(Linear) (Six-base) MAPSORT of: KIAA0293.seq Check: 2096 from: 1 to: 4458 KIAA0293.orf With 182 enzymes: SgfI * October 24, 2006 15:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1150 4458 Size: 1150 3308 AatII G_ACGT'C Cuts at: 0 454 4458 Size: 454 4004 AccI GT'mk_AC Cuts at: 0 2687 4458 Size: 2687 1771 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 961 1193 2647 3310 3814 4458 Size: 961 232 1454 663 504 644 Fragments arranged by size: 1454 961 663 644 504 232 AfeI AGC'GCT Cuts at: 0 638 4458 Size: 638 3820 AflIII A'CryG_T Cuts at: 0 2672 3143 4458 Size: 2672 471 1315 Fragments arranged by size: 2672 1315 471 AhdI GACnn_n'nnGTC Cuts at: 0 1362 2802 3255 3432 4458 Size: 1362 1440 453 177 1026 Fragments arranged by size: 1440 1362 1026 453 177 AleI CACnn'nnGTG Cuts at: 0 4340 4458 Size: 4340 118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2930 4358 4458 Size: 2930 1428 100 Fragments arranged by size: 2930 1428 100 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4438 4458 Size: 4438 20 AlwNI CAG_nnn'CTG Cuts at: 0 978 1142 1386 1558 1698 1845 2130 2980 Size: 978 164 244 172 140 147 285 850 Cuts at: 2980 3204 3467 3486 4458 Size: 224 263 19 972 Fragments arranged by size: 978 972 850 285 263 244 224 172 164 147 140 19 ApaI G_GGCC'C Cuts at: 0 467 851 889 1396 1637 2223 2260 3841 Size: 467 384 38 507 241 586 37 1581 Cuts at: 3841 4043 4458 Size: 202 415 Fragments arranged by size: 1581 586 507 467 415 384 241 202 38 37 ApoI r'AATT_y Cuts at: 0 151 1219 4458 Size: 151 1068 3239 Fragments arranged by size: 3239 1068 151 AseI AT'TA_AT Cuts at: 0 286 4458 Size: 286 4172 AvaI C'yCGr_G Cuts at: 0 415 502 882 1375 1636 1911 2500 2653 Size: 415 87 380 493 261 275 589 153 Cuts at: 2653 3133 3512 3529 4036 4458 Size: 480 379 17 507 422 Fragments arranged by size: 589 507 493 480 422 415 380 379 275 261 153 87 17 BamHI G'GATC_C Cuts at: 0 4057 4458 Size: 4057 401 BanI G'GyrC_C Cuts at: 0 1537 2148 2166 2268 2914 3525 4276 4458 Size: 1537 611 18 102 646 611 751 182 Fragments arranged by size: 1537 751 646 611 611 182 102 18 BanII G_rGCy'C Cuts at: 0 422 467 851 889 967 1396 1604 1637 Size: 422 45 384 38 78 429 208 33 Cuts at: 1637 1759 1823 2009 2223 2260 2555 2702 2852 Size: 122 64 186 214 37 295 147 150 Cuts at: 2852 2992 3241 3599 3781 3841 4043 4458 Size: 140 249 358 182 60 202 415 Fragments arranged by size: 429 422 415 384 358 295 249 214 208 202 186 182 150 147 140 122 78 64 60 45 38 37 33 BbeI G_GCGC'C Cuts at: 0 1541 2152 2272 4280 4458 Size: 1541 611 120 2008 178 Fragments arranged by size: 2008 1541 611 178 120 BbsI GAAGACnn'nnnn_ Cuts at: 0 1181 1268 1361 4458 Size: 1181 87 93 3097 Fragments arranged by size: 3097 1181 93 87 BbvCI CC'TCA_GC Cuts at: 0 424 758 1325 2561 3355 3920 4248 4458 Size: 424 334 567 1236 794 565 328 210 Fragments arranged by size: 1236 794 567 565 424 334 328 210 BclI T'GATC_A Cuts at: 0 722 4458 Size: 722 3736 BglI GCCn_nnn'nGGC Cuts at: 0 462 494 907 1567 2923 4458 Size: 462 32 413 660 1356 1535 Fragments arranged by size: 1535 1356 660 462 413 32 BlpI GC'TnA_GC Cuts at: 0 1105 2029 2386 3283 4458 Size: 1105 924 357 897 1175 Fragments arranged by size: 1175 1105 924 897 357 Bme1580I G_kGCm'C Cuts at: 0 467 851 889 1303 1396 1637 2171 2223 Size: 467 384 38 414 93 241 534 52 Cuts at: 2223 2260 2498 3460 3841 4043 4458 Size: 37 238 962 381 202 415 Fragments arranged by size: 962 534 467 415 414 384 381 241 238 202 93 52 38 37 BmgBI CAC'GTC Cuts at: 0 948 4458 Size: 948 3510 BmrI ACTGGGnnnn_n' Cuts at: 0 362 607 845 1068 1306 1597 2373 4334 Size: 362 245 238 223 238 291 776 1961 Cuts at: 4334 4458 Size: 124 Fragments arranged by size: 1961 776 362 291 245 238 238 223 124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1953 3006 3069 3618 4458 Size: 1953 1053 63 549 840 Fragments arranged by size: 1953 1053 840 549 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 757 916 943 1000 1033 1051 1119 Size: 43 714 159 27 57 33 18 68 Cuts at: 1119 1249 2110 2155 2716 3037 3039 3394 3844 Size: 130 861 45 561 321 2 355 450 Cuts at: 3844 4192 4346 4458 Size: 348 154 112 Fragments arranged by size: 861 714 561 450 355 348 321 159 154 130 112 68 57 45 43 33 27 18 2 Bpu10I CC'TnA_GC Cuts at: 0 424 758 1325 1580 1598 1873 2561 3355 Size: 424 334 567 255 18 275 688 794 Cuts at: 3355 3487 3920 4248 4458 Size: 132 433 328 210 Fragments arranged by size: 794 688 567 433 424 334 328 275 255 210 132 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2539 3604 4458 Size: 2539 1065 854 Fragments arranged by size: 2539 1065 854 BsaI GGTCTCn'nnnn_ Cuts at: 0 380 4458 Size: 380 4078 BsaAI yAC'GTr Cuts at: 0 1737 2613 3146 4458 Size: 1737 876 533 1312 Fragments arranged by size: 1737 1312 876 533 BsaHI Gr'CG_yC Cuts at: 0 451 1538 1946 2072 2149 2269 2342 4277 Size: 451 1087 408 126 77 120 73 1935 Cuts at: 4277 4458 Size: 181 Fragments arranged by size: 1935 1087 451 408 181 126 120 77 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 41 71 1972 2002 2353 2383 3133 3163 Size: 41 30 1901 30 351 30 750 30 Cuts at: 3163 4438 4458 Size: 1275 20 Fragments arranged by size: 1901 1275 750 351 41 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 121 412 625 703 997 1660 1663 2257 Size: 121 291 213 78 294 663 3 594 Cuts at: 2257 2415 2418 2421 2424 3051 3547 3589 3859 Size: 158 3 3 3 627 496 42 270 Cuts at: 3859 3925 4003 4194 4233 4236 4450 4458 Size: 66 78 191 39 3 214 8 Fragments arranged by size: 663 627 594 496 294 291 270 214 213 191 158 121 78 78 66 42 39 8 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 355 767 1237 1369 1389 1396 1444 1479 Size: 355 412 470 132 20 7 48 35 Cuts at: 1479 1524 1740 2769 2965 3460 4458 Size: 45 216 1029 196 495 998 Fragments arranged by size: 1029 998 495 470 412 355 216 196 132 48 45 35 20 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 970 2323 4458 Size: 80 890 1353 2135 Fragments arranged by size: 2135 1353 890 80 BsiEI CG_ry'CG Cuts at: 0 2376 2463 2576 2812 4199 4458 Size: 2376 87 113 236 1387 259 Fragments arranged by size: 2376 1387 259 236 113 87 BsiHKAI G_wGCw'C Cuts at: 0 422 883 967 2555 2702 2992 3599 4458 Size: 422 461 84 1588 147 290 607 859 Fragments arranged by size: 1588 859 607 461 422 290 147 84 BsmI GAATG_Cn' Cuts at: 0 497 1958 4458 Size: 497 1461 2500 Fragments arranged by size: 2500 1461 497 Bsp1286I G_dGCh'C Cuts at: 0 422 467 851 883 889 967 1303 1396 Size: 422 45 384 32 6 78 336 93 Cuts at: 1396 1604 1637 1759 1823 2009 2171 2223 2260 Size: 208 33 122 64 186 162 52 37 Cuts at: 2260 2498 2555 2702 2852 2992 3241 3460 3599 Size: 238 57 147 150 140 249 219 139 Cuts at: 3599 3781 3841 4043 4458 Size: 182 60 202 415 Fragments arranged by size: 422 415 384 336 249 238 219 208 202 186 182 162 150 147 140 139 122 93 78 64 60 57 52 45 37 33 32 6 BspHI T'CATG_A Cuts at: 0 725 4217 4458 Size: 725 3492 241 Fragments arranged by size: 3492 725 241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1150 3260 4458 Size: 1150 2110 1198 Fragments arranged by size: 2110 1198 1150 BsrBI CCG'CTC Cuts at: 0 1017 1627 2162 2187 2274 3053 3071 3449 Size: 1017 610 535 25 87 779 18 378 Cuts at: 3449 4419 4458 Size: 970 39 Fragments arranged by size: 1017 970 779 610 535 378 87 39 25 18 BsrFI r'CCGG_y Cuts at: 0 2344 3418 4458 Size: 2344 1074 1040 Fragments arranged by size: 2344 1074 1040 BsrGI T'GTAC_A Cuts at: 0 2669 4458 Size: 2669 1789 BssHII G'CGCG_C Cuts at: 0 2585 4458 Size: 2585 1873 BstAPI GCAn_nnn'nTGC Cuts at: 0 1386 1737 2130 4458 Size: 1386 351 393 2328 Fragments arranged by size: 2328 1386 393 351 BstXI CCAn_nnnn'nTGG Cuts at: 0 825 1128 1245 2911 4458 Size: 825 303 117 1666 1547 Fragments arranged by size: 1666 1547 825 303 117 BstYI r'GATC_y Cuts at: 0 926 1769 2750 4057 4458 Size: 926 843 981 1307 401 Fragments arranged by size: 1307 981 926 843 401 Bsu36I CC'TnA_GG Cuts at: 0 170 1256 3842 4458 Size: 170 1086 2586 616 Fragments arranged by size: 2586 1086 616 170 BtgI C'CryG_G Cuts at: 0 1604 2376 2475 2818 4458 Size: 1604 772 99 343 1640 Fragments arranged by size: 1640 1604 772 343 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2019 4458 Size: 2019 2439 BtsI GCAGTG_nn' Cuts at: 0 967 1180 2903 3544 4310 4458 Size: 967 213 1723 641 766 148 Fragments arranged by size: 1723 967 766 641 213 148 ClaI AT'CG_AT Cuts at: 0 19 4458 Size: 19 4439 DraIII CAC_nnn'GTG Cuts at: 0 401 878 2104 4458 Size: 401 477 1226 2354 Fragments arranged by size: 2354 1226 477 401 EaeI y'GGCC_r Cuts at: 0 2 590 1028 1163 1247 2188 2198 2448 Size: 2 588 438 135 84 941 10 250 Cuts at: 2448 2460 2573 2726 2809 2889 3122 3440 4196 Size: 12 113 153 83 80 233 318 756 Cuts at: 4196 4385 4458 Size: 189 73 Fragments arranged by size: 941 756 588 438 318 250 233 189 153 135 113 84 83 80 73 12 10 2 EagI C'GGCC_G Cuts at: 0 2460 2573 2809 4196 4458 Size: 2460 113 236 1387 262 Fragments arranged by size: 2460 1387 262 236 113 EarI CTCTTCn'nnn_ Cuts at: 0 400 1052 2980 3920 4458 Size: 400 652 1928 940 538 Fragments arranged by size: 1928 940 652 538 400 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 133 268 562 690 811 981 1637 2539 Size: 133 135 294 128 121 170 656 902 Cuts at: 2539 4458 Size: 1919 Fragments arranged by size: 1919 902 656 294 170 135 133 128 121 EcoICRI GAG'CTC Cuts at: 0 420 965 2553 2700 2990 3597 4458 Size: 420 545 1588 147 290 607 861 Fragments arranged by size: 1588 861 607 545 420 290 147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 757 916 943 961 1000 1033 1051 Size: 43 714 159 27 18 39 33 18 Cuts at: 1051 1119 1193 1249 2110 2155 2647 2716 3037 Size: 68 74 56 861 45 492 69 321 Cuts at: 3037 3039 3310 3394 3814 3844 4192 4346 4458 Size: 2 271 84 420 30 348 154 112 Fragments arranged by size: 861 714 492 420 348 321 271 159 154 112 84 74 69 68 56 45 43 39 33 30 27 18 18 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 1153 1343 1931 2092 2779 4458 Size: 1153 190 588 161 687 1679 Fragments arranged by size: 1679 1153 687 588 190 161 EcoO109I rG'GnC_Cy Cuts at: 0 561 847 886 1393 1405 1633 2219 2220 Size: 561 286 39 507 12 228 586 1 Cuts at: 2220 2256 2257 2487 2945 3096 3174 3483 3659 Size: 36 1 230 458 151 78 309 176 Cuts at: 3659 3692 3727 3837 3838 3976 4040 4440 4458 Size: 33 35 110 1 138 64 400 18 Fragments arranged by size: 586 561 507 458 400 309 286 230 228 176 151 138 110 78 64 39 36 35 33 18 12 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 885 1218 1839 4458 Size: 885 333 621 2619 Fragments arranged by size: 2619 885 621 333 HaeII r_GCGC'y Cuts at: 0 640 1541 1873 2131 2152 2272 4280 4458 Size: 640 901 332 258 21 120 2008 178 Fragments arranged by size: 2008 901 640 332 258 178 120 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 177 209 436 468 682 714 973 1005 Size: 177 32 227 32 214 32 259 32 Cuts at: 1005 1348 1380 1889 1921 1972 2004 2176 2208 Size: 343 32 509 32 51 32 172 32 Cuts at: 2208 2788 2820 3133 3165 3241 3273 3418 3450 Size: 580 32 313 32 76 32 145 32 Cuts at: 3450 4060 4092 4374 4406 4458 Size: 610 32 282 32 52 Fragments arranged by size: 610 580 509 343 313 282 259 227 214 177 172 145 76 52 51 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 810 4458 Size: 810 3648 HindIII A'AGCT_T Cuts at: 0 207 4458 Size: 207 4251 KasI G'GCGC_C Cuts at: 0 1537 2148 2268 4276 4458 Size: 1537 611 120 2008 182 Fragments arranged by size: 2008 1537 611 182 120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1829 2396 4458 Size: 1829 567 2062 Fragments arranged by size: 2062 1829 567 MscI TGG'CCA Cuts at: 0 1165 1249 2200 2450 2728 3124 4387 4458 Size: 1165 84 951 250 278 396 1263 71 Fragments arranged by size: 1263 1165 951 396 278 250 84 71 MslI CAynn'nnrTG Cuts at: 0 1831 1885 2860 4340 4458 Size: 1831 54 975 1480 118 Fragments arranged by size: 1831 1480 975 118 54 MspA1I CmG'CkG Cuts at: 0 771 798 1606 1656 1695 1719 2028 2252 Size: 771 27 808 50 39 24 309 224 Cuts at: 2252 2378 2477 2646 2868 3201 3321 3564 4368 Size: 126 99 169 222 333 120 243 804 Cuts at: 4368 4458 Size: 90 Fragments arranged by size: 808 804 771 333 309 243 224 222 169 126 120 99 90 50 39 27 24 NaeI GCC'GGC Cuts at: 0 2346 4458 Size: 2346 2112 NarI GG'CG_CC Cuts at: 0 1538 2149 2269 4277 4458 Size: 1538 611 120 2008 181 Fragments arranged by size: 2008 1538 611 181 120 NcoI C'CATG_G Cuts at: 0 2818 4458 Size: 2818 1640 NgoMIV G'CCGG_C Cuts at: 0 2344 4458 Size: 2344 2114 NspI r_CATG'y Cuts at: 0 2676 2802 2865 3318 4458 Size: 2676 126 63 453 1140 Fragments arranged by size: 2676 1140 453 126 63 PasI CC'CwG_GG Cuts at: 0 843 1155 2153 3100 4458 Size: 843 312 998 947 1358 Fragments arranged by size: 1358 998 947 843 312 PciI A'CATG_T Cuts at: 0 2672 4458 Size: 2672 1786 PflMI CCAn_nnn'nTGG Cuts at: 0 1161 1790 2898 3272 3345 4458 Size: 1161 629 1108 374 73 1113 Fragments arranged by size: 1161 1113 1108 629 374 73 PfoI T'CCnGG_A Cuts at: 0 788 2087 3110 3661 3729 4458 Size: 788 1299 1023 551 68 729 Fragments arranged by size: 1299 1023 788 729 551 68 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4177 4209 4438 4458 Size: 4177 32 229 20 Fragments arranged by size: 4177 229 32 20 PpuMI rG'GwC_Cy Cuts at: 0 2945 3174 3483 3659 3692 3727 3976 4458 Size: 2945 229 309 176 33 35 249 482 Fragments arranged by size: 2945 482 309 249 229 176 35 33 PspOMI G'GGCC_C Cuts at: 0 463 847 885 1392 1633 2219 2256 3837 Size: 463 384 38 507 241 586 37 1581 Cuts at: 3837 4039 4458 Size: 202 419 Fragments arranged by size: 1581 586 507 463 419 384 241 202 38 37 PstI C_TGCA'G Cuts at: 0 350 536 974 1147 3476 3500 4168 4458 Size: 350 186 438 173 2329 24 668 290 Fragments arranged by size: 2329 668 438 350 290 186 173 24 PvuII CAG'CTG Cuts at: 0 798 1656 1695 2868 3321 3564 4458 Size: 798 858 39 1173 453 243 894 Fragments arranged by size: 1173 894 858 798 453 243 39 SacI G_AGCT'C Cuts at: 0 422 967 2555 2702 2992 3599 4458 Size: 422 545 1588 147 290 607 859 Fragments arranged by size: 1588 859 607 545 422 290 147 SacII CC_GC'GG Cuts at: 0 1607 2379 2478 4458 Size: 1607 772 99 1980 Fragments arranged by size: 1980 1607 772 99 SanDI GG'GwC_CC Cuts at: 0 3692 4458 Size: 3692 766 SapI GCTCTTCn'nnn_ Cuts at: 0 2980 4458 Size: 2980 1478 SfcI C'TryA_G Cuts at: 0 346 532 970 1143 3472 3496 3654 4164 Size: 346 186 438 173 2329 24 158 510 Cuts at: 4164 4458 Size: 294 Fragments arranged by size: 2329 510 438 346 294 186 173 158 24 SfiI GGCCn_nnn'nGGCC Cuts at: 0 907 4458 Size: 907 3551 SfoI GGC'GCC Cuts at: 0 1539 2150 2270 4278 4458 Size: 1539 611 120 2008 180 Fragments arranged by size: 2008 1539 611 180 120 SmaI CCC'GGG Cuts at: 0 504 1377 1638 3514 4038 4458 Size: 504 873 261 1876 524 420 Fragments arranged by size: 1876 873 524 504 420 261 SmlI C'TyrA_G Cuts at: 0 2554 3619 4458 Size: 2554 1065 839 Fragments arranged by size: 2554 1065 839 SphI G_CATG'C Cuts at: 0 2865 3318 4458 Size: 2865 453 1140 Fragments arranged by size: 2865 1140 453 SspI AAT'ATT Cuts at: 0 31 4458 Size: 31 4427 StuI AGG'CCT Cuts at: 0 1201 3556 4214 4458 Size: 1201 2355 658 244 Fragments arranged by size: 2355 1201 658 244 StyI C'CwwG_G Cuts at: 0 701 914 2228 2818 2941 3972 4346 4458 Size: 701 213 1314 590 123 1031 374 112 Fragments arranged by size: 1314 1031 701 590 374 213 123 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 490 1749 3773 3883 4458 Size: 490 1259 2024 110 575 Fragments arranged by size: 2024 1259 575 490 110 TatI w'GTAC_w Cuts at: 0 573 1863 2669 3080 4458 Size: 573 1290 806 411 1378 Fragments arranged by size: 1378 1290 806 573 411 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2353 2385 4458 Size: 2353 32 2073 Fragments arranged by size: 2353 2073 32 XbaI T'CTAG_A Cuts at: 0 1962 4458 Size: 1962 2496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 850 1050 2016 4317 4458 Size: 850 200 966 2301 141 Fragments arranged by size: 2301 966 850 200 141 XmaI C'CCGG_G Cuts at: 0 502 1375 1636 3512 4036 4458 Size: 502 873 261 1876 524 422 Fragments arranged by size: 1876 873 524 502 422 261 XmnI GAAnn'nnTTC Cuts at: 0 1840 4458 Size: 1840 2618 ZraI GAC'GTC Cuts at: 0 452 4458 Size: 452 4006 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SfcI SfiI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI ApaLI AscI AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BmtI BsaBI BsaWI BsiWI BsmBI BspEI BsrDI BssSI BstBI BstEII BstZ17I CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I XhoI