Restriction map for
pF1KSDA0251
(Linear) (Six-base) MAPSORT of: KIAA0251.seq Check: 2049 from: 1 to: 2364
KIAA0251.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 2033 2364
Size: 2033 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 1327 1666 2364
Size: 1229 98 339 698
Fragments arranged by size:
1229 698 339 98
AflII C'TTAA_G
Cuts at: 0 2358 2364
Size: 2358 6
AflIII A'CryG_T
Cuts at: 0 5 1363 2364
Size: 5 1358 1001
Fragments arranged by size:
1358 1001 5
AleI CACnn'nnGTG
Cuts at: 0 1271 2364
Size: 1271 1093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1384 2364
Size: 1384 980
ApoI r'AATT_y
Cuts at: 0 380 2364
Size: 380 1984
AvaI C'yCGr_G
Cuts at: 0 1409 1787 1806 2076 2230 2364
Size: 1409 378 19 270 154 134
Fragments arranged by size:
1409 378 270 154 134 19
BamHI G'GATC_C
Cuts at: 0 631 2364
Size: 631 1733
BanI G'GyrC_C
Cuts at: 0 1317 2364
Size: 1317 1047
BanII G_rGCy'C
Cuts at: 0 230 238 1154 1763 1936 2265 2364
Size: 230 8 916 609 173 329 99
Fragments arranged by size:
916 609 329 230 173 99 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 482 702 2186 2364
Size: 482 220 1484 178
Fragments arranged by size:
1484 482 220 178
BbvCI CC'TCA_GC
Cuts at: 0 1355 2273 2364
Size: 1355 918 91
Fragments arranged by size:
1355 918 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 937 971 2364
Size: 937 34 1393
Fragments arranged by size:
1393 937 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 341 2364
Size: 341 2023
BfrBI ATG'CAT
Cuts at: 0 207 2364
Size: 207 2157
BlpI GC'TnA_GC
Cuts at: 0 1153 1757 2364
Size: 1153 604 607
Fragments arranged by size:
1153 607 604
Bme1580I G_kGCm'C
Cuts at: 0 1014 1232 1363 2364
Size: 1014 218 131 1001
Fragments arranged by size:
1014 1001 218 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 1156 1219 2364
Size: 1156 63 1145
Fragments arranged by size:
1156 1145 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1173 1284 1821 2178 2364
Size: 1173 111 537 357 186
Fragments arranged by size:
1173 537 357 186 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 100 136 169 682 1462 2029 2364
Size: 34 66 36 33 513 780 567 335
Fragments arranged by size:
780 567 513 335 66 36 34 33
Bpu10I CC'TnA_GC
Cuts at: 0 1355 2273 2364
Size: 1355 918 91
Fragments arranged by size:
1355 918 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1844 2364
Size: 1844 520
BsaI GGTCTCn'nnnn_
Cuts at: 0 1528 1762 2149 2364
Size: 1528 234 387 215
Fragments arranged by size:
1528 387 234 215
BsaAI yAC'GTr
Cuts at: 0 1364 2364
Size: 1364 1000
BsaBI GATnn'nnATC
Cuts at: 0 1424 2364
Size: 1424 940
BsaWI w'CCGG_w
Cuts at: 0 130 1206 1956 2349 2364
Size: 130 1076 750 393 15
Fragments arranged by size:
1076 750 393 130 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 876 906 1406 1436 2049 2079 2364
Size: 876 30 500 30 613 30 285
Fragments arranged by size:
876 613 500 285 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 837 1813 2364
Size: 238 599 976 551
Fragments arranged by size:
976 599 551 238
BseYI C'CCAG_C
Cuts at: 0 635 906 1293 2266 2364
Size: 635 271 387 973 98
Fragments arranged by size:
973 635 387 271 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2069 2364
Size: 2069 295
BsiEI CG_ry'CG
Cuts at: 0 1257 2364
Size: 1257 1107
BsiHKAI G_wGCw'C
Cuts at: 0 1154 1763 2364
Size: 1154 609 601
Fragments arranged by size:
1154 609 601
BsmI GAATG_Cn'
Cuts at: 0 1847 2364
Size: 1847 517
Bsp1286I G_dGCh'C
Cuts at: 0 230 238 1014 1154 1232 1363 1763 1936
Size: 230 8 776 140 78 131 400 173
Cuts at: 1936 2265 2364
Size: 329 99
Fragments arranged by size:
776 400 329 230 173 140 131 99
78 8
BspEI T'CCGG_A
Cuts at: 0 130 2364
Size: 130 2234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1510 2364
Size: 1510 854
BsrDI GCAATG_nn'
Cuts at: 0 1772 2364
Size: 1772 592
BssSI C'ACGA_G
Cuts at: 0 1269 1762 2364
Size: 1269 493 602
Fragments arranged by size:
1269 602 493
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1317 2047 2364
Size: 1317 730 317
Fragments arranged by size:
1317 730 317
BstXI CCAn_nnnn'nTGG
Cuts at: 0 160 253 2364
Size: 160 93 2111
Fragments arranged by size:
2111 160 93
BstYI r'GATC_y
Cuts at: 0 631 1203 1344 1611 1919 2364
Size: 631 572 141 267 308 445
Fragments arranged by size:
631 572 445 308 267 141
BtgI C'CryG_G
Cuts at: 0 901 2364
Size: 901 1463
BtsI GCAGTG_nn'
Cuts at: 0 74 165 2364
Size: 74 91 2199
Fragments arranged by size:
2199 91 74
