Restriction map for pF1KSDA0293
 (Linear) (Six-base) MAPSORT of: KIAA0293.seq  Check: 2096  from: 1  to: 4458



KIAA0293.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1150    4458 
   Size:       1150    3308
 
AatII G_ACGT'C

Cuts at:      0     454    4458 
   Size:        454    4004
 
AccI GT'mk_AC

Cuts at:      0    2687    4458 
   Size:       2687    1771
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1193    2647    3310    3814    4458 
   Size:        961     232    1454     663     504     644

  Fragments arranged by size: 

                1454     961     663     644     504     232
 
AfeI AGC'GCT

Cuts at:      0     638    4458 
   Size:        638    3820
 
AflIII A'CryG_T

Cuts at:      0    2672    3143    4458 
   Size:       2672     471    1315

  Fragments arranged by size: 

                2672    1315     471
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1362    2802    3255    3432    4458 
   Size:       1362    1440     453     177    1026

  Fragments arranged by size: 

                1440    1362    1026     453     177
 
AleI CACnn'nnGTG

Cuts at:      0    4340    4458 
   Size:       4340     118
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2930    4358    4458 
   Size:       2930    1428     100

  Fragments arranged by size: 

                2930    1428     100
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4438    4458 
   Size:       4438      20
 
AlwNI CAG_nnn'CTG

Cuts at:      0     978    1142    1386    1558    1698    1845    2130    2980 
   Size:        978     164     244     172     140     147     285     850

Cuts at:   2980    3204    3467    3486    4458 
   Size:        224     263      19     972

  Fragments arranged by size: 

                 978     972     850     285     263     244     224     172
                 164     147     140      19
 
ApaI G_GGCC'C

Cuts at:      0     467     851     889    1396    1637    2223    2260    3841 
   Size:        467     384      38     507     241     586      37    1581

Cuts at:   3841    4043    4458 
   Size:        202     415

  Fragments arranged by size: 

                1581     586     507     467     415     384     241     202
                  38      37
 
ApoI r'AATT_y

Cuts at:      0     151    1219    4458 
   Size:        151    1068    3239

  Fragments arranged by size: 

                3239    1068     151
 
AseI AT'TA_AT

Cuts at:      0     286    4458 
   Size:        286    4172
 
AvaI C'yCGr_G

Cuts at:      0     415     502     882    1375    1636    1911    2500    2653 
   Size:        415      87     380     493     261     275     589     153

Cuts at:   2653    3133    3512    3529    4036    4458 
   Size:        480     379      17     507     422

  Fragments arranged by size: 

                 589     507     493     480     422     415     380     379
                 275     261     153      87      17
 
BamHI G'GATC_C

Cuts at:      0    4057    4458 
   Size:       4057     401
 
BanI G'GyrC_C

Cuts at:      0    1537    2148    2166    2268    2914    3525    4276    4458 
   Size:       1537     611      18     102     646     611     751     182

  Fragments arranged by size: 

                1537     751     646     611     611     182     102      18
            
 
BanII G_rGCy'C

Cuts at:      0     422     467     851     889     967    1396    1604    1637 
   Size:        422      45     384      38      78     429     208      33

Cuts at:   1637    1759    1823    2009    2223    2260    2555    2702    2852 
   Size:        122      64     186     214      37     295     147     150

Cuts at:   2852    2992    3241    3599    3781    3841    4043    4458 
   Size:        140     249     358     182      60     202     415

  Fragments arranged by size: 

                 429     422     415     384     358     295     249     214
                 208     202     186     182     150     147     140     122
                  78      64      60      45      38      37      33
 
BbeI G_GCGC'C

Cuts at:      0    1541    2152    2272    4280    4458 
   Size:       1541     611     120    2008     178

  Fragments arranged by size: 

                2008    1541     611     178     120
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1181    1268    1361    4458 
   Size:       1181      87      93    3097

  Fragments arranged by size: 

