Restriction map for
pF1KSDA0287
(Linear) (Six-base) MAPSORT of: KIAA0287.seq Check: 2350 from: 1 to: 4392
KIAA0287.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AatII G_ACGT'C
Cuts at: 0 4331 4392
Size: 4331 61
AccI GT'mk_AC
Cuts at: 0 1931 2041 4392
Size: 1931 110 2351
Fragments arranged by size:
2351 1931 110
AclI AA'CG_TT
Cuts at: 0 1137 4392
Size: 1137 3255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 527 1270 2123 2718 3914 4043 4064
Size: 431 96 743 853 595 1196 129 21
Cuts at: 4064 4392
Size: 328
Fragments arranged by size:
1196 853 743 595 431 328 129 96
21
AgeI A'CCGG_T
Cuts at: 0 635 4392
Size: 635 3757
AhdI GACnn_n'nnGTC
Cuts at: 0 162 2864 4356 4392
Size: 162 2702 1492 36
Fragments arranged by size:
2702 1492 162 36
AlwNI CAG_nnn'CTG
Cuts at: 0 3724 4392
Size: 3724 668
ApaI G_GGCC'C
Cuts at: 0 2015 4392
Size: 2015 2377
ApoI r'AATT_y
Cuts at: 0 577 1024 1309 1742 2389 4392
Size: 577 447 285 433 647 2003
Fragments arranged by size:
2003 647 577 447 433 285
AvaI C'yCGr_G
Cuts at: 0 2519 4392
Size: 2519 1873
BanI G'GyrC_C
Cuts at: 0 4291 4392
Size: 4291 101
BanII G_rGCy'C
Cuts at: 0 835 1727 2015 3551 3599 3923 4392
Size: 835 892 288 1536 48 324 469
Fragments arranged by size:
1536 892 835 469 324 288 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 419 2309 2765 2888 2918 4010 4097
Size: 20 399 1890 456 123 30 1092 87
Cuts at: 4097 4392
Size: 295
Fragments arranged by size:
1890 1092 456 399 295 123 87 30
20
BbvCI CC'TCA_GC
Cuts at: 0 329 928 3404 4392
Size: 329 599 2476 988
Fragments arranged by size:
2476 988 599 329
BclI T'GATC_A
Cuts at: 0 4217 4392
Size: 4217 175
BfrBI ATG'CAT
Cuts at: 0 736 1047 4392
Size: 736 311 3345
Fragments arranged by size:
3345 736 311
BglI GCCn_nnn'nGGC
Cuts at: 0 1186 4392
Size: 1186 3206
BlpI GC'TnA_GC
Cuts at: 0 1971 3426 3812 3854 3875 4392
Size: 1971 1455 386 42 21 517
Fragments arranged by size:
1971 1455 517 386 42 21
Bme1580I G_kGCm'C
Cuts at: 0 2015 4392
Size: 2015 2377
BmgBI CAC'GTC
Cuts at: 0 33 4392
Size: 33 4359
BmrI ACTGGGnnnn_n'
Cuts at: 0 126 2009 4392
Size: 126 1883 2383
Fragments arranged by size:
2383 1883 126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 2545 2631 4392
Size: 1078 1467 86 1761
Fragments arranged by size:
1761 1467 1078 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 2108 2375 3122 3406 4392
Size: 252 1856 267 747 284 986
Fragments arranged by size:
1856 986 747 284 267 252
Bpu10I CC'TnA_GC
Cuts at: 0 329 919 928 3404 4254 4392
Size: 329 590 9 2476 850 138
Fragments arranged by size:
2476 850 590 329 138 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2801 4392
Size: 2801 1591
BsaI GGTCTCn'nnnn_
Cuts at: 0 149 1156 2464 2836 2851 4392
Size: 149 1007 1308 372 15 1541
Fragments arranged by size:
1541 1308 1007 372 149 15
BsaAI yAC'GTr
Cuts at: 0 3528 4392
Size: 3528 864
BsaHI Gr'CG_yC
Cuts at: 0 1650 4328 4392
Size: 1650 2678 64
Fragments arranged by size:
2678 1650 64
BsaWI w'CCGG_w
Cuts at: 0 122 635 4392
Size: 122 513 3757
Fragments arranged by size:
3757 513 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 3246 3276 3550 3580 4392
Size: 250 30 2966 30 274 30 812
Fragments arranged by size:
2966 812 274 250 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2824 2854 2869 4392
Size: 2824 30 15 1523
Fragments arranged by size:
2824 1523 30 15
BseYI C'CCAG_C
Cuts at: 0 1456 1461 2401 3289 3507 4392
Size: 1456 5 940 888 218 885
Fragments arranged by size:
1456 940 888 885 218 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1341 3500 4013 4392
Size: 421 920 2159 513 379
Fragments arranged by size:
2159 920 513 421 379
BsiEI CG_ry'CG
Cuts at: 0 636 4392
Size: 636 3756
BsiHKAI G_wGCw'C
Cuts at: 0 1037 1370 1727 2588 4255 4392
Size: 1037 333 357 861 1667 137
Fragments arranged by size:
1667 1037 861 357 333 137
BsmI GAATG_Cn'
Cuts at: 0 738 3631 4159 4183 4392
Size: 738 2893 528 24 209
Fragments arranged by size:
2893 738 528 209 24
Bsp1286I G_dGCh'C
Cuts at: 0 835 1037 1370 1727 2015 2588 3551 3599
Size: 835 202 333 357 288 573 963 48
Cuts at: 3599 3923 4255 4392
Size: 324 332 137
Fragments arranged by size:
963 835 573 357 333 332 324 288
202 137 48
BspHI T'CATG_A
Cuts at: 0 2598 4392
Size: 2598 1794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3985 4392
Size: 3985 407
BsrDI GCAATG_nn'
Cuts at: 0 980 2472 4392
Size: 980 1492 1920
Fragments arranged by size:
1920 1492 980
BsrFI r'CCGG_y
Cuts at: 0 527 635 4392
Size: 527 108 3757
Fragments arranged by size:
3757 527 108
BssSI C'ACGA_G
Cuts at: 0 152 2432 4392
Size: 152 2280 1960
Fragments arranged by size:
2280 1960 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3023 3517 3724 4392
Size: 3023 494 207 668
Fragments arranged by size:
3023 668 494 207
BstEII G'GTnAC_C
Cuts at: 0 106 4392
Size: 106 4286
BstYI r'GATC_y
Cuts at: 0 204 454 2362 2979 4392
Size: 204 250 1908 617 1413
Fragments arranged by size:
1908 1413 617 250 204
BtgI C'CryG_G
Cuts at: 0 199 558 2862 3250 4392
Size: 199 359 2304 388 1142
Fragments arranged by size:
2304 1142 388 359 199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3618 3653 4392
Size: 3618 35 739
Fragments arranged by size:
3618 739 35
DraIII CAC_nnn'GTG
Cuts at: 0 564 1095 4392
Size: 564 531 3297
Fragments arranged by size:
3297 564 531
EaeI y'GGCC_r
Cuts at: 0 420 1695 3296 4392
Size: 420 1275 1601 1096
Fragments arranged by size:
1601 1275 1096 420
EarI CTCTTCn'nnn_
Cuts at: 0 60 609 1819 3455 4019 4040 4348 4392
Size: 60 549 1210 1636 564 21 308 44
Fragments arranged by size:
1636 1210 564 549 308 60 44 21
EcoICRI GAG'CTC
Cuts at: 0 1725 4392
Size: 1725 2667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 431 527 1270 2108 2123 2375 2718
Size: 252 179 96 743 838 15 252 343
Cuts at: 2718 3122 3406 3914 4043 4064 4392
Size: 404 284 508 129 21 328
Fragments arranged by size:
838 743 508 404 343 328 284 252
252 179 129 96 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 2273 4392
Size: 328 1945 2119
Fragments arranged by size:
2119 1945 328
EcoO109I rG'GnC_Cy
Cuts at: 0 2011 3563 4392
Size: 2011 1552 829
Fragments arranged by size:
2011 1552 829
EcoRI G'AATT_C
Cuts at: 0 2389 4392
Size: 2389 2003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 888 1356 1779 4392
Size: 888 468 423 2613
Fragments arranged by size:
2613 888 468 423
HaeII r_GCGC'y
Cuts at: 0 807 4392
Size: 807 3585
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 250 271 282 286 303 318
Size: 148 32 70 21 11 4 17 15
Cuts at: 318 409 441 792 824 828 860 1927 1959
Size: 91 32 351 32 4 32 1067 32
Cuts at: 1959 2850 2882 3547 3579 4342 4374 4392
Size: 891 32 665 32 763 32 18
Fragments arranged by size:
1067 891 763 665 351 148 91 70
32 32 32 32 32 32 32 32
21 18 17 15 11 4 4
HincII GTy'rAC
Cuts at: 0 3033 3504 4392
Size: 3033 471 888
Fragments arranged by size:
3033 888 471
HindIII A'AGCT_T
Cuts at: 0 234 4392
Size: 234 4158
MfeI C'AATT_G
Cuts at: 0 959 2876 4392
