Restriction map for
pF1KA0292
(Linear) (Six-base) MAPSORT of: KIAA0292.seq Check: 9710 from: 1 to: 5880
KIAA0292.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 0 991 5880
Size: 991 4889
Acc65I G'GTAC_C
Cuts at: 0 1326 5880
Size: 1326 4554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1074 1523 1541 1552 2254 3854 4199 4283
Size: 1074 449 18 11 702 1600 345 84
Cuts at: 4283 4553 5477 5880
Size: 270 924 403
Fragments arranged by size:
1600 1074 924 702 449 403 345 270
84 18 11
AfeI AGC'GCT
Cuts at: 0 5633 5880
Size: 5633 247
AflIII A'CryG_T
Cuts at: 0 1415 5880
Size: 1415 4465
AhdI GACnn_n'nnGTC
Cuts at: 0 2626 5880
Size: 2626 3254
AleI CACnn'nnGTG
Cuts at: 0 2663 5261 5880
Size: 2663 2598 619
Fragments arranged by size:
2663 2598 619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1506 5279 5880
Size: 1506 3773 601
Fragments arranged by size:
3773 1506 601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3291 3323 5880
Size: 3291 32 2557
Fragments arranged by size:
3291 2557 32
AlwNI CAG_nnn'CTG
Cuts at: 0 190 193 579 1351 2485 4363 4860 5437
Size: 190 3 386 772 1134 1878 497 577
Cuts at: 5437 5880
Size: 443
Fragments arranged by size:
1878 1134 772 577 497 443 386 190
3
ApaI G_GGCC'C
Cuts at: 0 2068 2455 4773 5880
Size: 2068 387 2318 1107
Fragments arranged by size:
2318 2068 1107 387
ApoI r'AATT_y
Cuts at: 0 117 894 1229 1497 2242 3237 5551 5880
Size: 117 777 335 268 745 995 2314 329
Fragments arranged by size:
2314 995 777 745 335 329 268 117
AvaI C'yCGr_G
Cuts at: 0 1826 3200 5880
Size: 1826 1374 2680
Fragments arranged by size:
2680 1826 1374
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3250 3283 5880
Size: 3250 33 2597
Fragments arranged by size:
3250 2597 33
BanI G'GyrC_C
Cuts at: 0 1326 4384 5102 5363 5680 5783 5880
Size: 1326 3058 718 261 317 103 97
Fragments arranged by size:
3058 1326 718 317 261 103 97
BanII G_rGCy'C
Cuts at: 0 2068 2455 2646 3207 3403 4773 5417 5880
Size: 2068 387 191 561 196 1370 644 463
Fragments arranged by size:
2068 1370 644 561 463 387 196 191
BbeI G_GCGC'C
Cuts at: 0 5367 5684 5880
Size: 5367 317 196
Fragments arranged by size:
5367 317 196
BbsI GAAGACnn'nnnn_
Cuts at: 0 701 1456 1810 2798 4289 4342 5081 5384
Size: 701 755 354 988 1491 53 739 303
Cuts at: 5384 5880
Size: 496
Fragments arranged by size:
1491 988 755 739 701 496 354 303
53
BbvCI CC'TCA_GC
Cuts at: 0 320 3671 4682 4955 5854 5880
Size: 320 3351 1011 273 899 26
Fragments arranged by size:
3351 1011 899 320 273 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 5880
Size: 26 5854
BciVI GTATCCnnnnn_n'
Cuts at: 0 2189 5880
Size: 2189 3691
BfrBI ATG'CAT
Cuts at: 0 1948 5880
Size: 1948 3932
BglI GCCn_nnn'nGGC
Cuts at: 0 336 2048 2964 3475 4516 5880
Size: 336 1712 916 511 1041 1364
Fragments arranged by size:
1712 1364 1041 916 511 336
Bme1580I G_kGCm'C
Cuts at: 0 2068 2455 3471 4773 5105 5486 5788 5815
Size: 2068 387 1016 1302 332 381 302 27
Cuts at: 5815 5880
Size: 65
Fragments arranged by size:
2068 1302 1016 387 381 332 302 65
27
BmrI ACTGGGnnnn_n'
Cuts at: 0 479 1184 1271 2457 2662 3458 3788 5880
Size: 479 705 87 1186 205 796 330 2092
Fragments arranged by size:
2092 1186 796 705 479 330 205 87
BmtI G_CTAG'C
Cuts at: 0 212 2399 5880
Size: 212 2187 3481
Fragments arranged by size:
3481 2187 212
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1761 4190 5790 5880
Size: 1761 2429 1600 90
Fragments arranged by size:
2429 1761 1600 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 467 1097 1171 1554 1955 2861 2921
Size: 278 189 630 74 383 401 906 60
Cuts at: 2921 3318 3572 3593 4478 5533 5880
Size: 397 254 21 885 1055 347
Fragments arranged by size:
1055 906 885 630 401 397 383 347
278 254 189 74 60 21
Bpu10I CC'TnA_GC
Cuts at: 0 320 1757 3314 3671 4682 4955 5507 5789
Size: 320 1437 1557 357 1011 273 552 282
Cuts at: 5789 5854 5880
Size: 65 26
Fragments arranged by size:
1557 1437 1011 552 357 320 282 273
65 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 2300 2798 5880
Size: 1367 933 498 3082
Fragments arranged by size:
3082 1367 933 498
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 2432 2837 3077 4425 4617 5880
Size: 208 2224 405 240 1348 192 1263
Fragments arranged by size:
2224 1348 1263 405 240 208 192
BsaAI yAC'GTr
Cuts at: 0 4275 5880
Size: 4275 1605
BsaHI Gr'CG_yC
Cuts at: 0 5364 5681 5880
Size: 5364 317 199
Fragments arranged by size:
5364 317 199
BsaWI w'CCGG_w
Cuts at: 0 3131 5141 5880
Size: 3131 2010 739
Fragments arranged by size:
3131 2010 739
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 384 414 1143 1173 3291 3321 4121 4151
Size: 384 30 729 30 2118 30 800 30
Cuts at: 4151 5880
Size: 1729
Fragments arranged by size:
2118 1729 800 729 384 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 194 308 711 714 717 720 723 1151
Size: 194 114 403 3 3 3 3 428
Cuts at: 1151 1430 1500 1662 1991 1994 3298 3774 4110
Size: 279 70 162 329 3 1304 476 336
Cuts at: 4110 5308 5311 5779 5880
Size: 1198 3 468 101
Fragments arranged by size:
1304 1198 476 468 428 403 336 329
279 194 162 114 101 70 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1958 3478 4123 4772 4853 5880
Size: 1958 1520 645 649 81 1027
Fragments arranged by size:
1958 1520 1027 649 645 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1476 5880
Size: 364 1112 4404
Fragments arranged by size:
4404 1112 364
BsiHKAI G_wGCw'C
Cuts at: 0 495 1374 1624 1692 5880
Size: 495 879 250 68 4188
Fragments arranged by size:
4188 879 495 250 68
BsmI GAATG_Cn'
Cuts at: 0 804 3883 5880
Size: 804 3079 1997
Fragments arranged by size:
3079 1997 804
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2701 5880
Size: 2701 3179
Bsp1286I G_dGCh'C
Cuts at: 0 495 1374 1624 1692 2068 2455 2646 3207
Size: 495 879 250 68 376 387 191 561
Cuts at: 3207 3403 3471 4773 5105 5417 5486 5788 5815
Size: 196 68 1302 332 312 69 302 27
Cuts at: 5815 5880
Size: 65
Fragments arranged by size:
1302 879 561 495 387 376 332 312
302 250 196 191 69 68 68 65
27
BspHI T'CATG_A
Cuts at: 0 3027 3755 5880
Size: 3027 728 2125
Fragments arranged by size:
3027 2125 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3220 5880
Size: 3220 2660
BsrBI CCG'CTC
Cuts at: 0 2774 5372 5874 5880
Size: 2774 2598 502 6
Fragments arranged by size:
2774 2598 502 6
BsrDI GCAATG_nn'
Cuts at: 0 1926 2895 5553 5880
Size: 1926 969 2658 327
Fragments arranged by size:
2658 1926 969 327
BsrFI r'CCGG_y
Cuts at: 0 1677 5880
Size: 1677 4203
BsrGI T'GTAC_A
Cuts at: 0 4939 5880
Size: 4939 941
BstAPI GCAn_nnn'nTGC
Cuts at: 0 190 193 984 1687 2265 5880
Size: 190 3 791 703 578 3615
Fragments arranged by size:
3615 791 703 578 190 3
BstBI TT'CG_AA
Cuts at: 0 4108 5880
Size: 4108 1772
BstEII G'GTnAC_C
Cuts at: 0 232 667 2275 5152 5880
Size: 232 435 1608 2877 728
Fragments arranged by size:
2877 1608 728 435 232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 121 2370 3478 5880
Size: 121 2249 1108 2402
Fragments arranged by size:
2402 2249 1108 121
BstYI r'GATC_y
Cuts at: 0 805 1347 3567 5880
Size: 805 542 2220 2313
Fragments arranged by size:
2313 2220 805 542
BstZ17I GTA'TAC
Cuts at: 0 992 5880
Size: 992 4888
Bsu36I CC'TnA_GG
Cuts at: 0 1049 4514 5880
Size: 1049 3465 1366
Fragments arranged by size:
3465 1366 1049
BtgI C'CryG_G
Cuts at: 0 30 2214 2747 2883 3355 4030 5880
Size: 30 2184 533 136 472 675 1850
Fragments arranged by size:
2184 1850 675 533 472 136 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 217 5880
Size: 217 5663
BtsI GCAGTG_nn'
Cuts at: 0 156 162 168 1209 2847 5454 5664 5880
Size: 156 6 6 1041 1638 2607 210 216
Fragments arranged by size:
2607 1638 1041 216 210 156 6 6
DraIII CAC_nnn'GTG
Cuts at: 0 828 1687 5880
Size: 828 859 4193
Fragments arranged by size:
4193 859 828
EaeI y'GGCC_r
Cuts at: 0 222 594 693 1388 1614 2040 2049 2211
Size: 222 372 99 695 226 426 9 162
Cuts at: 2211 5339 5880
Size: 3128 541
Fragments arranged by size:
3128 695 541 426 372 226 222 162
99 9
EarI CTCTTCn'nnn_
Cuts at: 0 77 1730 3355 4159 4213 4292 4298 4529
Size: 77 1653 1625 804 54 79 6 231
Cuts at: 4529 5297 5880
Size: 768 583
Fragments arranged by size:
1653 1625 804 768 583 231 79 77
54 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3078 5880
Size: 3078 2802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 467 1074 1097 1171 1523 1541 1552
Size: 278 189 607 23 74 352 18 11
Cuts at: 1552 1554 1955 2254 2861 2921 3318 3572 3593
Size: 2 401 299 607 60 397 254 21
Cuts at: 3593 3854 4199 4283 4478 4553 5477 5533 5880
Size: 261 345 84 195 75 924 56 347
Fragments arranged by size:
924 607 607 401 397 352 347 345
299 278 261 254 195 189 84 75
74 60 56 23 21 18 11 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 583 1458 1973 2257 2460 3227 4178 5880
Size: 583 875 515 284 203 767 951 1702
Fragments arranged by size:
1702 951 875 767 583 515 284 203
EcoO109I rG'GnC_Cy
Cuts at: 0 923 1064 1463 2065 2403 2451 2452 2634
Size: 923 141 399 602 338 48 1 182
Cuts at: 2634 2970 3219 3954 4769 5071 5392 5880
Size: 336 249 735 815 302 321 488
Fragments arranged by size:
923 815 735 602 488 399 338 336
321 302 249 182 141 48 1
EcoRI G'AATT_C
Cuts at: 0 3237 5880
Size: 3237 2643
EcoRV GAT'ATC
Cuts at: 0 3921 5880
Size: 3921 1959
HaeII r_GCGC'y
Cuts at: 0 1757 3486 3875 5044 5367 5635 5684 5880
Size: 1757 1729 389 1169 323 268 49 196
Fragments arranged by size:
1757 1729 1169 389 323 268 196 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 384 416 1263 1295 1861 1893 1999 2031
Size: 384 32 847 32 566 32 106 32
Cuts at: 2031 2499 2531 2533 2565 2582 2612 2614 2644
Size: 468 32 2 32 17 30 2 30
Cuts at: 2644 3910 3942 4141 4173 5880
Size: 1266 32 199 32 1707
Fragments arranged by size:
1707 1266 847 566 468 384 199 106
32 32 32 32 32 32 32 32
30 30 17 2 2
KasI G'GCGC_C
Cuts at: 0 5363 5680 5880
Size: 5363 317 200
Fragments arranged by size:
5363 317 200
KpnI G_GTAC'C
Cuts at: 0 1330 5880
Size: 1330 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1802 2042 2728 5849 5880
Size: 1282 520 240 686 3121 31
Fragments arranged by size:
3121 1282 686 520 240 31
MscI TGG'CCA
Cuts at: 0 224 596 695 1390 1616 2042 2051 2213
Size: 224 372 99 695 226 426 9 162
Cuts at: 2213 5880
Size: 3667
Fragments arranged by size:
3667 695 426 372 226 224 162 99
9
MslI CAynn'nnrTG
Cuts at: 0 2663 2959 3212 5230 5261 5880
Size: 2663 296 253 2018 31 619
Fragments arranged by size:
2663 2018 619 296 253 31
MspA1I CmG'CkG
Cuts at: 0 190 636 1117 1530 2062 2885 3246 3305
Size: 190 446 481 413 532 823 361 59
Cuts at: 3305 3602 5880
Size: 297 2278
Fragments arranged by size:
2278 823 532 481 446 413 361 297
190 59
NarI GG'CG_CC
Cuts at: 0 5364 5681 5880
Size: 5364 317 199
Fragments arranged by size:
5364 317 199
NcoI C'CATG_G
Cuts at: 0 2214 2747 5880
Size: 2214 533 3133
Fragments arranged by size:
3133 2214 533
NheI G'CTAG_C
Cuts at: 0 208 2395 5880
Size: 208 2187 3485
Fragments arranged by size:
3485 2187 208
NsiI A_TGCA'T
Cuts at: 0 1950 5880
Size: 1950 3930
NspI r_CATG'y
Cuts at: 0 982 1419 1767 4351 5880
Size: 982 437 348 2584 1529
Fragments arranged by size:
2584 1529 982 437 348
PasI CC'CwG_GG
Cuts at: 0 363 2456 2638 3076 4446 5880
Size: 363 2093 182 438 1370 1434
Fragments arranged by size:
2093 1434 1370 438 363 182
PciI A'CATG_T
Cuts at: 0 1415 5880
Size: 1415 4465
PflMI CCAn_nnn'nTGG
Cuts at: 0 4860 5880
Size: 4860 1020
PfoI T'CCnGG_A
Cuts at: 0 1971 2103 3609 5880
Size: 1971 132 1506 2271
Fragments arranged by size:
2271 1971 1506 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3291 3323 5880
Size: 3291 32 2557
Fragments arranged by size:
3291 2557 32
PpuMI rG'GwC_Cy
Cuts at: 0 923 1064 2634 2970 3219 3954 5071 5880
Size: 923 141 1570 336 249 735 1117 809
Fragments arranged by size:
1570 1117 923 809 735 336 249 141
PsiI TTA'TAA
Cuts at: 0 3254 5880
Size: 3254 2626
PspOMI G'GGCC_C
Cuts at: 0 2064 2451 4769 5880
Size: 2064 387 2318 1111
Fragments arranged by size:
2318 2064 1111 387
PstI C_TGCA'G
Cuts at: 0 1299 1899 2061 2261 3333 4713 5069 5627
Size: 1299 600 162 200 1072 1380 356 558
Cuts at: 5627 5880
Size: 253
Fragments arranged by size:
1380 1299 1072 600 558 356 253 200
162
PvuII CAG'CTG
Cuts at: 0 190 1117 1530 3246 5880
Size: 190 927 413 1716 2634
Fragments arranged by size:
2634 1716 927 413 190
SacII CC_GC'GG
Cuts at: 0 2886 5880
Size: 2886 2994
SanDI GG'GwC_CC
Cuts at: 0 923 2634 2970 3219 5880
Size: 923 1711 336 249 2661
Fragments arranged by size:
2661 1711 923 336 249
SapI GCTCTTCn'nnn_
Cuts at: 0 5297 5880
Size: 5297 583
SbfI CC_TGCA'GG
Cuts at: 0 2261 5627 5880
Size: 2261 3366 253
Fragments arranged by size:
3366 2261 253
ScaI AGT'ACT
Cuts at: 0 1380 2313 5880
Size: 1380 933 3567
Fragments arranged by size:
3567 1380 933
SexAI A'CCwGG_T
Cuts at: 0 3693 5105 5597 5880
Size: 3693 1412 492 283
Fragments arranged by size:
3693 1412 492 283
SfcI C'TryA_G
Cuts at: 0 299 622 1143 1295 1895 2057 2118 2183
Size: 299 323 521 152 600 162 61 65
Cuts at: 2183 2257 3329 4061 4709 5065 5623 5880
Size: 74 1072 732 648 356 558 257
Fragments arranged by size:
1072 732 648 600 558 521 356 323
299 257 162 152 74 65 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 336 2048 5880
Size: 336 1712 3832
Fragments arranged by size:
3832 1712 336
SfoI GGC'GCC
Cuts at: 0 5365 5682 5880
Size: 5365 317 198
Fragments arranged by size:
5365 317 198
SmaI CCC'GGG
Cuts at: 0 1828 5880
Size: 1828 4052
SmlI C'TyrA_G
Cuts at: 0 1382 2315 2813 5880
Size: 1382 933 498 3067
Fragments arranged by size:
3067 1382 933 498
SspI AAT'ATT
Cuts at: 0 4089 5880
Size: 4089 1791
StuI AGG'CCT
Cuts at: 0 330 5880
Size: 330 5550
StyI C'CwwG_G
Cuts at: 0 435 2214 2747 3367 3950 4291 4425 4526
Size: 435 1779 533 620 583 341 134 101
Cuts at: 4526 5687 5880
Size: 1161 193
Fragments arranged by size:
1779 1161 620 583 533 435 341 193
134 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2709 2999 4438 4867 5140 5387 5880
Size: 2709 290 1439 429 273 247 493
Fragments arranged by size:
2709 1439 493 429 290 273 247
TatI w'GTAC_w
Cuts at: 0 1037 1378 2311 4939 5880
Size: 1037 341 933 2628 941
Fragments arranged by size:
2628 1037 941 933 341
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 812 844 5063 5095 5136 5168
Size: 55 32 725 32 4219 32 41 32
Cuts at: 5168 5880
Size: 712
Fragments arranged by size:
4219 725 712 55 41 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 4246 5085 5880
Size: 4246 839 795
Fragments arranged by size:
4246 839 795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3652 4857 5880
Size: 3652 1205 1023
Fragments arranged by size:
3652 1205 1023
XmaI C'CCGG_G
Cuts at: 0 1826 5880
Size: 1826 4054
XmnI GAAnn'nnTTC
Cuts at: 0 330 2267 5880
Size: 330 1937 3613
Fragments arranged by size:
3613 1937 330
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NheI NsiI NspI
PasI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI
PstI PvuII SacII SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI ApaLI AscI AseI
AsiSI AvrII BamHI BclI BglII BlpI BmgBI BsaBI
BsiEI BsiWI BspEI BssHII BssSI ClaI CspCI DraI
DrdI EagI EcoICRI FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NotI
NruI PacI PmeI PmlI PshAI PspXI PsrI PvuI
RsrII SacI SalI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI XbaI XhoI ZraI