Restriction map for
pF1KSDA0289
(Linear) (Six-base) MAPSORT of: KIAA0289.seq Check: 2725 from: 1 to: 3882
KIAA0289.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 523 3882
Size: 523 3359
Acc65I G'GTAC_C
Cuts at: 0 2405 2612 3882
Size: 2405 207 1270
Fragments arranged by size:
2405 1270 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1055 2373 3691 3882
Size: 431 624 1318 1318 191
Fragments arranged by size:
1318 1318 624 431 191
AflIII A'CryG_T
Cuts at: 0 3359 3823 3882
Size: 3359 464 59
Fragments arranged by size:
3359 464 59
AleI CACnn'nnGTG
Cuts at: 0 1421 3112 3882
Size: 1421 1691 770
Fragments arranged by size:
1691 1421 770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1277 1645 3882
Size: 1277 368 2237
Fragments arranged by size:
2237 1277 368
AlwNI CAG_nnn'CTG
Cuts at: 0 336 1316 2232 2283 2983 3840 3882
Size: 336 980 916 51 700 857 42
Fragments arranged by size:
980 916 857 700 336 51 42
ApoI r'AATT_y
Cuts at: 0 1839 3610 3882
Size: 1839 1771 272
Fragments arranged by size:
1839 1771 272
AvaI C'yCGr_G
Cuts at: 0 222 3882
Size: 222 3660
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3730 3763 3882
Size: 3730 33 119
Fragments arranged by size:
3730 119 33
BamHI G'GATC_C
Cuts at: 0 81 449 1089 1470 3882
Size: 81 368 640 381 2412
Fragments arranged by size:
2412 640 381 368 81
BanI G'GyrC_C
Cuts at: 0 379 685 2004 2405 2612 2761 3294 3755
Size: 379 306 1319 401 207 149 533 461
Cuts at: 3755 3882
Size: 127
Fragments arranged by size:
1319 533 461 401 379 306 207 149
127
BanII G_rGCy'C
Cuts at: 0 17 191 2351 2965 3101 3803 3882
Size: 17 174 2160 614 136 702 79
Fragments arranged by size:
2160 702 614 174 136 79 17
BbeI G_GCGC'C
Cuts at: 0 2008 3882
Size: 2008 1874
BbsI GAAGACnn'nnnn_
Cuts at: 0 2663 3430 3826 3882
Size: 2663 767 396 56
Fragments arranged by size:
2663 767 396 56
BbvCI CC'TCA_GC
Cuts at: 0 445 584 1636 2485 3682 3882
Size: 445 139 1052 849 1197 200
Fragments arranged by size:
1197 1052 849 445 200 139
BciVI GTATCCnnnnn_n'
Cuts at: 0 3856 3882
Size: 3856 26
BclI T'GATC_A
Cuts at: 0 1163 3326 3882
Size: 1163 2163 556
Fragments arranged by size:
2163 1163 556
BglI GCCn_nnn'nGGC
Cuts at: 0 1669 3882
Size: 1669 2213
BglII A'GATC_T
Cuts at: 0 284 2339 3476 3882
Size: 284 2055 1137 406
Fragments arranged by size:
2055 1137 406 284
BlpI GC'TnA_GC
Cuts at: 0 2867 3235 3802 3882
Size: 2867 368 567 80
Fragments arranged by size:
2867 567 368 80
Bme1580I G_kGCm'C
Cuts at: 0 384 635 1392 1891 2315 3882
Size: 384 251 757 499 424 1567
Fragments arranged by size:
1567 757 499 424 384 251
BmgBI CAC'GTC
Cuts at: 0 78 1209 3414 3824 3882
Size: 78 1131 2205 410 58
Fragments arranged by size:
2205 1131 410 78 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 1445 2384 3801 3882
Size: 1445 939 1417 81
Fragments arranged by size:
1445 1417 939 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1596 2358 3882
Size: 1596 762 1524
Fragments arranged by size:
1596 1524 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 271 301 342 358 663 957 1309
Size: 118 153 30 41 16 305 294 352
Cuts at: 1309 1344 1792 1978 2338 2668 2801 2902 3005
Size: 35 448 186 360 330 133 101 103
Cuts at: 3005 3142 3281 3384 3882
Size: 137 139 103 498
Fragments arranged by size:
498 448 360 352 330 305 294 186
153 139 137 133 118 103 103 101
41 35 30 16
Bpu10I CC'TnA_GC
Cuts at: 0 157 445 584 691 1250 1636 2485 2976
Size: 157 288 139 107 559 386 849 491
Cuts at: 2976 3682 3882
Size: 706 200
Fragments arranged by size:
849 706 559 491 386 288 200 157
139 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3443 3727 3882
Size: 3443 284 155
Fragments arranged by size:
3443 284 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 2104 2702 3661 3882
Size: 2104 598 959 221
Fragments arranged by size:
2104 959 598 221
BsaBI GATnn'nnATC
Cuts at: 0 1168 3882
Size: 1168 2714
BsaHI Gr'CG_yC
Cuts at: 0 2005 3882
Size: 2005 1877
BsaWI w'CCGG_w
Cuts at: 0 2432 3882
Size: 2432 1450
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 218 248 1098 1128 1609 1639
Size: 85 30 103 30 850 30 481 30
Cuts at: 1639 3882
Size: 2243
Fragments arranged by size:
2243 850 481 103 85 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 454 1191 1753 2350 2608 2710 3163
Size: 187 267 737 562 597 258 102 453
Cuts at: 3163 3844 3882
Size: 681 38
Fragments arranged by size:
737 681 597 562 453 267 258 187
102 38
BseYI C'CCAG_C
Cuts at: 0 38 1070 3100 3668 3882
Size: 38 1032 2030 568 214
Fragments arranged by size:
2030 1032 568 214 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 2546 3175 3882
Size: 685 1861 629 707
Fragments arranged by size:
1861 707 685 629
BsiEI CG_ry'CG
Cuts at: 0 68 3882
Size: 68 3814
BsiHKAI G_wGCw'C
Cuts at: 0 1201 1256 2965 2977 3803 3882
Size: 1201 55 1709 12 826 79
Fragments arranged by size:
1709 1201 826 79 55 12
BsmI GAATG_Cn'
Cuts at: 0 1447 1493 3882
Size: 1447 46 2389
Fragments arranged by size:
2389 1447 46
Bsp1286I G_dGCh'C
Cuts at: 0 17 191 384 635 1201 1256 1392 1891
Size: 17 174 193 251 566 55 136 499
Cuts at: 1891 2315 2351 2965 2977 3101 3803 3882
Size: 424 36 614 12 124 702 79
Fragments arranged by size:
702 614 566 499 424 251 193 174
136 124 79 55 36 17 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2616 3882
Size: 2616 1266
BsrBI CCG'CTC
Cuts at: 0 2700 3882
Size: 2700 1182
BsrDI GCAATG_nn'
Cuts at: 0 660 840 2496 3287 3702 3882
Size: 660 180 1656 791 415 180
Fragments arranged by size:
1656 791 660 415 180 180
BsrFI r'CCGG_y
Cuts at: 0 45 70 530 1797 2871 3882
Size: 45 25 460 1267 1074 1011
Fragments arranged by size:
1267 1074 1011 460 45 25
BssSI C'ACGA_G
Cuts at: 0 1192 2264 2522 3094 3882
Size: 1192 1072 258 572 788
Fragments arranged by size:
1192 1072 788 572 258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2795 3692 3882
Size: 2795 897 190
Fragments arranged by size:
2795 897 190
BstEII G'GTnAC_C
Cuts at: 0 2886 3882
Size: 2886 996
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 2214 2772 3882
Size: 96 2118 558 1110
Fragments arranged by size:
2118 1110 558 96
BstYI r'GATC_y
Cuts at: 0 81 284 449 1089 1470 2339 3476 3543
Size: 81 203 165 640 381 869 1137 67
Cuts at: 3543 3882
Size: 339
Fragments arranged by size:
1137 869 640 381 339 203 165 81
67
BstZ17I GTA'TAC
Cuts at: 0 524 3882
Size: 524 3358
Bsu36I CC'TnA_GG
Cuts at: 0 2714 3814 3882
Size: 2714 1100 68
Fragments arranged by size:
2714 1100 68
BtgI C'CryG_G
Cuts at: 0 62 1376 1525 3086 3437 3882
Size: 62 1314 149 1561 351 445
Fragments arranged by size:
1561 1314 445 351 149 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3636 3882
Size: 3636 246
BtsI GCAGTG_nn'
Cuts at: 0 130 337 3020 3882
Size: 130 207 2683 862
Fragments arranged by size:
2683 862 207 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 663 698 3009 3044 3882
Size: 663 35 2311 35 838
Fragments arranged by size:
2311 838 663 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 3371 3882
Size: 3371 511
EaeI y'GGCC_r
Cuts at: 0 59 65 1670 2303 2468 3882
Size: 59 6 1605 633 165 1414
Fragments arranged by size:
1605 1414 633 165 59 6
EagI C'GGCC_G
Cuts at: 0 65 3882
Size: 65 3817
EarI CTCTTCn'nnn_
Cuts at: 0 205 431 565 1348 3493 3882
Size: 205 226 134 783 2145 389
Fragments arranged by size:
2145 783 389 226 205 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1008 3811 3882
Size: 1008 2803 71
Fragments arranged by size:
2803 1008 71
EcoICRI GAG'CTC
Cuts at: 0 2963 3801 3882
Size: 2963 838 81
Fragments arranged by size:
2963 838 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 271 301 342 358 431 663 957
Size: 118 153 30 41 16 73 232 294
Cuts at: 957 1055 1309 1344 1792 1978 2338 2373 2668
Size: 98 254 35 448 186 360 35 295
Cuts at: 2668 2801 2902 3005 3142 3281 3384 3691 3882
Size: 133 101 103 137 139 103 307 191
Fragments arranged by size:
448 360 307 295 294 254 232 191
186 153 139 137 133 118 103 103
101 98 73 41 35 35 30 16
EcoO109I rG'GnC_Cy
Cuts at: 0 555 2481 3570 3640 3678 3810 3882
Size: 555 1926 1089 70 38 132 72
Fragments arranged by size:
1926 1089 555 132 72 70 38
EcoRI G'AATT_C
Cuts at: 0 3610 3882
Size: 3610 272
EcoRV GAT'ATC
Cuts at: 0 306 3882
Size: 306 3576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 216 973 1170 3741 3882
Size: 216 757 197 2571 141
Fragments arranged by size:
2571 757 216 197 141
FspI TGC'GCA
Cuts at: 0 211 652 3882
Size: 211 441 3230
Fragments arranged by size:
3230 441 211
HaeII r_GCGC'y
Cuts at: 0 537 2008 2542 3399 3882
Size: 537 1471 534 857 483
Fragments arranged by size:
1471 857 537 534 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 2074 2106 2978 3010 3241 3273
Size: 218 32 1824 32 872 32 231 32
Cuts at: 3273 3468 3500 3882
Size: 195 32 382
Fragments arranged by size:
1824 872 382 231 218 195 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1212 3882
Size: 1212 2670
HindIII A'AGCT_T
Cuts at: 0 2071 3723 3882
Size: 2071 1652 159
Fragments arranged by size:
2071 1652 159
KasI G'GCGC_C
Cuts at: 0 2004 3882
Size: 2004 1878
KpnI G_GTAC'C
Cuts at: 0 2409 2616 3882
Size: 2409 207 1266
Fragments arranged by size:
2409 1266 207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1982 2525 3882
Size: 962 1020 543 1357
Fragments arranged by size:
1357 1020 962 543
MscI TGG'CCA
Cuts at: 0 61 1672 2305 2470 3882
Size: 61 1611 633 165 1412
Fragments arranged by size:
1611 1412 633 165 61
MslI CAynn'nnrTG
Cuts at: 0 121 374 1421 1708 1945 2041 3112 3358
Size: 121 253 1047 287 237 96 1071 246
Cuts at: 3358 3882
Size: 524
Fragments arranged by size:
1071 1047 524 287 253 246 237 121
96
MspA1I CmG'CkG
Cuts at: 0 1074 1178 1302 1364 1847 1986 2634 3882
Size: 1074 104 124 62 483 139 648 1248
Fragments arranged by size:
1248 1074 648 483 139 124 104 62
NaeI GCC'GGC
Cuts at: 0 47 72 532 3882
Size: 47 25 460 3350
Fragments arranged by size:
3350 460 47 25
NarI GG'CG_CC
Cuts at: 0 2005 3882
Size: 2005 1877
NcoI C'CATG_G
Cuts at: 0 1376 1525 3882
Size: 1376 149 2357
Fragments arranged by size:
2357 1376 149
NgoMIV G'CCGG_C
Cuts at: 0 45 70 530 3882
