Restriction map for
pF1KA0308
(Linear) (Six-base) MAPSORT of: KIAA0308.seq Check: 5278 from: 1 to: 6036
KIAA0308.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 0 1250 6036
Size: 1250 4786
Acc65I G'GTAC_C
Cuts at: 0 2325 6036
Size: 2325 3711
AclI AA'CG_TT
Cuts at: 0 143 6036
Size: 143 5893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 535 1183 1766 2042 3539 3842 4172
Size: 189 346 648 583 276 1497 303 330
Cuts at: 4172 5344 6036
Size: 1172 692
Fragments arranged by size:
1497 1172 692 648 583 346 330 303
276 189
AflII C'TTAA_G
Cuts at: 0 477 4882 6036
Size: 477 4405 1154
Fragments arranged by size:
4405 1154 477
AflIII A'CryG_T
Cuts at: 0 1156 2689 5022 6036
Size: 1156 1533 2333 1014
Fragments arranged by size:
2333 1533 1156 1014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4451 5423 6036
Size: 4451 972 613
Fragments arranged by size:
4451 972 613
AlwNI CAG_nnn'CTG
Cuts at: 0 2071 2497 3754 4072 5281 5284 5397 6036
Size: 2071 426 1257 318 1209 3 113 639
Fragments arranged by size:
2071 1257 1209 639 426 318 113 3
ApoI r'AATT_y
Cuts at: 0 118 511 820 1459 2741 2944 4781 5112
Size: 118 393 309 639 1282 203 1837 331
Cuts at: 5112 6036
Size: 924
Fragments arranged by size:
1837 1282 924 639 393 331 309 203
118
AseI AT'TA_AT
Cuts at: 0 4814 6036
Size: 4814 1222
AvaI C'yCGr_G
Cuts at: 0 1115 2161 4893 6036
Size: 1115 1046 2732 1143
Fragments arranged by size:
2732 1143 1115 1046
AvrII C'CTAG_G
Cuts at: 0 3436 6036
Size: 3436 2600
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 713 746 6036
Size: 713 33 5290
Fragments arranged by size:
5290 713 33
BamHI G'GATC_C
Cuts at: 0 3063 6036
Size: 3063 2973
BanI G'GyrC_C
Cuts at: 0 418 612 2325 2769 4303 4591 6036
Size: 418 194 1713 444 1534 288 1445
Fragments arranged by size:
1713 1534 1445 444 418 288 194
BanII G_rGCy'C
Cuts at: 0 761 1944 4283 6036
Size: 761 1183 2339 1753
Fragments arranged by size:
2339 1753 1183 761
BbsI GAAGACnn'nnnn_
Cuts at: 0 448 3031 3506 3527 4469 4956 5570 5909
Size: 448 2583 475 21 942 487 614 339
Cuts at: 5909 6036
Size: 127
Fragments arranged by size:
2583 942 614 487 475 448 339 127
21
BbvCI CC'TCA_GC
Cuts at: 0 800 6036
Size: 800 5236
BclI T'GATC_A
Cuts at: 0 4136 4326 6036
Size: 4136 190 1710
Fragments arranged by size:
4136 1710 190
BglII A'GATC_T
Cuts at: 0 1401 2610 6036
Size: 1401 1209 3426
Fragments arranged by size:
3426 1401 1209
BmgBI CAC'GTC
Cuts at: 0 1531 6036
Size: 1531 4505
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 1321 4277 5799 6036
Size: 117 1204 2956 1522 237
Fragments arranged by size:
2956 1522 1204 237 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 3629 4277 5033 5465 5756 6036
Size: 1003 2626 648 756 432 291 280
Fragments arranged by size:
2626 1003 756 648 432 291 280
Bpu10I CC'TnA_GC
Cuts at: 0 760 800 6036
Size: 760 40 5236
Fragments arranged by size:
5236 760 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4760 6036
Size: 4760 1276
BsaI GGTCTCn'nnnn_
Cuts at: 0 1779 3605 4519 6036
Size: 1779 1826 914 1517
Fragments arranged by size:
1826 1779 1517 914
BsaAI yAC'GTr
Cuts at: 0 128 6036
Size: 128 5908
BsaBI GATnn'nnATC
Cuts at: 0 857 1181 1478 3726 6036
Size: 857 324 297 2248 2310
Fragments arranged by size:
2310 2248 857 324 297
BsaHI Gr'CG_yC
Cuts at: 0 2700 6036
Size: 2700 3336
BsaWI w'CCGG_w
Cuts at: 0 1574 1781 6036
Size: 1574 207 4255
Fragments arranged by size:
4255 1574 207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1776 1806 2600 2630 3018 3048 3425 3455
Size: 1776 30 794 30 388 30 377 30
Cuts at: 3455 3496 3526 5135 5165 6036
Size: 41 30 1609 30 871
Fragments arranged by size:
1776 1609 871 794 388 377 41 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 817 1988 3410 3484 3517 3885 3888
Size: 248 569 1171 1422 74 33 368 3
Cuts at: 3888 3891 3894 4716 5462 6036
Size: 3 3 822 746 574
Fragments arranged by size:
1422 1171 822 746 574 569 368 248
74 33 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2445 2562 3687 5410 6036
Size: 2445 117 1125 1723 626
Fragments arranged by size:
2445 1723 1125 626 117
BsiHKAI G_wGCw'C
Cuts at: 0 761 947 1944 4018 4473 4892 6036
Size: 761 186 997 2074 455 419 1144
Fragments arranged by size:
2074 1144 997 761 455 419 186
BsiWI C'GTAC_G
Cuts at: 0 124 6036
Size: 124 5912
BsmI GAATG_Cn'
Cuts at: 0 1828 2243 3007 4937 5429 6036
Size: 1828 415 764 1930 492 607
Fragments arranged by size:
1930 1828 764 607 492 415
BsmBI CGTCTCn'nnnn_
Cuts at: 0 573 2707 5232 6036
Size: 573 2134 2525 804
Fragments arranged by size:
2525 2134 804 573
Bsp1286I G_dGCh'C
Cuts at: 0 761 947 1944 4018 4283 4473 4892 6036
Size: 761 186 997 2074 265 190 419 1144
Fragments arranged by size:
2074 1144 997 761 419 265 190 186
BspEI T'CCGG_A
Cuts at: 0 1781 6036
Size: 1781 4255
BspHI T'CATG_A
Cuts at: 0 386 3237 4941 6036
Size: 386 2851 1704 1095
Fragments arranged by size:
2851 1704 1095 386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1853 2565 6036
Size: 1853 712 3471
Fragments arranged by size:
3471 1853 712
BsrDI GCAATG_nn'
Cuts at: 0 875 6036
Size: 875 5161
BsrFI r'CCGG_y
Cuts at: 0 4306 5431 6036
Size: 4306 1125 605
Fragments arranged by size:
4306 1125 605
BsrGI T'GTAC_A
Cuts at: 0 5823 6036
Size: 5823 213
BssSI C'ACGA_G
Cuts at: 0 2036 6036
Size: 2036 4000
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2497 3412 5281 5284 5397 6036
Size: 2497 915 1869 3 113 639
Fragments arranged by size:
2497 1869 915 639 113 3
BstBI TT'CG_AA
Cuts at: 0 1410 2148 6036
Size: 1410 738 3888
Fragments arranged by size:
3888 1410 738
BstXI CCAn_nnnn'nTGG
Cuts at: 0 883 1225 3221 3622 6036
Size: 883 342 1996 401 2414
Fragments arranged by size:
2414 1996 883 401 342
BstYI r'GATC_y
Cuts at: 0 519 1401 2004 2541 2610 3063 5346 5983
Size: 519 882 603 537 69 453 2283 637
Cuts at: 5983 6036
Size: 53
Fragments arranged by size:
2283 882 637 603 537 519 453 69
53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 330 6036
Size: 330 5706
BtsI GCAGTG_nn'
Cuts at: 0 2708 5395 6036
Size: 2708 2687 641
Fragments arranged by size:
2708 2687 641
ClaI AT'CG_AT
Cuts at: 0 1478 6036
Size: 1478 4558
DraI TTT'AAA
Cuts at: 0 2406 2574 6036
Size: 2406 168 3462
Fragments arranged by size:
3462 2406 168
DraIII CAC_nnn'GTG
Cuts at: 0 405 3276 6036
Size: 405 2871 2760
Fragments arranged by size:
2871 2760 405
DrdI GACnn_nn'nnGTC
Cuts at: 0 4068 6036
Size: 4068 1968
EaeI y'GGCC_r
Cuts at: 0 1890 4117 6036
Size: 1890 2227 1919
Fragments arranged by size:
2227 1919 1890
EarI CTCTTCn'nnn_
Cuts at: 0 1344 1407 1463 2856 3807 3882 3894 3912
Size: 1344 63 56 1393 951 75 12 18
Cuts at: 3912 5363 6036
Size: 1451 673
Fragments arranged by size:
1451 1393 1344 951 673 75 63 56
18 12
EcoICRI GAG'CTC
Cuts at: 0 759 1942 6036
Size: 759 1183 4094
Fragments arranged by size:
4094 1183 759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 535 1003 1183 1766 2042 3539 3629
Size: 189 346 468 180 583 276 1497 90
Cuts at: 3629 3842 4172 4277 5033 5344 5465 5756 6036
Size: 213 330 105 756 311 121 291 280
Fragments arranged by size:
1497 756 583 468 346 330 311 291
280 276 213 189 180 121 105 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2921 3499 5444 6036
Size: 2921 578 1945 592
Fragments arranged by size:
2921 1945 592 578
EcoO109I rG'GnC_Cy
Cuts at: 0 67 375 3153 6036
Size: 67 308 2778 2883
Fragments arranged by size:
2883 2778 308 67
EcoRI G'AATT_C
Cuts at: 0 4781 6036
Size: 4781 1255
EcoRV GAT'ATC
Cuts at: 0 1476 6036
Size: 1476 4560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 86 503 661 1423 1436 2827 3424 5681
Size: 86 417 158 762 13 1391 597 2257
Cuts at: 5681 6036
Size: 355
Fragments arranged by size:
2257 1391 762 597 417 355 158 86
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 1438 1470 1867 1869 1899 1901
Size: 69 32 1337 32 397 2 30 2
Cuts at: 1901 2584 2616 3018 3050 3102 3134 3496 3528
Size: 683 32 402 32 52 32 362 32
Cuts at: 3528 3604 3636 3730 3748 3762 3780 5833 5865
Size: 76 32 94 18 14 18 2053 32
Cuts at: 5865 5962 5980 5994 6012 6036
Size: 97 18 14 18 24
Fragments arranged by size:
2053 1337 683 402 397 362 97 94
76 69 52 32 32 32 32 32
32 32 32 30 24 18 18 18
18 14 14 2 2
HincII GTy'rAC
Cuts at: 0 1538 2638 4334 4415 4668 6036
Size: 1538 1100 1696 81 253 1368
Fragments arranged by size:
1696 1538 1368 1100 253 81
HindIII A'AGCT_T
Cuts at: 0 3187 6036
Size: 3187 2849
KpnI G_GTAC'C
Cuts at: 0 2329 6036
Size: 2329 3707
MfeI C'AATT_G
Cuts at: 0 305 1514 6036
Size: 305 1209 4522
Fragments arranged by size:
4522 1209 305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2041 3004 4127 5040 5818 6036
Size: 2041 963 1123 913 778 218
Fragments arranged by size:
2041 1123 963 913 778 218
MscI TGG'CCA
Cuts at: 0 4119 6036
Size: 4119 1917
MslI CAynn'nnrTG
Cuts at: 0 2414 3016 6036
Size: 2414 602 3020
Fragments arranged by size:
3020 2414 602
MspA1I CmG'CkG
Cuts at: 0 844 1150 5281 5698 5704 6036
Size: 844 306 4131 417 6 332
Fragments arranged by size:
4131 844 417 332 306 6
NdeI CA'TA_TG
Cuts at: 0 1275 1999 6036
Size: 1275 724 4037
Fragments arranged by size:
4037 1275 724
NspI r_CATG'y
Cuts at: 0 1160 4363 5184 6036
Size: 1160 3203 821 852
Fragments arranged by size:
3203 1160 852 821
PasI CC'CwG_GG
Cuts at: 0 2078 6036
Size: 2078 3958
PciI A'CATG_T
Cuts at: 0 1156 6036
Size: 1156 4880
PflMI CCAn_nnn'nTGG
Cuts at: 0 1607 3586 4456 4588 6036
Size: 1607 1979 870 132 1448
Fragments arranged by size:
1979 1607 1448 870 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5180 5212 5610 5642 6036
Size: 5180 32 398 32 394
Fragments arranged by size:
5180 398 394 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 67 6036
Size: 67 5969
PshAI GACnn'nnGTC
Cuts at: 0 1533 6036
Size: 1533 4503
PsiI TTA'TAA
Cuts at: 0 790 6036
Size: 790 5246
PstI C_TGCA'G
Cuts at: 0 1149 5703 6036
Size: 1149 4554 333
Fragments arranged by size:
4554 1149 333
PvuII CAG'CTG
Cuts at: 0 844 1150 5281 5698 5704 6036
Size: 844 306 4131 417 6 332
Fragments arranged by size:
4131 844 417 332 306 6
SacI G_AGCT'C
Cuts at: 0 761 1944 6036
Size: 761 1183 4092
Fragments arranged by size:
4092 1183 761
ScaI AGT'ACT
Cuts at: 0 3969 6036
Size: 3969 2067
SfcI C'TryA_G
Cuts at: 0 1061 1145 1251 2044 3342 3388 4265 4622
Size: 1061 84 106 793 1298 46 877 357
Cuts at: 4622 5699 5861 6036
Size: 1077 162 175
Fragments arranged by size:
1298 1077 1061 877 793 357 175 162
106 84 46
SmaI CCC'GGG
Cuts at: 0 4895 6036
Size: 4895 1141
SmlI C'TyrA_G
Cuts at: 0 477 4775 4882 6036
Size: 477 4298 107 1154
Fragments arranged by size:
4298 1154 477 107
SspI AAT'ATT
Cuts at: 0 654 2998 3072 6036
Size: 654 2344 74 2964
Fragments arranged by size:
2964 2344 654 74
StuI AGG'CCT
Cuts at: 0 2060 5365 5777 6036
Size: 2060 3305 412 259
Fragments arranged by size:
3305 2060 412 259
StyI C'CwwG_G
Cuts at: 0 1547 3436 4587 6036
Size: 1547 1889 1151 1449
Fragments arranged by size:
1889 1547 1449 1151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1310 1847 6036
Size: 1310 537 4189
Fragments arranged by size:
4189 1310 537
TatI w'GTAC_w
Cuts at: 0 200 3967 5823 5992 6036
Size: 200 3767 1856 169 44
Fragments arranged by size:
3767 1856 200 169 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1776 1808 2799 2831 6036
Size: 1776 32 991 32 3205
Fragments arranged by size:
3205 1776 991 32 32
XbaI T'CTAG_A
Cuts at: 0 3465 6036
Size: 3465 2571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1308 3054 3622 4242 6036
Size: 1308 1746 568 620 1794
Fragments arranged by size:
1794 1746 1308 620 568
XmaI C'CCGG_G
Cuts at: 0 4893 6036
Size: 4893 1143
XmnI GAAnn'nnTTC
Cuts at: 0 47 275 2144 5024 5192 6036
Size: 47 228 1869 2880 168 844
Fragments arranged by size:
2880 1869 844 228 168 47
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BclI BglII BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI
MspA1I NdeI NspI PasI PciI PflMI PpiI PpuMI
PshAI PsiI PstI PvuII SacI ScaI SfcI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
ApaLI AscI AsiSI BbeI BcgI BciVI BfrBI BglI
BlpI Bme1580I BmtI BplI BsgI BsiEI BsrBI BssHII
BstEII BstZ17I Bsu36I BtgI CspCI EagI EciI FseI
FspI FspAI HaeII HpaI KasI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XhoI
ZraI