                3097    1181      93      87
 
BbvCI CC'TCA_GC

Cuts at:      0     424     758    1325    2561    3355    3920    4248    4458 
   Size:        424     334     567    1236     794     565     328     210

  Fragments arranged by size: 

                1236     794     567     565     424     334     328     210
            
 
BclI T'GATC_A

Cuts at:      0     722    4458 
   Size:        722    3736
 
BglI GCCn_nnn'nGGC

Cuts at:      0     462     494     907    1567    2923    4458 
   Size:        462      32     413     660    1356    1535

  Fragments arranged by size: 

                1535    1356     660     462     413      32
 
BlpI GC'TnA_GC

Cuts at:      0    1105    2029    2386    3283    4458 
   Size:       1105     924     357     897    1175

  Fragments arranged by size: 

                1175    1105     924     897     357
 
Bme1580I G_kGCm'C

Cuts at:      0     467     851     889    1303    1396    1637    2171    2223 
   Size:        467     384      38     414      93     241     534      52

Cuts at:   2223    2260    2498    3460    3841    4043    4458 
   Size:         37     238     962     381     202     415

  Fragments arranged by size: 

                 962     534     467     415     414     384     381     241
                 238     202      93      52      38      37
 
BmgBI CAC'GTC

Cuts at:      0     948    4458 
   Size:        948    3510
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     362     607     845    1068    1306    1597    2373    4334 
   Size:        362     245     238     223     238     291     776    1961

Cuts at:   4334    4458 
   Size:        124

  Fragments arranged by size: 

                1961     776     362     291     245     238     238     223
                 124
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1953    3006    3069    3618    4458 
   Size:       1953    1053      63     549     840

  Fragments arranged by size: 

                1953    1053     840     549      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     757     916     943    1000    1033    1051    1119 
   Size:         43     714     159      27      57      33      18      68

Cuts at:   1119    1249    2110    2155    2716    3037    3039    3394    3844 
   Size:        130     861      45     561     321       2     355     450

Cuts at:   3844    4192    4346    4458 
   Size:        348     154     112

  Fragments arranged by size: 

                 861     714     561     450     355     348     321     159
                 154     130     112      68      57      45      43      33
                  27      18       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     424     758    1325    1580    1598    1873    2561    3355 
   Size:        424     334     567     255      18     275     688     794

Cuts at:   3355    3487    3920    4248    4458 
   Size:        132     433     328     210

  Fragments arranged by size: 

                 794     688     567     433     424     334     328     275
                 255     210     132      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2539    3604    4458 
   Size:       2539    1065     854

  Fragments arranged by size: 

                2539    1065     854
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     380    4458 
   Size:        380    4078
 
BsaAI yAC'GTr

Cuts at:      0    1737    2613    3146    4458 
   Size:       1737     876     533    1312

  Fragments arranged by size: 

                1737    1312     876     533
 
BsaHI Gr'CG_yC

Cuts at:      0     451    1538    1946    2072    2149    2269    2342    4277 
   Size:        451    1087     408     126      77     120      73    1935

Cuts at:   4277    4458 
   Size:        181

  Fragments arranged by size: 

                1935    1087     451     408     181     126     120      77
                  73
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      41      71    1972    2002    2353    2383    3133    3163 
   Size:         41      30    1901      30     351      30     750      30

Cuts at:   3163    4438    4458 
   Size:       1275      20

  Fragments arranged by size: 

                1901    1275     750     351      41      30      30      30
                  30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     412     625     703     997    1660    1663    2257 
   Size:        121     291     213      78     294     663       3     594

Cuts at:   2257    2415    2418    2421    2424    3051    3547    3589    3859 
   Size:        158       3       3       3     627     496      42     270

Cuts at:   3859    3925    4003    4194    4233    4236    4450    4458 
   Size:         66      78     191      39       3     214       8

  Fragments arranged by size: 

                 663     627     594     496     294     291     270     214
                 213     191     158     121      78      78      66      42
                  39       8       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     355     767    1237    1369    1389    1396    1444    1479 
   Size:        355     412     470     132      20       7      48      35

Cuts at:   1479    1524    1740    2769    2965    3460    4458 
   Size:         45     216    1029     196     495     998

  Fragments arranged by size: 

                1029     998     495     470     412     355     216     196
                 132      48      45      35      20       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     970    2323    4458 
   Size:         80     890    1353    2135

  Fragments arranged by size: 

                2135    1353     890      80
 
BsiEI CG_ry'CG

Cuts at:      0    2376    2463    2576    2812    4199    4458 
   Size:       2376      87     113     236    1387     259

  Fragments arranged by size: 

                2376    1387     259     236     113      87
 
BsiHKAI G_wGCw'C

Cuts at:      0     422     883     967    2555    2702    2992    3599    4458 
   Size:        422     461      84    1588     147     290     607     859

  Fragments arranged by size: 

                1588     859     607     461     422     290     147      84
            
 
BsmI GAATG_Cn'

Cuts at:      0     497    1958    4458 
   Size:        497    1461    2500

  Fragments arranged by size: 

                2500    1461     497
 
Bsp1286I G_dGCh'C

Cuts at:      0     422     467     851     883     889     967    1303    1396 
   Size:        422      45     384      32       6      78     336      93

Cuts at:   1396    1604    1637    1759    1823    2009    2171    2223    2260 
   Size:        208      33     122      64     186     162      52      37

Cuts at:   2260    2498    2555    2702    2852    2992    3241    3460    3599 
   Size:        238      57     147     150     140     249     219     139

Cuts at:   3599    3781    3841    4043    4458 
   Size:        182      60     202     415

  Fragments arranged by size: 

                 422     415     384     336     249     238     219     208
                 202     186     182     162     150     147     140     139
                 122      93      78      64      60      57      52      45
                  37      33      32       6
 
BspHI T'CATG_A

Cuts at:      0     725    4217    4458 
   Size:        725    3492     241

  Fragments arranged by size: 

                3492     725     241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1150    3260    4458 
   Size:       1150    2110    1198

  Fragments arranged by size: 

                2110    1198    1150
 
BsrBI CCG'CTC

Cuts at:      0    1017    1627    2162    2187    2274    3053    3071    3449 
   Size:       1017     610     535      25      87     779      18     378

Cuts at:   3449    4419    4458 
   Size:        970      39

  Fragments arranged by size: 

                1017     970     779     610     535     378      87      39
                  25      18
 
BsrFI r'CCGG_y

Cuts at:      0    2344    3418    4458 
   Size:       2344    1074    1040

  Fragments arranged by size: 

                2344    1074    1040
 
BsrGI T'GTAC_A

Cuts at:      0    2669    4458 
   Size:       2669    1789
 
BssHII G'CGCG_C

Cuts at:      0    2585    4458 
   Size:       2585    1873
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1386    1737    2130    4458 
   Size:       1386     351     393    2328

  Fragments arranged by size: 

                2328    1386     393     351
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     825    1128    1245    2911    4458 
   Size:        825     303     117    1666    1547

  Fragments arranged by size: 

                1666    1547     825     303     117
 
BstYI r'GATC_y

Cuts at:      0     926    1769    2750    4057    4458 
   Size:        926     843     981    1307     401

  Fragments arranged by size: 

                1307     981     926     843     401
 
Bsu36I CC'TnA_GG

Cuts at:      0     170    1256    3842    4458 
   Size:        170    1086    2586     616

  Fragments arranged by size: 

                2586    1086     616     170
 
BtgI C'CryG_G

Cuts at:      0    1604    2376    2475    2818    4458 
   Size:       1604     772      99     343    1640

  Fragments arranged by size: 

                1640    1604     772     343      99
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2019    4458 
   Size:       2019    2439
 
BtsI GCAGTG_nn'

Cuts at:      0     967    1180    2903    3544    4310    4458 
   Size:        967     213    1723     641     766     148

  Fragments arranged by size: 

                1723     967     766     641     213     148
 
ClaI AT'CG_AT

Cuts at:      0      19    4458 
   Size:         19    4439
 
DraIII CAC_nnn'GTG

Cuts at:      0     401     878    2104    4458 
   Size:        401     477    1226    2354

  Fragments arranged by size: 

                2354    1226     477     401
 
EaeI y'GGCC_r

Cuts at:      0       2     590    1028    1163    1247    2188    2198    2448 
   Size:          2     588     438     135      84     941      10     250

Cuts at:   2448    2460    2573    2726    2809    2889    3122    3440    4196 
   Size:         12     113     153      83      80     233     318     756

Cuts at:   4196    4385    4458 
   Size:        189      73

  Fragments arranged by size: 

                 941     756     588     438     318     250     233     189
                 153     135     113      84      83      80      73      12
                  10       2
 
EagI C'GGCC_G

Cuts at:      0    2460    2573    2809    4196    4458 
   Size:       2460     113     236    1387     262

  Fragments arranged by size: 

                2460    1387     262     236     113
 
EarI CTCTTCn'nnn_

Cuts at:      0     400    1052    2980    3920    4458 
   Size:        400     652    1928     940     538

  Fragments arranged by size: 

                1928     940     652     538     400
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     133     268     562     690     811     981    1637    2539 
   Size:        133     135     294     128     121     170     656     902

Cuts at:   2539    4458 
   Size:       1919

  Fragments arranged by size: 

                1919     902     656     294     170     135     133     128
                 121
 
EcoICRI GAG'CTC

Cuts at:      0     420     965    2553    2700    2990    3597    4458 
   Size:        420     545    1588     147     290     607     861

  Fragments arranged by size: 

                1588     861     607     545     420     290     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     757     916     943     961    1000    1033    1051 
   Size:         43     714     159      27      18      39      33      18

Cuts at:   1051    1119    1193    1249    2110    2155    2647    2716    3037 
   Size:         68      74      56     861      45     492      69     321

Cuts at:   3037    3039    3310    3394    3814    3844    4192    4346    4458 
   Size:          2     271      84     420      30     348     154     112

  Fragments arranged by size: 

                 861     714     492     420     348     321     271     159
                 154     112      84      74      69      68      56      45
                  43      39      33      30      27      18      18       2
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1153    1343    1931    2092    2779    4458 
   Size:       1153     190     588     161     687    1679

  Fragments arranged by size: 

                1679    1153     687     588     190     161
 
EcoO109I rG'GnC_Cy

Cuts at:      0     561     847     886    1393    1405    1633    2219    2220 
   Size:        561     286      39     507      12     228     586       1

Cuts at:   2220    2256    2257    2487    2945    3096    3174    3483    3659 
   Size:         36       1     230     458     151      78     309     176

Cuts at:   3659    3692    3727    3837    3838    3976    4040    4440    4458 
   Size:         33      35     110       1     138      64     400      18

  Fragments arranged by size: 

                 586     561     507     458     400     309     286     230
                 228     176     151     138     110      78      64      39
                  36      35      33      18      12       1       1       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     885    1218    1839    4458 
   Size:        885     333     621    2619

  Fragments arranged by size: 

                2619     885     621     333
 
HaeII r_GCGC'y

Cuts at:      0     640    1541    1873    2131    2152    2272    4280    4458 
   Size:        640     901     332     258      21     120    2008     178

  Fragments arranged by size: 

                2008     901     640     332     258     178     120      21
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     436     468     682     714     973    1005 
   Size:        177      32     227      32     214      32     259      32

Cuts at:   1005    1348    1380    1889    1921    1972    2004    2176    2208 
   Size:        343      32     509      32      51      32     172      32

Cuts at:   2208    2788    2820    3133    3165    3241    3273    3418    3450 
   Size:        580      32     313      32      76      32     145      32

Cuts at:   3450    4060    4092    4374    4406    4458 
   Size:        610      32     282      32      52

  Fragments arranged by size: 

                 610     580     509     343     313     282     259     227
                 214     177     172     145      76      52      51      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     810    4458 
   Size:        810    3648
 
HindIII A'AGCT_T

Cuts at:      0     207    4458 
   Size:        207    4251
 
KasI G'GCGC_C

Cuts at:      0    1537    2148    2268    4276    4458 
   Size:       1537     611     120    2008     182

  Fragments arranged by size: 

                2008    1537     611     182     120
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1829    2396    4458 
   Size:       1829     567    2062

  Fragments arranged by size: 

                2062    1829     567
 
MscI TGG'CCA

Cuts at:      0    1165    1249    2200    2450    2728    3124    4387    4458 
   Size:       1165      84     951     250     278     396    1263      71

  Fragments arranged by size: 

                1263    1165     951     396     278     250      84      71
            
 
MslI CAynn'nnrTG

Cuts at:      0    1831    1885    2860    4340    4458 
   Size:       1831      54     975    1480     118

  Fragments arranged by size: 

                1831    1480     975     118      54
 
MspA1I CmG'CkG

Cuts at:      0     771     798    1606    1656    1695    1719    2028    2252 
   Size:        771      27     808      50      39      24     309     224

Cuts at:   2252    2378    2477    2646    2868    3201    3321    3564    4368 
   Size:        126      99     169     222     333     120     243     804

Cuts at:   4368    4458 
   Size:         90

  Fragments arranged by size: 

                 808     804     771     333     309     243     224     222
                 169     126     120      99      90      50      39      27
                  24
 
NaeI GCC'GGC

Cuts at:      0    2346    4458 
   Size:       2346    2112
 
NarI GG'CG_CC

Cuts at:      0    1538    2149    2269    4277    4458 
   Size:       1538     611     120    2008     181

  Fragments arranged by size: 

                2008    1538     611     181     120
 
NcoI C'CATG_G

Cuts at:      0    2818    4458 
   Size:       2818    1640
 
NgoMIV G'CCGG_C

Cuts at:      0    2344    4458 
   Size:       2344    2114
 
NspI r_CATG'y

Cuts at:      0    2676    2802    2865    3318    4458 
   Size:       2676     126      63     453    1140

  Fragments arranged by size: 

                2676    1140     453     126      63
 
PasI CC'CwG_GG

Cuts at:      0     843    1155    2153    3100    4458 
   Size:        843     312     998     947    1358

  Fragments arranged by size: 

                1358     998     947     843     312
 
PciI A'CATG_T

Cuts at:      0    2672    4458 
   Size:       2672    1786
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1161    1790    2898    3272    3345    4458 
   Size:       1161     629    1108     374      73    1113

  Fragments arranged by size: 

                1161    1113    1108     629     374      73
 
PfoI T'CCnGG_A

Cuts at:      0     788    2087    3110    3661    3729    4458 
   Size:        788    1299    1023     551      68     729

  Fragments arranged by size: 

                1299    1023     788     729     551      68
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4177    4209    4438    4458 
   Size:       4177      32     229      20

  Fragments arranged by size: 

                4177     229      32      20
 
PpuMI rG'GwC_Cy

Cuts at:      0    2945    3174    3483    3659    3692    3727    3976    4458 
   Size:       2945     229     309     176      33      35     249     482

  Fragments arranged by size: 

                2945     482     309     249     229     176      35      33
            
 
PspOMI G'GGCC_C

Cuts at:      0     463     847     885    1392    1633    2219    2256    3837 
   Size:        463     384      38     507     241     586      37    1581

Cuts at:   3837    4039    4458 
   Size:        202     419

  Fragments arranged by size: 

                1581     586     507     463     419     384     241     202
                  38      37
 
PstI C_TGCA'G

Cuts at:      0     350     536     974    1147    3476    3500    4168    4458 
   Size:        350     186     438     173    2329      24     668     290

  Fragments arranged by size: 

                2329     668     438     350     290     186     173      24
            
 
PvuII CAG'CTG

Cuts at:      0     798    1656    1695    2868    3321    3564    4458 
   Size:        798     858      39    1173     453     243     894

  Fragments arranged by size: 

                1173     894     858     798     453     243      39
 
SacI G_AGCT'C

Cuts at:      0     422     967    2555    2702    2992    3599    4458 
   Size:        422     545    1588     147     290     607     859

  Fragments arranged by size: 

                1588     859     607     545     422     290     147
 
SacII CC_GC'GG

Cuts at:      0    1607    2379    2478    4458 
   Size:       1607     772      99    1980

  Fragments arranged by size: 

                1980    1607     772      99
 
SanDI GG'GwC_CC

Cuts at:      0    3692    4458 
   Size:       3692     766
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2980    4458 
   Size:       2980    1478
 
SfcI C'TryA_G

Cuts at:      0     346     532     970    1143    3472    3496    3654    4164 
   Size:        346     186     438     173    2329      24     158     510

Cuts at:   4164    4458 
   Size:        294

  Fragments arranged by size: 

                2329     510     438     346     294     186     173     158
                  24
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     907    4458 
   Size:        907    3551
 
SfoI GGC'GCC

Cuts at:      0    1539    2150    2270    4278    4458 
   Size:       1539     611     120    2008     180

  Fragments arranged by size: 

                2008    1539     611     180     120
 
SmaI CCC'GGG

Cuts at:      0     504    1377    1638    3514    4038    4458 
   Size:        504     873     261    1876     524     420

  Fragments arranged by size: 

                1876     873     524     504     420     261
 
SmlI C'TyrA_G

Cuts at:      0    2554    3619    4458 
   Size:       2554    1065     839

  Fragments arranged by size: 

                2554    1065     839
 
SphI G_CATG'C

Cuts at:      0    2865    3318    4458 
   Size:       2865     453    1140

  Fragments arranged by size: 

                2865    1140     453
 
SspI AAT'ATT

Cuts at:      0      31    4458 
   Size:         31    4427
 
StuI AGG'CCT

Cuts at:      0    1201    3556    4214    4458 
   Size:       1201    2355     658     244

  Fragments arranged by size: 

                2355    1201     658     244
 
StyI C'CwwG_G

Cuts at:      0     701     914    2228    2818    2941    3972    4346    4458 
   Size:        701     213    1314     590     123    1031     374     112

  Fragments arranged by size: 

                1314    1031     701     590     374     213     123     112
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     490    1749    3773    3883    4458 
   Size:        490    1259    2024     110     575

  Fragments arranged by size: 

                2024    1259     575     490     110
 
TatI w'GTAC_w

Cuts at:      0     573    1863    2669    3080    4458 
   Size:        573    1290     806     411    1378

  Fragments arranged by size: 

                1378    1290     806     573     411
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2353    2385    4458 
   Size:       2353      32    2073

  Fragments arranged by size: 

                2353    2073      32
 
XbaI T'CTAG_A

Cuts at:      0    1962    4458 
   Size:       1962    2496
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     850    1050    2016    4317    4458 
   Size:        850     200     966    2301     141

  Fragments arranged by size: 

                2301     966     850     200     141
 
XmaI C'CCGG_G

Cuts at:      0     502    1375    1636    3512    4036    4458 
   Size:        502     873     261    1876     524     422

  Fragments arranged by size: 

                1876     873     524     502     422     261
 
XmnI GAAnn'nnTTC

Cuts at:      0    1840    4458 
   Size:       1840    2618
 
ZraI GAC'GTC

Cuts at:      0     452    4458 
   Size:        452    4006
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI
    PvuII     SacI    SacII    SanDI     SapI     SfcI     SfiI     SfoI
     SmaI     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI    ApaLI     AscI    AsiSI    AvrII
     BaeI     BcgI    BciVI    BfrBI    BglII     BmtI    BsaBI    BsaWI
    BsiWI    BsmBI    BspEI    BsrDI    BssSI    BstBI   BstEII  BstZ17I
    CspCI     DraI     DrdI    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SalI     SbfI     ScaI    SexAI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SwaI  Tth111I     XhoI
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