Size: 45 25 460 3352
Fragments arranged by size:
3352 460 45 25
NspI r_CATG'y
Cuts at: 0 1246 1626 3363 3882
Size: 1246 380 1737 519
Fragments arranged by size:
1737 1246 519 380
PasI CC'CwG_GG
Cuts at: 0 1370 3882
Size: 1370 2512
PciI A'CATG_T
Cuts at: 0 3359 3882
Size: 3359 523
PflMI CCAn_nnn'nTGG
Cuts at: 0 1316 2001 2505 3882
Size: 1316 685 504 1377
Fragments arranged by size:
1377 1316 685 504
PfoI T'CCnGG_A
Cuts at: 0 393 698 713 1400 2012 2570 3119 3539
Size: 393 305 15 687 612 558 549 420
Cuts at: 3539 3882
Size: 343
Fragments arranged by size:
687 612 558 549 420 393 343 305
15
PpuMI rG'GwC_Cy
Cuts at: 0 555 3570 3640 3678 3810 3882
Size: 555 3015 70 38 132 72
Fragments arranged by size:
3015 555 132 72 70 38
PshAI GACnn'nnGTC
Cuts at: 0 3005 3882
Size: 3005 877
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 3882
Size: 1107 32 2743
Fragments arranged by size:
2743 1107 32
PstI C_TGCA'G
Cuts at: 0 761 827 1813 1834 2420 3882
Size: 761 66 986 21 586 1462
Fragments arranged by size:
1462 986 761 586 66 21
PvuII CAG'CTG
Cuts at: 0 1074 1178 1302 1986 3882
Size: 1074 104 124 684 1896
Fragments arranged by size:
1896 1074 684 124 104
SacI G_AGCT'C
Cuts at: 0 2965 3803 3882
Size: 2965 838 79
Fragments arranged by size:
2965 838 79
SanDI GG'GwC_CC
Cuts at: 0 555 3882
Size: 555 3327
SbfI CC_TGCA'GG
Cuts at: 0 827 3882
Size: 827 3055
SexAI A'CCwGG_T
Cuts at: 0 1559 3882
Size: 1559 2323
SfcI C'TryA_G
Cuts at: 0 757 823 1809 1830 2416 3762 3882
Size: 757 66 986 21 586 1346 120
Fragments arranged by size:
1346 986 757 586 120 66 21
SfoI GGC'GCC
Cuts at: 0 2006 3882
Size: 2006 1876
SgrAI Cr'CCGG_yG
Cuts at: 0 70 530 3882
Size: 70 460 3352
Fragments arranged by size:
3352 460 70
SmaI CCC'GGG
Cuts at: 0 224 3882
Size: 224 3658
SmlI C'TyrA_G
Cuts at: 0 3458 3742 3882
Size: 3458 284 140
Fragments arranged by size:
3458 284 140
SpeI A'CTAG_T
Cuts at: 0 2913 3502 3882
Size: 2913 589 380
Fragments arranged by size:
2913 589 380
SspI AAT'ATT
Cuts at: 0 912 1541 1592 3882
Size: 912 629 51 2290
Fragments arranged by size:
2290 912 629 51
StyI C'CwwG_G
Cuts at: 0 89 1376 1525 2207 3882
Size: 89 1287 149 682 1675
Fragments arranged by size:
1675 1287 682 149 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 2042 3882
Size: 460 1582 1840
Fragments arranged by size:
1840 1582 460
TatI w'GTAC_w
Cuts at: 0 1476 3882
Size: 1476 2406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 122 154 1609 1641 3810 3842
Size: 85 32 5 32 1455 32 2169 32
Cuts at: 3842 3882
Size: 40
Fragments arranged by size:
2169 1455 85 40 32 32 32 32
5
Tth111I GACn'n_nGTC
Cuts at: 0 1901 3882
Size: 1901 1981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 679 927 2170 2313 2506 3882
Size: 504 175 248 1243 143 193 1376
Fragments arranged by size:
1376 1243 504 248 193 175 143
XmaI C'CCGG_G
Cuts at: 0 222 3882
Size: 222 3660
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AlwNI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PshAI
PsrI PstI PvuII SacI SanDI SbfI SexAI SfcI
SfoI SgrAI SmaI SmlI SpeI SspI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BcgI BfrBI
BmtI BsaAI BsiWI BsmBI BspEI BspHI BsrGI BssHII
BstBI ClaI DraI DrdI EcoNI FseI FspAI HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SapI ScaI SfiI SgfI SnaBI SphI
SrfI StuI SwaI XbaI XhoI XmnI ZraI