Restriction map for
pF1KSDA0269
(Linear) (Six-base) MAPSORT of: KIAA0269.seq Check: 272 from: 1 to: 1677
KIAA0269.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 174 463 662 1108 1677
Size: 164 10 289 199 446 569
Fragments arranged by size:
569 446 289 199 164 10
AflIII A'CryG_T
Cuts at: 0 213 1677
Size: 213 1464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 1677
Size: 364 1313
AlwNI CAG_nnn'CTG
Cuts at: 0 618 1081 1266 1677
Size: 618 463 185 411
Fragments arranged by size:
618 463 411 185
ApoI r'AATT_y
Cuts at: 0 1651 1677
Size: 1651 26
AseI AT'TA_AT
Cuts at: 0 1405 1677
Size: 1405 272
AvrII C'CTAG_G
Cuts at: 0 28 1677
Size: 28 1649
BanII G_rGCy'C
Cuts at: 0 964 1677
Size: 964 713
BbvCI CC'TCA_GC
Cuts at: 0 800 1677
Size: 800 877
BfrBI ATG'CAT
Cuts at: 0 852 1677
Size: 852 825
BlpI GC'TnA_GC
Cuts at: 0 1565 1677
Size: 1565 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 1207 1677
Size: 1207 470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 983 1677
Size: 983 694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1058 1079 1097 1157 1677
Size: 1034 24 21 18 60 520
Fragments arranged by size:
1034 520 60 24 21 18
Bpu10I CC'TnA_GC
Cuts at: 0 800 1677
Size: 800 877
BsaAI yAC'GTr
Cuts at: 0 726 1677
Size: 726 951
BsaBI GATnn'nnATC
Cuts at: 0 653 1677
Size: 653 1024
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1023 1053 1677
Size: 1023 30 624
Fragments arranged by size:
1023 624 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 968 1031 1109 1145 1148 1163 1280 1409
Size: 968 63 78 36 3 15 117 129
Cuts at: 1409 1677
Size: 268
Fragments arranged by size:
968 268 129 117 78 63 36 15
3
BseYI C'CCAG_C
Cuts at: 0 690 1149 1677
Size: 690 459 528
Fragments arranged by size:
690 528 459
BsiEI CG_ry'CG
Cuts at: 0 339 1677
Size: 339 1338
BsiHKAI G_wGCw'C
Cuts at: 0 588 1677
Size: 588 1089
BsiWI C'GTAC_G
Cuts at: 0 371 1677
Size: 371 1306
BsmI GAATG_Cn'
Cuts at: 0 1307 1677
Size: 1307 370
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 1677
Size: 361 1316
Bsp1286I G_dGCh'C
Cuts at: 0 588 964 1677
Size: 588 376 713
Fragments arranged by size:
713 588 376
BspHI T'CATG_A
Cuts at: 0 561 591 1677
Size: 561 30 1086
Fragments arranged by size:
1086 561 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1446 1677
Size: 1446 231
BsrDI GCAATG_nn'
Cuts at: 0 202 1677
Size: 202 1475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 865 1677
Size: 865 812
BstYI r'GATC_y
Cuts at: 0 742 1677
Size: 742 935
BtsI GCAGTG_nn'
Cuts at: 0 49 1079 1677
Size: 49 1030 598
Fragments arranged by size:
1030 598 49
ClaI AT'CG_AT
Cuts at: 0 23 1677
Size: 23 1654
DraIII CAC_nnn'GTG
Cuts at: 0 1252 1677
Size: 1252 425
EarI CTCTTCn'nnn_
Cuts at: 0 995 1127 1538 1677
Size: 995 132 411 139
Fragments arranged by size:
995 411 139 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 174 463 662 1034 1058 1079 1097
Size: 164 10 289 199 372 24 21 18
Cuts at: 1097 1108 1157 1677
Size: 11 49 520
Fragments arranged by size:
520 372 289 199 164 49 24 21
18 11 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1390 1677
Size: 1390 287
HaeII r_GCGC'y
Cuts at: 0 1458 1677
Size: 1458 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 900 932 1023 1055 1677
Size: 211 32 657 32 91 32 622
Fragments arranged by size:
657 622 211 91 32 32 32
HincII GTy'rAC
Cuts at: 0 198 1007 1677
Size: 198 809 670
Fragments arranged by size:
809 670 198
HindIII A'AGCT_T
Cuts at: 0 296 1677
Size: 296 1381
MfeI C'AATT_G
Cuts at: 0 1163 1677
Size: 1163 514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1652 1677
Size: 1393 259 25
Fragments arranged by size:
1393 259 25
MslI CAynn'nnrTG
Cuts at: 0 737 1677
Size: 737 940
NdeI CA'TA_TG
Cuts at: 0 734 1677
Size: 734 943
NsiI A_TGCA'T
Cuts at: 0 854 1677
Size: 854 823
PflMI CCAn_nnn'nTGG
Cuts at: 0 853 1677
Size: 853 824
PpuMI rG'GwC_Cy
Cuts at: 0 1390 1677
Size: 1390 287
PvuI CG_AT'CG
Cuts at: 0 339 1677
Size: 339 1338
SapI GCTCTTCn'nnn_
Cuts at: 0 1538 1677
Size: 1538 139
SfcI C'TryA_G
Cuts at: 0 899 935 1677
Size: 899 36 742
Fragments arranged by size:
899 742 36
SspI AAT'ATT
Cuts at: 0 93 1677
Size: 93 1584
StyI C'CwwG_G
Cuts at: 0 28 98 624 1677
Size: 28 70 526 1053
Fragments arranged by size:
1053 526 70 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1163 1677
Size: 1163 514
TatI w'GTAC_w
Cuts at: 0 1179 1677
Size: 1179 498
XbaI T'CTAG_A
Cuts at: 0 549 1677
Size: 549 1128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 96 1677
Size: 96 1581
XmnI GAAnn'nnTTC
Cuts at: 0 298 1677
Size: 298 1379
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI AseI AvrII BanII
BbvCI BfrBI BlpI BmrI BplI BpmI Bpu10I BsaAI
BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BstAPI BstYI BtsI
ClaI DraIII EarI Eco57MI EcoO109I HaeII Hin4I HincII
HindIII MfeI MmeI MslI NdeI NsiI PflMI PpuMI
PvuI SapI SfcI SspI StyI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI
BglI BglII Bme1580I BmgBI BmtI BpuEI BsaI BsaHI
BsaWI BsgI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI CspCI
DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HpaI KasI KpnI
MluI MscI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TstI Tth111I XhoI
XmaI ZraI