Restriction map for
pF1KA0249
(Linear) (Six-base) MAPSORT of: KIAA0249.seq Check: 4434 from: 1 to: 2688
KIAA0249.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 18 2688
Size: 18 2670
AccI GT'mk_AC
Cuts at: 0 1145 2688
Size: 1145 1543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 350 1259 1966 2684 2688
Size: 296 54 909 707 718 4
Fragments arranged by size:
909 718 707 296 54 4
AflIII A'CryG_T
Cuts at: 0 414 538 2688
Size: 414 124 2150
Fragments arranged by size:
2150 414 124
AhdI GACnn_n'nnGTC
Cuts at: 0 716 1152 2688
Size: 716 436 1536
Fragments arranged by size:
1536 716 436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2287 2688
Size: 2287 401
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2563 2595 2688
Size: 2563 32 93
Fragments arranged by size:
2563 93 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1384 2688
Size: 1384 1304
ApaI G_GGCC'C
Cuts at: 0 579 2284 2688
Size: 579 1705 404
Fragments arranged by size:
1705 579 404
ApoI r'AATT_y
Cuts at: 0 1445 1459 1489 2604 2688
Size: 1445 14 30 1115 84
Fragments arranged by size:
1445 1115 84 30 14
AseI AT'TA_AT
Cuts at: 0 2512 2688
Size: 2512 176
AvaI C'yCGr_G
Cuts at: 0 1297 1812 2276 2688
Size: 1297 515 464 412
Fragments arranged by size:
1297 515 464 412
AvrII C'CTAG_G
Cuts at: 0 853 2688
Size: 853 1835
BanI G'GyrC_C
Cuts at: 0 1113 1363 1740 1786 2002 2688
Size: 1113 250 377 46 216 686
Fragments arranged by size:
1113 686 377 250 216 46
BanII G_rGCy'C
Cuts at: 0 559 579 993 1280 1838 1877 2284 2567
Size: 559 20 414 287 558 39 407 283
Cuts at: 2567 2688
Size: 121
Fragments arranged by size:
559 558 414 407 287 283 121 39
20
BbeI G_GCGC'C
Cuts at: 0 1117 2688
Size: 1117 1571
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 959 2688
Size: 539 420 1729
Fragments arranged by size:
1729 539 420
BglI GCCn_nnn'nGGC
Cuts at: 0 1795 2688
Size: 1795 893
Bme1580I G_kGCm'C
Cuts at: 0 579 2266 2284 2688
Size: 579 1687 18 404
Fragments arranged by size:
1687 579 404 18
BmgBI CAC'GTC
Cuts at: 0 417 794 2688
Size: 417 377 1894
Fragments arranged by size:
1894 417 377
BmrI ACTGGGnnnn_n'
Cuts at: 0 899 1317 1569 1986 2256 2688
Size: 899 418 252 417 270 432
Fragments arranged by size:
899 432 418 417 270 252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1134 1392 1824 2649 2688
Size: 1134 258 432 825 39
Fragments arranged by size:
1134 825 432 258 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 2688
Size: 760 1928
Bpu10I CC'TnA_GC
Cuts at: 0 1104 1585 1879 2688
Size: 1104 481 294 809
Fragments arranged by size:
1104 809 481 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 2688
Size: 1069 1619
BsaI GGTCTCn'nnnn_
Cuts at: 0 688 2645 2688
Size: 688 1957 43
Fragments arranged by size:
1957 688 43
BsaHI Gr'CG_yC
Cuts at: 0 1114 2688
Size: 1114 1574
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 778 808 1934 1964 2093 2123 2563 2593
Size: 778 30 1126 30 129 30 440 30
Cuts at: 2593 2688
Size: 95
Fragments arranged by size:
1126 778 440 129 95 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 1034 1800 1914 2688
Size: 283 751 766 114 774
Fragments arranged by size:
774 766 751 283 114
BseYI C'CCAG_C
Cuts at: 0 22 162 2118 2299 2688
Size: 22 140 1956 181 389
Fragments arranged by size:
1956 389 181 140 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 2036 2227 2688
Size: 236 1800 191 461
Fragments arranged by size:
1800 461 236 191
BsiEI CG_ry'CG
Cuts at: 0 1784 1799 2688
Size: 1784 15 889
Fragments arranged by size:
1784 889 15
BsiHKAI G_wGCw'C
Cuts at: 0 559 1838 2567 2688
Size: 559 1279 729 121
Fragments arranged by size:
1279 729 559 121
Bsp1286I G_dGCh'C
Cuts at: 0 559 579 993 1280 1838 1877 2266 2284
Size: 559 20 414 287 558 39 389 18
Cuts at: 2284 2567 2688
Size: 283 121
Fragments arranged by size:
559 558 414 389 287 283 121 39
20 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 759 1766 2249 2688
Size: 18 741 1007 483 439
Fragments arranged by size:
1007 741 483 439 18
BsrFI r'CCGG_y
Cuts at: 0 1131 1779 1783 1794 2688
Size: 1131 648 4 11 894
Fragments arranged by size:
1131 894 648 11 4
BssSI C'ACGA_G
Cuts at: 0 587 2204 2566 2688
Size: 587 1617 362 122
Fragments arranged by size:
1617 587 362 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 523 1370 2688
Size: 523 847 1318
Fragments arranged by size:
1318 847 523
BstXI CCAn_nnnn'nTGG
Cuts at: 0 993 1854 2217 2688
Size: 993 861 363 471
Fragments arranged by size:
993 861 471 363
BstYI r'GATC_y
Cuts at: 0 175 222 592 2688
Size: 175 47 370 2096
Fragments arranged by size:
2096 370 175 47
Bsu36I CC'TnA_GG
Cuts at: 0 1616 2688
Size: 1616 1072
BtgI C'CryG_G
Cuts at: 0 947 1337 1388 1884 2232 2688
Size: 947 390 51 496 348 456
Fragments arranged by size:
947 496 456 390 348 51
BtsI GCAGTG_nn'
Cuts at: 0 219 224 1297 2688
Size: 219 5 1073 1391
Fragments arranged by size:
1391 1073 219 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1004 1039 2688
Size: 1004 35 1649
Fragments arranged by size:
1649 1004 35
DraI TTT'AAA
Cuts at: 0 345 2688
Size: 345 2343
DraIII CAC_nnn'GTG
Cuts at: 0 1370 2688
Size: 1370 1318
EaeI y'GGCC_r
Cuts at: 0 1781 1796 2219 2688
Size: 1781 15 423 469
Fragments arranged by size:
1781 469 423 15
EagI C'GGCC_G
Cuts at: 0 1781 1796 2688
Size: 1781 15 892
Fragments arranged by size:
1781 892 15
EarI CTCTTCn'nnn_
Cuts at: 0 500 646 1600 2688
Size: 500 146 954 1088
Fragments arranged by size:
1088 954 500 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 815 1906 2304 2688
Size: 815 1091 398 384
Fragments arranged by size:
1091 815 398 384
EcoICRI GAG'CTC
Cuts at: 0 557 1836 2565 2688
Size: 557 1279 729 123
Fragments arranged by size:
1279 729 557 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 350 760 1259 1966 2684 2688
Size: 296 54 410 499 707 718 4
Fragments arranged by size:
718 707 499 410 296 54 4
EcoO109I rG'GnC_Cy
Cuts at: 0 575 1195 1425 2280 2281 2688
Size: 575 620 230 855 1 407
Fragments arranged by size:
855 620 575 407 230 1
EcoRI G'AATT_C
Cuts at: 0 2604 2688
Size: 2604 84
EcoRV GAT'ATC
Cuts at: 0 2379 2688
Size: 2379 309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2427 2688
Size: 2427 261
FseI GG_CCGG'CC
Cuts at: 0 1798 2688
Size: 1798 890
HaeII r_GCGC'y
Cuts at: 0 1117 2688
Size: 1117 1571
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 687 702 719 734 1003 1035 1138 1170
Size: 687 15 17 15 269 32 103 32
Cuts at: 1170 1297 1329 1530 1562 1717 1747 1749 1779
Size: 127 32 201 32 155 30 2 30
Cuts at: 1779 1810 1842 2093 2125 2642 2668 2674 2688
Size: 31 32 251 32 517 26 6 14
Fragments arranged by size:
687 517 269 251 201 155 127 103
32 32 32 32 32 32 31 30
30 26 17 15 15 14 6 2
HindIII A'AGCT_T
Cuts at: 0 254 292 2688
Size: 254 38 2396
Fragments arranged by size:
2396 254 38
KasI G'GCGC_C
Cuts at: 0 1113 2688
Size: 1113 1575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2069 2445 2688
Size: 2069 376 243
Fragments arranged by size:
2069 376 243
MslI CAynn'nnrTG
Cuts at: 0 1852 2215 2688
Size: 1852 363 473
Fragments arranged by size:
1852 473 363
MspA1I CmG'CkG
Cuts at: 0 18 1351 1710 2118 2688
Size: 18 1333 359 408 570
Fragments arranged by size:
1333 570 408 359 18
NaeI GCC'GGC
Cuts at: 0 1781 1796 2688
Size: 1781 15 892
Fragments arranged by size:
1781 892 15
NarI GG'CG_CC
Cuts at: 0 1114 2688
Size: 1114 1574
NcoI C'CATG_G
Cuts at: 0 947 1388 2688
Size: 947 441 1300
Fragments arranged by size:
1300 947 441
NgoMIV G'CCGG_C
Cuts at: 0 1779 1794 2688
Size: 1779 15 894
Fragments arranged by size:
1779 894 15
NspI r_CATG'y
Cuts at: 0 542 2578 2688
Size: 542 2036 110
Fragments arranged by size:
2036 542 110
PasI CC'CwG_GG
Cuts at: 0 993 2688
Size: 993 1695
PciI A'CATG_T
Cuts at: 0 538 2688
Size: 538 2150
PflMI CCAn_nnn'nTGG
Cuts at: 0 1292 2688
Size: 1292 1396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2563 2595 2688
Size: 2563 32 93
Fragments arranged by size:
2563 93 32
PpuMI rG'GwC_Cy
Cuts at: 0 1195 2688
Size: 1195 1493
PspOMI G'GGCC_C
Cuts at: 0 575 2280 2688
Size: 575 1705 408
Fragments arranged by size:
1705 575 408
PspXI vC'TCGA_Gb
Cuts at: 0 1812 2688
Size: 1812 876
PstI C_TGCA'G
Cuts at: 0 531 1356 2688
Size: 531 825 1332
Fragments arranged by size:
1332 825 531
PvuII CAG'CTG
Cuts at: 0 18 1351 1710 2118 2688
Size: 18 1333 359 408 570
Fragments arranged by size:
1333 570 408 359 18
SacI G_AGCT'C
Cuts at: 0 559 1838 2567 2688
Size: 559 1279 729 121
Fragments arranged by size:
1279 729 559 121
SapI GCTCTTCn'nnn_
Cuts at: 0 500 646 1600 2688
Size: 500 146 954 1088
Fragments arranged by size:
1088 954 500 146
ScaI AGT'ACT
Cuts at: 0 1056 2688
Size: 1056 1632
SfcI C'TryA_G
Cuts at: 0 527 1352 2478 2688
Size: 527 825 1126 210
Fragments arranged by size:
1126 825 527 210
SfoI GGC'GCC
Cuts at: 0 1115 2688
Size: 1115 1573
SmaI CCC'GGG
Cuts at: 0 2278 2688
Size: 2278 410
SmlI C'TyrA_G
Cuts at: 0 1048 1812 2688
Size: 1048 764 876
Fragments arranged by size:
1048 876 764
SspI AAT'ATT
Cuts at: 0 2486 2688
Size: 2486 202
StyI C'CwwG_G
Cuts at: 0 818 853 947 1388 1721 1772 2501 2688
Size: 818 35 94 441 333 51 729 187
Fragments arranged by size:
818 729 441 333 187 94 51 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 74 229 1626 2688
Size: 74 155 1397 1062
Fragments arranged by size:
1397 1062 155 74
TatI w'GTAC_w
Cuts at: 0 1054 2192 2688
Size: 1054 1138 496
Fragments arranged by size:
1138 1054 496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 778 810 2688
Size: 778 32 1878
Fragments arranged by size:
1878 778 32
Tth111I GACn'n_nGTC
Cuts at: 0 958 2688
Size: 958 1730
XbaI T'CTAG_A
Cuts at: 0 1230 2688
Size: 1230 1458
XhoI C'TCGA_G
Cuts at: 0 1812 2688
Size: 1812 876
XmaI C'CCGG_G
Cuts at: 0 2276 2688
Size: 2276 412
XmnI GAAnn'nnTTC
Cuts at: 0 293 2688
Size: 293 2395
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApoI AseI AvaI AvrII BanI BanII BbeI
BbsI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI CspCI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FseI HaeII Hin4I HindIII KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PpiI PpuMI PspOMI PspXI PstI PvuII SacI SapI
ScaI SfcI SfoI SmaI SmlI SspI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI ApaLI
AscI AsiSI BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmtI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I BtgZI ClaI DrdI EcoNI FspI
FspAI HincII HpaI KpnI MfeI MluI MscI NdeI
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI SwaI XcmI ZraI