Restriction map for
pF1KSDA0252
(Linear) (Six-base) MAPSORT of: KIAA0252.seq Check: 5637 from: 1 to: 2010
KIAA0252.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AatII G_ACGT'C
Cuts at: 0 172 2010
Size: 172 1838
AccI GT'mk_AC
Cuts at: 0 812 2010
Size: 812 1198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1450 2010
Size: 968 482 560
Fragments arranged by size:
968 560 482
AflIII A'CryG_T
Cuts at: 0 1182 2010
Size: 1182 828
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1291 2010
Size: 1291 719
AlwNI CAG_nnn'CTG
Cuts at: 0 328 352 1428 2010
Size: 328 24 1076 582
Fragments arranged by size:
1076 582 328 24
ApaI G_GGCC'C
Cuts at: 0 462 2010
Size: 462 1548
ApoI r'AATT_y
Cuts at: 0 1216 1336 1818 2010
Size: 1216 120 482 192
Fragments arranged by size:
1216 482 192 120
BamHI G'GATC_C
Cuts at: 0 1653 2010
Size: 1653 357
BanI G'GyrC_C
Cuts at: 0 1135 2010
Size: 1135 875
BanII G_rGCy'C
Cuts at: 0 83 322 462 776 1959 2010
Size: 83 239 140 314 1183 51
Fragments arranged by size:
1183 314 239 140 83 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 458 1423 1988 2010
Size: 437 21 965 565 22
Fragments arranged by size:
965 565 437 22 21
BbvCI CC'TCA_GC
Cuts at: 0 1448 2010
Size: 1448 562
BclI T'GATC_A
Cuts at: 0 1344 2010
Size: 1344 666
BglI GCCn_nnn'nGGC
Cuts at: 0 1618 1729 2010
Size: 1618 111 281
Fragments arranged by size:
1618 281 111
BglII A'GATC_T
Cuts at: 0 1848 1966 2010
Size: 1848 118 44
Fragments arranged by size:
1848 118 44
BlpI GC'TnA_GC
Cuts at: 0 321 770 2010
Size: 321 449 1240
Fragments arranged by size:
1240 449 321
Bme1580I G_kGCm'C
Cuts at: 0 462 2010
Size: 462 1548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 831 1221 2010
Size: 451 380 390 789
Fragments arranged by size:
789 451 390 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1525 1778 2010
Size: 1525 253 232
Fragments arranged by size:
1525 253 232
Bpu10I CC'TnA_GC
Cuts at: 0 1448 2010
Size: 1448 562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 2010
Size: 701 1309
BsaI GGTCTCn'nnnn_
Cuts at: 0 1697 2010
Size: 1697 313
BsaBI GATnn'nnATC
Cuts at: 0 1048 2010
Size: 1048 962
BsaHI Gr'CG_yC
Cuts at: 0 169 2010
Size: 169 1841
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 2010
Size: 70 30 1910
Fragments arranged by size:
1910 70 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 273 357 628 742 844 916 2010
Size: 133 140 84 271 114 102 72 1094
Fragments arranged by size:
1094 271 140 133 114 102 84 72
BseYI C'CCAG_C
Cuts at: 0 1707 1733 1897 2010
Size: 1707 26 164 113
Fragments arranged by size:
1707 164 113 26
BsiHKAI G_wGCw'C
Cuts at: 0 83 322 776 2010
Size: 83 239 454 1234
Fragments arranged by size:
1234 454 239 83
BsmI GAATG_Cn'
Cuts at: 0 1746 2010
Size: 1746 264
BsmBI CGTCTCn'nnnn_
Cuts at: 0 160 2010
Size: 160 1850
Bsp1286I G_dGCh'C
Cuts at: 0 83 322 462 776 1959 2010
Size: 83 239 140 314 1183 51
Fragments arranged by size:
1183 314 239 140 83 51
BsrBI CCG'CTC
Cuts at: 0 869 2010
Size: 869 1141
BsrDI GCAATG_nn'
Cuts at: 0 1179 2010
Size: 1179 831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1132 2010
Size: 1132 878
BstYI r'GATC_y
Cuts at: 0 914 1653 1848 1966 2010
Size: 914 739 195 118 44
Fragments arranged by size:
914 739 195 118 44
Bsu36I CC'TnA_GG
Cuts at: 0 285 329 2010
Size: 285 44 1681
Fragments arranged by size:
1681 285 44
BtgI C'CryG_G
Cuts at: 0 722 1442 2010
Size: 722 720 568
Fragments arranged by size:
722 720 568
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1267 2010
Size: 1267 743
BtsI GCAGTG_nn'
