Restriction map for
pF1KSDA0263
(Linear) (Six-base) MAPSORT of: KIAA0263.seq Check: 6577 from: 1 to: 1323
KIAA0263.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 780 1323
Size: 780 543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1323
Size: 694 629
AfeI AGC'GCT
Cuts at: 0 169 1323
Size: 169 1154
AflIII A'CryG_T
Cuts at: 0 704 1265 1323
Size: 704 561 58
Fragments arranged by size:
704 561 58
AgeI A'CCGG_T
Cuts at: 0 368 1323
Size: 368 955
AhdI GACnn_n'nnGTC
Cuts at: 0 1056 1323
Size: 1056 267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 133 1323
Size: 133 1190
AlwNI CAG_nnn'CTG
Cuts at: 0 573 779 1323
Size: 573 206 544
Fragments arranged by size:
573 544 206
ApaLI G'TGCA_C
Cuts at: 0 640 1323
Size: 640 683
AvaI C'yCGr_G
Cuts at: 0 158 1147 1323
Size: 158 989 176
Fragments arranged by size:
989 176 158
BanII G_rGCy'C
Cuts at: 0 83 436 851 1323
Size: 83 353 415 472
Fragments arranged by size:
472 415 353 83
BlpI GC'TnA_GC
Cuts at: 0 574 1323
Size: 574 749
Bme1580I G_kGCm'C
Cuts at: 0 644 685 1323
Size: 644 41 638
Fragments arranged by size:
644 638 41
BmgBI CAC'GTC
Cuts at: 0 913 1323
Size: 913 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 97 567 978 985 1297 1323
Size: 77 20 470 411 7 312 26
Fragments arranged by size:
470 411 312 77 26 20 7
Bpu10I CC'TnA_GC
Cuts at: 0 934 1323
Size: 934 389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 537 649 1057 1323
Size: 439 98 112 408 266
Fragments arranged by size:
439 408 266 112 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 53 415 1323
Size: 53 362 908
Fragments arranged by size:
908 362 53
BsaAI yAC'GTr
Cuts at: 0 1266 1323
Size: 1266 57
BsaHI Gr'CG_yC
Cuts at: 0 770 1105 1323
Size: 770 335 218
Fragments arranged by size:
770 335 218
BsaWI w'CCGG_w
Cuts at: 0 368 1323
Size: 368 955
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 540 570 1323
Size: 540 30 753
Fragments arranged by size:
753 540 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 1152 1323
Size: 412 740 171
Fragments arranged by size:
740 412 171
BseYI C'CCAG_C
Cuts at: 0 765 1120 1132 1323
Size: 765 355 12 191
Fragments arranged by size:
765 355 191 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 451 874 1323
Size: 349 102 423 449
Fragments arranged by size:
449 423 349 102
BsiHKAI G_wGCw'C
Cuts at: 0 436 644 754 1094 1323
Size: 436 208 110 340 229
Fragments arranged by size:
436 340 229 208 110
BsmI GAATG_Cn'
Cuts at: 0 48 1323
Size: 48 1275
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1075 1323
Size: 1075 248
Bsp1286I G_dGCh'C
Cuts at: 0 83 436 644 685 754 851 1094 1323
Size: 83 353 208 41 69 97 243 229
Fragments arranged by size:
353 243 229 208 97 83 69 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 780 914 1323
Size: 780 134 409
Fragments arranged by size:
780 409 134
BsrBI CCG'CTC
Cuts at: 0 969 1323
Size: 969 354
BsrDI GCAATG_nn'
Cuts at: 0 876 1323
Size: 876 447
BsrFI r'CCGG_y
Cuts at: 0 368 1055 1323
Size: 368 687 268
Fragments arranged by size:
687 368 268
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1028 1323
Size: 1028 295
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 1323
Size: 24 1299
Bsu36I CC'TnA_GG
Cuts at: 0 1097 1323
Size: 1097 226
BtgI C'CryG_G
Cuts at: 0 17 843 1323
Size: 17 826 480
Fragments arranged by size:
826 480 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 713 1323
Size: 713 610
BtsI GCAGTG_nn'
Cuts at: 0 384 390 1323
Size: 384 6 933
Fragments arranged by size:
933 384 6
DraIII CAC_nnn'GTG
Cuts at: 0 138 140 1323
Size: 138 2 1183
Fragments arranged by size:
1183 138 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 67 1323
Size: 67 1256
EaeI y'GGCC_r
Cuts at: 0 840 1323
Size: 840 483
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 1323
Size: 116 1207
EcoICRI GAG'CTC
Cuts at: 0 434 1323
Size: 434 889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 97 567 694 978 985 1297 1323
Size: 77 20 470 127 284 7 312 26
Fragments arranged by size:
470 312 284 127 77 26 20 7
EcoO109I rG'GnC_Cy
Cuts at: 0 483 948 1156 1323
Size: 483 465 208 167
Fragments arranged by size:
483 465 208 167
HaeII r_GCGC'y
Cuts at: 0 171 1323
Size: 171 1152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 1042 1074 1090 1122 1323
Size: 691 32 319 32 16 32 201
Fragments arranged by size:
691 319 201 32 32 32 16
MluI A'CGCG_T
Cuts at: 0 704 1323
Size: 704 619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1323
Size: 647 676
MslI CAynn'nnrTG
Cuts at: 0 22 698 790 1323
Size: 22 676 92 533
Fragments arranged by size:
676 533 92 22
MspA1I CmG'CkG
Cuts at: 0 573 712 801 1323
Size: 573 139 89 522
Fragments arranged by size:
573 522 139 89
NcoI C'CATG_G
Cuts at: 0 17 1323
Size: 17 1306
NspI r_CATG'y
Cuts at: 0 585 789 1323
Size: 585 204 534
Fragments arranged by size:
585 534 204
PfoI T'CCnGG_A
Cuts at: 0 74 1223 1323
Size: 74 1149 100
Fragments arranged by size:
1149 100 74
PpuMI rG'GwC_Cy
Cuts at: 0 483 1156 1323
Size: 483 673 167
Fragments arranged by size:
673 483 167
PstI C_TGCA'G
Cuts at: 0 826 1323
Size: 826 497
PvuII CAG'CTG
Cuts at: 0 573 712 801 1323
Size: 573 139 89 522
Fragments arranged by size:
573 522 139 89
SacI G_AGCT'C
Cuts at: 0 436 1323
Size: 436 887
SanDI GG'GwC_CC
Cuts at: 0 1156 1323
Size: 1156 167
ScaI AGT'ACT
Cuts at: 0 835 1323
Size: 835 488
SfcI C'TryA_G
Cuts at: 0 822 1323
Size: 822 501
SmaI CCC'GGG
Cuts at: 0 160 1323
Size: 160 1163
SmlI C'TyrA_G
Cuts at: 0 418 516 628 1072 1323
Size: 418 98 112 444 251
Fragments arranged by size:
444 418 251 112 98
SphI G_CATG'C
Cuts at: 0 585 789 1323
Size: 585 204 534
Fragments arranged by size:
585 534 204
StuI AGG'CCT
Cuts at: 0 976 1323
Size: 976 347
StyI C'CwwG_G
Cuts at: 0 17 596 1172 1323
Size: 17 579 576 151
Fragments arranged by size:
579 576 151 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 617 1323
Size: 617 706
TatI w'GTAC_w
Cuts at: 0 833 1323
Size: 833 490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 499 1323
Size: 499 824
XmaI C'CCGG_G
Cuts at: 0 158 1323
Size: 158 1165
XmnI GAAnn'nnTTC
Cuts at: 0 202 1323
Size: 202 1121
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AhdI AlfI AlwNI
ApaLI AvaI BanII BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
MluI MmeI MslI MspA1I NcoI NspI PfoI PpuMI
PstI PvuII SacI SanDI ScaI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AleI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmrI BmtI BplI BsaBI BsiEI BsiWI BspEI
BspHI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI EagI EarI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI
Tth111I XbaI XhoI ZraI