Restriction map for
pF1KA0255
(Linear) (Six-base) MAPSORT of: KIAA0255.seq Check: 1349 from: 1 to: 1875
KIAA0255.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AatII G_ACGT'C
Cuts at: 0 776 950 1875
Size: 776 174 925
Fragments arranged by size:
925 776 174
AccI GT'mk_AC
Cuts at: 0 1340 1875
Size: 1340 535
Acc65I G'GTAC_C
Cuts at: 0 643 1810 1875
Size: 643 1167 65
Fragments arranged by size:
1167 643 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1497 1875
Size: 936 561 378
Fragments arranged by size:
936 561 378
AflIII A'CryG_T
Cuts at: 0 548 1835 1875
Size: 548 1287 40
Fragments arranged by size:
1287 548 40
AleI CACnn'nnGTG
Cuts at: 0 1294 1875
Size: 1294 581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1519 1551 1875
Size: 1519 32 324
Fragments arranged by size:
1519 324 32
ApaLI G'TGCA_C
Cuts at: 0 627 937 1875
Size: 627 310 938
Fragments arranged by size:
938 627 310
ApoI r'AATT_y
Cuts at: 0 1659 1875
Size: 1659 216
AvaI C'yCGr_G
Cuts at: 0 1420 1875
Size: 1420 455
BanI G'GyrC_C
Cuts at: 0 643 1278 1483 1810 1875
Size: 643 635 205 327 65
Fragments arranged by size:
643 635 327 205 65
BanII G_rGCy'C
Cuts at: 0 386 997 1080 1502 1681 1875
Size: 386 611 83 422 179 194
Fragments arranged by size:
611 422 386 194 179 83
BbeI G_GCGC'C
Cuts at: 0 1487 1875
Size: 1487 388
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 810 911 942 1875
Size: 335 475 101 31 933
Fragments arranged by size:
933 475 335 101 31
BbvCI CC'TCA_GC
Cuts at: 0 979 1875
Size: 979 896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1215 1249 1299 1333 1875
Size: 1215 34 50 34 542
Fragments arranged by size:
1215 542 50 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 844 1596 1875
Size: 844 752 279
Fragments arranged by size:
844 752 279
BglII A'GATC_T
Cuts at: 0 476 1850 1875
Size: 476 1374 25
Fragments arranged by size:
1374 476 25
Bme1580I G_kGCm'C
Cuts at: 0 631 941 1283 1875
Size: 631 310 342 592
Fragments arranged by size:
631 592 342 310
BmrI ACTGGGnnnn_n'
Cuts at: 0 723 957 1875
Size: 723 234 918
Fragments arranged by size:
918 723 234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 1875
Size: 405 1470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 616 1050 1875
Size: 400 216 434 825
Fragments arranged by size:
825 434 400 216
Bpu10I CC'TnA_GC
Cuts at: 0 838 979 996 1875
Size: 838 141 17 879
Fragments arranged by size:
879 838 141 17
BsaAI yAC'GTr
Cuts at: 0 551 1875
Size: 551 1324
BsaBI GATnn'nnATC
Cuts at: 0 1024 1875
Size: 1024 851
BsaHI Gr'CG_yC
Cuts at: 0 773 947 1484 1875
Size: 773 174 537 391
Fragments arranged by size:
773 537 391 174
BsaWI w'CCGG_w
Cuts at: 0 197 863 1875
Size: 197 666 1012
Fragments arranged by size:
1012 666 197
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 910 940 1519 1549 1875
Size: 535 30 345 30 579 30 326
Fragments arranged by size:
579 535 345 326 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 642 931 1747 1875
Size: 642 289 816 128
Fragments arranged by size:
816 642 289 128
BseYI C'CCAG_C
Cuts at: 0 104 145 746 990 1465 1875
Size: 104 41 601 244 475 410
Fragments arranged by size:
601 475 410 244 104 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 451 469 1652 1875
Size: 279 172 18 1183 223
Fragments arranged by size:
1183 279 223 172 18
BsiHKAI G_wGCw'C
Cuts at: 0 386 548 631 941 1080 1502 1875
Size: 386 162 83 310 139 422 373
Fragments arranged by size:
422 386 373 310 162 139 83
BsmI GAATG_Cn'
Cuts at: 0 1001 1684 1875
Size: 1001 683 191
Fragments arranged by size:
1001 683 191
Bsp1286I G_dGCh'C
Cuts at: 0 386 548 631 941 997 1080 1283 1502
Size: 386 162 83 310 56 83 203 219
Cuts at: 1502 1681 1875
Size: 179 194
Fragments arranged by size:
386 310 219 203 194 179 162 83
83 56
BspEI T'CCGG_A
Cuts at: 0 863 1875
Size: 863 1012
BspHI T'CATG_A
Cuts at: 0 227 1082 1875
Size: 227 855 793
Fragments arranged by size:
855 793 227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 368 491 1875
Size: 368 123 1384
Fragments arranged by size:
1384 368 123
BsrBI CCG'CTC
Cuts at: 0 318 1276 1875
Size: 318 958 599
Fragments arranged by size:
958 599 318
BsrDI GCAATG_nn'
Cuts at: 0 347 872 1875
Size: 347 525 1003
Fragments arranged by size:
1003 525 347
BsrFI r'CCGG_y
Cuts at: 0 449 1875
Size: 449 1426
BssSI C'ACGA_G
Cuts at: 0 307 1875
Size: 307 1568
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 1296 1875
Size: 378 918 579
Fragments arranged by size:
918 579 378
BstYI r'GATC_y
Cuts at: 0 476 779 1322 1415 1469 1850 1875
Size: 476 303 543 93 54 381 25
Fragments arranged by size:
543 476 381 303 93 54 25
Bsu36I CC'TnA_GG
Cuts at: 0 638 1875
Size: 638 1237
BtgI C'CryG_G
Cuts at: 0 911 1292 1875
Size: 911 381 583
Fragments arranged by size:
911 583 381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 419 1875
Size: 419 1456
BtsI GCAGTG_nn'
Cuts at: 0 127 1875
Size: 127 1748
DraI TTT'AAA
Cuts at: 0 1160 1875
Size: 1160 715
DrdI GACnn_nn'nnGTC
Cuts at: 0 202 337 1875
Size: 202 135 1538
Fragments arranged by size:
1538 202 135
EaeI y'GGCC_r
Cuts at: 0 311 368 1140 1875
Size: 311 57 772 735
Fragments arranged by size:
772 735 311 57
EarI CTCTTCn'nnn_
Cuts at: 0 527 613 1108 1507 1875
Size: 527 86 495 399 368
Fragments arranged by size:
527 495 399 368 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1142 1875
Size: 1142 733
EcoICRI GAG'CTC
Cuts at: 0 384 1078 1500 1875
Size: 384 694 422 375
Fragments arranged by size:
694 422 384 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 616 936 1050 1497 1875
Size: 400 216 320 114 447 378
Fragments arranged by size:
447 400 378 320 216 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 865 1875
Size: 865 1010
EcoO109I rG'GnC_Cy
Cuts at: 0 600 956 1875
Size: 600 356 919
Fragments arranged by size:
919 600 356
EcoRV GAT'ATC
Cuts at: 0 729 1875
Size: 729 1146
FspI TGC'GCA
Cuts at: 0 1393 1875
Size: 1393 482
FspAI rTGC'GCAy
Cuts at: 0 1393 1875
Size: 1393 482
HaeII r_GCGC'y
Cuts at: 0 17 1487 1875
Size: 17 1470 388
Fragments arranged by size:
1470 388 17
HincII GTy'rAC
Cuts at: 0 207 471 1722 1875
Size: 207 264 1251 153
Fragments arranged by size:
1251 264 207 153
HpaI GTT'AAC
Cuts at: 0 1722 1875
Size: 1722 153
KasI G'GCGC_C
Cuts at: 0 1483 1875
Size: 1483 392
KpnI G_GTAC'C
Cuts at: 0 647 1814 1875
Size: 647 1167 61
Fragments arranged by size:
1167 647 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 416 1875
Size: 293 123 1459
Fragments arranged by size:
1459 293 123
MscI TGG'CCA
Cuts at: 0 370 1875
Size: 370 1505
MslI CAynn'nnrTG
Cuts at: 0 1111 1117 1294 1459 1579 1609 1875
Size: 1111 6 177 165 120 30 266
Fragments arranged by size:
1111 266 177 165 120 30 6
MspA1I CmG'CkG
Cuts at: 0 690 1008 1428 1626 1646 1875
Size: 690 318 420 198 20 229
Fragments arranged by size:
690 420 318 229 198 20
NarI GG'CG_CC
Cuts at: 0 1484 1875
Size: 1484 391
NcoI C'CATG_G
Cuts at: 0 911 1292 1875
Size: 911 381 583
Fragments arranged by size:
911 583 381
NdeI CA'TA_TG
Cuts at: 0 1377 1875
Size: 1377 498
NspI r_CATG'y
Cuts at: 0 1314 1839 1875
Size: 1314 525 36
Fragments arranged by size:
1314 525 36
PciI A'CATG_T
Cuts at: 0 1835 1875
Size: 1835 40
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1519 1551 1875
Size: 1519 32 324
Fragments arranged by size:
1519 324 32
PpuMI rG'GwC_Cy
Cuts at: 0 600 1875
Size: 600 1275
PvuII CAG'CTG
Cuts at: 0 690 1008 1626 1875
Size: 690 318 618 249
Fragments arranged by size:
690 618 318 249
SacI G_AGCT'C
Cuts at: 0 386 1080 1502 1875
Size: 386 694 422 373
Fragments arranged by size:
694 422 386 373
SapI GCTCTTCn'nnn_
Cuts at: 0 1507 1875
Size: 1507 368
SfoI GGC'GCC
Cuts at: 0 1485 1875
Size: 1485 390
StyI C'CwwG_G
Cuts at: 0 677 911 1292 1875
Size: 677 234 381 583
Fragments arranged by size:
677 583 381 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 914 1875
Size: 914 961
TatI w'GTAC_w
Cuts at: 0 692 1616 1875
Size: 692 924 259
Fragments arranged by size:
924 692 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 910 942 1875
Size: 910 32 933
Fragments arranged by size:
933 910 32
XmnI GAAnn'nnTTC
Cuts at: 0 1246 1534 1875
Size: 1246 288 341
Fragments arranged by size:
1246 341 288
ZraI GAC'GTC
Cuts at: 0 774 948 1875
Size: 774 174 927
Fragments arranged by size:
927 774 174
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AloI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FspI FspAI HaeII HincII HpaI KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NdeI NspI
PciI PpiI PpuMI PvuII SacI SapI SfoI StyI
TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AlfI AlwNI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BclI
BfrBI BglI BlpI BmgBI BmtI BpuEI BsaI BsiEI
BsiWI BsmBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
ClaI CspCI DraIII EagI EcoRI FalI FseI Hin4I
HindIII MfeI MluI NaeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI