Restriction map for
pF1KA0302
(Linear) (Six-base) MAPSORT of: KIAA0302.seq Check: 6828 from: 1 to: 7170
KIAA0302.orf
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5562 7170
Size: 5562 1608
AccI GT'mk_AC
Cuts at: 0 1148 6134 6595 7170
Size: 1148 4986 461 575
Fragments arranged by size:
4986 1148 575 461
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 2110 2776 3290 3740 3889 5056 5659
Size: 703 1407 666 514 450 149 1167 603
Cuts at: 5659 6010 7031 7131 7170
Size: 351 1021 100 39
Fragments arranged by size:
1407 1167 1021 703 666 603 514 450
351 149 100 39
AfeI AGC'GCT
Cuts at: 0 1483 5413 6114 7170
Size: 1483 3930 701 1056
Fragments arranged by size:
3930 1483 1056 701
AflIII A'CryG_T
Cuts at: 0 3908 6734 6879 7170
Size: 3908 2826 145 291
Fragments arranged by size:
3908 2826 291 145
AgeI A'CCGG_T
Cuts at: 0 2020 7170
Size: 2020 5150
AleI CACnn'nnGTG
Cuts at: 0 1223 2728 7170
Size: 1223 1505 4442
Fragments arranged by size:
4442 1505 1223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4573 5807 7170
Size: 4573 1234 1363
Fragments arranged by size:
4573 1363 1234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4390 4422 5111 5143 7170
Size: 4390 32 689 32 2027
Fragments arranged by size:
4390 2027 689 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1617 1674 2226 2754 3438 4091 4281 4596
Size: 1617 57 552 528 684 653 190 315
Cuts at: 4596 4896 5388 5706 6355 6453 6725 7170
Size: 300 492 318 649 98 272 445
Fragments arranged by size:
1617 684 653 649 552 528 492 445
318 315 300 272 190 98 57
ApaI G_GGCC'C
Cuts at: 0 1905 2087 2373 3351 5670 6533 6565 6843
Size: 1905 182 286 978 2319 863 32 278
Cuts at: 6843 7170
Size: 327
Fragments arranged by size:
2319 1905 978 863 327 286 278 182
32
ApaLI G'TGCA_C
Cuts at: 0 391 2898 7170
Size: 391 2507 4272
Fragments arranged by size:
4272 2507 391
ApoI r'AATT_y
Cuts at: 0 5203 7170
Size: 5203 1967
AvaI C'yCGr_G
Cuts at: 0 215 283 294 2317 2461 2516 3038 3056
Size: 215 68 11 2023 144 55 522 18
Cuts at: 3056 3395 4386 5219 5807 5873 6533 6557 6629
Size: 339 991 833 588 66 660 24 72
Cuts at: 6629 7037 7049 7170
Size: 408 12 121
Fragments arranged by size:
2023 991 833 660 588 522 408 339
215 144 121 72 68 66 55 24
18 12 11
AvrII C'CTAG_G
Cuts at: 0 2958 7170
Size: 2958 4212
BamHI G'GATC_C
Cuts at: 0 335 762 3680 5126 7170
Size: 335 427 2918 1446 2044
Fragments arranged by size:
2918 2044 1446 427 335
BanI G'GyrC_C
Cuts at: 0 554 1682 2023 2478 4445 5981 7023 7170
Size: 554 1128 341 455 1967 1536 1042 147
Fragments arranged by size:
1967 1536 1128 1042 554 455 341 147
BanII G_rGCy'C
Cuts at: 0 412 1250 1905 2087 2373 3351 3378 3692
Size: 412 838 655 182 286 978 27 314
Cuts at: 3692 4028 4625 4770 5282 5670 6443 6533 6565
Size: 336 597 145 512 388 773 90 32
Cuts at: 6565 6843 6952 7170
Size: 278 109 218
Fragments arranged by size:
978 838 773 655 597 512 412 388
336 314 286 278 218 182 145 109
90 32 27
BbeI G_GCGC'C
Cuts at: 0 4449 5985 7170
Size: 4449 1536 1185
Fragments arranged by size:
