Restriction map for
pF1KSDA0262
(Linear) (Six-base) MAPSORT of: KIAA0262.seq Check: 678 from: 1 to: 2433
KIAA0262.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1005 2433
Size: 1005 1428
AccI GT'mk_AC
Cuts at: 0 944 2433
Size: 944 1489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 2277 2327 2378 2433
Size: 593 1684 50 51 55
Fragments arranged by size:
1684 593 55 51 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1943 2433
Size: 1943 490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 43 75 2433
Size: 43 32 2358
Fragments arranged by size:
2358 43 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1020 1065 1366 1378 1630 1724 2145 2433
Size: 1020 45 301 12 252 94 421 288
Fragments arranged by size:
1020 421 301 288 252 94 45 12
ApaI G_GGCC'C
Cuts at: 0 1998 2433
Size: 1998 435
ApoI r'AATT_y
Cuts at: 0 432 2286 2433
Size: 432 1854 147
Fragments arranged by size:
1854 432 147
AvaI C'yCGr_G
Cuts at: 0 1087 1130 1612 1823 1906 2433
Size: 1087 43 482 211 83 527
Fragments arranged by size:
1087 527 482 211 83 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 602 635 2433
Size: 602 33 1798
Fragments arranged by size:
1798 602 33
BamHI G'GATC_C
Cuts at: 0 175 2433
Size: 175 2258
BanII G_rGCy'C
Cuts at: 0 13 1124 1612 1998 2433
Size: 13 1111 488 386 435
Fragments arranged by size:
1111 488 435 386 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 348 581 778 2120 2433
Size: 348 233 197 1342 313
Fragments arranged by size:
1342 348 313 233 197
BbvCI CC'TCA_GC
Cuts at: 0 1498 2146 2433
Size: 1498 648 287
Fragments arranged by size:
1498 648 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2400 2433
Size: 2400 33
BfrBI ATG'CAT
Cuts at: 0 743 2433
Size: 743 1690
BglII A'GATC_T
Cuts at: 0 1619 2433
Size: 1619 814
BlpI GC'TnA_GC
Cuts at: 0 7 2433
Size: 7 2426
Bme1580I G_kGCm'C
Cuts at: 0 1998 2276 2433
Size: 1998 278 157
Fragments arranged by size:
1998 278 157
BmgBI CAC'GTC
Cuts at: 0 776 2433
Size: 776 1657
BmrI ACTGGGnnnn_n'
Cuts at: 0 384 633 2270 2433
Size: 384 249 1637 163
Fragments arranged by size:
1637 384 249 163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1638 1794 2433
Size: 1638 156 639
Fragments arranged by size:
1638 639 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 234 1057 1282 1621 2433
Size: 153 81 823 225 339 812
Fragments arranged by size:
823 812 339 225 153 81
Bpu10I CC'TnA_GC
Cuts at: 0 1498 2146 2433
Size: 1498 648 287
Fragments arranged by size:
1498 648 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1108 2433
Size: 808 300 1325
Fragments arranged by size:
1325 808 300
BsaI GGTCTCn'nnnn_
Cuts at: 0 1758 2433
Size: 1758 675
BsaBI GATnn'nnATC
Cuts at: 0 1424 2433
Size: 1424 1009
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 55 73 85 771 801 1255 1285
Size: 43 12 18 12 686 30 454 30
Cuts at: 1285 2384 2414 2433
Size: 1099 30 19
Fragments arranged by size:
1099 686 454 43 30 30 30 19
18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 278 1054 1087 1366 1620 1876 1941
Size: 60 218 776 33 279 254 256 65
Cuts at: 1941 2000 2433
Size: 59 433
Fragments arranged by size:
776 433 279 256 254 218 65 60
59 33
BseYI C'CCAG_C
Cuts at: 0 491 734 2433
Size: 491 243 1699
Fragments arranged by size:
1699 491 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 1006 1540 2433
Size: 733 273 534 893
Fragments arranged by size:
893 733 534 273
BsiHKAI G_wGCw'C
Cuts at: 0 13 1124 2433
Size: 13 1111 1309
Fragments arranged by size:
1309 1111 13
Bsp1286I G_dGCh'C
Cuts at: 0 13 1124 1612 1998 2276 2433
Size: 13 1111 488 386 278 157
Fragments arranged by size:
1111 488 386 278 157 13
BspHI T'CATG_A
Cuts at: 0 2318 2433
Size: 2318 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1005 1954 2433
Size: 1005 949 479
Fragments arranged by size:
1005 949 479
BsrBI CCG'CTC
Cuts at: 0 113 2433
Size: 113 2320
BsrFI r'CCGG_y
Cuts at: 0 133 2433
Size: 133 2300
BssSI C'ACGA_G
Cuts at: 0 1579 2433
Size: 1579 854
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1020 2433
Size: 1020 1413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 397 2433
Size: 397 2036
BstYI r'GATC_y
Cuts at: 0 175 819 1619 2346 2433
Size: 175 644 800 727 87
Fragments arranged by size:
800 727 644 175 87
Bsu36I CC'TnA_GG
Cuts at: 0 1379 2433
Size: 1379 1054
BtgI C'CryG_G
Cuts at: 0 450 1292 1989 2433
Size: 450 842 697 444
Fragments arranged by size:
842 697 450 444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 170 2433
Size: 170 2263
BtsI GCAGTG_nn'
Cuts at: 0 483 1367 2066 2433
Size: 483 884 699 367
Fragments arranged by size:
884 699 483 367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1616 1651 2433
Size: 1616 35 782
Fragments arranged by size:
1616 782 35
DraIII CAC_nnn'GTG
Cuts at: 0 766 1563 2433
Size: 766 797 870
Fragments arranged by size:
870 797 766
DrdI GACnn_nn'nnGTC
Cuts at: 0 2058 2433
Size: 2058 375
EaeI y'GGCC_r
Cuts at: 0 453 1154 2433
Size: 453 701 1279
Fragments arranged by size:
1279 701 453
EarI CTCTTCn'nnn_
Cuts at: 0 84 330 886 1130 1343 1789 2354 2433
Size: 84 246 556 244 213 446 565 79
Fragments arranged by size:
565 556 446 246 244 213 84 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 63 105 2433
Size: 63 42 2328
Fragments arranged by size:
2328 63 42
EcoICRI GAG'CTC
Cuts at: 0 11 1122 2433
Size: 11 1111 1311
Fragments arranged by size:
1311 1111 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 234 593 1057 1282 1621 2277 2327
Size: 153 81 359 464 225 339 656 50
Cuts at: 2327 2378 2433
Size: 51 55
Fragments arranged by size:
656 464 359 339 225 153 81 55
51 50
EcoO109I rG'GnC_Cy
Cuts at: 0 1969 1994 1995 2433
Size: 1969 25 1 438
Fragments arranged by size:
1969 438 25 1
EcoRV GAT'ATC
Cuts at: 0 1687 2433
Size: 1687 746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 287 555 1503 2433
Size: 287 268 948 930
Fragments arranged by size:
948 930 287 268
FspI TGC'GCA
Cuts at: 0 646 2433
Size: 646 1787
FspAI rTGC'GCAy
Cuts at: 0 646 2433
Size: 646 1787
HaeII r_GCGC'y
Cuts at: 0 1812 2433
Size: 1812 621
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 771 803 1129 1161 1255 1287
Size: 273 32 466 32 326 32 94 32
Cuts at: 1287 1788 1820 2338 2370 2433
Size: 501 32 518 32 63
Fragments arranged by size:
518 501 466 326 273 94 63 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 621 2433
Size: 621 1812
HpaI GTT'AAC
Cuts at: 0 621 2433
Size: 621 1812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 86 2133 2433
Size: 62 24 2047 300
Fragments arranged by size:
2047 300 62 24
MscI TGG'CCA
Cuts at: 0 455 2433
Size: 455 1978
MslI CAynn'nnrTG
Cuts at: 0 854 877 2433
Size: 854 23 1556
Fragments arranged by size:
1556 854 23
MspA1I CmG'CkG
Cuts at: 0 6 56 885 1065 2433
Size: 6 50 829 180 1368
Fragments arranged by size:
1368 829 180 50 6
NaeI GCC'GGC
Cuts at: 0 135 2433
Size: 135 2298
NcoI C'CATG_G
Cuts at: 0 1989 2433
Size: 1989 444
NdeI CA'TA_TG
Cuts at: 0 1547 2433
Size: 1547 886
NgoMIV G'CCGG_C
Cuts at: 0 133 2433
Size: 133 2300
NsiI A_TGCA'T
Cuts at: 0 745 2433
Size: 745 1688
NspI r_CATG'y
Cuts at: 0 743 2433
Size: 743 1690
PflMI CCAn_nnn'nTGG
Cuts at: 0 564 2433
Size: 564 1869
PfoI T'CCnGG_A
Cuts at: 0 1115 2433
Size: 1115 1318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 2433
Size: 43 32 2358
Fragments arranged by size:
2358 43 32
PpuMI rG'GwC_Cy
Cuts at: 0 1969 2433
Size: 1969 464
PsiI TTA'TAA
Cuts at: 0 188 2433
Size: 188 2245
PspOMI G'GGCC_C
Cuts at: 0 1994 2433
Size: 1994 439
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 527 559 2433
Size: 527 32 1874
Fragments arranged by size:
1874 527 32
PstI C_TGCA'G
Cuts at: 0 702 2433
Size: 702 1731
PvuII CAG'CTG
Cuts at: 0 885 1065 2433
Size: 885 180 1368
Fragments arranged by size:
1368 885 180
SacI G_AGCT'C
Cuts at: 0 13 1124 2433
Size: 13 1111 1309
Fragments arranged by size:
1309 1111 13
SanDI GG'GwC_CC
Cuts at: 0 1969 2433
Size: 1969 464
SapI GCTCTTCn'nnn_
Cuts at: 0 330 2433
Size: 330 2103
ScaI AGT'ACT
Cuts at: 0 985 2433
Size: 985 1448
SfcI C'TryA_G
Cuts at: 0 698 1915 2433
Size: 698 1217 518
Fragments arranged by size:
1217 698 518
SmlI C'TyrA_G
Cuts at: 0 823 1123 1906 2433
Size: 823 300 783 527
Fragments arranged by size:
823 783 527 300
SphI G_CATG'C
Cuts at: 0 743 2433
Size: 743 1690
StuI AGG'CCT
Cuts at: 0 1396 2433
Size: 1396 1037
StyI C'CwwG_G
Cuts at: 0 1220 1989 2433
Size: 1220 769 444
Fragments arranged by size:
1220 769 444
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2079 2433
Size: 2079 354
TatI w'GTAC_w
Cuts at: 0 983 2433
Size: 983 1450
Tth111I GACn'n_nGTC
Cuts at: 0 1676 2433
Size: 1676 757
XbaI T'CTAG_A
Cuts at: 0 1321 2433
Size: 1321 1112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 996 1534 2433
Size: 996 538 899
Fragments arranged by size:
996 899 538
XhoI C'TCGA_G
Cuts at: 0 1906 2433
Size: 1906 527
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApaI ApoI
AvaI BaeI BamHI BanII BbsI BbvCI BcgI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI
FspI FspAI HaeII Hin4I HincII HpaI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI
PflMI PfoI PpiI PpuMI PsiI PspOMI PsrI PstI
PvuII SacI SanDI SapI ScaI SfcI SmlI SphI
StuI StyI TaqII TatI Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaLI AscI AseI AsiSI AvrII BanI BbeI
BciVI BclI BglI BmtI BsaAI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I ClaI DraI EagI EcoNI EcoRI FseI
HindIII KasI KpnI MfeI MluI NarI NheI NotI
NruI PacI PasI PciI PmeI PmlI PshAI PspXI
PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI XmaI XmnI ZraI