Restriction map for
pF1KA0309
(Linear) (Six-base) MAPSORT of: KIAA0309.seq Check: 6249 from: 1 to: 9147
KIAA0309.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3102 7611 8025 9147
Size: 3102 4509 414 1122
Fragments arranged by size:
4509 3102 1122 414
AatII G_ACGT'C
Cuts at: 0 9027 9147
Size: 9027 120
AccI GT'mk_AC
Cuts at: 0 4363 9147
Size: 4363 4784
Acc65I G'GTAC_C
Cuts at: 0 4398 4629 9147
Size: 4398 231 4518
Fragments arranged by size:
4518 4398 231
AclI AA'CG_TT
Cuts at: 0 1992 9147
Size: 1992 7155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1046 1058 1535 2682 3602 3626 4790
Size: 313 733 12 477 1147 920 24 1164
Cuts at: 4790 5237 6155 6785 7388 7432 7556 8999 9147
Size: 447 918 630 603 44 124 1443 148
Fragments arranged by size:
1443 1164 1147 920 918 733 630 603
477 447 313 148 124 44 24 12
AfeI AGC'GCT
Cuts at: 0 2203 6397 9147
Size: 2203 4194 2750
Fragments arranged by size:
4194 2750 2203
AflII C'TTAA_G
Cuts at: 0 5559 9147
Size: 5559 3588
AflIII A'CryG_T
Cuts at: 0 4053 9147
Size: 4053 5094
AgeI A'CCGG_T
Cuts at: 0 2661 4395 9147
Size: 2661 1734 4752
Fragments arranged by size:
4752 2661 1734
AhdI GACnn_n'nnGTC
Cuts at: 0 669 4019 5361 9147
Size: 669 3350 1342 3786
Fragments arranged by size:
3786 3350 1342 669
AleI CACnn'nnGTG
Cuts at: 0 1960 3565 7786 9147
Size: 1960 1605 4221 1361
Fragments arranged by size:
4221 1960 1605 1361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1298 6377 7804 9147
Size: 1298 5079 1427 1343
Fragments arranged by size:
5079 1427 1343 1298
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5153 5185 6499 6531 9147
Size: 5153 32 1314 32 2616
Fragments arranged by size:
5153 2616 1314 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1306 1426 1702 3019 4309 4497 4650 4692
Size: 1306 120 276 1317 1290 188 153 42
Cuts at: 4692 4818 5851 5873 6844 7222 7336 7435 8074
Size: 126 1033 22 971 378 114 99 639
Cuts at: 8074 8143 8173 9147
Size: 69 30 974
Fragments arranged by size:
1317 1306 1290 1033 974 971 639 378
276 188 153 126 120 114 99 69
42 30 22
ApaI G_GGCC'C
Cuts at: 0 190 480 1376 1655 2992 3104 3865 4741
Size: 190 290 896 279 1337 112 761 876
Cuts at: 4741 4855 5196 7880 8204 8886 9147
Size: 114 341 2684 324 682 261
Fragments arranged by size:
2684 1337 896 876 761 682 341 324
290 279 261 190 114 112
ApoI r'AATT_y
Cuts at: 0 6265 6409 9147
Size: 6265 144 2738
Fragments arranged by size:
6265 2738 144
AvaI C'yCGr_G
Cuts at: 0 1013 1241 3407 3539 4473 5504 5930 6344
Size: 1013 228 2166 132 934 1031 426 414
Cuts at: 6344 6662 7818 7978 8554 9147
Size: 318 1156 160 576 593
Fragments arranged by size:
2166 1156 1031 1013 934 593 576 426
414 318 228 160 132
AvrII C'CTAG_G
Cuts at: 0 6778 9147
Size: 6778 2369
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4952 4985 9147
Size: 4952 33 4162
Fragments arranged by size:
4952 4162 33
BanI G'GyrC_C
Cuts at: 0 127 309 443 1997 2828 2979 3301 3897
Size: 127 182 134 1554 831 151 322 596
Cuts at: 3897 3936 4104 4293 4398 4629 4659 4942 5199
Size: 39 168 189 105 231 30 283 257
Cuts at: 5199 5409 6586 7326 8094 9147
Size: 210 1177 740 768 1053
Fragments arranged by size:
1554 1177 1053 831 768 740 596 322
283 257 231 210 189 182 168 151
134 127 105 39 30
BanII G_rGCy'C
Cuts at: 0 190 320 480 1049 1376 1655 2034 2992
Size: 190 130 160 569 327 279 379 958
Cuts at: 2992 3026 3104 3463 3546 3865 4480 4741 4831
Size: 34 78 359 83 319 615 261 90
Cuts at: 4831 4855 5196 5498 5511 6452 6663 7418 7880
Size: 24 341 302 13 941 211 755 462
Cuts at: 7880 8204 8886 9147
Size: 324 682 261
Fragments arranged by size:
958 941 755 682 615 569 462 379
359 341 327 324 319 302 279 261
261 211 190 160 130 90 83 78
34 24 13
BbeI G_GCGC'C
Cuts at: 0 2983 9147
Size: 2983 6164
BbsI GAAGACnn'nnnn_
Cuts at: 0 1882 2310 3995 6643 9147
Size: 1882 428 1685 2648 2504
Fragments arranged by size:
2648 2504 1882 1685 428
BbvCI CC'TCA_GC
Cuts at: 0 738 953 1187 4386 6326 7340 7425 7544
Size: 738 215 234 3199 1940 1014 85 119
Cuts at: 7544 9147
Size: 1603
Fragments arranged by size:
3199 1940 1603 1014 738 234 215 119
85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 8802 8836 9147
Size: 8802 34 311
Fragments arranged by size:
8802 311 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1167 4980 9147
Size: 1167 3813 4167
Fragments arranged by size:
4167 3813 1167
BglI GCCn_nnn'nGGC
Cuts at: 0 1909 3220 5264 5326 6232 7713 9147
Size: 1909 1311 2044 62 906 1481 1434
Fragments arranged by size:
2044 1909 1481 1434 1311 906 62
BglII A'GATC_T
Cuts at: 0 7206 9147
Size: 7206 1941
BlpI GC'TnA_GC
Cuts at: 0 263 1228 6254 6350 9147
Size: 263 965 5026 96 2797
Fragments arranged by size:
5026 2797 965 263 96
Bme1580I G_kGCm'C
Cuts at: 0 140 190 480 1376 1655 2002 2992 3104
Size: 140 50 290 896 279 347 990 112
Cuts at: 3104 3306 3865 4741 4855 5196 6125 7061 7122
Size: 202 559 876 114 341 929 936 61
Cuts at: 7122 7199 7880 8204 8886 9147
Size: 77 681 324 682 261
Fragments arranged by size:
990 936 929 896 876 682 681 559
347 341 324 290 279 261 202 140
114 112 77 61 50
BmgBI CAC'GTC
Cuts at: 0 5461 9147
Size: 5461 3686
BmrI ACTGGGnnnn_n'
Cuts at: 0 348 1938 1983 2992 3043 4684 4831 5541
Size: 348 1590 45 1009 51 1641 147 710
Cuts at: 5541 6832 6844 8131 8881 9147
Size: 1291 12 1287 750 266
Fragments arranged by size:
1641 1590 1291 1287 1009 750 710 348
266 147 51 45 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2367 6466 8017 8115 9147
Size: 2367 4099 1551 98 1032
Fragments arranged by size:
4099 2367 1551 1032 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 163 567 684 732 1631 1696 2728
Size: 95 68 404 117 48 899 65 1032
Cuts at: 2728 2996 4139 4163 4244 4352 4598 4622 4688
Size: 268 1143 24 81 108 246 24 66
Cuts at: 4688 4706 4715 4754 5143 6118 6199 6436 6448
Size: 18 9 39 389 975 81 237 12
