Restriction map for
pF1KA0294
(Linear) (Six-base) MAPSORT of: KIAA0294.seq Check: 2930 from: 1 to: 4032
KIAA0294.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3359 4032
Size: 3359 673
AccI GT'mk_AC
Cuts at: 0 1214 3141 4032
Size: 1214 1927 891
Fragments arranged by size:
1927 1214 891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1336 1678 1681 2032 2950 3874 4032
Size: 976 360 342 3 351 918 924 158
Fragments arranged by size:
976 924 918 360 351 342 158 3
AfeI AGC'GCT
Cuts at: 0 333 4032
Size: 333 3699
AflII C'TTAA_G
Cuts at: 0 1243 4032
Size: 1243 2789
AflIII A'CryG_T
Cuts at: 0 1519 2034 3158 4032
Size: 1519 515 1124 874
Fragments arranged by size:
1519 1124 874 515
AleI CACnn'nnGTG
Cuts at: 0 226 322 1498 4032
Size: 226 96 1176 2534
Fragments arranged by size:
2534 1176 226 96
AlwNI CAG_nnn'CTG
Cuts at: 0 670 3027 3408 4032
Size: 670 2357 381 624
Fragments arranged by size:
2357 670 624 381
ApaLI G'TGCA_C
Cuts at: 0 3952 4032
Size: 3952 80
ApoI r'AATT_y
Cuts at: 0 757 1104 1546 2354 2748 3562 4032
Size: 757 347 442 808 394 814 470
Fragments arranged by size:
814 808 757 470 442 394 347
AvaI C'yCGr_G
Cuts at: 0 1159 2371 2857 3634 4032
Size: 1159 1212 486 777 398
Fragments arranged by size:
1212 1159 777 486 398
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2155 2188 4032
Size: 2155 33 1844
Fragments arranged by size:
2155 1844 33
BamHI G'GATC_C
Cuts at: 0 3082 3858 4032
Size: 3082 776 174
Fragments arranged by size:
3082 776 174
BanI G'GyrC_C
Cuts at: 0 378 402 991 2087 2844 3398 3454 3990
Size: 378 24 589 1096 757 554 56 536
Cuts at: 3990 4032
Size: 42
Fragments arranged by size:
1096 757 589 554 536 378 56 42
24
BanII G_rGCy'C
Cuts at: 0 17 1718 3075 4032
Size: 17 1701 1357 957
Fragments arranged by size:
1701 1357 957 17
BbeI G_GCGC'C
Cuts at: 0 3994 4032
Size: 3994 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1324 1419 1914 2528 3161 3180 3656 4032
Size: 1324 95 495 614 633 19 476 376
Fragments arranged by size:
1324 633 614 495 476 376 95 19
BbvCI CC'TCA_GC
Cuts at: 0 335 4032
Size: 335 3697
BclI T'GATC_A
Cuts at: 0 1761 3110 4032
Size: 1761 1349 922
Fragments arranged by size:
1761 1349 922
BfrBI ATG'CAT
Cuts at: 0 1465 4032
Size: 1465 2567
BglI GCCn_nnn'nGGC
Cuts at: 0 586 3913 4032
Size: 586 3327 119
Fragments arranged by size:
3327 586 119
BglII A'GATC_T
Cuts at: 0 1081 4032
Size: 1081 2951
Bme1580I G_kGCm'C
Cuts at: 0 329 365 407 2847 3401 3956 4032
Size: 329 36 42 2440 554 555 76
Fragments arranged by size:
2440 555 554 329 76 42 36
BmgBI CAC'GTC
Cuts at: 0 464 3017 3493 4032
Size: 464 2553 476 539
Fragments arranged by size:
2553 539 476 464
BmrI ACTGGGnnnn_n'
Cuts at: 0 220 1210 2038 4032
Size: 220 990 828 1994
Fragments arranged by size:
1994 990 828 220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1419 3812 4032
Size: 1419 2393 220
Fragments arranged by size:
2393 1419 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 230 423 1027 1630 3250 3789 4032
Size: 185 45 193 604 603 1620 539 243
Fragments arranged by size:
1620 604 603 539 243 193 185 45
Bpu10I CC'TnA_GC
Cuts at: 0 335 937 3787 4032
Size: 335 602 2850 245
Fragments arranged by size:
2850 602 335 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1495 3328 4032
Size: 1111 384 1833 704
Fragments arranged by size:
1833 1111 704 384
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 2854 3537 4032
Size: 358 2496 683 495
Fragments arranged by size:
2496 683 495 358
BsaAI yAC'GTr
Cuts at: 0 1233 4032
Size: 1233 2799
BsaBI GATnn'nnATC
Cuts at: 0 1760 2531 2731 4032
Size: 1760 771 200 1301
Fragments arranged by size:
1760 1301 771 200
BsaHI Gr'CG_yC
Cuts at: 0 2042 3122 3281 3991 4032
Size: 2042 1080 159 710 41
Fragments arranged by size:
2042 1080 710 159 41
BsaWI w'CCGG_w
Cuts at: 0 3175 3517 4032
Size: 3175 342 515
Fragments arranged by size:
3175 515 342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 738 768 1883 1913 3170 3200 3458 3488
Size: 738 30 1115 30 1257 30 258 30
Cuts at: 3488 3754 3784 4032
Size: 266 30 248
Fragments arranged by size:
1257 1115 738 266 258 248 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 355 544 1904 2506 2551 2836 2926
Size: 352 3 189 1360 602 45 285 90
Cuts at: 2926 3692 3762 4032
Size: 766 70 270
Fragments arranged by size:
1360 766 602 352 285 270 189 90
70 45 3
BseYI C'CCAG_C
Cuts at: 0 328 584 3738 4032
Size: 328 256 3154 294
Fragments arranged by size:
3154 328 294 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 3571 4032
Size: 167 3404 461
Fragments arranged by size:
3404 461 167
BsiEI CG_ry'CG
Cuts at: 0 561 1581 2398 2870 3998 4032
Size: 561 1020 817 472 1128 34
Fragments arranged by size:
1128 1020 817 561 472 34
BsiHKAI G_wGCw'C
Cuts at: 0 254 875 1718 2081 3821 3956 4032
Size: 254 621 843 363 1740 135 76
Fragments arranged by size:
1740 843 621 363 254 135 76
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2742 3023 4032
Size: 2742 281 1009
Fragments arranged by size:
2742 1009 281
Bsp1286I G_dGCh'C
Cuts at: 0 17 254 329 365 407 875 1718 2081
Size: 17 237 75 36 42 468 843 363
Cuts at: 2081 2847 3075 3401 3821 3956 4032
Size: 766 228 326 420 135 76
Fragments arranged by size:
843 766 468 420 363 326 237 228
135 76 75 42 36 17
BspEI T'CCGG_A
Cuts at: 0 3175 4032
Size: 3175 857
BspHI T'CATG_A
Cuts at: 0 849 1974 2999 3146 4032
Size: 849 1125 1025 147 886
Fragments arranged by size:
1125 1025 886 849 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 422 1170 3359 4032
Size: 422 748 2189 673
Fragments arranged by size:
2189 748 673 422
BsrBI CCG'CTC
Cuts at: 0 28 1040 4032
Size: 28 1012 2992
Fragments arranged by size:
2992 1012 28
BsrFI r'CCGG_y
Cuts at: 0 3483 4032
Size: 3483 549
BssSI C'ACGA_G
Cuts at: 0 970 3472 3589 4032
Size: 970 2502 117 443
Fragments arranged by size:
2502 970 443 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2798 4032
Size: 2798 1234
BstEII G'GTnAC_C
Cuts at: 0 3703 4032
Size: 3703 329
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 468 1458 3726 4032
Size: 228 240 990 2268 306
Fragments arranged by size:
2268 990 306 240 228
BstYI r'GATC_y
Cuts at: 0 1081 1343 3082 3287 3858 4013 4032
Size: 1081 262 1739 205 571 155 19
Fragments arranged by size:
1739 1081 571 262 205 155 19
Bsu36I CC'TnA_GG
Cuts at: 0 3306 3641 4032
Size: 3306 335 391
Fragments arranged by size:
3306 391 335
BtgI C'CryG_G
Cuts at: 0 1406 1451 1499 2120 2675 3435 3578 4032
Size: 1406 45 48 621 555 760 143 454
Fragments arranged by size:
1406 760 621 555 454 143 48 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1050 1436 3351 4032
Size: 1050 386 1915 681