4449 1536 1185
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 571 776 2935 3811 6876 6983 7170
Size: 515 56 205 2159 876 3065 107 187
Fragments arranged by size:
3065 2159 876 515 205 187 107 56
BbvCI CC'TCA_GC
Cuts at: 0 274 1958 1995 2865 3551 5554 5624 7088
Size: 274 1684 37 870 686 2003 70 1464
Cuts at: 7088 7170
Size: 82
Fragments arranged by size:
2003 1684 1464 870 686 274 82 70
37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2197 2231 2809 2843 3961 3995 4441 4475
Size: 2197 34 578 34 1118 34 446 34
Cuts at: 4475 7170
Size: 2695
Fragments arranged by size:
2695 2197 1118 578 446 34 34 34
34
BfrBI ATG'CAT
Cuts at: 0 718 7170
Size: 718 6452
BglI GCCn_nnn'nGGC
Cuts at: 0 904 1747 2545 3310 3976 4795 5314 7170
Size: 904 843 798 765 666 819 519 1856
Fragments arranged by size:
1856 904 843 819 798 765 666 519
BglII A'GATC_T
Cuts at: 0 5948 7170
Size: 5948 1222
BlpI GC'TnA_GC
Cuts at: 0 1321 2572 3562 4154 4691 6116 7170
Size: 1321 1251 990 592 537 1425 1054
Fragments arranged by size:
1425 1321 1251 1054 990 592 537
Bme1580I G_kGCm'C
Cuts at: 0 395 1905 2028 2087 2220 2330 2373 2483
Size: 395 1510 123 59 133 110 43 110
Cuts at: 2483 2902 3351 4182 4869 5430 5670 6533 6565
Size: 419 449 831 687 561 240 863 32
Cuts at: 6565 6843 7028 7170
Size: 278 185 142
Fragments arranged by size:
1510 863 831 687 561 449 419 395
278 240 185 142 133 123 110 110
59 43 32
BmgBI CAC'GTC
Cuts at: 0 774 3909 7170
Size: 774 3135 3261
Fragments arranged by size:
3261 3135 774
BmrI ACTGGGnnnn_n'
Cuts at: 0 105 751 2175 3408 6404 7063 7170
Size: 105 646 1424 1233 2996 659 107
Fragments arranged by size:
2996 1424 1233 659 646 107 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 309 4401 5967 6966 7170
Size: 309 4092 1566 999 204
Fragments arranged by size:
4092 1566 999 309 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 633 1225 1288 1384 1612 1930 2122
Size: 367 266 592 63 96 228 318 192
Cuts at: 2122 2395 2509 2671 2680 2710 3043 3460 3595
Size: 273 114 162 9 30 333 417 135
Cuts at: 3595 3661 3715 4123 4204 4216 4771 4918 5069
Size: 66 54 408 81 12 555 147 151
Cuts at: 5069 5581 5633 6235 6308 7022 7170
Size: 512 52 602 73 714 148
Fragments arranged by size:
714 602 592 555 512 417 408 367
333 318 273 266 228 192 162 151
148 147 135 114 96 81 73 66
63 54 52 30 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 274 1009 1958 1995 2865 2872 3092 3551
Size: 274 735 949 37 870 7 220 459
Cuts at: 3551 5554 5624 6510 7088 7170
Size: 2003 70 886 578 82
Fragments arranged by size:
2003 949 886 870 735 578 459 274
220 82 70 37 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 2436 4054 4249 5380 6493 7170
Size: 361 2075 1618 195 1131 1113 677
Fragments arranged by size:
2075 1618 1131 1113 677 361 195
BsaI GGTCTCn'nnnn_
Cuts at: 0 140 1306 2006 2677 2899 4070 4099 7170
Size: 140 1166 700 671 222 1171 29 3071
Fragments arranged by size:
3071 1171 1166 700 671 222 140 29
BsaAI yAC'GTr
Cuts at: 0 224 7170
Size: 224 6946
BsaBI GATnn'nnATC
Cuts at: 0 4996 7170
Size: 4996 2174
BsaHI Gr'CG_yC
Cuts at: 0 1454 2411 2832 3165 3665 4446 5982 7170
Size: 1454 957 421 333 500 781 1536 1188
Fragments arranged by size:
1536 1454 1188 957 781 500 421 333
BsaWI w'CCGG_w
Cuts at: 0 2020 5472 7170
Size: 2020 3452 1698
Fragments arranged by size:
3452 2020 1698
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 511 541 2269 2299 2323 2353
Size: 394 30 87 30 1728 30 24 30
Cuts at: 2353 4390 4420 5872 5902 7170
Size: 2037 30 1452 30 1268
Fragments arranged by size:
2037 1728 1452 1268 394 87 30 30
30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 1570 1573 2224 2392 2395 2518 2620
Size: 271 1299 3 651 168 3 123 102
Cuts at: 2620 3400 3478 3786 4414 5641 5880 5959 6211
Size: 780 78 308 628 1227 239 79 252
Cuts at: 6211 6250 6283 6286 6289 7170
Size: 39 33 3 3 881
Fragments arranged by size:
1299 1227 881 780 651 628 308 271
252 239 168 123 102 79 78 39
33 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 74 2079 2219 2322 2974 3137 3180 3458
Size: 74 2005 140 103 652 163 43 278
Cuts at: 3458 3468 4091 4211 4561 4769 5163 5287 5669
Size: 10 623 120 350 208 394 124 382
Cuts at: 5669 6120 6377 6456 6891 7027 7170
Size: 451 257 79 435 136 143
Fragments arranged by size:
2005 652 623 451 435 394 382 350
278 257 208 163 143 140 136 124
120 103 79 74 43 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1462 1699 1765 2183 2488 2689 3148
Size: 193 1269 237 66 418 305 201 459
Cuts at: 3148 4066 4201 4385 4685 5285 5404 5470 5596
Size: 918 135 184 300 600 119 66 126
Cuts at: 5596 6411 7170
Size: 815 759
Fragments arranged by size:
1269 918 815 759 600 459 418 305
300 237 201 193 184 135 126 119
66 66
BsiEI CG_ry'CG
Cuts at: 0 974 1293 1437 2076 5637 5942 6134 7170
Size: 974 319 144 639 3561 305 192 1036
Fragments arranged by size:
3561 1036 974 639 319 305 192 144
BsiHKAI G_wGCw'C
Cuts at: 0 293 395 1220 1250 2902 3563 3658 4028
Size: 293 102 825 30 1652 661 95 370
Cuts at: 4028 4659 4976 4987 5078 6479 6952 7106 7170
Size: 631 317 11 91 1401 473 154 64
Fragments arranged by size:
1652 1401 825 661 631 473 370 317
293 154 102 95 91 64 30 11
BsiWI C'GTAC_G
Cuts at: 0 5399 7170
Size: 5399 1771
BsmI GAATG_Cn'
Cuts at: 0 718 720 3961 6975 7170
Size: 718 2 3241 3014 195
Fragments arranged by size:
3241 3014 718 195 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1405 7170
Size: 1405 5765
Bsp1286I G_dGCh'C
Cuts at: 0 293 395 412 1220 1250 1905 2028 2087
Size: 293 102 17 808 30 655 123 59
Cuts at: 2087 2220 2330 2373 2483 2902 3351 3378 3563
Size: 133 110 43 110 419 449 27 185
Cuts at: 3563 3658 3692 4028 4182 4625 4659 4770 4869
Size: 95 34 336 154 443 34 111 99
Cuts at: 4869 4976 4987 5078 5282 5430 5670 6443 6479
Size: 107 11 91 204 148 240 773 36
Cuts at: 6479 6533 6565 6843 6952 7028 7106 7170
Size: 54 32 278 109 76 78 64
Fragments arranged by size:
808 773 655 449 443 419 336 293
278 240 204 185 154 148 133 123
111 110 110 109 107 102 99 95
91 78 76 64 59 54 43 36
34 34 32 30 27 17 11
BspEI T'CCGG_A