Size: 959 1917 1516
Fragments arranged by size:
1917 1516 959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1107 4392
Size: 1107 3285
MscI TGG'CCA
Cuts at: 0 422 4392
Size: 422 3970
MslI CAynn'nnrTG
Cuts at: 0 907 984 1418 3542 4392
Size: 907 77 434 2124 850
Fragments arranged by size:
2124 907 850 434 77
MspA1I CmG'CkG
Cuts at: 0 161 201 717 3111 3724 4392
Size: 161 40 516 2394 613 668
Fragments arranged by size:
2394 668 613 516 161 40
NcoI C'CATG_G
Cuts at: 0 2862 4392
Size: 2862 1530
NdeI CA'TA_TG
Cuts at: 0 981 1947 3356 4392
Size: 981 966 1409 1036
Fragments arranged by size:
1409 1036 981 966
NsiI A_TGCA'T
Cuts at: 0 738 1049 4392
Size: 738 311 3343
Fragments arranged by size:
3343 738 311
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 1095 3130 4392
Size: 315 780 2035 1262
Fragments arranged by size:
2035 1262 780 315
PfoI T'CCnGG_A
Cuts at: 0 356 2352 4392
Size: 356 1996 2040
Fragments arranged by size:
2040 1996 356
PpuMI rG'GwC_Cy
Cuts at: 0 3563 4392
Size: 3563 829
PspOMI G'GGCC_C
Cuts at: 0 2011 4392
Size: 2011 2381
PspXI vC'TCGA_Gb
Cuts at: 0 2519 4392
Size: 2519 1873
PstI C_TGCA'G
Cuts at: 0 3270 3729 3918 4392
Size: 3270 459 189 474
Fragments arranged by size:
3270 474 459 189
PvuII CAG'CTG
Cuts at: 0 3111 3724 4392
Size: 3111 613 668
Fragments arranged by size:
3111 668 613
SacI G_AGCT'C
Cuts at: 0 1727 4392
Size: 1727 2665
SacII CC_GC'GG
Cuts at: 0 202 4392
Size: 202 4190
SapI GCTCTTCn'nnn_
Cuts at: 0 609 4019 4040 4348 4392
Size: 609 3410 21 308 44
Fragments arranged by size:
3410 609 308 44 21
ScaI AGT'ACT
Cuts at: 0 4315 4392
Size: 4315 77
SfcI C'TryA_G
Cuts at: 0 375 3266 3725 3914 4392
Size: 375 2891 459 189 478
Fragments arranged by size:
2891 478 459 375 189
SgrAI Cr'CCGG_yG
Cuts at: 0 527 4392
Size: 527 3865
SmlI C'TyrA_G
Cuts at: 0 2519 2816 4392
Size: 2519 297 1576
Fragments arranged by size:
2519 1576 297
StyI C'CwwG_G
Cuts at: 0 1184 2862 4392
Size: 1184 1678 1530
Fragments arranged by size:
1678 1530 1184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3082 3472 4392
Size: 3082 390 920
Fragments arranged by size:
3082 920 390
TatI w'GTAC_w
Cuts at: 0 3044 4313 4392
Size: 3044 1269 79
Fragments arranged by size:
3044 1269 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 632 664 3055 3087 3246 3278 4392
Size: 632 32 2391 32 159 32 1114
Fragments arranged by size:
2391 1114 632 159 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2308 2887 4392
Size: 2308 579 1505
Fragments arranged by size:
2308 1505 579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 100 158 4392
Size: 100 58 4234
Fragments arranged by size:
4234 100 58
XhoI C'TCGA_G
Cuts at: 0 2519 4392
Size: 2519 1873
XmnI GAAnn'nnTTC
Cuts at: 0 4315 4392
Size: 4315 77
ZraI GAC'GTC
Cuts at: 0 4329 4392
Size: 4329 63
Enzymes that do cut:
AatII AccI AclI AcuI AgeI AhdI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BbvCI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BssSI BstAPI BstEII BstYI BtgI CspCI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI PflMI PfoI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SapI ScaI SfcI
SgrAI SmlI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AflIII AleI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BglII BmtI BsaBI BsiWI BsmBI BspEI
BsrBI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI DraI DrdI EagI EciI EcoRV FseI
FspI FspAI HpaI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XmaI