Restriction map for
pF1KSDA0271
(Linear) (Six-base) MAPSORT of: KIAA0271.seq Check: 3310 from: 1 to: 579
KIAA0271.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AleI CACnn'nnGTG
Cuts at: 0 419 579
Size: 419 160
AlwNI CAG_nnn'CTG
Cuts at: 0 115 579
Size: 115 464
ApaI G_GGCC'C
Cuts at: 0 109 271 465 546 579
Size: 109 162 194 81 33
Fragments arranged by size:
194 162 109 81 33
AvaI C'yCGr_G
Cuts at: 0 97 579
Size: 97 482
BamHI G'GATC_C
Cuts at: 0 410 579
Size: 410 169
BanII G_rGCy'C
Cuts at: 0 36 109 271 465 546 579
Size: 36 73 162 194 81 33
Fragments arranged by size:
194 162 81 73 36 33
BbvCI CC'TCA_GC
Cuts at: 0 64 579
Size: 64 515
BglI GCCn_nnn'nGGC
Cuts at: 0 139 579
Size: 139 440
BlpI GC'TnA_GC
Cuts at: 0 197 219 579
Size: 197 22 360
Fragments arranged by size:
360 197 22
Bme1580I G_kGCm'C
Cuts at: 0 109 271 465 546 579
Size: 109 162 194 81 33
Fragments arranged by size:
194 162 109 81 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 507 547 579
Size: 285 222 40 32
Fragments arranged by size:
285 222 40 32
BmtI G_CTAG'C
Cuts at: 0 575 579
Size: 575 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 409 487 579
Size: 167 242 78 92
Fragments arranged by size:
242 167 92 78
Bpu10I CC'TnA_GC
Cuts at: 0 64 579
Size: 64 515
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 249 579
Size: 154 95 330
Fragments arranged by size:
330 154 95
BsaHI Gr'CG_yC
Cuts at: 0 480 579
Size: 480 99
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 579
Size: 484 95
BseYI C'CCAG_C
Cuts at: 0 9 108 428 579
Size: 9 99 320 151
Fragments arranged by size:
320 151 99 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 296 385 579
Size: 109 187 89 194
Fragments arranged by size:
194 187 109 89
BsmBI CGTCTCn'nnnn_
Cuts at: 0 385 579
Size: 385 194
Bsp1286I G_dGCh'C
Cuts at: 0 36 109 271 465 546 579
Size: 36 73 162 194 81 33
Fragments arranged by size:
194 162 81 73 36 33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 319 579
Size: 319 260
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 579
Size: 421 158
BstYI r'GATC_y
Cuts at: 0 410 579
Size: 410 169
BtgI C'CryG_G
Cuts at: 0 530 579
Size: 530 49
BtsI GCAGTG_nn'
Cuts at: 0 426 579
Size: 426 153
DraIII CAC_nnn'GTG
Cuts at: 0 319 579
Size: 319 260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 448 579
Size: 448 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 409 487 579
Size: 167 242 78 92
Fragments arranged by size:
242 167 92 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 469 579
Size: 469 110
EcoO109I rG'GnC_Cy
Cuts at: 0 105 267 268 461 462 542 543 560
Size: 105 162 1 193 1 80 1 17
Cuts at: 560 579
Size: 19
Fragments arranged by size:
193 162 105 80 19 17 1 1
1
HincII GTy'rAC
Cuts at: 0 333 579
Size: 333 246
MslI CAynn'nnrTG
Cuts at: 0 419 579
Size: 419 160
MspA1I CmG'CkG
Cuts at: 0 115 123 145 201 579
Size: 115 8 22 56 378
Fragments arranged by size:
378 115 56 22 8
NheI G'CTAG_C
Cuts at: 0 571 579
Size: 571 8
NspI r_CATG'y
Cuts at: 0 208 579
Size: 208 371
PsiI TTA'TAA
Cuts at: 0 59 579
Size: 59 520
PspOMI G'GGCC_C
Cuts at: 0 105 267 461 542 579
Size: 105 162 194 81 37
Fragments arranged by size:
194 162 105 81 37
PvuII CAG'CTG
Cuts at: 0 115 145 201 579
Size: 115 30 56 378
Fragments arranged by size:
378 115 56 30
SfcI C'TryA_G
Cuts at: 0 554 579
Size: 554 25
SmaI CCC'GGG
Cuts at: 0 99 579
Size: 99 480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 579
Size: 253 326
XmaI C'CCGG_G
Cuts at: 0 97 579
Size: 97 482
XmnI GAAnn'nnTTC
Cuts at: 0 257 579
Size: 257 322
Enzymes that do cut:
AleI AlwNI ApaI AvaI BamHI BanII BbvCI BglI
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaHI
BseRI BseYI BsgI BsmBI Bsp1286I BstAPI BstXI BstYI
BtgI BtsI DraIII EciI Eco57MI EcoNI EcoO109I HincII
MslI MspA1I NheI NspI PsiI PspOMI PvuII SfcI
SmaI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BmgBI BplI BpuEI BsaAI
BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EarI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI