Restriction map for
pF1KSDA0313
(Linear) (Six-base) MAPSORT of: KIAA0313.seq Check: 9421 from: 1 to: 4497
KIAA0313.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AatII G_ACGT'C
Cuts at: 0 3751 4497
Size: 3751 746
AccI GT'mk_AC
Cuts at: 0 2663 4497
Size: 2663 1834
Acc65I G'GTAC_C
Cuts at: 0 820 4497
Size: 820 3677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1289 1779 2125 2290 2365 2717 2962 3293
Size: 1289 490 346 165 75 352 245 331
Cuts at: 3293 3329 4279 4497
Size: 36 950 218
Fragments arranged by size:
1289 950 490 352 346 331 245 218
165 75 36
AhdI GACnn_n'nnGTC
Cuts at: 0 516 808 2849 4497
Size: 516 292 2041 1648
Fragments arranged by size:
2041 1648 516 292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3149 3181 4497
Size: 3149 32 1316
Fragments arranged by size:
3149 1316 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3108 3427 4497
Size: 3108 319 1070
Fragments arranged by size:
3108 1070 319
ApoI r'AATT_y
Cuts at: 0 382 1066 1352 2129 2184 2321 2406 2708
Size: 382 684 286 777 55 137 85 302
Cuts at: 2708 3257 3440 4223 4497
Size: 549 183 783 274
Fragments arranged by size:
783 777 684 549 382 302 286 274
183 137 85 55
AvaI C'yCGr_G
Cuts at: 0 966 1175 3965 4497
Size: 966 209 2790 532
Fragments arranged by size:
2790 966 532 209
AvrII C'CTAG_G
Cuts at: 0 3549 4497
Size: 3549 948
BamHI G'GATC_C
Cuts at: 0 3849 4497
Size: 3849 648
BanI G'GyrC_C
Cuts at: 0 820 1450 2448 3512 4497
Size: 820 630 998 1064 985
Fragments arranged by size:
1064 998 985 820 630
BanII G_rGCy'C
Cuts at: 0 4163 4196 4497
Size: 4163 33 301
Fragments arranged by size:
4163 301 33
BbeI G_GCGC'C
Cuts at: 0 3516 4497
Size: 3516 981
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 281 893 2004 2968 3974 4010 4497
Size: 263 18 612 1111 964 1006 36 487
Fragments arranged by size:
1111 1006 964 612 487 263 36 18
BclI T'GATC_A
Cuts at: 0 1845 1868 3591 3903 4497
Size: 1845 23 1723 312 594
Fragments arranged by size:
1845 1723 594 312 23
BfrBI ATG'CAT
Cuts at: 0 843 3328 4497
Size: 843 2485 1169
Fragments arranged by size:
2485 1169 843
BglI GCCn_nnn'nGGC
Cuts at: 0 2917 4362 4497
Size: 2917 1445 135
Fragments arranged by size:
2917 1445 135
BglII A'GATC_T
Cuts at: 0 1509 2514 4497
Size: 1509 1005 1983
Fragments arranged by size:
1983 1509 1005
BlpI GC'TnA_GC
Cuts at: 0 2146 4497
Size: 2146 2351
Bme1580I G_kGCm'C
Cuts at: 0 1455 3206 4253 4497
Size: 1455 1751 1047 244
Fragments arranged by size:
1751 1455 1047 244
BmrI ACTGGGnnnn_n'
Cuts at: 0 2605 3392 3954 4005 4497
Size: 2605 787 562 51 492
Fragments arranged by size:
2605 787 562 492 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 528 4497
Size: 72 456 3969
Fragments arranged by size:
3969 456 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3050 4497
Size: 3050 1447
Bpu10I CC'TnA_GC
Cuts at: 0 1828 2105 2689 4497
Size: 1828 277 584 1808
Fragments arranged by size:
1828 1808 584 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1658 3741 4497
Size: 1658 2083 756
Fragments arranged by size:
2083 1658 756
BsaI GGTCTCn'nnnn_
Cuts at: 0 1968 2801 3052 4497
Size: 1968 833 251 1445
Fragments arranged by size:
1968 1445 833 251
BsaBI GATnn'nnATC
Cuts at: 0 1627 2356 4497
Size: 1627 729 2141
Fragments arranged by size:
2141 1627 729
BsaHI Gr'CG_yC
Cuts at: 0 3513 3748 3979 4497
Size: 3513 235 231 518
Fragments arranged by size:
3513 518 235 231
BsaWI w'CCGG_w
Cuts at: 0 350 4497
Size: 350 4147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 156 186 737 767 3378 3408 4168 4198
Size: 156 30 551 30 2611 30 760 30
Cuts at: 4198 4497
Size: 299
Fragments arranged by size:
2611 760 551 299 156 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2983 3338 4480 4497
Size: 2983 355 1142 17
Fragments arranged by size:
2983 1142 355 17
BseYI C'CCAG_C
Cuts at: 0 18 680 3119 3803 3974 4333 4497
Size: 18 662 2439 684 171 359 164
Fragments arranged by size:
2439 684 662 359 171 164 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2371 3484 4497
Size: 2371 1113 1013
Fragments arranged by size:
2371 1113 1013
BsmI GAATG_Cn'
Cuts at: 0 858 2404 3522 4497
Size: 858 1546 1118 975
Fragments arranged by size:
1546 1118 975 858
Bsp1286I G_dGCh'C
Cuts at: 0 1455 3206 4163 4196 4253 4497
Size: 1455 1751 957 33 57 244
Fragments arranged by size:
1751 1455 957 244 57 33
BspHI T'CATG_A
Cuts at: 0 1865 4497
Size: 1865 2632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3847 4172 4497
Size: 3847 325 325
Fragments arranged by size:
3847 325 325
BsrDI GCAATG_nn'
Cuts at: 0 1052 1723 3016 4163 4497
Size: 1052 671 1293 1147 334
Fragments arranged by size:
1293 1147 1052 671 334
BstAPI GCAn_nnn'nTGC
Cuts at: 0 396 4497
Size: 396 4101
BstEII G'GTnAC_C
Cuts at: 0 111 4497
Size: 111 4386
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1578 2547 4497
Size: 1578 969 1950
Fragments arranged by size:
1950 1578 969
BstYI r'GATC_y
Cuts at: 0 294 535 870 1038 1509 2514 2625 3213
Size: 294 241 335 168 471 1005 111 588
Cuts at: 3213 3849 4497
Size: 636 648
Fragments arranged by size:
1005 648 636 588 471 335 294 241
168 111
Bsu36I CC'TnA_GG
Cuts at: 0 973 1108 1976 4497
Size: 973 135 868 2521
Fragments arranged by size:
2521 973 868 135
BtgI C'CryG_G
Cuts at: 0 27 611 3735 4497
Size: 27 584 3124 762
Fragments arranged by size:
3124 762 584 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1765 4497
Size: 1765 2732
BtsI GCAGTG_nn'
Cuts at: 0 251 467 2383 3001 3496 4497
Size: 251 216 1916 618 495 1001
Fragments arranged by size:
1916 1001 618 495 251 216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 914 949 1877 1912 4497
Size: 914 35 928 35 2585
Fragments arranged by size:
2585 928 914 35 35
DraI TTT'AAA
Cuts at: 0 1410 2238 3251 4497
Size: 1410 828 1013 1246
Fragments arranged by size:
1410 1246 1013 828
DraIII CAC_nnn'GTG
Cuts at: 0 1957 4497
Size: 1957 2540
DrdI GACnn_nn'nnGTC
Cuts at: 0 2671 4497
Size: 2671 1826
EaeI y'GGCC_r
Cuts at: 0 1311 2721 3732 4497
Size: 1311 1410 1011 765
Fragments arranged by size:
1410 1311 1011 765
EarI CTCTTCn'nnn_
Cuts at: 0 497 731 848 1430 2266 2341 3818 4497
Size: 497 234 117 582 836 75 1477 679
Fragments arranged by size:
1477 836 679 582 497 234 117 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2799 3588 4497
Size: 2799 789 909
Fragments arranged by size:
2799 909 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1289 1779 2125 2290 2365 2717 2962 3050
Size: 1289 490 346 165 75 352 245 88
Cuts at: 3050 3293 3329 4279 4497
Size: 243 36 950 218
Fragments arranged by size:
1289 950 490 352 346 245 243 218
165 88 75 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 334 828 4497
Size: 334 494 3669
Fragments arranged by size:
3669 494 334
EcoO109I rG'GnC_Cy
Cuts at: 0 339 3608 4497
Size: 339 3269 889
Fragments arranged by size:
3269 889 339
EcoRI G'AATT_C
Cuts at: 0 4223 4497
Size: 4223 274
EcoRV GAT'ATC
Cuts at: 0 183 4497
Size: 183 4314
HaeII r_GCGC'y
Cuts at: 0 3516 4497
Size: 3516 981
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 156 163 188 274 306 502 534
Size: 131 25 7 25 86 32 196 32
Cuts at: 534 794 826 1214 1246 2109 2141 2835 2867
Size: 260 32 388 32 863 32 694 32
Cuts at: 2867 2973 3005 3304 3336 3694 3726 4497
Size: 106 32 299 32 358 32 771
Fragments arranged by size:
863 771 694 388 358 299 260 196
131 106 86 32 32 32 32 32
32 32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 0 836 899 1163 1239 4497
Size: 836 63 264 76 3258
Fragments arranged by size:
3258 836 264 76 63
HindIII A'AGCT_T
Cuts at: 0 2896 3245 4497
Size: 2896 349 1252
Fragments arranged by size:
2896 1252 349
HpaI GTT'AAC
Cuts at: 0 836 1163 4497
Size: 836 327 3334
Fragments arranged by size:
3334 836 327
KasI G'GCGC_C
Cuts at: 0 3512 4497
Size: 3512 985
KpnI G_GTAC'C
Cuts at: 0 824 4497
Size: 824 3673
MfeI C'AATT_G
Cuts at: 0 344 4497
Size: 344 4153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 4349 4497
Size: 898 3451 148
Fragments arranged by size:
3451 898 148
MscI TGG'CCA
Cuts at: 0 1313 4497
Size: 1313 3184
MslI CAynn'nnrTG
Cuts at: 0 1022 4135 4497
Size: 1022 3113 362
Fragments arranged by size:
3113 1022 362
MspA1I CmG'CkG
Cuts at: 0 1335 2258 2343 4333 4497
Size: 1335 923 85 1990 164
Fragments arranged by size:
1990 1335 923 164 85
NarI GG'CG_CC
Cuts at: 0 3513 4497
Size: 3513 984
NcoI C'CATG_G
Cuts at: 0 27 611 4497
Size: 27 584 3886
Fragments arranged by size:
3886 584 27
NdeI CA'TA_TG
Cuts at: 0 3881 4497
Size: 3881 616
NsiI A_TGCA'T
Cuts at: 0 845 3330 4497
Size: 845 2485 1167
Fragments arranged by size:
2485 1167 845
NspI r_CATG'y
Cuts at: 0 726 4497
Size: 726 3771
PflMI CCAn_nnn'nTGG
Cuts at: 0 3226 4497
Size: 3226 1271
PpuMI rG'GwC_Cy
Cuts at: 0 339 3608 4497
Size: 339 3269 889
Fragments arranged by size:
3269 889 339
PstI C_TGCA'G
Cuts at: 0 69 2996 3858 4497
Size: 69 2927 862 639
Fragments arranged by size:
2927 862 639 69
PvuII CAG'CTG
Cuts at: 0 1335 2258 2343 4333 4497
Size: 1335 923 85 1990 164
Fragments arranged by size:
1990 1335 923 164 85
SapI GCTCTTCn'nnn_
Cuts at: 0 497 848 4497
Size: 497 351 3649
Fragments arranged by size:
3649 497 351
SbfI CC_TGCA'GG
Cuts at: 0 3858 4497
Size: 3858 639
ScaI AGT'ACT
Cuts at: 0 4149 4497
Size: 4149 348
SfcI C'TryA_G
Cuts at: 0 65 903 1517 1883 2391 2992 3160 3647
Size: 65 838 614 366 508 601 168 487
Cuts at: 3647 3653 3854 4497
Size: 6 201 643
Fragments arranged by size:
838 643 614 601 508 487 366 201
168 65 6
SfoI GGC'GCC
Cuts at: 0 3514 4497
Size: 3514 983
SmlI C'TyrA_G
Cuts at: 0 1673 3756 4497
Size: 1673 2083 741
Fragments arranged by size:
2083 1673 741
SpeI A'CTAG_T
Cuts at: 0 151 4497
Size: 151 4346
SspI AAT'ATT
Cuts at: 0 1948 4497
Size: 1948 2549
StuI AGG'CCT
Cuts at: 0 4383 4497
Size: 4383 114
StyI C'CwwG_G
Cuts at: 0 27 611 3076 3373 3549 4449 4497
Size: 27 584 2465 297 176 900 48
Fragments arranged by size:
2465 900 584 297 176 48 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 119 1087 4497
Size: 119 968 3410
Fragments arranged by size:
3410 968 119
TatI w'GTAC_w
Cuts at: 0 2812 4147 4497
Size: 2812 1335 350
Fragments arranged by size:
2812 1335 350
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 4497
Size: 31 4466
Tth111I GACn'n_nGTC
Cuts at: 0 420 4497
Size: 420 4077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3405 4497
Size: 3405 1092
XmnI GAAnn'nnTTC
Cuts at: 0 890 1328 2195 2730 4497
Size: 890 438 867 535 1767
Fragments arranged by size:
1767 890 867 535 438
ZraI GAC'GTC
Cuts at: 0 3749 4497
Size: 3749 748
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AloI AlwNI ApoI
AvaI AvrII BamHI BanI BanII BbeI BbsI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NsiI NspI PflMI PpuMI PstI PvuII
SapI SbfI ScaI SfcI SfoI SmlI SpeI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XmnI
ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AleI AlfI
ApaI ApaLI AscI AseI AsiSI BaeI BbvCI BcgI
BciVI BmgBI BmtI BsaAI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
ClaI EagI EcoICRI FalI FseI FspI FspAI MluI
NaeI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SexAI
SfiI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI
XbaI XhoI XmaI