw'C
Cuts at: 0 433 1240 4113 5069 5255 6117
Size: 433 807 2873 956 186 862
Fragments arranged by size:
2873 956 862 807 433 186
BsmI GAATG_Cn'
Cuts at: 0 1359 6117
Size: 1359 4758
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3072 6117
Size: 3072 3045
Bsp1286I G_dGCh'C
Cuts at: 0 433 1240 4113 4426 5069 5255 5897 5916
Size: 433 807 2873 313 643 186 642 19
Cuts at: 5916 6117
Size: 201
Fragments arranged by size:
2873 807 643 642 433 313 201 186
19
BspHI T'CATG_A
Cuts at: 0 750 2685 6117
Size: 750 1935 3432
Fragments arranged by size:
3432 1935 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3909 6117
Size: 3909 2208
BsrDI GCAATG_nn'
Cuts at: 0 216 300 1913 4123 6117
Size: 216 84 1613 2210 1994
Fragments arranged by size:
2210 1994 1613 216 84
BsrGI T'GTAC_A
Cuts at: 0 1048 2736 6117
Size: 1048 1688 3381
Fragments arranged by size:
3381 1688 1048
BssSI C'ACGA_G
Cuts at: 0 5121 6117
Size: 5121 996
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2872 3359 6117
Size: 2872 487 2758
Fragments arranged by size:
2872 2758 487
BstBI TT'CG_AA
Cuts at: 0 5262 6117
Size: 5262 855
BstYI r'GATC_y
Cuts at: 0 67 156 169 370 1018 2340 2599 2967
Size: 67 89 13 201 648 1322 259 368
Cuts at: 2967 3268 4849 4950 6117
Size: 301 1581 101 1167
Fragments arranged by size:
1581 1322 1167 648 368 301 259 201
101 89 67 13
BtgI C'CryG_G
Cuts at: 0 1139 3218 5552 6117
Size: 1139 2079 2334 565
Fragments arranged by size:
2334 2079 1139 565
BtsI GCAGTG_nn'
Cuts at: 0 2161 6117
Size: 2161 3956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2139 2174 2865 2900 5483 5518 6117
Size: 2139 35 691 35 2583 35 599
Fragments arranged by size:
2583 2139 691 599 35 35 35
DraI TTT'AAA
Cuts at: 0 2489 2991 4496 5147 6117
Size: 2489 502 1505 651 970
Fragments arranged by size:
2489 1505 970 651 502
DraIII CAC_nnn'GTG
Cuts at: 0 5929 6117
Size: 5929 188
EaeI y'GGCC_r
Cuts at: 0 3142 5563 6117
Size: 3142 2421 554
Fragments arranged by size:
3142 2421 554
EarI CTCTTCn'nnn_
Cuts at: 0 1928 1964 2088 2570 2815 3078 4394 4647
Size: 1928 36 124 482 245 263 1316 253
Cuts at: 4647 5259 6117
Size: 612 858
Fragments arranged by size:
1928 1316 858 612 482 263 253 245
124 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 154 644 653 1384 2333 3854 3858
Size: 97 57 490 9 731 949 1521 4
Cuts at: 3858 4072 4234 4774 4976 5398 5510 6117
Size: 214 162 540 202 422 112 607
Fragments arranged by size:
1521 949 731 607 540 490 422 214
202 162 112 97 57 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 164 3706 6117
Size: 164 3542 2411
Fragments arranged by size:
3542 2411 164
EcoO109I rG'GnC_Cy
Cuts at: 0 1899 3711 4637 5455 5608 6117
Size: 1899 1812 926 818 153 509
Fragments arranged by size:
1899 1812 926 818 509 153
EcoRI G'AATT_C
Cuts at: 0 2374 3505 6117
Size: 2374 1131 2612
Fragments arranged by size:
2612 2374 1131
EcoRV GAT'ATC
Cuts at: 0 3746 6117
Size: 3746 2371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 674 2060 2746 2830 5085 6117
Size: 674 1386 686 84 2255 1032
Fragments arranged by size:
2255 1386 1032 686 674 84
HaeII r_GCGC'y
Cuts at: 0 3891 5688 6117
Size: 3891 1797 429
Fragments arranged by size:
3891 1797 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 1003 1035 1201 1233 2098 2130
Size: 730 32 241 32 166 32 865 32
Cuts at: 2130 2786 2818 3131 3163 3286 3318 3460 3492
Size: 656 32 313 32 123 32 142 32
Cuts at: 3492 3735 3767 4659 4691 5595 5627 5809 5841
Size: 243 32 892 32 904 32 182 32
Cuts at: 5841 6117
Size: 276
Fragments arranged by size:
904 892 865 730 656 313 276 243
241 182 166 142 123 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3294 3322 3542 6117
Size: 3294 28 220 2575
Fragments arranged by size:
3294 2575 220 28
HindIII A'AGCT_T
Cuts at: 0 812 2410 2666 6117
Size: 812 1598 256 3451
Fragments arranged by size:
3451 1598 812 256
KasI G'GCGC_C
Cuts at: 0 5684 6117
Size: 5684 433
KpnI G_GTAC'C
Cuts at: 0 631 2851 6075 6117
Size: 631 2220 3224 42
Fragments arranged by size:
3224 2220 631 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 1211 4288 5280 6117
Size: 95 1116 3077 992 837
Fragments arranged by size:
3077 1116 992 837 95
MscI TGG'CCA
Cuts at: 0 5565 6117
Size: 5565 552
MslI CAynn'nnrTG
Cuts at: 0 47 992 2815 3106 3167 6117
Size: 47 945 1823 291 61 2950
Fragments arranged by size:
2950 1823 945 291 61 47
NarI GG'CG_CC
Cuts at: 0 5685 6117
Size: 5685 432
NcoI C'CATG_G
Cuts at: 0 1139 3218 5552 6117
Size: 1139 2079 2334 565
Fragments arranged by size:
2334 2079 1139 565
NheI G'CTAG_C
Cuts at: 0 2556 6117
Size: 2556 3561
NsiI A_TGCA'T
Cuts at: 0 3474 6117
Size: 3474 2643
NspI r_CATG'y
Cuts at: 0 144 1855 3645 5040 5312 5484 6117
Size: 144 1711 1790 1395 272 172 633
Fragments arranged by size:
1790 1711 1395 633 272 172 144
PciI A'CATG_T
Cuts at: 0 1851 5308 6117
Size: 1851 3457 809
Fragments arranged by size:
3457 1851 809
PflMI CCAn_nnn'nTGG
Cuts at: 0 3685 5533 6117
Size: 3685 1848 584
Fragments arranged by size:
3685 1848 584
PfoI T'CCnGG_A
Cuts at: 0 726 6117
Size: 726 5391
PmlI CAC'GTG
Cuts at: 0 1926 6117
Size: 1926 4191
PpuMI rG'GwC_Cy
Cuts at: 0 3711 4637 5608 6117
Size: 3711 926 971 509
Fragments arranged by size:
3711 971 926 509
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 308 340 6117
Size: 308 32 5777
Fragments arranged by size:
5777 308 32
PstI C_TGCA'G
Cuts at: 0 2772 4656 5112 6117
Size: 2772 1884 456 1005
Fragments arranged by size:
2772 1884 1005 456
SapI GCTCTTCn'nnn_
Cuts at: 0 1928 6117
Size: 1928 4189
ScaI AGT'ACT
Cuts at: 0 1281 1368 2999 4080 6117
Size: 1281 87 1631 1081 2037
Fragments arranged by size:
2037 1631 1281 1081 87
SexAI A'CCwGG_T
Cuts at: 0 2676 5654 6117
Size: 2676 2978 463
Fragments arranged by size:
2978 2676 463
SfcI C'TryA_G
Cuts at: 0 1358 2366 2526 2768 3383 4160 4598 4652
Size: 1358 1008 160 242 615 777 438 54
Cuts at: 4652 4703 4890 5108 6117
Size: 51 187 218 1009
Fragments arranged by size:
1358 1009 1008 777 615 438 242 218
187 160 54 51
SfoI GGC'GCC
Cuts at: 0 5686 6117
Size: 5686 431
SmlI C'TyrA_G
Cuts at: 0 339 1006 2255 5192 5422 6117
Size: 339 667 1249 2937 230 695
Fragments arranged by size:
2937 1249 695 667 339 230
SpeI A'CTAG_T
Cuts at: 0 1276 3316 4081 6117
Size: 1276 2040 765 2036
Fragments arranged by size:
2040 2036 1276 765
SspI AAT'ATT
Cuts at: 0 3492 4587 5241 6117
Size: 3492 1095 654 876
Fragments arranged by size:
3492 1095 876 654
StyI C'CwwG_G
Cuts at: 0 1139 3218 3797 4954 5552 5698 6117
Size: 1139 2079 579 1157 598 146 419
Fragments arranged by size:
2079 1157 1139 598 579 419 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1961 3937 5270 5403 6117
Size: 1961 1976 1333 133 714
Fragments arranged by size:
1976 1961 1333 714 133
TatI w'GTAC_w
Cuts at: 0 1048 1279 1366 1722 1755 2736 2997 4078
Size: 1048 231 87 356 33 981 261 1081
Cuts at: 4078 5374 6117
Size: 1296 743
Fragments arranged by size:
1296 1081 1048 981 743 356 261 231
87 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1497 1529 5086 5118 6117
Size: 1497 32 3557 32 999
Fragments arranged by size:
3557 1497 999 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4973 6117
Size: 4973 1144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3249 6117
Size: 3249 2868
XmnI GAAnn'nnTTC
Cuts at: 0 4259 6117
Size: 4259 1858
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaLI ApoI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstBI BstYI BtgI BtsI
CspCI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI NarI NcoI NheI NsiI
NspI PciI PflMI PfoI PmlI PpuMI PsrI PstI
SapI ScaI SexAI SfcI SfoI SmlI SpeI SspI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AgeI AleI AlfI AloI ApaI AscI
AseI AsiSI AvaI BanII BsiEI BsiWI BspEI BsrBI
BsrFI BssHII BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DrdI EagI EciI EcoICRI FseI FspI FspAI HpaI
MfeI MluI MspA1I NaeI NdeI NgoMIV NotI NruI
PacI PasI PmeI PpiI PshAI PsiI PspOMI PspXI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SfiI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI XbaI XhoI XmaI ZraI