Restriction map for
pF1KSDA0273
(Linear) (Six-base) MAPSORT of: KIAA0273.seq Check: 380 from: 1 to: 990
KIAA0273.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 55 990
Size: 55 935
AatII G_ACGT'C
Cuts at: 0 170 990
Size: 170 820
AccI GT'mk_AC
Cuts at: 0 875 931 990
Size: 875 56 59
Fragments arranged by size:
875 59 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 511 534 990
Size: 352 159 23 456
Fragments arranged by size:
456 352 159 23
ApoI r'AATT_y
Cuts at: 0 141 990
Size: 141 849
BanI G'GyrC_C
Cuts at: 0 717 889 911 990
Size: 717 172 22 79
Fragments arranged by size:
717 172 79 22
BanII G_rGCy'C
Cuts at: 0 238 872 990
Size: 238 634 118
Fragments arranged by size:
634 238 118
BbeI G_GCGC'C
Cuts at: 0 721 990
Size: 721 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 408 537 816 990
Size: 408 129 279 174
Fragments arranged by size:
408 279 174 129
BlpI GC'TnA_GC
Cuts at: 0 362 380 629 990
Size: 362 18 249 361
Fragments arranged by size:
362 361 249 18
Bme1580I G_kGCm'C
Cuts at: 0 892 914 990
Size: 892 22 76
Fragments arranged by size:
892 76 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 990
Size: 344 646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 330 871 990
Size: 112 218 541 119
Fragments arranged by size:
541 218 119 112
Bpu10I CC'TnA_GC
Cuts at: 0 232 990
Size: 232 758
BsaHI Gr'CG_yC
Cuts at: 0 167 718 990
Size: 167 551 272
Fragments arranged by size:
551 272 167
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 990
Size: 25 965
BseYI C'CCAG_C
Cuts at: 0 624 646 955 990
Size: 624 22 309 35
Fragments arranged by size:
624 309 35 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 315 990
Size: 283 32 675
Fragments arranged by size:
675 283 32
BsiEI CG_ry'CG
Cuts at: 0 606 756 990
Size: 606 150 234
Fragments arranged by size:
606 234 150
BsiHKAI G_wGCw'C
Cuts at: 0 356 990
Size: 356 634
BsmBI CGTCTCn'nnnn_
Cuts at: 0 310 990
Size: 310 680
Bsp1286I G_dGCh'C
Cuts at: 0 238 356 872 892 914 990
Size: 238 118 516 20 22 76
Fragments arranged by size:
516 238 118 76 22 20
BspHI T'CATG_A
Cuts at: 0 920 990
Size: 920 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 55 805 976 990
Size: 55 750 171 14
Fragments arranged by size:
750 171 55 14
BsrDI GCAATG_nn'
Cuts at: 0 773 990
Size: 773 217
BsrGI T'GTAC_A
Cuts at: 0 683 990
Size: 683 307
BssSI C'ACGA_G
Cuts at: 0 181 990
Size: 181 809
BstAPI GCAn_nnn'nTGC
Cuts at: 0 968 990
Size: 968 22
BstEII G'GTnAC_C
Cuts at: 0 99 990
Size: 99 891
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 990
Size: 183 807
BstYI r'GATC_y
Cuts at: 0 620 990
Size: 620 370
BtgI C'CryG_G
Cuts at: 0 74 510 537 990
Size: 74 436 27 453
Fragments arranged by size:
453 436 74 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 299 990
Size: 299 691
BtsI GCAGTG_nn'
Cuts at: 0 199 200 278 462 990
Size: 199 1 78 184 528
Fragments arranged by size:
528 199 184 78 1
EaeI y'GGCC_r
Cuts at: 0 185 257 603 990
Size: 185 72 346 387
Fragments arranged by size:
387 346 185 72
EagI C'GGCC_G
Cuts at: 0 603 990
Size: 603 387
EarI CTCTTCn'nnn_
Cuts at: 0 643 990
Size: 643 347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 990
Size: 817 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 330 352 511 534 871 990
Size: 112 218 22 159 23 337 119
Fragments arranged by size:
337 218 159 119 112 23 22
EcoO109I rG'GnC_Cy
Cuts at: 0 458 840 951 990
Size: 458 382 111 39
Fragments arranged by size:
458 382 111 39
EcoRI G'AATT_C
Cuts at: 0 141 990
Size: 141 849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 459 990
Size: 459 531
HaeII r_GCGC'y
Cuts at: 0 615 636 721 990
Size: 615 21 85 269
Fragments arranged by size:
615 269 85 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 612 644 990
Size: 27 585 32 346
Fragments arranged by size:
585 346 32 27
HincII GTy'rAC
Cuts at: 0 876 990
Size: 876 114
KasI G'GCGC_C
Cuts at: 0 717 990
Size: 717 273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 990
Size: 170 820
MscI TGG'CCA
Cuts at: 0 187 990
Size: 187 803
MslI CAynn'nnrTG
Cuts at: 0 211 709 979 990
Size: 211 498 270 11
Fragments arranged by size:
498 270 211 11
MspA1I CmG'CkG
Cuts at: 0 503 527 554 608 959 990
Size: 503 24 27 54 351 31
Fragments arranged by size:
503 351 54 31 27 24
NarI GG'CG_CC
Cuts at: 0 718 990
Size: 718 272
NcoI C'CATG_G
Cuts at: 0 74 990
Size: 74 916
NspI r_CATG'y
Cuts at: 0 402 454 477 684 990
Size: 402 52 23 207 306
Fragments arranged by size:
402 306 207 52 23
PasI CC'CwG_GG
Cuts at: 0 732 885 894 990
Size: 732 153 9 96
Fragments arranged by size:
732 153 96 9
PfoI T'CCnGG_A
Cuts at: 0 767 848 990
Size: 767 81 142
Fragments arranged by size:
767 142 81
PpuMI rG'GwC_Cy
Cuts at: 0 458 840 951 990
Size: 458 382 111 39
Fragments arranged by size:
458 382 111 39
PstI C_TGCA'G
Cuts at: 0 482 802 990
Size: 482 320 188
Fragments arranged by size:
482 320 188
PvuII CAG'CTG
Cuts at: 0 554 959 990
Size: 554 405 31
Fragments arranged by size:
554 405 31
SalI G'TCGA_C
Cuts at: 0 874 990
Size: 874 116
ScaI AGT'ACT
Cuts at: 0 442 990
Size: 442 548
SexAI A'CCwGG_T
Cuts at: 0 296 990
Size: 296 694
SfcI C'TryA_G
Cuts at: 0 478 798 990
Size: 478 320 192
Fragments arranged by size:
478 320 192
SfoI GGC'GCC
Cuts at: 0 719 990
Size: 719 271
SphI G_CATG'C
Cuts at: 0 402 454 990
Size: 402 52 536
Fragments arranged by size:
536 402 52
StyI C'CwwG_G
Cuts at: 0 74 188 347 990
Size: 74 114 159 643
Fragments arranged by size:
643 159 114 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 990
Size: 118 872
TatI w'GTAC_w
Cuts at: 0 248 440 683 990
Size: 248 192 243 307
Fragments arranged by size:
307 248 243 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 354 711 729 990
Size: 180 174 357 18 261
Fragments arranged by size:
357 261 180 174 18
ZraI GAC'GTC
Cuts at: 0 168 990
Size: 168 822
Enzymes that do cut:
AarI AatII AccI AcuI ApoI BanI BanII BbeI
BbsI BlpI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NcoI NspI PasI PfoI PpuMI
PstI PvuII SalI ScaI SexAI SfcI SfoI SphI
StyI TaqII TatI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BseRI BsiWI BsmI BspEI BsrBI
BsrFI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI