Restriction map for
pF1KSDA0264
(Linear) (Six-base) MAPSORT of: KIAA0264.seq Check: 1765 from: 1 to: 1242
KIAA0264.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 104 1242
Size: 104 1138
Acc65I G'GTAC_C
Cuts at: 0 305 1242
Size: 305 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 570 857 872 880 1242
Size: 77 493 287 15 8 362
Fragments arranged by size:
493 362 287 77 15 8
AfeI AGC'GCT
Cuts at: 0 836 1242
Size: 836 406
AlwNI CAG_nnn'CTG
Cuts at: 0 1134 1242
Size: 1134 108
ApoI r'AATT_y
Cuts at: 0 407 1242
Size: 407 835
AvaI C'yCGr_G
Cuts at: 0 242 1242
Size: 242 1000
BanI G'GyrC_C
Cuts at: 0 305 1242
Size: 305 937
BanII G_rGCy'C
Cuts at: 0 777 1242
Size: 777 465
BbsI GAAGACnn'nnnn_
Cuts at: 0 583 818 920 1242
Size: 583 235 102 322
Fragments arranged by size:
583 322 235 102
BbvCI CC'TCA_GC
Cuts at: 0 56 1242
Size: 56 1186
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 1242
Size: 663 579
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 853 1242
Size: 853 389
Bpu10I CC'TnA_GC
Cuts at: 0 56 1242
Size: 56 1186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 907 1242
Size: 613 294 335
Fragments arranged by size:
613 335 294
BseYI C'CCAG_C
Cuts at: 0 1070 1242
Size: 1070 172
Bsp1286I G_dGCh'C
Cuts at: 0 777 1242
Size: 777 465
BspHI T'CATG_A
Cuts at: 0 500 1242
Size: 500 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 61 92 1242
Size: 61 31 1150
Fragments arranged by size:
1150 61 31
BsrDI GCAATG_nn'
Cuts at: 0 561 1242
Size: 561 681
BsrGI T'GTAC_A
Cuts at: 0 1158 1242
Size: 1158 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1134 1242
Size: 1134 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 676 1242
Size: 676 566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1098 1242
Size: 1098 144
BtsI GCAGTG_nn'
Cuts at: 0 349 860 1147 1242
Size: 349 511 287 95
Fragments arranged by size:
511 349 287 95
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1056 1091 1242
Size: 1056 35 151
Fragments arranged by size:
1056 151 35
EarI CTCTTCn'nnn_
Cuts at: 0 242 590 1242
Size: 242 348 652
Fragments arranged by size:
652 348 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 570 857 872 880 1242
Size: 77 493 287 15 8 362
Fragments arranged by size:
493 362 287 77 15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 336 1242
Size: 336 906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 67 600 1242
Size: 67 533 642
Fragments arranged by size:
642 533 67
HaeII r_GCGC'y
Cuts at: 0 838 1242
Size: 838 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 466 498 883 915 1242
Size: 145 32 289 32 385 32 327
Fragments arranged by size:
385 327 289 145 32 32 32
HindIII A'AGCT_T
Cuts at: 0 976 1018 1242
Size: 976 42 224
Fragments arranged by size:
976 224 42
KpnI G_GTAC'C
Cuts at: 0 309 1242
Size: 309 933
MspA1I CmG'CkG
Cuts at: 0 877 1242
Size: 877 365
NspI r_CATG'y
Cuts at: 0 741 1242
Size: 741 501
PflMI CCAn_nnn'nTGG
Cuts at: 0 302 1242
Size: 302 940
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 380 412 1242
Size: 380 32 830
Fragments arranged by size:
830 380 32
PstI C_TGCA'G
Cuts at: 0 72 1139 1242
Size: 72 1067 103
Fragments arranged by size:
1067 103 72
PvuII CAG'CTG
Cuts at: 0 877 1242
Size: 877 365
SbfI CC_TGCA'GG
Cuts at: 0 72 1242
Size: 72 1170
SfcI C'TryA_G
Cuts at: 0 68 1135 1242
Size: 68 1067 107
Fragments arranged by size:
1067 107 68
StuI AGG'CCT
Cuts at: 0 635 1006 1242
Size: 635 371 236
Fragments arranged by size:
635 371 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 547 1084 1242
Size: 547 537 158
Fragments arranged by size:
547 537 158
TatI w'GTAC_w
Cuts at: 0 1158 1242
Size: 1158 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 597 629 1242
Size: 597 32 613
Fragments arranged by size:
613 597 32
XbaI T'CTAG_A
Cuts at: 0 1149 1242
Size: 1149 93
Enzymes that do cut:
AccI Acc65I AcuI AfeI AlwNI ApoI AvaI BanI
BanII BbsI BbvCI BmrI BplI Bpu10I BseRI BseYI
Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstXI BtgZI
BtsI CspCI EarI Eco57MI EcoNI FalI HaeII Hin4I
HindIII KpnI MspA1I NspI PflMI PsrI PstI PvuII
SbfI SfcI StuI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI ClaI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I
XcmI XhoI XmaI XmnI ZraI