Fragments arranged by size:
1915 1050 681 386
BtsI GCAGTG_nn'
Cuts at: 0 1357 1836 4032
Size: 1357 479 2196
Fragments arranged by size:
2196 1357 479
ClaI AT'CG_AT
Cuts at: 0 3745 4032
Size: 3745 287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 893 928 4032
Size: 893 35 3104
Fragments arranged by size:
3104 893 35
DraI TTT'AAA
Cuts at: 0 866 1553 4032
Size: 866 687 2479
Fragments arranged by size:
2479 866 687
DraIII CAC_nnn'GTG
Cuts at: 0 3164 4032
Size: 3164 868
EaeI y'GGCC_r
Cuts at: 0 599 1379 2117 2214 2471 2588 2867 3269
Size: 599 780 738 97 257 117 279 402
Cuts at: 3269 3575 4032
Size: 306 457
Fragments arranged by size:
780 738 599 457 402 306 279 257
117 97
EagI C'GGCC_G
Cuts at: 0 2867 4032
Size: 2867 1165
EarI CTCTTCn'nnn_
Cuts at: 0 65 71 767 1024 1700 2483 3516 3977
Size: 65 6 696 257 676 783 1033 461
Cuts at: 3977 4032
Size: 55
Fragments arranged by size:
1033 783 696 676 461 257 65 55
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 132 3310 4032
Size: 132 3178 722
Fragments arranged by size:
3178 722 132
EcoICRI GAG'CTC
Cuts at: 0 1716 4032
Size: 1716 2316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 230 423 976 1027 1336 1630 1678
Size: 185 45 193 553 51 309 294 48
Cuts at: 1678 1681 2032 2950 3250 3789 3874 4032
Size: 3 351 918 300 539 85 158
Fragments arranged by size:
918 553 539 351 309 300 294 193
185 158 85 51 48 45 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 152 1047 1645 2488 4032
Size: 152 895 598 843 1544
Fragments arranged by size:
1544 895 843 598 152
EcoO109I rG'GnC_Cy
Cuts at: 0 135 812 3782 4032
Size: 135 677 2970 250
Fragments arranged by size:
2970 677 250 135
EcoRI G'AATT_C
Cuts at: 0 2354 4032
Size: 2354 1678
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2980 4032
Size: 2980 1052
FspI TGC'GCA
Cuts at: 0 823 4032
Size: 823 3209
HaeII r_GCGC'y
Cuts at: 0 335 3919 3994 4032
Size: 335 3584 75 38
Fragments arranged by size:
3584 335 75 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 606 638 1044 1066 1076 1098
Size: 98 32 476 32 406 22 10 22
Cuts at: 1098 1153 1185 2275 2307 3170 3202 3515 3547
Size: 55 32 1090 32 863 32 313 32
Cuts at: 3547 3754 3786 3808 3840 3850 3882 4032
Size: 207 32 22 32 10 32 150
Fragments arranged by size:
1090 863 476 406 313 207 150 98
55 32 32 32 32 32 32 32
32 32 22 22 22 10 10
HincII GTy'rAC
Cuts at: 0 267 1122 1215 2457 3045 4032
Size: 267 855 93 1242 588 987
Fragments arranged by size:
1242 987 855 588 267 93
HindIII A'AGCT_T
Cuts at: 0 1819 4032
Size: 1819 2213
KasI G'GCGC_C
Cuts at: 0 3990 4032
Size: 3990 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 562 2547 4032
Size: 434 128 1985 1485
Fragments arranged by size:
1985 1485 434 128
MscI TGG'CCA
Cuts at: 0 1381 2216 2473 2590 3577 4032
Size: 1381 835 257 117 987 455
Fragments arranged by size:
1381 987 835 455 257 117
MslI CAynn'nnrTG
Cuts at: 0 226 322 1498 2578 2998 3199 3271 4032
Size: 226 96 1176 1080 420 201 72 761
Fragments arranged by size:
1176 1080 761 420 226 201 96 72
MspA1I CmG'CkG
Cuts at: 0 584 1275 2229 2413 3228 3405 4032
Size: 584 691 954 184 815 177 627
Fragments arranged by size:
954 815 691 627 584 184 177
NarI GG'CG_CC
Cuts at: 0 3991 4032
Size: 3991 41
NcoI C'CATG_G
Cuts at: 0 1451 4032
Size: 1451 2581
NsiI A_TGCA'T
Cuts at: 0 1467 4032
Size: 1467 2565
NspI r_CATG'y
Cuts at: 0 1523 1656 2038 2577 2805 3390 4032
Size: 1523 133 382 539 228 585 642
Fragments arranged by size:
1523 642 585 539 382 228 133
PciI A'CATG_T
Cuts at: 0 1519 2034 4032
Size: 1519 515 1998
Fragments arranged by size:
1998 1519 515
PflMI CCAn_nnn'nTGG
Cuts at: 0 2718 4032
Size: 2718 1314
PfoI T'CCnGG_A
Cuts at: 0 299 1646 4032
Size: 299 1347 2386
Fragments arranged by size:
2386 1347 299
PpuMI rG'GwC_Cy
Cuts at: 0 812 3782 4032
Size: 812 2970 250
Fragments arranged by size:
2970 812 250
PsiI TTA'TAA
Cuts at: 0 2627 2933 4032
Size: 2627 306 1099
Fragments arranged by size:
2627 1099 306
PstI C_TGCA'G
Cuts at: 0 36 1352 2353 3356 4032
Size: 36 1316 1001 1003 676
Fragments arranged by size:
1316 1003 1001 676 36
PvuI CG_AT'CG
Cuts at: 0 1581 4032
Size: 1581 2451
PvuII CAG'CTG
Cuts at: 0 2229 2413 3228 4032
Size: 2229 184 815 804
Fragments arranged by size:
2229 815 804 184
SacI G_AGCT'C
Cuts at: 0 1718 4032
Size: 1718 2314
SalI G'TCGA_C
Cuts at: 0 1213 4032
Size: 1213 2819
SanDI GG'GwC_CC
Cuts at: 0 3782 4032
Size: 3782 250
SapI GCTCTTCn'nnn_
Cuts at: 0 767 3977 4032
Size: 767 3210 55
Fragments arranged by size:
3210 767 55
SbfI CC_TGCA'GG
Cuts at: 0 3356 4032
Size: 3356 676
SfcI C'TryA_G
Cuts at: 0 32 1348 1593 2349 3215 3352 4032
Size: 32 1316 245 756 866 137 680
Fragments arranged by size:
1316 866 756 680 245 137 32
SfoI GGC'GCC
Cuts at: 0 3992 4032
Size: 3992 40
SmaI CCC'GGG
Cuts at: 0 2373 4032
Size: 2373 1659
SmlI C'TyrA_G
Cuts at: 0 1090 1243 1510 3343 4032
Size: 1090 153 267 1833 689
Fragments arranged by size:
1833 1090 689 267 153
SnaBI TAC'GTA
Cuts at: 0 1233 4032
Size: 1233 2799
SphI G_CATG'C
Cuts at: 0 2805 4032
Size: 2805 1227
StyI C'CwwG_G
Cuts at: 0 652 731 995 1451 3902 4032
Size: 652 79 264 456 2451 130
Fragments arranged by size:
2451 652 456 264 130 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 201 547 2412 2725 4032
Size: 201 346 1865 313 1307
Fragments arranged by size:
1865 1307 346 313 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 738 770 1619 1651 2672 2704 3458 3490
Size: 738 32 849 32 1021 32 754 32
Cuts at: 3490 4032
Size: 542
Fragments arranged by size:
1021 849 754 738 542 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1056 4032
Size: 1056 2976
XbaI T'CTAG_A
Cuts at: 0 3813 4032
Size: 3813 219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1002 3083 3618 3915 4032
Size: 1002 2081 535 297 117
Fragments arranged by size:
2081 1002 535 297 117
XmaI C'CCGG_G
Cuts at: 0 2371 4032
Size: 2371 1661
XmnI GAAnn'nnTTC
Cuts at: 0 1108 1486 2922 4032
Size: 1108 378 1436 1110
Fragments arranged by size:
1436 1110 1108 378
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AleI AlwNI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BclI BfrBI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI NsiI NspI PciI PflMI PfoI PpuMI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI SfcI SfoI SmaI SmlI SnaBI SphI StyI
TaqII TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AlfI AloI ApaI
AscI AseI AsiSI AvrII BcgI BciVI BlpI BmtI
BsiWI BsmI BsrDI BsrGI BssHII BstBI BstZ17I DrdI
EcoRV FseI FspAI HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI RsrII SacII
ScaI SexAI SfiI SgfI SgrAI SpeI SrfI SspI
StuI SwaI TatI XhoI ZraI