Cuts at: 0 114 1678 2010
Size: 114 1564 332
Fragments arranged by size:
1564 332 114
EarI CTCTTCn'nnn_
Cuts at: 0 375 396 401 514 605 821 830 893
Size: 375 21 5 113 91 216 9 63
Cuts at: 893 905 1352 2010
Size: 12 447 658
Fragments arranged by size:
658 447 375 216 113 91 63 21
12 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1928 2010
Size: 1928 82
EcoICRI GAG'CTC
Cuts at: 0 81 320 774 2010
Size: 81 239 454 1236
Fragments arranged by size:
1236 454 239 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1450 1525 1778 2010
Size: 968 482 75 253 232
Fragments arranged by size:
968 482 253 232 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 73 332 2010
Size: 73 259 1678
Fragments arranged by size:
1678 259 73
EcoO109I rG'GnC_Cy
Cuts at: 0 458 1452 1521 1611 2010
Size: 458 994 69 90 399
Fragments arranged by size:
994 458 399 90 69
EcoRI G'AATT_C
Cuts at: 0 1216 1818 2010
Size: 1216 602 192
Fragments arranged by size:
1216 602 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1342 1902 2010
Size: 1342 560 108
Fragments arranged by size:
1342 560 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 373 379 405 411 533 565
Size: 70 32 271 6 26 6 122 32
Cuts at: 565 697 729 832 856 864 888 1779 1811
Size: 132 32 103 24 8 24 891 32
Cuts at: 1811 2010
Size: 199
Fragments arranged by size:
891 271 199 132 122 103 70 32
32 32 32 26 24 24 8 6
6
MluI A'CGCG_T
Cuts at: 0 1182 2010
Size: 1182 828
MslI CAynn'nnrTG
Cuts at: 0 1172 2010
Size: 1172 838
MspA1I CmG'CkG
Cuts at: 0 53 313 769 1536 1575 1711 1737 2010
Size: 53 260 456 767 39 136 26 273
Fragments arranged by size:
767 456 273 260 136 53 39 26
NcoI C'CATG_G
Cuts at: 0 722 1442 2010
Size: 722 720 568
Fragments arranged by size:
722 720 568
NspI r_CATG'y
Cuts at: 0 1005 1272 2010
Size: 1005 267 738
Fragments arranged by size:
1005 738 267
PflMI CCAn_nnn'nTGG
Cuts at: 0 1650 2010
Size: 1650 360
PpuMI rG'GwC_Cy
Cuts at: 0 1452 2010
Size: 1452 558
PsiI TTA'TAA
Cuts at: 0 1334 2010
Size: 1334 676
PspOMI G'GGCC_C
Cuts at: 0 458 2010
Size: 458 1552
PstI C_TGCA'G
Cuts at: 0 150 2010
Size: 150 1860
PvuII CAG'CTG
Cuts at: 0 313 769 1711 1737 2010
Size: 313 456 942 26 273
Fragments arranged by size:
942 456 313 273 26
SacI G_AGCT'C
Cuts at: 0 83 322 776 2010
Size: 83 239 454 1234
Fragments arranged by size:
1234 454 239 83
SfcI C'TryA_G
Cuts at: 0 146 2010
Size: 146 1864
SmlI C'TyrA_G
Cuts at: 0 716 2010
Size: 716 1294
SphI G_CATG'C
Cuts at: 0 1272 2010
Size: 1272 738
StyI C'CwwG_G
Cuts at: 0 722 1442 1931 1946 1960 2010
Size: 722 720 489 15 14 50
Fragments arranged by size:
722 720 489 50 15 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1413 2010
Size: 1413 597
TatI w'GTAC_w
Cuts at: 0 1860 2010
Size: 1860 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1022 1054 2010
Size: 1022 32 956
Fragments arranged by size:
1022 956 32
XbaI T'CTAG_A
Cuts at: 0 1284 2010
Size: 1284 726
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 2010
Size: 421 1589
ZraI GAC'GTC
Cuts at: 0 170 2010
Size: 170 1840
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI AlwNI ApaI ApoI
BamHI BanI BanII BbsI BbvCI BclI BglI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BsrBI BsrDI BstXI BstYI Bsu36I BtgI BtgZI BtsI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I MluI MslI MspA1I NcoI NspI PflMI PpuMI
PsiI PspOMI PstI PvuII SacI SfcI SmlI SphI
StyI TaqII TatI TstI XbaI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BfrBI BmgBI BmrI BmtI BsaAI
BsaWI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XhoI XmaI XmnI