DraI TTT'AAA
Cuts at: 0 1215 1683 2121 2364
Size: 1215 468 438 243
Fragments arranged by size:
1215 468 438 243
DraIII CAC_nnn'GTG
Cuts at: 0 2047 2364
Size: 2047 317
EaeI y'GGCC_r
Cuts at: 0 1254 1778 2364
Size: 1254 524 586
Fragments arranged by size:
1254 586 524
EagI C'GGCC_G
Cuts at: 0 1254 2364
Size: 1254 1110
EarI CTCTTCn'nnn_
Cuts at: 0 98 413 1484 1705 1895 2364
Size: 98 315 1071 221 190 469
Fragments arranged by size:
1071 469 315 221 190 98
EcoICRI GAG'CTC
Cuts at: 0 1152 1761 2364
Size: 1152 609 603
Fragments arranged by size:
1152 609 603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 100 136 169 682 1229 1327 1462
Size: 34 66 36 33 513 547 98 135
Cuts at: 1462 1666 2029 2364
Size: 204 363 335
Fragments arranged by size:
547 513 363 335 204 135 98 66
36 34 33
EcoO109I rG'GnC_Cy
Cuts at: 0 1690 2126 2310 2364
Size: 1690 436 184 54
Fragments arranged by size:
1690 436 184 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1909 2364
Size: 1909 455
HaeII r_GCGC'y
Cuts at: 0 2202 2364
Size: 2202 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 876 908 2188 2220 2317 2349 2364
Size: 876 32 1280 32 97 32 15
Fragments arranged by size:
1280 876 97 32 32 32 15
HincII GTy'rAC
Cuts at: 0 1084 1093 2364
Size: 1084 9 1271
Fragments arranged by size:
1271 1084 9
MluI A'CGCG_T
Cuts at: 0 5 2364
Size: 5 2359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2092 2364
Size: 2092 272
MslI CAynn'nnrTG
Cuts at: 0 212 886 1271 1339 1583 2364
Size: 212 674 385 68 244 781
Fragments arranged by size:
781 674 385 244 212 68
MspA1I CmG'CkG
Cuts at: 0 919 1305 1314 2364
Size: 919 386 9 1050
Fragments arranged by size:
1050 919 386 9
NdeI CA'TA_TG
Cuts at: 0 269 793 2364
Size: 269 524 1571
Fragments arranged by size:
1571 524 269
NsiI A_TGCA'T
Cuts at: 0 209 2364
Size: 209 2155
NspI r_CATG'y
Cuts at: 0 1078 1582 2364
Size: 1078 504 782
Fragments arranged by size:
1078 782 504
PasI CC'CwG_GG
Cuts at: 0 2257 2364
Size: 2257 107
PflMI CCAn_nnn'nTGG
Cuts at: 0 368 2364
Size: 368 1996
PfoI T'CCnGG_A
Cuts at: 0 659 1184 2364
Size: 659 525 1180
Fragments arranged by size:
1180 659 525
PmlI CAC'GTG
Cuts at: 0 1364 2364
Size: 1364 1000
PpuMI rG'GwC_Cy
Cuts at: 0 2126 2310 2364
Size: 2126 184 54
Fragments arranged by size:
2126 184 54
PspXI vC'TCGA_Gb
Cuts at: 0 1806 2230 2364
Size: 1806 424 134
Fragments arranged by size:
1806 424 134
PstI C_TGCA'G
Cuts at: 0 867 1874 2364
Size: 867 1007 490
Fragments arranged by size:
1007 867 490
PvuII CAG'CTG
Cuts at: 0 919 1305 1314 2364
Size: 919 386 9 1050
Fragments arranged by size:
1050 919 386 9
SacI G_AGCT'C
Cuts at: 0 1154 1763 2364
Size: 1154 609 601
Fragments arranged by size:
1154 609 601
SanDI GG'GwC_CC
Cuts at: 0 2310 2364
Size: 2310 54
SapI GCTCTTCn'nnn_
Cuts at: 0 1705 2364
Size: 1705 659
SfcI C'TryA_G
Cuts at: 0 863 1870 1925 2364
Size: 863 1007 55 439
Fragments arranged by size:
1007 863 439 55
SmaI CCC'GGG
Cuts at: 0 1789 2364
Size: 1789 575
SmlI C'TyrA_G
Cuts at: 0 1806 1859 2230 2358 2364
Size: 1806 53 371 128 6
Fragments arranged by size:
1806 371 128 53 6
SphI G_CATG'C
Cuts at: 0 1078 2364
Size: 1078 1286
SspI AAT'ATT
Cuts at: 0 252 2364
Size: 252 2112
StuI AGG'CCT
Cuts at: 0 2296 2364
Size: 2296 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 389 2270 2305 2333 2364
Size: 389 1881 35 28 31
Fragments arranged by size:
1881 389 35 31 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1406 1438 2049 2081 2364
Size: 1406 32 611 32 283
Fragments arranged by size:
1406 611 283 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 287 1744 2364
Size: 287 1457 620
Fragments arranged by size:
1457 620 287
XhoI C'TCGA_G
Cuts at: 0 1806 2230 2364
Size: 1806 424 134
Fragments arranged by size:
1806 424 134
XmaI C'CCGG_G
Cuts at: 0 1787 2364
Size: 1787 577
XmnI GAAnn'nnTTC
Cuts at: 0 1835 1892 2364
Size: 1835 57 472
Fragments arranged by size:
1835 472 57
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BfrBI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BssSI BstAPI
BstXI BstYI BtgI BtsI DraI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
MluI MmeI MslI MspA1I NdeI NsiI NspI PasI
PflMI PfoI PmlI PpuMI PspXI PstI PvuII SacI
SanDI SapI SfcI SmaI SmlI SphI SspI StuI
TaqII TstI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BclI BglI BglII BmgBI BmtI BsaHI BsiWI
BsmBI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
StyI SwaI TatI XbaI XcmI ZraI