Cuts at: 0 5472 7170
Size: 5472 1698
BspHI T'CATG_A
Cuts at: 0 3650 4577 7170
Size: 3650 927 2593
Fragments arranged by size:
3650 2593 927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 278 562 671 1625 1712 3941 4088 4565
Size: 278 284 109 954 87 2229 147 477
Cuts at: 4565 4666 5363 5562 5789 6581 7170
Size: 101 697 199 227 792 589
Fragments arranged by size:
2229 954 792 697 589 477 284 278
227 199 147 109 101 87
BsrBI CCG'CTC
Cuts at: 0 1200 1510 1575 1842 2072 2190 2493 3015
Size: 1200 310 65 267 230 118 303 522
Cuts at: 3015 3091 3345 4518 4647 5055 6240 6279 7170
Size: 76 254 1173 129 408 1185 39 891
Fragments arranged by size:
1200 1185 1173 891 522 408 310 303
267 254 230 129 118 76 65 39
BsrDI GCAATG_nn'
Cuts at: 0 2738 5282 6584 6977 7170
Size: 2738 2544 1302 393 193
Fragments arranged by size:
2738 2544 1302 393 193
BsrFI r'CCGG_y
Cuts at: 0 653 989 1166 1655 2020 2075 2396 3004
Size: 653 336 177 489 365 55 321 608
Cuts at: 3004 3079 3133 3279 3365 3488 4442 5029 5591
Size: 75 54 146 86 123 954 587 562
Cuts at: 5591 5696 7017 7170
Size: 105 1321 153
Fragments arranged by size:
1321 954 653 608 587 562 489 365
336 321 177 153 146 123 105 86
75 55 54
BsrGI T'GTAC_A
Cuts at: 0 242 591 7170
Size: 242 349 6579
Fragments arranged by size:
6579 349 242
BssSI C'ACGA_G
Cuts at: 0 1219 1339 1717 3862 4723 4969 5158 6163
Size: 1219 120 378 2145 861 246 189 1005
Cuts at: 6163 6951 7170
Size: 788 219
Fragments arranged by size:
2145 1219 1005 861 788 378 246 219
189 120
BstAPI GCAn_nnn'nTGC
Cuts at: 0 390 3849 4278 4893 5706 6979 7170
Size: 390 3459 429 615 813 1273 191
Fragments arranged by size:
3459 1273 813 615 429 390 191
BstBI TT'CG_AA
Cuts at: 0 2388 7170
Size: 2388 4782
BstEII G'GTnAC_C
Cuts at: 0 2131 3390 7170
Size: 2131 1259 3780
Fragments arranged by size:
3780 2131 1259
BstXI CCAn_nnnn'nTGG
Cuts at: 0 759 4344 4539 5268 7170
Size: 759 3585 195 729 1902
Fragments arranged by size:
3585 1902 759 729 195
BstYI r'GATC_y
Cuts at: 0 335 482 762 3680 5126 5826 5948 7170
Size: 335 147 280 2918 1446 700 122 1222
Fragments arranged by size:
2918 1446 1222 700 335 280 147 122
Bsu36I CC'TnA_GG
Cuts at: 0 3148 3293 4639 7170
Size: 3148 145 1346 2531
Fragments arranged by size:
3148 2531 1346 145
BtgI C'CryG_G
Cuts at: 0 860 899 1055 1457 2726 3513 3659 3684
Size: 860 39 156 402 1269 787 146 25
Cuts at: 3684 4394 4526 4538 5312 5393 5651 5822 6382
Size: 710 132 12 774 81 258 171 560
Cuts at: 6382 6810 7170
Size: 428 360
Fragments arranged by size:
1269 860 787 774 710 560 428 402
360 258 171 156 146 132 81 39
25 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1488 1722 3015 4499 4799 7170
Size: 1488 234 1293 1484 300 2371
Fragments arranged by size:
2371 1488 1484 1293 300 234
BtsI GCAGTG_nn'
Cuts at: 0 339 364 961 1454 2296 2623 3104 6191
Size: 339 25 597 493 842 327 481 3087
Cuts at: 6191 7170
Size: 979
Fragments arranged by size:
3087 979 842 597 493 481 339 327
25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 9 44 2164 2199 7170
Size: 9 35 2120 35 4971
Fragments arranged by size:
4971 2120 35 35 9
DraIII CAC_nnn'GTG
Cuts at: 0 194 1524 4893 6412 7170
Size: 194 1330 3369 1519 758
Fragments arranged by size:
3369 1519 1330 758 194
DrdI GACnn_nn'nnGTC
Cuts at: 0 2662 2785 7170
Size: 2662 123 4385
Fragments arranged by size:
4385 2662 123
EaeI y'GGCC_r
Cuts at: 0 655 857 998 1277 1434 1472 1559 1847
Size: 655 202 141 279 157 38 87 288
Cuts at: 1847 2073 2123 2204 2398 3002 3081 3101 3281
Size: 226 50 81 194 604 79 20 180
Cuts at: 3281 3702 3968 4529 4910 4943 5270 5315 5390
Size: 421 266 561 381 33 327 45 75
Cuts at: 5390 5484 5586 5634 5654 5921 5939 6342 6385
Size: 94 102 48 20 267 18 403 43
Cuts at: 6385 6833 7170
Size: 448 337
Fragments arranged by size:
655 604 561 448 421 403 381 337
327 288 279 267 266 226 202 194
180 157 141 102 94 87 81 79
75 50 48 45 43 38 33 20
20 18
EagI C'GGCC_G
Cuts at: 0 1434 2073 5634 5939 7170
Size: 1434 639 3561 305 1231
Fragments arranged by size:
3561 1434 1231 639 305
EarI CTCTTCn'nnn_
Cuts at: 0 1111 1637 3167 3427 6051 7007 7170
Size: 1111 526 1530 260 2624 956 163
Fragments arranged by size:
2624 1530 1111 956 526 260 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1228 1243 2020 4813 4990 5086 6297 7170
Size: 1228 15 777 2793 177 96 1211 873
Fragments arranged by size:
2793 1228 1211 873 777 177 96 15
EcoICRI GAG'CTC
Cuts at: 0 1248 4026 6950 7170
Size: 1248 2778 2924 220
Fragments arranged by size:
2924 2778 1248 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 633 703 1225 1288 1384 1612 1930
Size: 367 266 70 522 63 96 228 318
Cuts at: 1930 2110 2122 2395 2509 2671 2680 2710 2776
Size: 180 12 273 114 162 9 30 66
Cuts at: 2776 3043 3290 3460 3595 3661 3715 3740 3889
Size: 267 247 170 135 66 54 25 149
Cuts at: 3889 4123 4204 4216 4771 4918 5056 5069 5581
Size: 234 81 12 555 147 138 13 512
Cuts at: 5581 5633 5659 6010 6235 6308 7022 7031 7131
Size: 52 26 351 225 73 714 9 100
Cuts at: 7131 7170
Size: 39
Fragments arranged by size:
714 555 522 512 367 351 318 273
267 266 247 234 228 225 180 170
162 149 147 138 135 114 100 96
81 73 70 66 66 63 54 52
39 30 26 25 13 12 12 9
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1942 2104 2206 2377 3292 3382 3505 6509
Size: 1942 162 102 171 915 90 123 3004
Cuts at: 6509 7170
Size: 661
Fragments arranged by size:
3004 1942 915 661 171 162 123 102
90
EcoO109I rG'GnC_Cy
Cuts at: 0 78 237 852 1614 1671 1701 2083 2084
Size: 78 159 615 762 57 30 382 1
Cuts at: 2084 2199 2370 2585 2655 2955 3018 3144 3303
Size: 115 171 215 70 300 63 126 159
Cuts at: 3303 3444 4239 4412 4554 5508 5550 6330 6502
Size: 141 795 173 142 954 42 780 172
Cuts at: 6502 6530 6561 6722 6839 7093 7170
Size: 28 31 161 117 254 77
Fragments arranged by size:
954 795 780 762 615 382 300 254
215 173 172 171 161 159 159 142
141 126 117 115 78 77 70 63
57 42 31 30 28 1
EcoRV GAT'ATC
Cuts at: 0 4994 7170
Size: 4994 2176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 201 5748 6912 7170
Size: 201 5547 1164 258
Fragments arranged by size:
5547 1164 258 201
FseI GG_CCGG'CC
Cuts at: 0 3283 7170
Size: 3283 3887
FspI TGC'GCA
Cuts at: 0 1240 3214 6109 7170
Size: 1240 1974 2895 1061
Fragments arranged by size:
2895 1974 1240 1061
HaeII r_GCGC'y
Cuts at: 0 915 1338 1485 1775 2560 4092 4449 5067
Size: 915 423 147 290 785 1532 357 618
Cuts at: 5067 5261 5415 5520 5985 6116 7170
Size: 194 154 105 465 131 1054
Fragments arranged by size:
1532 1054 915 785 618 465 423 357
290 194 154 147 131 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 467 499 511 543 661 693
Size: 97 32 338 32 12 32 118 32
Cuts at: 693 784 816 2323 2355 5803 5835 5872 5904
Size: 91 32 1507 32 3448 32 37 32
Cuts at: 5904 5947 5979 6540 6572 7170
Size: 43 32 561 32 598
Fragments arranged by size:
3448 1507 598 561 338 118 97 91
43 37 32 32 32 32 32 32
32 32 32 32 12
HincII GTy'rAC
Cuts at: 0 2292 2790 4254 5778 6135 7170
Size: 2292 498 1464 1524 357 1035
Fragments arranged by size:
2292 1524 1464 1035 498 357
HindIII A'AGCT_T
Cuts at: 0 1123 6220 7170
Size: 1123 5097 950
Fragments arranged by size:
5097 1123 950
KasI G'GCGC_C
Cuts at: 0 4445 5981 7170
Size: 4445 1536 1189
Fragments arranged by size:
4445 1536 1189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1817 4043 7170
Size: 1817 2226 3127
Fragments arranged by size:
3127 2226 1817
MscI TGG'CCA
Cuts at: 0 1000 1849 3103 4531 5272 5923 6835 7170
Size: 1000 849 1254 1428 741 651 912 335
Fragments arranged by size:
1428 1254 1000 912 849 741 651 335
MslI CAynn'nnrTG
Cuts at: 0 496 1223 1741 2728 3595 4223 4484 5902
Size: 496 727 518 987 867 628 261 1418
Cuts at: 5902 7060 7170
Size: 1158 110
Fragments arranged by size:
1418 1158 987 867 727 628 518 496
261 110
MspA1I CmG'CkG
Cuts at: 0 74 611 1029 1275 1375 1433 1713 1830
Size: 74 537 418 246 100 58 280 117
Cuts at: 1830 1891 2167 2223 2728 3711 4215 4938 5395
Size: 61 276 56 505 983 504 723 457
Cuts at: 5395 5703 5824 5970 5995 6030 7170
Size: 308 121 146 25 35 1140
Fragments arranged by size:
1140 983 723 537 505 504 457 418
308 280 276 246 146 121 117 100
74 61 58 56 35 25
NaeI GCC'GGC
Cuts at: 0 1168 1657 2077 3006 3081 3281 3367 3490
Size: 1168 489 420 929 75 200 86 123
Cuts at: 3490 4444 5031 5698 7170
Size: 954 587 667 1472
Fragments arranged by size:
1472 1168 954 929 667 587 489 420
200 123 86 75
NarI GG'CG_CC
Cuts at: 0 4446 5982 7170
Size: 4446 1536 1188
Fragments arranged by size:
4446 1536 1188
NcoI C'CATG_G
Cuts at: 0 899 3659 4526 4538 7170
Size: 899 2760 867 12 2632
Fragments arranged by size:
2760 2632 899 867 12
NgoMIV G'CCGG_C
Cuts at: 0 1166 1655 2075 3004 3079 3279 3365 3488
Size: 1166 489 420 929 75 200 86 123
Cuts at: 3488 4442 5029 5696 7170
Size: 954 587 667 1474
Fragments arranged by size:
1474 1166 954 929 667 587 489 420
200 123 86 75
NruI TCG'CGA
Cuts at: 0 4463 7170
Size: 4463 2707
NsiI A_TGCA'T
Cuts at: 0 720 7170
Size: 720 6450
NspI r_CATG'y
Cuts at: 0 333 3322 5185 5646 6883 7170
Size: 333 2989 1863 461 1237 287
Fragments arranged by size:
2989 1863 1237 461 333 287
PasI CC'CwG_GG
Cuts at: 0 1798 2137 2952 3378 3447 6843 7170
Size: 1798 339 815 426 69 3396 327
Fragments arranged by size:
3396 1798 815 426 339 327 69
PciI A'CATG_T
Cuts at: 0 6879 7170
Size: 6879 291
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 611 900 2118 2226 4728 4896 4938
Size: 345 266 289 1218 108 2502 168 42
Cuts at: 4938 5163 5439 7170
Size: 225 276 1731
Fragments arranged by size:
2502 1731 1218 345 289 276 266 225
168 108 42
PfoI T'CCnGG_A
Cuts at: 0 254 1610 3692 5129 5747 7170
Size: 254 1356 2082 1437 618 1423
Fragments arranged by size:
2082 1437 1423 1356 618 254
PmlI CAC'GTG
Cuts at: 0 224 7170
Size: 224 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4390 4422 5744 5776 7170
Size: 4390 32 1322 32 1394
Fragments arranged by size:
4390 1394 1322 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 78 237 1614 1671 1701 2655 3018 3144
Size: 78 159 1377 57 30 954 363 126
Cuts at: 3144 4239 4554 6330 6502 6722 7170
Size: 1095 315 1776 172 220 448
Fragments arranged by size:
1776 1377 1095 954 448 363 315 220
172 159 126 78 57 30
PshAI GACnn'nnGTC
Cuts at: 0 2935 7170
Size: 2935 4235
PspOMI G'GGCC_C
Cuts at: 0 1901 2083 2369 3347 5666 6529 6561 6839
Size: 1901 182 286 978 2319 863 32 278
Cuts at: 6839 7170
Size: 331
Fragments arranged by size:
2319 1901 978 863 331 286 278 182
32
PspXI vC'TCGA_Gb
Cuts at: 0 283 4386 6629 7170
Size: 283 4103 2243 541
Fragments arranged by size:
4103 2243 541 283
PstI C_TGCA'G
Cuts at: 0 371 573 713 956 1034 1467 1601 1664
Size: 371 202 140 243 78 433 134 63
Cuts at: 1664 1712 2513 2993 3203 4208 4454 4601 4700
Size: 48 801 480 210 1005 246 147 99
Cuts at: 4700 5072 5411 5516 5786 5975 6091 7170
Size: 372 339 105 270 189 116 1079
Fragments arranged by size:
1079 1005 801 480 433 372 371 339
270 246 243 210 202 189 147 140
134 116 105 99 78 63 48
PvuI CG_AT'CG
Cuts at: 0 974 7170
Size: 974 6196
PvuII CAG'CTG
Cuts at: 0 611 1029 1275 1375 1713 1830 2167 3711
Size: 611 418 246 100 338 117 337 1544
Cuts at: 3711 4215 4938 5703 5970 5995 6030 7170
Size: 504 723 765 267 25 35 1140
Fragments arranged by size:
1544 1140 765 723 611 504 418 338
337 267 246 117 100 35 25
SacI G_AGCT'C
Cuts at: 0 1250 4028 6952 7170
Size: 1250 2778 2924 218
Fragments arranged by size:
2924 2778 1250 218
SacII CC_GC'GG
Cuts at: 0 2729 5396 5825 7170
Size: 2729 2667 429 1345
Fragments arranged by size:
2729 2667 1345 429
SalI G'TCGA_C
Cuts at: 0 6133 7170
Size: 6133 1037
SapI GCTCTTCn'nnn_
Cuts at: 0 1111 7007 7170
Size: 1111 5896 163
Fragments arranged by size:
5896 1111 163
SbfI CC_TGCA'GG
Cuts at: 0 4208 4454 5072 7170
Size: 4208 246 618 2098
Fragments arranged by size:
4208 2098 618 246
SexAI A'CCwGG_T
Cuts at: 0 4889 6332 7170
Size: 4889 1443 838
Fragments arranged by size:
4889 1443 838
SfcI C'TryA_G
Cuts at: 0 367 569 709 952 1030 1428 1463 1597
Size: 367 202 140 243 78 398 35 134
Cuts at: 1597 1660 1708 2509 2989 3199 4204 4450 4597
Size: 63 48 801 480 210 1005 246 147
Cuts at: 4597 4696 5068 5407 5512 5782 5971 6087 7170