Cuts at: 6448 6779 6983 7121 7168 7432 7480 7494 8048
Size: 331 204 138 47 264 48 14 554
Cuts at: 8048 9147
Size: 1099
Fragments arranged by size:
1143 1099 1032 975 899 554 404 389
331 268 264 246 237 204 138 117
108 95 81 81 68 66 65 48
48 47 39 24 24 18 14 12
9
Bpu10I CC'TnA_GC
Cuts at: 0 386 738 953 1187 2719 2936 3020 4160
Size: 386 352 215 234 1532 217 84 1140
Cuts at: 4160 4386 5894 6326 7340 7425 7544 9147
Size: 226 1508 432 1014 85 119 1603
Fragments arranged by size:
1603 1532 1508 1140 1014 432 386 352
234 226 217 215 119 85 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 613 2035 3565 4845 5605 6659 7116
Size: 71 542 1422 1530 1280 760 1054 457
Cuts at: 7116 9147
Size: 2031
Fragments arranged by size:
2031 1530 1422 1280 1054 760 542 457
71
BsaI GGTCTCn'nnnn_
Cuts at: 0 7803 8066 8146 8793 8837 9147
Size: 7803 263 80 647 44 310
Fragments arranged by size:
7803 647 310 263 80 44
BsaAI yAC'GTr
Cuts at: 0 3663 9147
Size: 3663 5484
BsaBI GATnn'nnATC
Cuts at: 0 2735 9147
Size: 2735 6412
BsaHI Gr'CG_yC
Cuts at: 0 1753 2238 2980 5076 7620 9024 9147
Size: 1753 485 742 2096 2544 1404 123
Fragments arranged by size:
2544 2096 1753 1404 742 485 123
BsaWI w'CCGG_w
Cuts at: 0 1436 2661 4284 4338 4395 9147
Size: 1436 1225 1623 54 57 4752
Fragments arranged by size:
4752 1623 1436 1225 57 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1969 1999 2228 2241 2258 2271 2272 2302
Size: 1969 30 229 13 17 13 1 30
Cuts at: 2302 4752 4782 5805 5835 6499 6529 6945 6975
Size: 2450 30 1023 30 664 30 416 30
Cuts at: 6975 6981 7011 9147
Size: 6 30 2136
Fragments arranged by size:
2450 2136 1969 1023 664 416 229 30
30 30 30 30 30 30 17 13
13 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 502 1279 1385 1483 1573 2206 2999
Size: 466 36 777 106 98 90 633 793
Cuts at: 2999 3644 3705 4256 4259 4268 5989 6154 6349
Size: 645 61 551 3 9 1721 165 195
Cuts at: 6349 6445 6460 6523 6547 6550 6553 6899 6950
Size: 96 15 63 24 3 3 346 51
Cuts at: 6950 7022 7170 7390 7604 8980 9007 9010 9147
Size: 72 148 220 214 1376 27 3 137
Fragments arranged by size:
1721 1376 793 777 645 633 551 466
346 220 214 195 165 148 137 106
98 96 90 72 63 61 51 36
27 24 15 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 42 2002 3032 3441 3804 3864 3909 4083
Size: 42 1960 1030 409 363 60 45 174
Cuts at: 4083 4509 4740 4746 4800 4884 4902 5029 5284
Size: 426 231 6 54 84 18 127 255
Cuts at: 5284 5342 6553 7276 7919 7953 8074 8204 8219
Size: 58 1211 723 643 34 121 130 15
Cuts at: 8219 8512 9147
Size: 293 635
Fragments arranged by size:
1960 1211 1030 723 643 635 426 409
363 293 255 231 174 130 127 121
84 60 58 54 45 42 34 18
15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4831 6242 6936 7256 9147
Size: 4831 1411 694 320 1891
Fragments arranged by size:
4831 1891 1411 694 320
BsiEI CG_ry'CG
Cuts at: 0 2662 4859 9147
Size: 2662 2197 4288
Fragments arranged by size:
4288 2662 2197
BsiHKAI G_wGCw'C
Cuts at: 0 3026 3463 5498 5645 6452 7418 7998 9147
Size: 3026 437 2035 147 807 966 580 1149
Fragments arranged by size:
3026 2035 1149 966 807 580 437 147
BsmI GAATG_Cn'
Cuts at: 0 1847 5672 8687 9147
Size: 1847 3825 3015 460
Fragments arranged by size:
3825 3015 1847 460
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2229 7982 9147
Size: 2229 5753 1165
Fragments arranged by size:
5753 2229 1165
Bsp1286I G_dGCh'C
Cuts at: 0 140 190 320 480 1049 1376 1655 2002
Size: 140 50 130 160 569 327 279 347
Cuts at: 2002 2034 2992 3026 3104 3306 3463 3546 3865
Size: 32 958 34 78 202 157 83 319
Cuts at: 3865 4480 4741 4831 4855 5196 5498 5511 5645
Size: 615 261 90 24 341 302 13 134
Cuts at: 5645 6125 6452 6663 7061 7122 7199 7418 7880
Size: 480 327 211 398 61 77 219 462
Cuts at: 7880 7998 8204 8886 9147
Size: 118 206 682 261
Fragments arranged by size:
958 682 615 569 480 462 398 347
341 327 327 319 302 279 261 261
219 211 206 202 160 157 140 134
130 118 90 83 78 77 61 50
34 32 24 13
BspHI T'CATG_A
Cuts at: 0 999 2591 9147
Size: 999 1592 6556
Fragments arranged by size:
6556 1592 999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 629 3102 5723 7611 8025 9147
Size: 629 2473 2621 1888 414 1122
Fragments arranged by size:
2621 2473 1888 1122 629 414
BsrBI CCG'CTC
Cuts at: 0 4948 9147
Size: 4948 4199
BsrDI GCAATG_nn'
Cuts at: 0 558 870 1688 5011 7410 7931 9147
Size: 558 312 818 3323 2399 521 1216
Fragments arranged by size:
3323 2399 1216 818 558 521 312
BsrFI r'CCGG_y
Cuts at: 0 2661 3509 4395 6314 6593 9038 9147
Size: 2661 848 886 1919 279 2445 109
Fragments arranged by size:
2661 2445 1919 886 848 279 109
BssSI C'ACGA_G
Cuts at: 0 448 6635 8291 9147
Size: 448 6187 1656 856
Fragments arranged by size:
6187 1656 856 448
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4189 4942 5792 6820 9147
Size: 4189 753 850 1028 2327
Fragments arranged by size:
4189 2327 1028 850 753
BstBI TT'CG_AA
Cuts at: 0 7698 9147
Size: 7698 1449
BstEII G'GTnAC_C
Cuts at: 0 1818 2664 4021 9147
Size: 1818 846 1357 5126
Fragments arranged by size:
5126 1818 1357 846
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1801 4215 5447 8212 8715 9147
Size: 1801 2414 1232 2765 503 432
Fragments arranged by size:
2765 2414 1801 1232 503 432
BstYI r'GATC_y
Cuts at: 0 5728 7206 7229 8169 9147
Size: 5728 1478 23 940 978
Fragments arranged by size:
5728 1478 978 940 23
Bsu36I CC'TnA_GG
Cuts at: 0 305 1052 1397 7103 7589 8951 8985 9147
Size: 305 747 345 5706 486 1362 34 162
Fragments arranged by size:
5706 1362 747 486 345 305 162 34
BtgI C'CryG_G
Cuts at: 0 521 1220 1259 1376 2726 2974 3359 4076
Size: 521 699 39 117 1350 248 385 717
Cuts at: 4076 4964 5700 5885 8708 9147
Size: 888 736 185 2823 439
Fragments arranged by size:
2823 1350 888 736 717 699 521 439
385 248 185 117 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2314 4283 9147
Size: 2314 1969 4864
Fragments arranged by size:
4864 2314 1969
BtsI GCAGTG_nn'
Cuts at: 0 370 1320 1638 3151 5042 5609 7903 7996
Size: 370 950 318 1513 1891 567 2294 93
Cuts at: 7996 8395 8411 9147
Size: 399 16 736
Fragments arranged by size:
2294 1891 1513 950 736 567 399 370
318 93 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1894 1929 9147
Size: 1894 35 7218
Fragments arranged by size:
7218 1894 35
DraI TTT'AAA
Cuts at: 0 2010 9147
Size: 2010 7137
DraIII CAC_nnn'GTG
Cuts at: 0 5640 9147
Size: 5640 3507
EaeI y'GGCC_r
Cuts at: 0 524 1733 3507 3983 4121 4856 5703 5922
Size: 524 1209 1774 476 138 735 847 219
Cuts at: 5922 6023 6155 6312 7163 7666 9147
Size: 101 132 157 851 503 1481
Fragments arranged by size:
1774 1481 1209 851 847 735 524 503
476 219 157 138 132 101
EagI C'GGCC_G
Cuts at: 0 4856 9147
Size: 4856 4291
EarI CTCTTCn'nnn_
Cuts at: 0 196 289 350 720 738 812 923 1037
Size: 196 93 61 370 18 74 111 114
Cuts at: 1037 1127 1145 1163 1382 1511 1550 1601 3581
Size: 90 18 18 219 129 39 51 1980
Cuts at: 3581 3654 3696 4053 4286 5998 6131 6140 6203
Size: 73 42 357 233 1712 133 9 63
Cuts at: 6203 6458 7475 8467 8689 9147
Size: 255 1017 992 222 458
Fragments arranged by size:
1980 1712 1017 992 458 370 357 255
233 222 219 196 133 129 114 111
93 90 74 73 63 61 51 42
39 18 18 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6508 6693 9147
Size: 6508 185 2454
Fragments arranged by size:
6508 2454 185
EcoICRI GAG'CTC
Cuts at: 0 3024 3461 5496 6450 7416 9147
Size: 3024 437 2035 954 966 1731
Fragments arranged by size:
3024 2035 1731 966 954 437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 163 313 567 684 732 1046 1058
Size: 95 68 150 254 117 48 314 12
Cuts at: 1058 1535 1631 1696 2682 2728 2996 3602 3626
Size: 477 96 65 986 46 268 606 24
Cuts at: 3626 4139 4163 4244 4352 4598 4622 4688 4706
Size: 513 24 81 108 246 24 66 18
Cuts at: 4706 4715 4754 4790 5143 5237 6118 6155 6199
Size: 9 39 36 353 94 881 37 44
Cuts at: 6199 6436 6448 6779 6785 6983 7121 7168 7388
Size: 237 12 331 6 198 138 47 220
Cuts at: 7388 7432 7480 7494 7556 8048 8999 9147
Size: 44 48 14 62 492 951 148
Fragments arranged by size:
986 951 881 606 513 492 477 353
331 314 268 254 246 237 220 198
150 148 138 117 108 96 95 94
81 68 66 65 62 48 48 47
46 44 44 39 37 36 24 24
24 18 14 12 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1028 1646 2419 3011 5989 9147
Size: 1028 618 773 592 2978 3158
Fragments arranged by size:
3158 2978 1028 773 618 592
EcoO109I rG'GnC_Cy
Cuts at: 0 115 186 967 1372 1651 1933 2989 3016
Size: 115 71 781 405 279 282 1056 27
Cuts at: 3016 3101 4582 5192 5193 7472 7594 7684 7877
Size: 85 1481 610 1 2279 122 90 193
Cuts at: 7877 8042 8201 8882 8883 8947 9011 9147
Size: 165 159 681 1 64 64 136
Fragments arranged by size:
2279 1481 1056 781 681 610 405 282
279 193 165 159 136 122 115 90
85 71 64 64 27 1 1
EcoRV GAT'ATC
Cuts at: 0 6216 9147
Size: 6216 2931
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1270 9147
Size: 1270 7877
FseI GG_CCGG'CC
Cuts at: 0 6318 9147
Size: 6318 2829
FspI TGC'GCA
Cuts at: 0 430 5542 9082 9147
Size: 430 5112 3540 65
Fragments arranged by size:
5112 3540 430 65
FspAI rTGC'GCAy
Cuts at: 0 430 5542 9147
Size: 430 5112 3605
Fragments arranged by size:
5112 3605 430
HaeII r_GCGC'y
Cuts at: 0 396 2154 2205 2983 4879 6399 9092 9147
Size: 396 1758 51 778 1896 1520 2693 55
Fragments arranged by size:
2693 1896 1758 1520 778 396 55 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 655 687 1519 1551 1878 1910
Size: 237 32 386 32 832 32 327 32
Cuts at: 1910 2228 2241 2260 2272 2273 2304 4005 4037
Size: 318 13 19 12 1 31 1701 32
Cuts at: 4037 4680 4712 4752 4784 5347 5379 5630 5662
Size: 643 32 40 32 563 32 251 32
Cuts at: 5662 6325 6357 7459 7491 8323 8355 9147
Size: 663 32 1102 32 832 32 792
Fragments arranged by size:
1701 1102 832 832 792 663 643 563
386 327 318 251 237 40 32 32
32 32 32 32 32 32 32 32
32 32 31 19 13 12 1
HincII GTy'rAC
Cuts at: 0 2524 2913 3500 3875 9147
Size: 2524 389 587 375 5272
Fragments arranged by size:
5272 2524 587 389 375
HindIII A'AGCT_T
Cuts at: 0 1837 9147
Size: 1837 7310
HpaI GTT'AAC
Cuts at: 0 3500 9147
Size: 3500 5647
KasI G'GCGC_C
Cuts at: 0 2979 9147
Size: 2979 6168
KpnI G_GTAC'C
Cuts at: 0 4402 4633 9147
Size: 4402 231 4514
Fragments arranged by size:
4514 4402 231
MfeI C'AATT_G
Cuts at: 0 5482 9147
Size: 5482 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 941 1040 1178 4432 4615 4705 5196
Size: 379 562 99 138 3254 183 90 491
Cuts at: 5196 8011 8329 8747 9147
Size: 2815 318 418 400
Fragments arranged by size:
3254 2815 562 491 418 400 379 318
183 138 99 90
MscI TGG'CCA
Cuts at: 0 526 1735 3985 4123 5705 5924 6025 6157
Size: 526 1209 2250 138 1582 219 101 132
Cuts at: 6157 7165 9147
Size: 1008 1982
Fragments arranged by size:
2250 1982 1582 1209 1008 526 219 138
132 101
MslI CAynn'nnrTG
Cuts at: 0 1004 1118 1960 2611 3565 5656 7786 8713
Size: 1004 114 842 651 954 2091 2130 927
Cuts at: 8713 9147
Size: 434
Fragments arranged by size:
2130 2091 1004 954 927 842 651 434
114
MspA1I CmG'CkG
Cuts at: 0 459 742 957 1186 1702 2504 2619 2748
Size: 459 283 215 229 516 802 115 129
Cuts at: 2748 3342 3475 3913 5145 5361 5909 6381 6740
Size: 594 133 438 1232 216 548 472 359
Cuts at: 6740 7543 7767 9147
Size: 803 224 1380
Fragments arranged by size:
1380 1232 803 802 594 548 516 472
459 438 359 283 229 224 216 215
133 129 115
NaeI GCC'GGC
Cuts at: 0 3511 6316 9040 9147
Size: 3511 2805 2724 107
Fragments arranged by size:
3511 2805 2724 107
NarI GG'CG_CC
Cuts at: 0 2980 9147
Size: 2980 6167
NcoI C'CATG_G
Cuts at: 0 521 1220 1259 3359 4964 5700 5885 8708
Size: 521 699 39 2100 1605 736 185 2823
Cuts at: 8708 9147
Size: 439
Fragments arranged by size:
2823 2100 1605 736 699 521 439 185
39
NgoMIV G'CCGG_C
Cuts at: 0 3509 6314 9038 9147
Size: 3509 2805 2724 109
Fragments arranged by size:
3509 2805 2724 109
NruI TCG'CGA
Cuts at: 0 2333 9147
Size: 2333 6814
NspI r_CATG'y
Cuts at: 0 2497 4057 4091 5431 5541 7845 9147
Size: 2497 1560 34 1340 110 2304 1302
Fragments arranged by size:
2497 2304 1560 1340 1302 110 34
PasI CC'CwG_GG
Cuts at: 0 2985 3679 4578 4848 8143 8308 8589 8943
Size: 2985 694 899 270 3295 165 281 354
Cuts at: 8943 9147
Size: 204
Fragments arranged by size:
3295 2985 899 694 354 281 270 204
165
PciI A'CATG_T
Cuts at: 0 4053 9147
Size: 4053 5094
PflMI CCAn_nnn'nTGG
Cuts at: 0 1306 5300 7128 8143 8893 9147
Size: 1306 3994 1828 1015 750 254
Fragments arranged by size:
3994 1828 1306 1015 750 254
PfoI T'CCnGG_A
Cuts at: 0 1784 3918 4368 5222 5474 6413 8177 9147
Size: 1784 2134 450 854 252 939 1764 970
Fragments arranged by size:
2134 1784 1764 970 939 854 450 252
PmlI CAC'GTG
Cuts at: 0 3663 9147
Size: 3663 5484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6499 6531 9147
Size: 6499 32 2616
Fragments arranged by size:
6499 2616 32
PpuMI rG'GwC_Cy
Cuts at: 0 1933 3016 4582 7472 7594 9011 9147
Size: 1933 1083 1566 2890 122 1417 136
Fragments arranged by size:
2890 1933 1566 1417 1083 136 122
PshAI GACnn'nnGTC
Cuts at: 0 2759 5218 5909 6637 9147
Size: 2759 2459 691 728 2510
Fragments arranged by size:
2759 2510 2459 728 691
PspOMI G'GGCC_C
Cuts at: 0 186 476 1372 1651 2988 3100 3861 4737
Size: 186 290 896 279 1337 112 761 876
Cuts at: 4737 4851 5192 7876 8200 8882 9147
Size: 114 341 2684 324 682 265
Fragments arranged by size:
2684 1337 896 876 761 682 341 324
290 279 265 186 114 112
PspXI vC'TCGA_Gb
Cuts at: 0 3539 9147
Size: 3539 5608
PstI C_TGCA'G
Cuts at: 0 1698 2120 2509 2927 3158 6745 8328 9147
Size: 1698 422 389 418 231 3587 1583 819
Fragments arranged by size:
3587 1698 1583 819 422 418 389 231
PvuII CAG'CTG
Cuts at: 0 742 957 1186 1702 2619 3475 5361 6381
Size: 742 215 229 516 917 856 1886 1020
Cuts at: 6381 7543 9147
Size: 1162 1604
Fragments arranged by size:
1886 1604 1162 1020 917 856 742 516
229 215
SacI G_AGCT'C
Cuts at: 0 3026 3463 5498 6452 7418 9147
Size: 3026 437 2035 954 966 1729
Fragments arranged by size:
3026 2035 1729 966 954 437
SanDI GG'GwC_CC
Cuts at: 0 1933 4582 9011 9147
Size: 1933 2649 4429 136
Fragments arranged by size:
4429 2649 1933 136
SapI GCTCTTCn'nnn_
Cuts at: 0 196 720 738 923 1037 1382 1601 6458
Size: 196 524 18 185 114 345 219 4857
Cuts at: 6458 7475 9147
Size: 1017 1672
Fragments arranged by size:
4857 1672 1017 524 345 219 196 185
114 18
ScaI AGT'ACT
Cuts at: 0 658 1588 9147
Size: 658 930 7559
Fragments arranged by size:
7559 930 658
SexAI A'CCwGG_T
Cuts at: 0 4017 9147
Size: 4017 5130
SfcI C'TryA_G
Cuts at: 0 1178 1694 2116 2505 2923 3154 3743 6741
Size: 1178 516 422 389 418 231 589 2998
Cuts at: 6741 8324 8926 9147
Size: 1583 602 221
Fragments arranged by size:
2998 1583 1178 602 589 516 422 418
389 231 221
SfoI GGC'GCC
Cuts at: 0 2981 9147
Size: 2981 6166
SgrAI Cr'CCGG_yG
Cuts at: 0 6593 9147
Size: 6593 2554
SmaI CCC'GGG
Cuts at: 0 5506 5932 6346 6664 7820 9147
Size: 5506 426 414 318 1156 1327
Fragments arranged by size:
5506 1327 1156 426 414 318
SmlI C'TyrA_G
Cuts at: 0 86 592 1241 2050 3539 3580 4824 5559
Size: 86 506 649 809 1489 41 1244 735
Cuts at: 5559 5620 6674 7095 8554 9147
Size: 61 1054 421 1459 593
Fragments arranged by size:
1489 1459 1244 1054 809 735 649 593
506 421 86 61 41
SpeI A'CTAG_T
Cuts at: 0 7807 9147
Size: 7807 1340
SphI G_CATG'C
Cuts at: 0 4091 5431 9147
Size: 4091 1340 3716
Fragments arranged by size:
4091 3716 1340
SrfI GCCC'GGGC
Cuts at: 0 6664 9147
Size: 6664 2483
StuI AGG'CCT
Cuts at: 0 2131 2433 3573 5755 6421 6783 7101 9147
Size: 2131 302 1140 2182 666 362 318 2046
Fragments arranged by size:
2182 2131 2046 1140 666 362 318 302
StyI C'CwwG_G
Cuts at: 0 521 527 1108 1220 1259 1718 3359 3932
Size: 521 6 581 112 39 459 1641 573
Cuts at: 3932 4871 4964 4988 5446 5700 5885 6236 6503
Size: 939 93 24 458 254 185 351 267
Cuts at: 6503 6778 7088 7286 8595 8708 9060 9147
Size: 275 310 198 1309 113 352 87
Fragments arranged by size:
1641 1309 939 581 573 521 459 458
352 351 310 275 267 254 198 185
113 112 93 87 39 24 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3573 4536 5453 5560 5668 6244 6604 7290
Size: 3573 963 917 107 108 576 360 686
Cuts at: 7290 8384 8478 9147
Size: 1094 94 669
Fragments arranged by size:
3573 1094 963 917 686 669 576 360
108 107 94
TatI w'GTAC_w
Cuts at: 0 656 1586 5331 9147
Size: 656 930 3745 3816
Fragments arranged by size:
3816 3745 930 656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 393 425 3652 3684 3780 3809
Size: 334 32 27 32 3227 32 96 29
Cuts at: 3809 3812 3841 3890 3922 5805 5837 6945 6977
Size: 3 29 49 32 1883 32 1108 32
Cuts at: 6977 9147
Size: 2170
Fragments arranged by size:
3227 2170 1883 1108 334 96 49 32
32 32 32 32 32 29 29 27
3
Tth111I GACn'n_nGTC
Cuts at: 0 4602 4650 4692 8137 9147
Size: 4602 48 42 3445 1010
Fragments arranged by size:
4602 3445 1010 48 42
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 3040 3525 3561 6411 7284 7635 9147
Size: 138 2902 485 36 2850 873 351 1512
Fragments arranged by size:
2902 2850 1512 873 485 351 138 36
XhoI C'TCGA_G
Cuts at: 0 1241 3539 8554 9147
Size: 1241 2298 5015 593
Fragments arranged by size:
5015 2298 1241 593
XmaI C'CCGG_G
Cuts at: 0 5504 5930 6344 6662 7818 9147
Size: 5504 426 414 318 1156 1329
Fragments arranged by size:
5504 1329 1156 426 414 318
XmnI GAAnn'nnTTC
Cuts at: 0 513 9147
Size: 513 8634
ZraI GAC'GTC
Cuts at: 0 9025 9147
Size: 9025 122
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SanDI SapI ScaI SexAI SfcI SfoI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaLI AscI AseI AsiSI BamHI BclI BfrBI BmtI
BsiWI BspEI BsrGI BssHII BstZ17I ClaI DrdI EcoRI
MluI NdeI NheI NotI NsiI PacI PmeI PsiI
PsrI PvuI RsrII SacII SalI SbfI SfiI SgfI
SnaBI SspI SwaI XbaI