Restriction map for
pF1KSDA0258
(Linear) (Six-base) MAPSORT of: KIAA0258.seq Check: 3751 from: 1 to: 1173
KIAA0258.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 404 1073 1173
Size: 404 669 100
Fragments arranged by size:
669 404 100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1065 1173
Size: 475 590 108
Fragments arranged by size:
590 475 108
AflIII A'CryG_T
Cuts at: 0 846 1173
Size: 846 327
AleI CACnn'nnGTG
Cuts at: 0 251 1173
Size: 251 922
AlwNI CAG_nnn'CTG
Cuts at: 0 1057 1173
Size: 1057 116
ApoI r'AATT_y
Cuts at: 0 290 979 1173
Size: 290 689 194
Fragments arranged by size:
689 290 194
AvaI C'yCGr_G
Cuts at: 0 992 1173
Size: 992 181
BanI G'GyrC_C
Cuts at: 0 863 1173
Size: 863 310
BanII G_rGCy'C
Cuts at: 0 23 1173
Size: 23 1150
BciVI GTATCCnnnnn_n'
Cuts at: 0 280 1173
Size: 280 893
BlpI GC'TnA_GC
Cuts at: 0 22 584 1173
Size: 22 562 589
Fragments arranged by size:
589 562 22
Bme1580I G_kGCm'C
Cuts at: 0 141 1173
Size: 141 1032
BmgBI CAC'GTC
Cuts at: 0 714 817 1173
Size: 714 103 356
Fragments arranged by size:
714 356 103
BmtI G_CTAG'C
Cuts at: 0 583 1173
Size: 583 590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 341 1173
Size: 79 262 832
Fragments arranged by size:
832 262 79
Bpu10I CC'TnA_GC
Cuts at: 0 1058 1173
Size: 1058 115
BsaWI w'CCGG_w
Cuts at: 0 500 1173
Size: 500 673
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 179 562 799 1173
Size: 179 383 237 374
Fragments arranged by size:
383 374 237 179
BseYI C'CCAG_C
Cuts at: 0 46 592 1085 1150 1173
Size: 46 546 493 65 23
Fragments arranged by size:
546 493 65 46 23
BsiHKAI G_wGCw'C
Cuts at: 0 23 1173
Size: 23 1150
Bsp1286I G_dGCh'C
Cuts at: 0 23 141 1173
Size: 23 118 1032
Fragments arranged by size:
1032 118 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1100 1173
Size: 1100 73
BsrGI T'GTAC_A
Cuts at: 0 695 792 940 1173
Size: 695 97 148 233
Fragments arranged by size:
695 233 148 97
Bsu36I CC'TnA_GG
Cuts at: 0 307 1173
Size: 307 866
BtgI C'CryG_G
Cuts at: 0 95 1173
Size: 95 1078
BtsI GCAGTG_nn'
Cuts at: 0 147 181 1173
Size: 147 34 992
Fragments arranged by size:
992 147 34
EaeI y'GGCC_r
Cuts at: 0 98 654 1173
Size: 98 556 519
Fragments arranged by size:
556 519 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 1173
Size: 80 1093
EcoICRI GAG'CTC
Cuts at: 0 21 1173
Size: 21 1152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 341 475 1065 1173
Size: 79 262 134 590 108
Fragments arranged by size:
590 262 134 108 79
EcoO109I rG'GnC_Cy
Cuts at: 0 31 120 722 1054 1089 1147 1173
Size: 31 89 602 332 35 58 26
Fragments arranged by size:
602 332 89 58 35 31 26
HaeII r_GCGC'y
Cuts at: 0 58 1173
Size: 58 1115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 300 316 332 348 995 1027 1173
Size: 300 16 16 16 647 32 146
Fragments arranged by size:
647 300 146 32 16 16 16
HincII GTy'rAC
Cuts at: 0 435 1173
Size: 435 738
MslI CAynn'nnrTG
Cuts at: 0 164 251 1102 1173
Size: 164 87 851 71
Fragments arranged by size:
851 164 87 71
MspA1I CmG'CkG
Cuts at: 0 810 1085 1173
Size: 810 275 88
Fragments arranged by size:
810 275 88
NdeI CA'TA_TG
Cuts at: 0 1134 1173
Size: 1134 39
NheI G'CTAG_C
Cuts at: 0 579 1173
Size: 579 594
NspI r_CATG'y
Cuts at: 0 850 1173
Size: 850 323
PciI A'CATG_T
Cuts at: 0 846 1173
Size: 846 327
PfoI T'CCnGG_A
Cuts at: 0 318 1173
Size: 318 855
PpuMI rG'GwC_Cy
Cuts at: 0 31 722 1054 1089 1173
Size: 31 691 332 35 84
Fragments arranged by size:
691 332 84 35 31
PshAI GACnn'nnGTC
Cuts at: 0 200 1173
Size: 200 973
PvuII CAG'CTG
Cuts at: 0 1085 1173
Size: 1085 88
SacI G_AGCT'C
Cuts at: 0 23 1173
Size: 23 1150
SfcI C'TryA_G
Cuts at: 0 342 521 1070 1173
Size: 342 179 549 103
Fragments arranged by size:
549 342 179 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 796 1173
Size: 796 377
TatI w'GTAC_w
Cuts at: 0 695 792 940 1173
Size: 695 97 148 233
Fragments arranged by size:
695 233 148 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 856 888 1173
Size: 856 32 285
Fragments arranged by size:
856 285 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 540 814 1173
Size: 120 420 274 359
Fragments arranged by size:
420 359 274 120
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI AvaI BanI
BanII BciVI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I
BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrGI Bsu36I
BtgI BtsI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I HincII MslI MspA1I NdeI NheI NspI PciI
PfoI PpuMI PshAI PvuII SacI SfcI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI