Restriction map for
pF1KSDA0283
(Linear) (Six-base) MAPSORT of: KIAA0283.seq Check: 6611 from: 1 to: 4323
KIAA0283.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2208 4323
Size: 2208 2115
AatII G_ACGT'C
Cuts at: 0 2480 4323
Size: 2480 1843
AccI GT'mk_AC
Cuts at: 0 365 1019 1187 3020 4289 4323
Size: 365 654 168 1833 1269 34
Fragments arranged by size:
1833 1269 654 365 168 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 985 1882 4323
Size: 304 681 897 2441
Fragments arranged by size:
2441 897 681 304
AfeI AGC'GCT
Cuts at: 0 4051 4323
Size: 4051 272
AflIII A'CryG_T
Cuts at: 0 4214 4323
Size: 4214 109
AhdI GACnn_n'nnGTC
Cuts at: 0 2882 4323
Size: 2882 1441
AleI CACnn'nnGTG
Cuts at: 0 721 3251 4323
Size: 721 2530 1072
Fragments arranged by size:
2530 1072 721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2888 4323
Size: 2888 1435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2749 2781 4323
Size: 2749 32 1542
Fragments arranged by size:
2749 1542 32
AlwNI CAG_nnn'CTG
Cuts at: 0 119 769 1459 4323
Size: 119 650 690 2864
Fragments arranged by size:
2864 690 650 119
ApaI G_GGCC'C
Cuts at: 0 1490 1727 3236 3363 3893 4323
Size: 1490 237 1509 127 530 430
Fragments arranged by size:
1509 1490 530 430 237 127
ApaLI G'TGCA_C
Cuts at: 0 3406 4323
Size: 3406 917
ApoI r'AATT_y
Cuts at: 0 1754 2190 3532 4282 4323
Size: 1754 436 1342 750 41
Fragments arranged by size:
1754 1342 750 436 41
AvaI C'yCGr_G
Cuts at: 0 68 1399 1605 1847 2582 2780 3571 4323
Size: 68 1331 206 242 735 198 791 752
Fragments arranged by size:
1331 791 752 735 242 206 198 68
BanI G'GyrC_C
Cuts at: 0 64 1941 2176 4323
Size: 64 1877 235 2147
Fragments arranged by size:
2147 1877 235 64
BanII G_rGCy'C
Cuts at: 0 75 452 1200 1226 1490 1727 1857 2382
Size: 75 377 748 26 264 237 130 525
Cuts at: 2382 3059 3125 3236 3356 3363 3593 3893 4323
Size: 677 66 111 120 7 230 300 430
Fragments arranged by size:
748 677 525 430 377 300 264 237
230 130 120 111 75 66 26 7
BbeI G_GCGC'C
Cuts at: 0 68 4323
Size: 68 4255
BbsI GAAGACnn'nnnn_
Cuts at: 0 1451 3877 4323
Size: 1451 2426 446
Fragments arranged by size:
2426 1451 446
BbvCI CC'TCA_GC
Cuts at: 0 25 4323
Size: 25 4298
BciVI GTATCCnnnnn_n'
Cuts at: 0 2842 4323
Size: 2842 1481
BglI GCCn_nnn'nGGC
Cuts at: 0 3188 3437 4323
Size: 3188 249 886
Fragments arranged by size:
3188 886 249
BglII A'GATC_T
Cuts at: 0 1502 4323
Size: 1502 2821
BlpI GC'TnA_GC
Cuts at: 0 2431 4323
Size: 2431 1892
Bme1580I G_kGCm'C
Cuts at: 0 209 1490 1727 1946 3236 3363 3410 3893
Size: 209 1281 237 219 1290 127 47 483
Cuts at: 3893 4323
Size: 430
Fragments arranged by size:
1290 1281 483 430 237 219 209 127
47
BmgBI CAC'GTC
Cuts at: 0 3630 4215 4323
Size: 3630 585 108
Fragments arranged by size:
3630 585 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 3837 4323
Size: 3837 486
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1099 3891 3927 4323
Size: 1099 2792 36 396
Fragments arranged by size:
2792 1099 396 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 664 1950 3498 3801 3988 4099 4323
Size: 312 352 1286 1548 303 187 111 224
Fragments arranged by size:
1548 1286 352 312 303 224 187 111
Bpu10I CC'TnA_GC
Cuts at: 0 25 4323
Size: 25 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2864 4323
Size: 2864 1459
BsaI GGTCTCn'nnnn_
Cuts at: 0 662 1000 1525 2134 2359 2801 4258 4323
Size: 662 338 525 609 225 442 1457 65
Fragments arranged by size:
1457 662 609 525 442 338 225 65
BsaAI yAC'GTr
Cuts at: 0 369 998 4323
Size: 369 629 3325
Fragments arranged by size:
3325 629 369
BsaBI GATnn'nnATC
Cuts at: 0 1507 3614 3983 4323
Size: 1507 2107 369 340
Fragments arranged by size:
2107 1507 369 340
BsaHI Gr'CG_yC
Cuts at: 0 65 2477 4323
Size: 65 2412 1846
Fragments arranged by size:
2412 1846 65
BsaWI w'CCGG_w
Cuts at: 0 1155 1634 4323
Size: 1155 479 2689
Fragments arranged by size:
2689 1155 479
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 1844 1874 3790 3820 4323
Size: 310 30 1504 30 1916 30 503
Fragments arranged by size:
1916 1504 503 310 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1072 1320 1327 1435 1510 1903 2069 2251
Size: 1072 248 7 108 75 393 166 182
Cuts at: 2251 3403 3798 4323
Size: 1152 395 525
Fragments arranged by size:
1152 1072 525 395 393 248 182 166
108 75 7
BseYI C'CCAG_C
Cuts at: 0 434 773 2027 2516 3229 3256 3262 3746
Size: 434 339 1254 489 713 27 6 484
Cuts at: 3746 4323
Size: 577
Fragments arranged by size:
1254 713 577 489 484 434 339 27
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1291 1453 4323
Size: 1169 122 162 2870
Fragments arranged by size:
2870 1169 162 122
BsiHKAI G_wGCw'C
Cuts at: 0 110 452 1082 1857 3125 3356 3410 4323
Size: 110 342 630 775 1268 231 54 913
Fragments arranged by size:
1268 913 775 630 342 231 110 54
BsmI GAATG_Cn'
Cuts at: 0 627 1965 4323
Size: 627 1338 2358
Fragments arranged by size:
2358 1338 627
BsmBI CGTCTCn'nnnn_
Cuts at: 0 507 3915 4323
Size: 507 3408 408
Fragments arranged by size:
3408 507 408
Bsp1286I G_dGCh'C
Cuts at: 0 75 110 209 452 1082 1200 1226 1490
Size: 75 35 99 243 630 118 26 264
Cuts at: 1490 1727 1857 1946 2382 3059 3125 3236 3356
Size: 237 130 89 436 677 66 111 120
Cuts at: 3356 3363 3410 3593 3893 4323
Size: 7 47 183 300 430
Fragments arranged by size:
677 630 436 430 300 264 243 237
183 130 120 118 111 99 89 75
66 47 35 26 7
BspEI T'CCGG_A
Cuts at: 0 1634 4323
Size: 1634 2689
BspHI T'CATG_A
Cuts at: 0 221 1364 4323
Size: 221 1143 2959
Fragments arranged by size:
2959 1143 221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2024 2208 4323
Size: 2024 184 2115
Fragments arranged by size:
2115 2024 184
BsrBI CCG'CTC
Cuts at: 0 809 1840 4323
Size: 809 1031 2483
Fragments arranged by size:
2483 1031 809
BsrDI GCAATG_nn'
Cuts at: 0 638 691 872 2451 2870 3281 4323
Size: 638 53 181 1579 419 411 1042
Fragments arranged by size:
1579 1042 638 419 411 181 53
BstAPI GCAn_nnn'nTGC
Cuts at: 0 769 4323
Size: 769 3554
BstEII G'GTnAC_C
Cuts at: 0 714 1239 3741 4323
Size: 714 525 2502 582
Fragments arranged by size:
2502 714 582 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2610 3768 3900 4012 4260 4323
Size: 2610 1158 132 112 248 63
Fragments arranged by size:
2610 1158 248 132 112 63
BstYI r'GATC_y
Cuts at: 0 214 1070 1152 1502 3113 4323
Size: 214 856 82 350 1611 1210
Fragments arranged by size:
1611 1210 856 350 214 82
BstZ17I GTA'TAC
Cuts at: 0 4290 4323
Size: 4290 33
Bsu36I CC'TnA_GG
Cuts at: 0 37 4323
Size: 37 4286
BtgI C'CryG_G
Cuts at: 0 533 2502 3156 3767 4323
Size: 533 1969 654 611 556
Fragments arranged by size:
1969 654 611 556 533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 513 4323
Size: 513 3810
BtsI GCAGTG_nn'
Cuts at: 0 55 209 300 767 1222 3246 3258 3443
Size: 55 154 91 467 455 2024 12 185
Cuts at: 3443 3836 4128 4182 4234 4323
Size: 393 292 54 52 89
Fragments arranged by size:
2024 467 455 393 292 185 154 91
89 55 54 52 12
ClaI AT'CG_AT
Cuts at: 0 2748 4323
Size: 2748 1575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 855 890 1725 1760 2948 2983 4323
Size: 855 35 835 35 1188 35 1340
Fragments arranged by size:
1340 1188 855 835 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 3562 4227 4323
Size: 3562 665 96
Fragments arranged by size:
3562 665 96
DrdI GACnn_nn'nnGTC
Cuts at: 0 3016 3034 4323
Size: 3016 18 1289
Fragments arranged by size:
3016 1289 18
EaeI y'GGCC_r
Cuts at: 0 252 469 2348 3001 3308 3970 4323
Size: 252 217 1879 653 307 662 353
Fragments arranged by size:
1879 662 653 353 307 252 217
EarI CTCTTCn'nnn_
Cuts at: 0 2466 2638 3136 4323
Size: 2466 172 498 1187
Fragments arranged by size:
2466 1187 498 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2928 3115 3187 3282 4323
Size: 2928 187 72 95 1041
Fragments arranged by size:
2928 1041 187 95 72
EcoICRI GAG'CTC
Cuts at: 0 450 1855 3123 3354 4323
Size: 450 1405 1268 231 969
Fragments arranged by size:
1405 1268 969 450 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 312 664 985 1882 1950 3498 3801
Size: 304 8 352 321 897 68 1548 303
Cuts at: 3801 3988 4099 4323
Size: 187 111 224
Fragments arranged by size:
1548 897 352 321 304 303 224 187
111 68 8
EcoO109I rG'GnC_Cy
Cuts at: 0 353 549 1120 1486 1487 1724 2395 4323
Size: 353 196 571 366 1 237 671 1928
Fragments arranged by size:
1928 671 571 366 353 237 196 1
EcoRI G'AATT_C
Cuts at: 0 2190 4323
Size: 2190 2133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2336 4323
Size: 2336 1987
HaeII r_GCGC'y
Cuts at: 0 68 83 741 2438 4053 4323
Size: 68 15 658 1697 1615 270
Fragments arranged by size:
1697 1615 658 270 68 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 805 837 1844 1876 2523 2555 2800 2832
Size: 805 32 1007 32 647 32 245 32
Cuts at: 2832 2868 2900 3664 3696 4323
Size: 36 32 764 32 627
Fragments arranged by size:
1007 805 764 647 627 245 36 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 729 1020 2481 3372 4323
Size: 729 291 1461 891 951
Fragments arranged by size:
1461 951 891 729 291
HindIII A'AGCT_T
Cuts at: 0 670 4323
Size: 670 3653
KasI G'GCGC_C
Cuts at: 0 64 4323
Size: 64 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 2619 4095 4277 4323
Size: 857 1762 1476 182 46
Fragments arranged by size:
1762 1476 857 182 46
MscI TGG'CCA
Cuts at: 0 254 471 2350 3003 4323
Size: 254 217 1879 653 1320
Fragments arranged by size:
1879 1320 653 254 217
MslI CAynn'nnrTG
Cuts at: 0 226 532 633 721 912 1822 1825 2608
Size: 226 306 101 88 191 910 3 783
Cuts at: 2608 2638 2950 3251 3454 3898 4213 4323
Size: 30 312 301 203 444 315 110
Fragments arranged by size:
910 783 444 315 312 306 301 226
203 191 110 101 88 30 3
MspA1I CmG'CkG
Cuts at: 0 48 777 797 1206 1247 1875 1918 2504
Size: 48 729 20 409 41 628 43 586
Cuts at: 2504 3146 3647 4323
Size: 642 501 676
Fragments arranged by size:
729 676 642 628 586 501 409 48
43 41 20
NarI GG'CG_CC
Cuts at: 0 65 4323
Size: 65 4258
PasI CC'CwG_GG
Cuts at: 0 349 1672 4323
Size: 349 1323 2651
Fragments arranged by size:
2651 1323 349
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 3979 4323
Size: 600 3379 344
Fragments arranged by size:
3379 600 344
PfoI T'CCnGG_A
Cuts at: 0 1452 3116 3419 4323
Size: 1452 1664 303 904
Fragments arranged by size:
1664 1452 904 303
PmlI CAC'GTG
Cuts at: 0 998 4323
Size: 998 3325
PpuMI rG'GwC_Cy
Cuts at: 0 549 4323
Size: 549 3774
PspOMI G'GGCC_C
Cuts at: 0 1486 1723 3232 3359 3889 4323
Size: 1486 237 1509 127 530 434
Fragments arranged by size:
1509 1486 530 434 237 127
PstI C_TGCA'G
Cuts at: 0 47 1217 2627 3253 3583 4177 4323
Size: 47 1170 1410 626 330 594 146
Fragments arranged by size:
1410 1170 626 594 330 146 47
PvuII CAG'CTG
Cuts at: 0 48 1206 1875 1918 3146 4323
Size: 48 1158 669 43 1228 1177
Fragments arranged by size:
1228 1177 1158 669 48 43
SacI G_AGCT'C
Cuts at: 0 452 1857 3125 3356 4323
Size: 452 1405 1268 231 967
Fragments arranged by size:
1405 1268 967 452 231
SacII CC_GC'GG
Cuts at: 0 2505 4323
Size: 2505 1818
SalI G'TCGA_C
Cuts at: 0 1018 4323
Size: 1018 3305
SexAI A'CCwGG_T
Cuts at: 0 1092 2963 3374 4323
Size: 1092 1871 411 949
Fragments arranged by size:
1871 1092 949 411
SfcI C'TryA_G
Cuts at: 0 43 111 1213 2100 2623 3249 3579 4173
Size: 43 68 1102 887 523 626 330 594
Cuts at: 4173 4323
Size: 150
Fragments arranged by size:
1102 887 626 594 523 330 150 68
43
SfoI GGC'GCC
Cuts at: 0 66 4323
Size: 66 4257
SmaI CCC'GGG
Cuts at: 0 70 1849 2584 2782 4323
Size: 70 1779 735 198 1541
Fragments arranged by size:
1779 1541 735 198 70
SmlI C'TyrA_G
Cuts at: 0 1605 2879 4323
Size: 1605 1274 1444
Fragments arranged by size:
1605 1444 1274
SrfI GCCC'GGGC
Cuts at: 0 70 4323
Size: 70 4253
SspI AAT'ATT
Cuts at: 0 3947 4309 4323
Size: 3947 362 14
Fragments arranged by size:
3947 362 14
StuI AGG'CCT
Cuts at: 0 1352 2683 4323
Size: 1352 1331 1640
Fragments arranged by size:
1640 1352 1331
StyI C'CwwG_G
Cuts at: 0 390 1479 2720 4323
Size: 390 1089 1241 1603
Fragments arranged by size:
1603 1241 1089 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 426 2032 2041 2200 3760 4323
Size: 313 113 1606 9 159 1560 563
Fragments arranged by size:
1606 1560 563 313 159 113 9
TatI w'GTAC_w
Cuts at: 0 4007 4323
Size: 4007 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1131 1163 4106 4138 4323
Size: 1131 32 2943 32 185
Fragments arranged by size:
2943 1131 185 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 541 4323
Size: 541 3782
XbaI T'CTAG_A
Cuts at: 0 1467 4323
Size: 1467 2856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 688 756 2949 3183 3253 3303 4135 4323
Size: 688 68 2193 234 70 50 832 188
Fragments arranged by size:
2193 832 688 234 188 70 68 50
XhoI C'TCGA_G
Cuts at: 0 1605 4323
Size: 1605 2718
XmaI C'CCGG_G
Cuts at: 0 68 1847 2582 2780 4323
Size: 68 1779 735 198 1543
Fragments arranged by size:
1779 1543 735 198 68
XmnI GAAnn'nnTTC
Cuts at: 0 1448 1624 4323
Size: 1448 176 2699
Fragments arranged by size:
2699 1448 176
ZraI GAC'GTC
Cuts at: 0 2478 4323
Size: 2478 1845
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NarI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SacII SalI SexAI SfcI SfoI SmaI SmlI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BclI BfrBI BmtI BsiEI BsiWI
BsrFI BsrGI BssHII BssSI BstBI DraI EagI EcoNI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SwaI