Restriction map for
pF1KA0256
(Linear) (Six-base) MAPSORT of: KIAA0256.seq Check: 6319 from: 1 to: 3168
KIAA0256.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 907 3168
Size: 907 2261
AccI GT'mk_AC
Cuts at: 0 316 3168
Size: 316 2852
Acc65I G'GTAC_C
Cuts at: 0 2382 3168
Size: 2382 786
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2486 2501 2941 3168
Size: 2486 15 440 227
Fragments arranged by size:
2486 440 227 15
AflIII A'CryG_T
Cuts at: 0 1346 3168
Size: 1346 1822
AlwNI CAG_nnn'CTG
Cuts at: 0 613 2746 3113 3168
Size: 613 2133 367 55
Fragments arranged by size:
2133 613 367 55
ApoI r'AATT_y
Cuts at: 0 2165 2781 3168
Size: 2165 616 387
Fragments arranged by size:
2165 616 387
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2798 2831 3168
Size: 2798 33 337
Fragments arranged by size:
2798 337 33
BamHI G'GATC_C
Cuts at: 0 1657 3168
Size: 1657 1511
BanI G'GyrC_C
Cuts at: 0 2382 3027 3168
Size: 2382 645 141
Fragments arranged by size:
2382 645 141
BanII G_rGCy'C
Cuts at: 0 12 3168
Size: 12 3156
BbeI G_GCGC'C
Cuts at: 0 3031 3168
Size: 3031 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 462 2996 3168
Size: 462 2534 172
Fragments arranged by size:
2534 462 172
BbvCI CC'TCA_GC
Cuts at: 0 41 2354 2655 3168
Size: 41 2313 301 513
Fragments arranged by size:
2313 513 301 41
BciVI GTATCCnnnnn_n'
Cuts at: 0 2565 3168
Size: 2565 603
BglII A'GATC_T
Cuts at: 0 2772 3168
Size: 2772 396
BlpI GC'TnA_GC
Cuts at: 0 293 3168
Size: 293 2875
Bme1580I G_kGCm'C
Cuts at: 0 351 3168
Size: 351 2817
BmrI ACTGGGnnnn_n'
Cuts at: 0 1742 2579 3168
Size: 1742 837 589
Fragments arranged by size:
1742 837 589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2766 3168
Size: 2766 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1751 3061 3168
Size: 1751 1310 107
Fragments arranged by size:
1751 1310 107
Bpu10I CC'TnA_GC
Cuts at: 0 41 1076 2354 2655 3168
Size: 41 1035 1278 301 513
Fragments arranged by size:
1278 1035 513 301 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 3168
Size: 1226 1942
BsaI GGTCTCn'nnnn_
Cuts at: 0 694 2926 2931 3168
Size: 694 2232 5 237
Fragments arranged by size:
2232 694 237 5
BsaHI Gr'CG_yC
Cuts at: 0 3028 3168
Size: 3028 140
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2314 2866 2869 2872 3168
Size: 2314 552 3 3 296
Fragments arranged by size:
2314 552 296 3 3
BseYI C'CCAG_C
Cuts at: 0 151 2668 3168
Size: 151 2517 500
Fragments arranged by size:
2517 500 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 935 1348 2917 3168
Size: 202 733 413 1569 251
Fragments arranged by size:
1569 733 413 251 202
BsiHKAI G_wGCw'C
Cuts at: 0 3137 3168
Size: 3137 31
BsmI GAATG_Cn'
Cuts at: 0 442 3168
Size: 442 2726
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2004 3168
Size: 2004 1164
Bsp1286I G_dGCh'C
Cuts at: 0 12 351 3137 3168
Size: 12 339 2786 31
Fragments arranged by size:
2786 339 31 12
BspHI T'CATG_A
Cuts at: 0 2874 3168
Size: 2874 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 598 907 3168
Size: 598 309 2261
Fragments arranged by size:
2261 598 309
BsrDI GCAATG_nn'
Cuts at: 0 1385 1520 2342 3168
Size: 1385 135 822 826
Fragments arranged by size:
1385 826 822 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 346 1231 2425 3070 3168
Size: 346 885 1194 645 98
Fragments arranged by size:
1194 885 645 346 98
BstYI r'GATC_y
Cuts at: 0 1657 1986 2772 3168
Size: 1657 329 786 396
Fragments arranged by size:
1657 786 396 329
BstZ17I GTA'TAC
Cuts at: 0 317 3168
Size: 317 2851
Bsu36I CC'TnA_GG
Cuts at: 0 3032 3168
Size: 3032 136
BtgI C'CryG_G
Cuts at: 0 14 2147 3168
Size: 14 2133 1021
Fragments arranged by size:
2133 1021 14
BtsI GCAGTG_nn'
Cuts at: 0 704 2448 3168
Size: 704 1744 720
Fragments arranged by size:
1744 720 704
DraI TTT'AAA
Cuts at: 0 1604 3168
Size: 1604 1564
DraIII CAC_nnn'GTG
Cuts at: 0 346 3168
Size: 346 2822
DrdI GACnn_nn'nnGTC
Cuts at: 0 1803 3168
Size: 1803 1365
EarI CTCTTCn'nnn_
Cuts at: 0 64 738 1943 3008 3168
Size: 64 674 1205 1065 160
Fragments arranged by size:
1205 1065 674 160 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1751 2486 2501 2941 3061 3168
Size: 1751 735 15 440 120 107
Fragments arranged by size:
1751 735 440 120 107 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 854 3168
Size: 854 2314
EcoO109I rG'GnC_Cy
Cuts at: 0 610 3168
Size: 610 2558
EcoRI G'AATT_C
Cuts at: 0 2781 3168
Size: 2781 387
EcoRV GAT'ATC
Cuts at: 0 678 3168
Size: 678 2490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1647 3168
Size: 1647 1521
HaeII r_GCGC'y
Cuts at: 0 1632 3031 3168
Size: 1632 1399 137
Fragments arranged by size:
1632 1399 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 503 535 607 639 655 687 876 908
Size: 503 32 72 32 16 32 189 32
Cuts at: 908 3168
Size: 2260
Fragments arranged by size:
2260 503 189 72 32 32 32 32
16
HindIII A'AGCT_T
Cuts at: 0 1214 1286 3168
Size: 1214 72 1882
Fragments arranged by size:
1882 1214 72
KasI G'GCGC_C
Cuts at: 0 3027 3168
Size: 3027 141
KpnI G_GTAC'C
Cuts at: 0 2386 3168
Size: 2386 782
MfeI C'AATT_G
Cuts at: 0 2612 3168
Size: 2612 556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 3168
Size: 416 2752
MslI CAynn'nnrTG
Cuts at: 0 909 3168
Size: 909 2259
MspA1I CmG'CkG
Cuts at: 0 40 330 567 1303 3168
Size: 40 290 237 736 1865
Fragments arranged by size:
1865 736 290 237 40
NarI GG'CG_CC
Cuts at: 0 3028 3168
Size: 3028 140
NcoI C'CATG_G
Cuts at: 0 2147 3168
Size: 2147 1021
NdeI CA'TA_TG
Cuts at: 0 2048 3168
Size: 2048 1120
NspI r_CATG'y
Cuts at: 0 1350 1746 2826 3168
Size: 1350 396 1080 342
Fragments arranged by size:
1350 1080 396 342
PciI A'CATG_T
Cuts at: 0 1346 3168
Size: 1346 1822
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 3168
Size: 357 2811
PfoI T'CCnGG_A
Cuts at: 0 1964 3168
Size: 1964 1204
PpuMI rG'GwC_Cy
Cuts at: 0 610 3168
Size: 610 2558
PshAI GACnn'nnGTC
Cuts at: 0 1413 3168
Size: 1413 1755
PsiI TTA'TAA
Cuts at: 0 2077 3168
Size: 2077 1091
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1771 1803 3168
Size: 1771 32 1365
Fragments arranged by size:
1771 1365 32
PstI C_TGCA'G
Cuts at: 0 826 855 1437 2421 2443 3118 3168
Size: 826 29 582 984 22 675 50
Fragments arranged by size:
984 826 675 582 50 29 22
PvuII CAG'CTG
Cuts at: 0 40 330 1303 3168
Size: 40 290 973 1865
Fragments arranged by size:
1865 973 290 40
SapI GCTCTTCn'nnn_
Cuts at: 0 738 3168
Size: 738 2430
ScaI AGT'ACT
Cuts at: 0 2649 3168
Size: 2649 519
SfcI C'TryA_G
Cuts at: 0 308 822 851 1433 2417 2439 2639 2888
Size: 308 514 29 582 984 22 200 249
Cuts at: 2888 3114 3168
Size: 226 54
Fragments arranged by size:
984 582 514 308 249 226 200 54
29 22
SfoI GGC'GCC
Cuts at: 0 3029 3168
Size: 3029 139
SmlI C'TyrA_G
Cuts at: 0 1241 3168
Size: 1241 1927
SphI G_CATG'C
Cuts at: 0 1746 2826 3168
Size: 1746 1080 342
Fragments arranged by size:
1746 1080 342
SspI AAT'ATT
Cuts at: 0 1089 3168
Size: 1089 2079
StyI C'CwwG_G
Cuts at: 0 728 967 2147 2184 3168
Size: 728 239 1180 37 984
Fragments arranged by size:
1180 984 728 239 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 20 3168
Size: 20 3148
TatI w'GTAC_w
Cuts at: 0 2647 2737 3168
Size: 2647 90 431
Fragments arranged by size:
2647 431 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 432 464 1167 1199 2195 2227 3042 3074
Size: 432 32 703 32 996 32 815 32
Cuts at: 3074 3168
Size: 94
Fragments arranged by size:
996 815 703 432 94 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 994 3168
Size: 994 2174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 894 1729 2110 3168
Size: 894 835 381 1058
Fragments arranged by size:
1058 894 835 381
XmnI GAAnn'nnTTC
Cuts at: 0 123 1026 2954 3168
Size: 123 903 1928 214
Fragments arranged by size:
1928 903 214 123
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlwNI ApoI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BstAPI BstYI BstZ17I Bsu36I BtgI
BtsI DraI DraIII DrdI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HindIII KasI KpnI
MfeI MmeI MslI MspA1I NarI NcoI NdeI NspI
PciI PflMI PfoI PpuMI PshAI PsiI PsrI PstI
PvuII SapI ScaI SfcI SfoI SmlI SphI SspI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BcgI BclI BfrBI BglI BmgBI BmtI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BtgZI ClaI CspCI
EaeI EagI EciI EcoICRI FseI FspI FspAI HincII
HpaI MluI MscI NaeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XhoI XmaI ZraI