Size: 99 372 339 105 270 189 116 1083
Fragments arranged by size:
1083 1005 801 480 398 372 367 339
270 246 243 210 202 189 147 140
134 116 105 99 78 63 48 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3310 5314 7170
Size: 3310 2004 1856
Fragments arranged by size:
3310 2004 1856
SfoI GGC'GCC
Cuts at: 0 4447 5983 7170
Size: 4447 1536 1187
Fragments arranged by size:
4447 1536 1187
SmaI CCC'GGG
Cuts at: 0 217 2518 3040 3397 5221 5809 5875 6535
Size: 217 2301 522 357 1824 588 66 660
Cuts at: 6535 7039 7051 7170
Size: 504 12 119
Fragments arranged by size:
2301 1824 660 588 522 504 357 217
119 66 12
SmlI C'TyrA_G
Cuts at: 0 283 376 2415 3056 4033 4264 4386 5359
Size: 283 93 2039 641 977 231 122 973
Cuts at: 5359 6472 6629 7170
Size: 1113 157 541
Fragments arranged by size:
2039 1113 977 973 641 541 283 231
157 122 93
SphI G_CATG'C
Cuts at: 0 333 7170
Size: 333 6837
SrfI GCCC'GGGC
Cuts at: 0 2518 5875 7170
Size: 2518 3357 1295
Fragments arranged by size:
3357 2518 1295
SspI AAT'ATT
Cuts at: 0 6916 7170
Size: 6916 254
StuI AGG'CCT
Cuts at: 0 1963 2152 2281 2914 3235 4804 5344 5605
Size: 1963 189 129 633 321 1569 540 261
Cuts at: 5605 5662 6079 6169 6697 7170
Size: 57 417 90 528 473
Fragments arranged by size:
1963 1569 633 540 528 473 417 321
261 189 129 90 57
StyI C'CwwG_G
Cuts at: 0 447 533 899 2540 2958 3659 4121 4526
Size: 447 86 366 1641 418 701 462 405
Cuts at: 4526 4538 4850 6782 6929 7170
Size: 12 312 1932 147 241
Fragments arranged by size:
1932 1641 701 462 447 418 405 366
312 241 147 86 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 1939 1956 2589 2809 2965 4129 4497
Size: 33 1906 17 633 220 156 1164 368
Cuts at: 4497 4991 5227 6423 7170
Size: 494 236 1196 747
Fragments arranged by size:
1906 1196 1164 747 633 494 368 236
220 156 33 17
TatI w'GTAC_w
Cuts at: 0 53 242 591 6737 7170
Size: 53 189 349 6146 433
Fragments arranged by size:
6146 433 349 189 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 356 388 2585 2617 7170
Size: 356 32 2197 32 4553
Fragments arranged by size:
4553 2197 356 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1354 2319 3636 4935 7170
Size: 1354 965 1317 1299 2235
Fragments arranged by size:
2235 1354 1317 1299 965
XhoI C'TCGA_G
Cuts at: 0 283 3056 4386 6629 7170
Size: 283 2773 1330 2243 541
Fragments arranged by size:
2773 2243 1330 541 283
XmaI C'CCGG_G
Cuts at: 0 215 2516 3038 3395 5219 5807 5873 6533
Size: 215 2301 522 357 1824 588 66 660
Cuts at: 6533 7037 7049 7170
Size: 504 12 121
Fragments arranged by size:
2301 1824 660 588 522 504 357 215
121 66 12
XmnI GAAnn'nnTTC
Cuts at: 0 184 718 1606 3578 7170
Size: 184 534 888 1972 3592
Fragments arranged by size:
3592 1972 888 534 184
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI
SphI SrfI SspI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AscI AseI AsiSI
BaeI BciVI BclI BmtI BssHII BstZ17I ClaI DraI
EcoRI FspAI HpaI KpnI MfeI MluI NdeI NheI
NotI PacI PmeI PsiI PsrI RsrII SanDI ScaI
SgfI SgrAI SnaBI SpeI SwaI Tth111I XbaI ZraI