Restriction map for
pF1KA0260
(Linear) (Six-base) MAPSORT of: KIAA0260.seq Check: 6908 from: 1 to: 1065
KIAA0260.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AatII G_ACGT'C
Cuts at: 0 21 1065
Size: 21 1044
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 467 1019 1065
Size: 142 325 552 46
Fragments arranged by size:
552 325 142 46
AseI AT'TA_AT
Cuts at: 0 883 1065
Size: 883 182
AvaI C'yCGr_G
Cuts at: 0 29 1065
Size: 29 1036
BanII G_rGCy'C
Cuts at: 0 157 1065
Size: 157 908
BbsI GAAGACnn'nnnn_
Cuts at: 0 472 1065
Size: 472 593
BglI GCCn_nnn'nGGC
Cuts at: 0 250 1065
Size: 250 815
BlpI GC'TnA_GC
Cuts at: 0 1009 1065
Size: 1009 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 1065
Size: 475 590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1065
Size: 445 620
BsaAI yAC'GTr
Cuts at: 0 612 929 1065
Size: 612 317 136
Fragments arranged by size:
612 317 136
BsaHI Gr'CG_yC
Cuts at: 0 18 94 1065
Size: 18 76 971
Fragments arranged by size:
971 76 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 506 536 1065
Size: 506 30 529
Fragments arranged by size:
529 506 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 94 201 1065
Size: 91 3 107 864
Fragments arranged by size:
864 107 91 3
BseYI C'CCAG_C
Cuts at: 0 84 639 746 964 1065
Size: 84 555 107 218 101
Fragments arranged by size:
555 218 107 101 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 1065
Size: 810 255
BsiEI CG_ry'CG
Cuts at: 0 101 1065
Size: 101 964
BsiHKAI G_wGCw'C
Cuts at: 0 157 191 1065
Size: 157 34 874
Fragments arranged by size:
874 157 34
Bsp1286I G_dGCh'C
Cuts at: 0 157 191 1065
Size: 157 34 874
Fragments arranged by size:
874 157 34
BspHI T'CATG_A
Cuts at: 0 677 1065
Size: 677 388
BsrDI GCAATG_nn'
Cuts at: 0 509 698 959 1065
Size: 509 189 261 106
Fragments arranged by size:
509 261 189 106
BsrFI r'CCGG_y
Cuts at: 0 136 1065
Size: 136 929
BstAPI GCAn_nnn'nTGC
Cuts at: 0 604 1065
Size: 604 461
DraI TTT'AAA
Cuts at: 0 950 1065
Size: 950 115
DraIII CAC_nnn'GTG
Cuts at: 0 220 1065
Size: 220 845
EaeI y'GGCC_r
Cuts at: 0 98 131 231 242 695 1065
Size: 98 33 100 11 453 370
Fragments arranged by size:
453 370 100 98 33 11
EagI C'GGCC_G
Cuts at: 0 98 1065
Size: 98 967
EarI CTCTTCn'nnn_
Cuts at: 0 995 1065
Size: 995 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1065
Size: 19 1046
EcoICRI GAG'CTC
Cuts at: 0 155 1065
Size: 155 910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 467 1019 1065
Size: 142 325 552 46
Fragments arranged by size:
552 325 142 46
HaeII r_GCGC'y
Cuts at: 0 277 1065
Size: 277 788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1065
Size: 204 861
MscI TGG'CCA
Cuts at: 0 233 244 697 1065
Size: 233 11 453 368
Fragments arranged by size:
453 368 233 11
MspA1I CmG'CkG
Cuts at: 0 1008 1065
Size: 1008 57
NaeI GCC'GGC
Cuts at: 0 138 1065
Size: 138 927
NgoMIV G'CCGG_C
Cuts at: 0 136 1065
Size: 136 929
NspI r_CATG'y
Cuts at: 0 28 1065
Size: 28 1037
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 1065
Size: 240 825
PmlI CAC'GTG
Cuts at: 0 929 1065
Size: 929 136
PstI C_TGCA'G
Cuts at: 0 773 1065
Size: 773 292
PvuII CAG'CTG
Cuts at: 0 1008 1065
Size: 1008 57
SacI G_AGCT'C
Cuts at: 0 157 1065
Size: 157 908
SapI GCTCTTCn'nnn_
Cuts at: 0 995 1065
Size: 995 70
ScaI AGT'ACT
Cuts at: 0 821 1065
Size: 821 244
SfcI C'TryA_G
Cuts at: 0 769 1065
Size: 769 296
SmlI C'TyrA_G
Cuts at: 0 460 1065
Size: 460 605
SnaBI TAC'GTA
Cuts at: 0 612 1065
Size: 612 453
SphI G_CATG'C
Cuts at: 0 28 1065
Size: 28 1037
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 252 1065
Size: 252 813
TatI w'GTAC_w
Cuts at: 0 819 1065
Size: 819 246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 1065
Size: 537 528
ZraI GAC'GTC
Cuts at: 0 19 1065
Size: 19 1046
Enzymes that do cut:
AatII AcuI AseI AvaI BanII BbsI BglI BlpI
BplI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstAPI DraI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI HaeII
MmeI MscI MspA1I NaeI NgoMIV NspI PflMI PmlI
PstI PvuII SacI SapI ScaI SfcI SmlI SnaBI
SphI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BsaI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DrdI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI