Restriction map for
pF1KA0284
(Linear) (Six-base) MAPSORT of: KIAA0284.seq Check: 9035 from: 1 to: 4557
KIAA0284.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 138 541 927 4557
Size: 138 403 386 3630
Fragments arranged by size:
3630 403 386 138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 856 3176 3474 4264 4297 4557
Size: 52 804 2320 298 790 33 260
Fragments arranged by size:
2320 804 790 298 260 52 33
AfeI AGC'GCT
Cuts at: 0 1042 4557
Size: 1042 3515
AflIII A'CryG_T
Cuts at: 0 71 3560 4557
Size: 71 3489 997
Fragments arranged by size:
3489 997 71
AhdI GACnn_n'nnGTC
Cuts at: 0 2637 4557
Size: 2637 1920
AleI CACnn'nnGTG
Cuts at: 0 1528 4557
Size: 1528 3029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1706 4557
Size: 1706 2851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4093 4125 4557
Size: 4093 32 432
Fragments arranged by size:
4093 432 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1311 2472 2724 3199 3262 3358 3786 4557
Size: 1311 1161 252 475 63 96 428 771
Fragments arranged by size:
1311 1161 771 475 428 252 96 63
ApaI G_GGCC'C
Cuts at: 0 1408 1933 2120 2154 2702 3177 3501 3684
Size: 1408 525 187 34 548 475 324 183
Cuts at: 3684 4557
Size: 873
Fragments arranged by size:
1408 873 548 525 475 324 187 183
34
ApoI r'AATT_y
Cuts at: 0 4476 4557
Size: 4476 81
AvaI C'yCGr_G
Cuts at: 0 508 681 756 1164 1345 1812 2065 2155
Size: 508 173 75 408 181 467 253 90
Cuts at: 2155 2483 2616 2772 2900 3062 3346 3504 3645
Size: 328 133 156 128 162 284 158 141
Cuts at: 3645 3677 3692 3923 3949 3998 4557
Size: 32 15 231 26 49 559
Fragments arranged by size:
559 508 467 408 328 284 253 231
181 173 162 158 156 141 133 128
90 75 49 32 26 15
AvrII C'CTAG_G
Cuts at: 0 2253 4557
Size: 2253 2304
BamHI G'GATC_C
Cuts at: 0 2016 4557
Size: 2016 2541
BanI G'GyrC_C
Cuts at: 0 168 513 912 1271 1743 2779 3536 3975
Size: 168 345 399 359 472 1036 757 439
Cuts at: 3975 4233 4557
Size: 258 324
Fragments arranged by size:
1036 757 472 439 399 359 345 324
258 168
BanII G_rGCy'C
Cuts at: 0 359 404 458 1324 1408 1753 1933 2107
Size: 359 45 54 866 84 345 180 174
Cuts at: 2107 2120 2154 2702 3177 3245 3501 3553 3672
Size: 13 34 548 475 68 256 52 119
Cuts at: 3672 3684 3856 4426 4557
Size: 12 172 570 131
Fragments arranged by size:
866 570 548 475 359 345 256 180
174 172 131 119 84 68 54 52
45 34 13 12
BbeI G_GCGC'C
Cuts at: 0 172 1275 3540 4557
Size: 172 1103 2265 1017
Fragments arranged by size:
2265 1103 1017 172
BbsI GAAGACnn'nnnn_
Cuts at: 0 1207 4252 4557
Size: 1207 3045 305
Fragments arranged by size:
3045 1207 305
BbvCI CC'TCA_GC
Cuts at: 0 1895 2029 3166 3883 4440 4557
Size: 1895 134 1137 717 557 117
Fragments arranged by size:
1895 1137 717 557 134 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 747 781 2347 2381 4557
Size: 747 34 1566 34 2176
Fragments arranged by size:
2176 1566 747 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 895 1403 1720 1792 1928 2000 2554 2563
Size: 895 508 317 72 136 72 554 9
Cuts at: 2563 2868 3004 4557
Size: 305 136 1553
Fragments arranged by size:
1553 895 554 508 317 305 136 136
72 72 9
BlpI GC'TnA_GC
Cuts at: 0 425 2874 3084 3263 3775 4557
Size: 425 2449 210 179 512 782
Fragments arranged by size:
2449 782 512 425 210 179
Bme1580I G_kGCm'C
Cuts at: 0 917 1408 1933 2120 2154 2702 2733 2799
Size: 917 491 525 187 34 548 31 66
Cuts at: 2799 3177 3501 3516 3684 4238 4557
Size: 378 324 15 168 554 319
Fragments arranged by size:
917 554 548 525 491 378 324 319
187 168 66 34 31 15
BmgBI CAC'GTC
Cuts at: 0 2655 3563 3798 4557
Size: 2655 908 235 759
Fragments arranged by size:
2655 908 759 235
BmrI ACTGGGnnnn_n'
Cuts at: 0 1041 1121 1135 1299 1612 1714 1898 2915
Size: 1041 80 14 164 313 102 184 1017
Cuts at: 2915 3859 4328 4557
Size: 944 469 229
Fragments arranged by size:
1041 1017 944 469 313 229 184 164
102 80 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 285 1710 2826 3603 4557
Size: 285 1425 1116 777 954
Fragments arranged by size:
1425 1116 954 777 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1575 2219 2433 2581 3426 3645 4557
Size: 103 1472 644 214 148 845 219 912
Fragments arranged by size:
1472 912 845 644 219 214 148 103
Bpu10I CC'TnA_GC
Cuts at: 0 1895 2029 3166 3239 3883 4440 4557
Size: 1895 134 1137 73 644 557 117
Fragments arranged by size:
1895 1137 644 557 134 117 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1174 4557
Size: 103 1071 3383
Fragments arranged by size:
3383 1071 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 243 478 715 1501 4557
Size: 243 235 237 786 3056
Fragments arranged by size:
3056 786 243 237 235
BsaAI yAC'GTr
Cuts at: 0 2123 4557
Size: 2123 2434
BsaBI GATnn'nnATC
Cuts at: 0 634 4165 4557
Size: 634 3531 392
Fragments arranged by size:
3531 634 392
BsaHI Gr'CG_yC
Cuts at: 0 169 338 1272 2294 2633 3537 4037 4557
Size: 169 169 934 1022 339 904 500 520
Fragments arranged by size:
1022 934 904 520 500 339 169 169
BsaWI w'CCGG_w
Cuts at: 0 245 1359 1965 4557
Size: 245 1114 606 2592
Fragments arranged by size:
2592 1114 606 245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2118 2148 2491 2521 2849 2879 3601 3631
Size: 2118 30 343 30 328 30 722 30
Cuts at: 3631 3862 3892 4557
Size: 231 30 665
Fragments arranged by size:
2118 722 665 343 328 231 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 192 1480 1549 1962 2023 2563 2941
Size: 124 68 1288 69 413 61 540 378
Cuts at: 2941 3082 3589 3592 3727 3730 3846 3943 4087
Size: 141 507 3 135 3 116 97 144
Cuts at: 4087 4557
Size: 470
Fragments arranged by size:
1288 540 507 470 413 378 144 141
135 124 116 97 69 68 61 3
3
BseYI C'CCAG_C
Cuts at: 0 341 457 907 1134 1215 1339 1439 1664
Size: 341 116 450 227 81 124 100 225
Cuts at: 1664 1781 1987 2267 2272 2280 2370 2421 2701
Size: 117 206 280 5 8 90 51 280
Cuts at: 2701 2717 2956 3054 3180 3832 3895 4112 4395
Size: 16 239 98 126 652 63 217 283
Cuts at: 4395 4507 4557
Size: 112 50
Fragments arranged by size:
652 450 341 283 280 280 239 227
225 217 206 126 124 117 116 112
100 98 90 81 63 51 50 16
8 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 406 598 751 766 2747 3763 3958
Size: 112 294 192 153 15 1981 1016 195
Cuts at: 3958 4315 4367 4557
Size: 357 52 190
Fragments arranged by size:
1981 1016 357 294 195 192 190 153
112 52 15
BsiEI CG_ry'CG
Cuts at: 0 905 1112 1331 4557
Size: 905 207 219 3226
Fragments arranged by size:
3226 905 219 207
BsiHKAI G_wGCw'C
Cuts at: 0 404 1324 1730 4148 4557
Size: 404 920 406 2418 409
Fragments arranged by size:
2418 920 409 406 404
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2530 2536 4557
Size: 2530 6 2021
Fragments arranged by size:
2530 2021 6
Bsp1286I G_dGCh'C
Cuts at: 0 359 404 458 917 1324 1408 1730 1753
Size: 359 45 54 459 407 84 322 23
Cuts at: 1753 1933 2107 2120 2154 2702 2733 2799 3177
Size: 180 174 13 34 548 31 66 378
Cuts at: 3177 3245 3501 3516 3553 3672 3684 3856 4148
Size: 68 256 15 37 119 12 172 292
Cuts at: 4148 4238 4426 4557
Size: 90 188 131
Fragments arranged by size:
548 459 407 378 359 322 292 256
188 180 174 172 131 119 90 84
68 66 54 45 37 34 31 23
15 13 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 541 821 927 4358 4557
Size: 138 403 280 106 3431 199
Fragments arranged by size:
3431 403 280 199 138 106
BsrBI CCG'CTC
Cuts at: 0 565 1277 1372 2696 3122 3219 3503 4557
Size: 565 712 95 1324 426 97 284 1054
Fragments arranged by size:
1324 1054 712 565 426 284 97 95
BsrFI r'CCGG_y
Cuts at: 0 516 1107 1180 2862 3251 3319 3539 4557
Size: 516 591 73 1682 389 68 220 1018
Fragments arranged by size:
1682 1018 591 516 389 220 73 68
BssSI C'ACGA_G
Cuts at: 0 523 850 3531 4557
Size: 523 327 2681 1026
Fragments arranged by size:
2681 1026 523 327
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3358 3757 4557
Size: 3358 399 800
Fragments arranged by size:
3358 800 399
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1401 1564 4557
Size: 1401 163 2993
Fragments arranged by size:
2993 1401 163
BstYI r'GATC_y
Cuts at: 0 470 2016 3818 4166 4557
Size: 470 1546 1802 348 391
Fragments arranged by size:
1802 1546 470 391 348
Bsu36I CC'TnA_GG
Cuts at: 0 431 2993 4471 4557
Size: 431 2562 1478 86
Fragments arranged by size:
2562 1478 431 86
BtgI C'CryG_G
Cuts at: 0 405 1095 1386 1400 1430 2853 3245 3419
Size: 405 690 291 14 30 1423 392 174
Cuts at: 3419 3470 3594 4557
Size: 51 124 963
Fragments arranged by size:
1423 963 690 405 392 291 174 124
51 30 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2048 4557
Size: 2048 2509
BtsI GCAGTG_nn'
Cuts at: 0 187 3390 3516 4557
Size: 187 3203 126 1041
Fragments arranged by size:
3203 1041 187 126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1077 1112 4557
Size: 1077 35 3445
Fragments arranged by size:
3445 1077 35
DraIII CAC_nnn'GTG
Cuts at: 0 1708 3828 4557
Size: 1708 2120 729
Fragments arranged by size:
2120 1708 729
DrdI GACnn_nn'nnGTC
Cuts at: 0 817 4557
Size: 817 3740
EaeI y'GGCC_r
Cuts at: 0 731 887 902 1109 1328 1604 1699 2001
Size: 731 156 15 207 219 276 95 302
Cuts at: 2001 2474 2889 3253 3299 3378 3422 3800 4043
Size: 473 415 364 46 79 44 378 243
Cuts at: 4043 4373 4557
Size: 330 184
Fragments arranged by size:
731 473 415 378 364 330 302 276
243 219 207 184 156 95 79 46
44 15
EagI C'GGCC_G
Cuts at: 0 902 1109 1328 4557
Size: 902 207 219 3229
Fragments arranged by size:
3229 902 219 207
EarI CTCTTCn'nnn_
Cuts at: 0 2002 4557
Size: 2002 2555
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 575 1738 4557
Size: 575 1163 2819
Fragments arranged by size:
2819 1163 575
EcoICRI GAG'CTC
Cuts at: 0 402 1322 4557
Size: 402 920 3235
Fragments arranged by size:
3235 920 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 103 856 1575 2219 2433 2581 3176
Size: 52 51 753 719 644 214 148 595
Cuts at: 3176 3426 3474 3645 4264 4297 4557
Size: 250 48 171 619 33 260
Fragments arranged by size:
753 719 644 619 595 260 250 214
171 148 52 51 48 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 1288 1822 2814 3492 3811 4557
Size: 889 399 534 992 678 319 746
Fragments arranged by size:
992 889 746 678 534 399 319
EcoO109I rG'GnC_Cy
Cuts at: 0 379 879 966 1074 1405 1734 1862 2062
Size: 379 500 87 108 331 329 128 200
Cuts at: 2062 2116 2150 2151 2250 2469 2769 2897 2906
Size: 54 34 1 99 219 300 128 9
Cuts at: 2906 3224 3475 3497 3681 4200 4449 4557
Size: 318 251 22 184 519 249 108
Fragments arranged by size:
519 500 379 331 329 318 300 251
249 219 200 184 128 128 108 108
99 87 54 34 22 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4188 4557
Size: 4188 369
FseI GG_CCGG'CC
Cuts at: 0 3255 4557
Size: 3255 1302
FspI TGC'GCA
Cuts at: 0 4 1876 4557
Size: 4 1872 2681
Fragments arranged by size:
2681 1872 4
FspAI rTGC'GCAy
Cuts at: 0 4 1876 4557
Size: 4 1872 2681
Fragments arranged by size:
2681 1872 4
HaeII r_GCGC'y
Cuts at: 0 172 348 372 1044 1095 1275 1678 2142
Size: 172 176 24 672 51 180 403 464
Cuts at: 2142 2428 2874 3540 3878 4557
Size: 286 446 666 338 679
Fragments arranged by size:
679 672 666 464 446 403 338 286
180 176 172 51 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 697 729 1789 1821 2118 2150 2491 2523
Size: 697 32 1060 32 297 32 341 32
Cuts at: 2523 2623 2655 2808 2840 3208 3240 3547 3579
Size: 100 32 153 32 368 32 307 32
Cuts at: 3579 4044 4076 4557
Size: 465 32 481
Fragments arranged by size:
1060 697 481 465 368 341 307 297
153 100 32 32 32 32 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 168 1271 3536 4557
Size: 168 1103 2265 1021
Fragments arranged by size:
2265 1103 1021 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1553 1994 2027 3002 3149 3551 4557
Size: 92 1461 441 33 975 147 402 1006
Fragments arranged by size:
1461 1006 975 441 402 147 92 33
MscI TGG'CCA
Cuts at: 0 889 2891 3301 3802 4045 4375 4557
Size: 889 2002 410 501 243 330 182
Fragments arranged by size:
2002 889 501 410 330 243 182
MslI CAynn'nnrTG
Cuts at: 0 76 1528 4557
Size: 76 1452 3029
Fragments arranged by size:
3029 1452 76
MspA1I CmG'CkG
Cuts at: 0 144 672 907 929 1443 1668 1781 1899
Size: 144 528 235 22 514 225 113 118
Cuts at: 1899 2761 3037 3058 3259 4028 4116 4305 4488
Size: 862 276 21 201 769 88 189 183
Cuts at: 4488 4496 4557
Size: 8 61
Fragments arranged by size:
862 769 528 514 276 235 225 201
189 183 144 118 113 88 61 22
21 8
NaeI GCC'GGC
Cuts at: 0 518 1109 1182 3253 3321 3541 4557
Size: 518 591 73 2071 68 220 1016
Fragments arranged by size:
2071 1016 591 518 220 73 68
NarI GG'CG_CC
Cuts at: 0 169 1272 3537 4557
Size: 169 1103 2265 1020
Fragments arranged by size:
2265 1103 1020 169
NcoI C'CATG_G
Cuts at: 0 1386 3419 4557
Size: 1386 2033 1138
Fragments arranged by size:
2033 1386 1138
NgoMIV G'CCGG_C
Cuts at: 0 516 1107 1180 3251 3319 3539 4557
Size: 516 591 73 2071 68 220 1018
Fragments arranged by size:
2071 1018 591 516 220 73 68
NspI r_CATG'y
Cuts at: 0 75 1881 3894 4557
Size: 75 1806 2013 663
Fragments arranged by size:
2013 1806 663 75
PasI CC'CwG_GG
Cuts at: 0 781 1756 3228 4557
Size: 781 975 1472 1329
Fragments arranged by size:
1472 1329 975 781
PciI A'CATG_T
Cuts at: 0 71 4557
Size: 71 4486
PflMI CCAn_nnn'nTGG
Cuts at: 0 1671 2242 2413 3786 3828 4557
Size: 1671 571 171 1373 42 729
Fragments arranged by size:
1671 1373 729 571 171 42
PfoI T'CCnGG_A
Cuts at: 0 8 104 3442 4514 4557
Size: 8 96 3338 1072 43
Fragments arranged by size:
3338 1072 96 43 8
PmlI CAC'GTG
Cuts at: 0 2123 4557
Size: 2123 2434
PpuMI rG'GwC_Cy
Cuts at: 0 379 879 966 1074 1862 2469 2769 2906
Size: 379 500 87 108 788 607 300 137
Cuts at: 2906 3224 3475 4200 4557
Size: 318 251 725 357
Fragments arranged by size:
788 725 607 500 379 357 318 300
251 137 108 87
PspOMI G'GGCC_C
Cuts at: 0 1404 1929 2116 2150 2698 3173 3497 3680
Size: 1404 525 187 34 548 475 324 183
Cuts at: 3680 4557
Size: 877
Fragments arranged by size:
1404 877 548 525 475 324 187 183
34
PstI C_TGCA'G
Cuts at: 0 149 619 950 1685 4557
Size: 149 470 331 735 2872
Fragments arranged by size:
2872 735 470 331 149
PvuII CAG'CTG
Cuts at: 0 144 1443 1899 2761 3058 3259 4028 4116
Size: 144 1299 456 862 297 201 769 88
Cuts at: 4116 4305 4496 4557
Size: 189 191 61
Fragments arranged by size:
1299 862 769 456 297 201 191 189
144 88 61
SacI G_AGCT'C
Cuts at: 0 404 1324 4557
Size: 404 920 3233
Fragments arranged by size:
3233 920 404
SanDI GG'GwC_CC
Cuts at: 0 379 966 1074 1862 2906 3224 3475 4557
Size: 379 587 108 788 1044 318 251 1082
Fragments arranged by size:
1082 1044 788 587 379 318 251 108
SexAI A'CCwGG_T
Cuts at: 0 1556 4557
Size: 1556 3001
SfcI C'TryA_G
Cuts at: 0 145 615 946 1681 2464 4557
Size: 145 470 331 735 783 2093
Fragments arranged by size:
2093 783 735 470 331 145
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 895 2554 2563 4557
Size: 895 1659 9 1994
Fragments arranged by size:
1994 1659 895 9
SfoI GGC'GCC
Cuts at: 0 170 1273 3538 4557
Size: 170 1103 2265 1019
Fragments arranged by size:
2265 1103 1019 170
SmaI CCC'GGG
Cuts at: 0 683 1814 2157 2485 2774 3064 3679 3694
Size: 683 1131 343 328 289 290 615 15
Cuts at: 3694 3925 4557
Size: 231 632
Fragments arranged by size:
1131 683 632 615 343 328 290 289
231 15
SmlI C'TyrA_G
Cuts at: 0 118 1189 4557
Size: 118 1071 3368
Fragments arranged by size:
3368 1071 118
SpeI A'CTAG_T
Cuts at: 0 3337 4557
Size: 3337 1220
SphI G_CATG'C
Cuts at: 0 1881 4557
Size: 1881 2676
StuI AGG'CCT
Cuts at: 0 217 997 3112 4557
Size: 217 780 2115 1445
Fragments arranged by size:
2115 1445 780 217
StyI C'CwwG_G
Cuts at: 0 374 725 890 917 1079 1386 1448 2146
Size: 374 351 165 27 162 307 62 698
Cuts at: 2146 2253 3419 4557
Size: 107 1166 1138
Fragments arranged by size:
1166 1138 698 374 351 307 165 162
107 62 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1525 1696 1761 2533 2843 4557
Size: 1525 171 65 772 310 1714
Fragments arranged by size:
1714 1525 772 310 171 65
TatI w'GTAC_w
Cuts at: 0 131 4557
Size: 131 4426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 2581 2613 3459 3491 3601 3633
Size: 1051 32 1498 32 846 32 110 32
Cuts at: 3633 3862 3894 4557
Size: 229 32 663
Fragments arranged by size:
1498 1051 846 663 229 110 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1558 4557
Size: 1558 2999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2316 2335 3829 4341 4557
Size: 2316 19 1494 512 216
Fragments arranged by size:
2316 1494 512 216 19
XmaI C'CCGG_G
Cuts at: 0 681 1812 2155 2483 2772 3062 3677 3692
Size: 681 1131 343 328 289 290 615 15
Cuts at: 3692 3923 4557
Size: 231 634
Fragments arranged by size:
1131 681 634 615 343 328 290 289
231 15
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI FspI FspAI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SanDI SexAI SfcI SfiI SfoI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI ApaLI AscI
AseI AsiSI BaeI BciVI BclI BfrBI BglII BmtI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I ClaI DraI EcoRI EcoRV HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SgfI
SgrAI SnaBI SrfI SspI SwaI XbaI XhoI XmnI
ZraI