Restriction map for
pF1KA0322
(Linear) (Six-base) MAPSORT of: KIAA0322.seq Check: 6889 from: 1 to: 4818
KIAA0322.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2950 4818
Size: 2950 1868
AccI GT'mk_AC
Cuts at: 0 4400 4818
Size: 4400 418
Acc65I G'GTAC_C
Cuts at: 0 2244 4818
Size: 2244 2574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1325 3142 4818
Size: 706 619 1817 1676
Fragments arranged by size:
1817 1676 706 619
AfeI AGC'GCT
Cuts at: 0 886 3225 4561 4818
Size: 886 2339 1336 257
Fragments arranged by size:
2339 1336 886 257
AflIII A'CryG_T
Cuts at: 0 2021 3032 4818
Size: 2021 1011 1786
Fragments arranged by size:
2021 1786 1011
AhdI GACnn_n'nnGTC
Cuts at: 0 2280 4818
Size: 2280 2538
AleI CACnn'nnGTG
Cuts at: 0 829 4288 4818
Size: 829 3459 530
Fragments arranged by size:
3459 829 530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4734 4818
Size: 4734 84
AlwNI CAG_nnn'CTG
Cuts at: 0 285 1601 4581 4818
Size: 285 1316 2980 237
Fragments arranged by size:
2980 1316 285 237
ApaI G_GGCC'C
Cuts at: 0 507 4818
Size: 507 4311
ApoI r'AATT_y
Cuts at: 0 1088 3021 4156 4818
Size: 1088 1933 1135 662
Fragments arranged by size:
1933 1135 1088 662
AvaI C'yCGr_G
Cuts at: 0 1657 1858 2881 3062 4404 4818
Size: 1657 201 1023 181 1342 414
Fragments arranged by size:
1657 1342 1023 414 201 181
BanI G'GyrC_C
Cuts at: 0 1149 2244 4456 4818
Size: 1149 1095 2212 362
Fragments arranged by size:
2212 1149 1095 362
BanII G_rGCy'C
Cuts at: 0 507 1322 1457 1799 1859 2266 3657 3824
Size: 507 815 135 342 60 407 1391 167
Cuts at: 3824 3920 4818
Size: 96 898
Fragments arranged by size:
1391 898 815 507 407 342 167 135
96 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 1427 2187 2919 3163 4818
Size: 1427 760 732 244 1655
Fragments arranged by size:
1655 1427 760 732 244
BbvCI CC'TCA_GC
Cuts at: 0 1247 4818
Size: 1247 3571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 83 117 2118 2152 4818
Size: 83 34 2001 34 2666
Fragments arranged by size:
2666 2001 83 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4757 4818
Size: 4757 61
BclI T'GATC_A
Cuts at: 0 611 4818
Size: 611 4207
BglI GCCn_nnn'nGGC
Cuts at: 0 64 4818
Size: 64 4754
BglII A'GATC_T
Cuts at: 0 470 2177 2790 4818
Size: 470 1707 613 2028
Fragments arranged by size:
2028 1707 613 470
BlpI GC'TnA_GC
Cuts at: 0 1798 3414 4818
Size: 1798 1616 1404
Fragments arranged by size:
1798 1616 1404
Bme1580I G_kGCm'C
Cuts at: 0 275 507 2336 4622 4713 4818
Size: 275 232 1829 2286 91 105
Fragments arranged by size:
2286 1829 275 232 105 91
BmgBI CAC'GTC
Cuts at: 0 831 2069 4818
Size: 831 1238 2749
Fragments arranged by size:
2749 1238 831
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 518 1352 2511 2772 4818
Size: 306 212 834 1159 261 2046
Fragments arranged by size:
2046 1159 834 306 261 212
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1992 4818
Size: 1992 2826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1564 1645 1856 1962 2801 3187 3248
Size: 946 618 81 211 106 839 386 61
Cuts at: 3248 3808 4292 4459 4818
Size: 560 484 167 359
Fragments arranged by size:
946 839 618 560 484 386 359 211
167 106 81 61
Bpu10I CC'TnA_GC
Cuts at: 0 1247 2267 4818
Size: 1247 1020 2551
Fragments arranged by size:
2551 1247 1020
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1756 3499 4033 4818
Size: 1756 1743 534 785
Fragments arranged by size:
1756 1743 785 534
BsaI GGTCTCn'nnnn_
Cuts at: 0 574 1271 1296 1654 4818
Size: 574 697 25 358 3164
Fragments arranged by size:
3164 697 574 358 25
BsaAI yAC'GTr
Cuts at: 0 2337 2550 4818
Size: 2337 213 2268
Fragments arranged by size:
2337 2268 213
BsaBI GATnn'nnATC
Cuts at: 0 3500 4523 4818
Size: 3500 1023 295
Fragments arranged by size:
3500 1023 295
BsaHI Gr'CG_yC
Cuts at: 0 67 96 179 4818
Size: 67 29 83 4639
Fragments arranged by size:
4639 83 67 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 1747 1777 2098 2128 2382 2412
Size: 217 30 1500 30 321 30 254 30
Cuts at: 2412 3185 3215 4252 4282 4818
Size: 773 30 1037 30 536
Fragments arranged by size:
1500 1037 773 536 321 254 217 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 765 1066 1411 1465 1477 1519 1534 1537
Size: 765 301 345 54 12 42 15 3
Cuts at: 1537 1753 1756 1770 1771 1774 2043 2148 2190
Size: 216 3 14 1 3 269 105 42
Cuts at: 2190 2230 2233 2236 2750 3272 3874 4289 4818
Size: 40 3 3 514 522 602 415 529
Fragments arranged by size:
765 602 529 522 514 415 345 301
269 216 105 54 42 42 40 15
14 12 3 3 3 3 3 1
BseYI C'CCAG_C
Cuts at: 0 322 1185 1312 1434 1912 1954 2296 2423
Size: 322 863 127 122 478 42 342 127
Cuts at: 2423 2727 2856 3068 4818
Size: 304 129 212 1750
Fragments arranged by size:
1750 863 478 342 322 304 212 129
127 127 122 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 1684 3994 4818
Size: 1382 302 2310 824
Fragments arranged by size:
2310 1382 824 302
BsiEI CG_ry'CG
Cuts at: 0 1209 3168 4035 4818
Size: 1209 1959 867 783
Fragments arranged by size:
1959 1209 867 783
BsiHKAI G_wGCw'C
Cuts at: 0 1322 2191 3824 3920 4818
Size: 1322 869 1633 96 898
Fragments arranged by size:
1633 1322 898 869 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 74 1621 4818
Size: 74 1547 3197
Fragments arranged by size:
3197 1547 74
Bsp1286I G_dGCh'C
Cuts at: 0 275 507 1322 1457 1799 1859 2191 2266
Size: 275 232 815 135 342 60 332 75
Cuts at: 2266 2336 3657 3824 3920 4622 4713 4818
Size: 70 1321 167 96 702 91 105
Fragments arranged by size:
1321 815 702 342 332 275 232 167
135 105 96 91 75 70 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2365 2950 4818
Size: 2365 585 1868
Fragments arranged by size:
2365 1868 585
BsrBI CCG'CTC
Cuts at: 0 114 194 233 4818
Size: 114 80 39 4585
Fragments arranged by size:
4585 114 80 39
BsrDI GCAATG_nn'
Cuts at: 0 2672 3328 4659 4818
Size: 2672 656 1331 159
Fragments arranged by size:
2672 1331 656 159
BsrFI r'CCGG_y
Cuts at: 0 98 4818
Size: 98 4720
BssSI C'ACGA_G
Cuts at: 0 2040 2055 2085 2100 4086 4818
Size: 2040 15 30 15 1986 732
Fragments arranged by size:
2040 1986 732 30 15 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1601 1707 2072 2087 4818
Size: 1601 106 365 15 2731
Fragments arranged by size:
2731 1601 365 106 15
BstBI TT'CG_AA
Cuts at: 0 3306 4818
Size: 3306 1512
BstEII G'GTnAC_C
Cuts at: 0 216 4510 4818
Size: 216 4294 308
Fragments arranged by size:
4294 308 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 2501 4437 4818
Size: 243 2258 1936 381
Fragments arranged by size:
2258 1936 381 243
BstYI r'GATC_y
Cuts at: 0 470 754 2177 2790 3501 4734 4818
Size: 470 284 1423 613 711 1233 84
Fragments arranged by size:
1423 1233 711 613 470 284 84
Bsu36I CC'TnA_GG
Cuts at: 0 157 2449 4818
Size: 157 2292 2369
Fragments arranged by size:
2369 2292 157
BtgI C'CryG_G
Cuts at: 0 62 390 489 641 738 1666 2162 2302
Size: 62 328 99 152 97 928 496 140
Cuts at: 2302 2369 2523 4460 4644 4818
Size: 67 154 1937 184 174
Fragments arranged by size:
1937 928 496 328 184 174 154 152
140 99 97 67 62
BtsI GCAGTG_nn'
Cuts at: 0 1134 1203 1408 4180 4818
Size: 1134 69 205 2772 638
Fragments arranged by size:
2772 1134 638 205 69
ClaI AT'CG_AT
Cuts at: 0 434 2480 4818
Size: 434 2046 2338
Fragments arranged by size:
2338 2046 434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3120 3155 4818
Size: 3120 35 1663
Fragments arranged by size:
3120 1663 35
DraI TTT'AAA
Cuts at: 0 561 4818
Size: 561 4257
DrdI GACnn_nn'nnGTC
Cuts at: 0 599 4818
Size: 599 4219
EaeI y'GGCC_r
Cuts at: 0 56 969 1373 1817 1890 1919 2318 3272
Size: 56 913 404 444 73 29 399 954
Cuts at: 3272 4818
Size: 1546
Fragments arranged by size:
1546 954 913 444 404 399 73 56
29
EarI CTCTTCn'nnn_
Cuts at: 0 1361 1496 1579 1835 1847 2495 3161 3527
Size: 1361 135 83 256 12 648 666 366
Cuts at: 3527 3666 3925 4226 4818
Size: 139 259 301 592
Fragments arranged by size:
1361 666 648 592 366 301 259 256
139 135 83 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1704 2756 4500 4818
Size: 1704 1052 1744 318
Fragments arranged by size:
1744 1704 1052 318
EcoICRI GAG'CTC
Cuts at: 0 1320 3822 3918 4818
Size: 1320 2502 96 900
Fragments arranged by size:
2502 1320 900 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 946 1325 1564 1645 1856 1962 2801
Size: 706 240 379 239 81 211 106 839
Cuts at: 2801 3142 3187 3248 3808 4292 4459 4818
Size: 341 45 61 560 484 167 359
Fragments arranged by size:
839 706 560 484 379 359 341 240
239 211 167 106 81 61 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1327 1393 1546 1840 4818
Size: 1327 66 153 294 2978
Fragments arranged by size:
2978 1327 294 153 66
EcoO109I rG'GnC_Cy
Cuts at: 0 282 503 504 1300 1598 1653 3864 4091
Size: 282 221 1 796 298 55 2211 227
Cuts at: 4091 4818
Size: 727
Fragments arranged by size:
2211 796 727 298 282 227 221 55
1
EcoRI G'AATT_C
Cuts at: 0 4156 4818
Size: 4156 662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 492 906 3674 4818
Size: 492 414 2768 1144
Fragments arranged by size:
2768 1144 492 414
HaeII r_GCGC'y
Cuts at: 0 888 2099 2174 2297 3227 4375 4563 4668
Size: 888 1211 75 123 930 1148 188 105
Cuts at: 4668 4818
Size: 150
Fragments arranged by size:
1211 1148 930 888 188 150 123 105
75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 346 378 427 459 739 746
Size: 217 32 97 32 49 32 280 7
Cuts at: 746 771 778 1007 1039 1063 1095 1387 1419
Size: 25 7 229 32 24 32 292 32
Cuts at: 1419 1747 1779 2266 2298 3185 3217 3472 3504
Size: 328 32 487 32 887 32 255 32
Cuts at: 3504 3526 3558 4252 4284 4818
Size: 22 32 694 32 534
Fragments arranged by size:
887 694 534 487 328 292 280 255
229 217 97 49 32 32 32 32
32 32 32 32 32 32 32 32
25 24 22 7 7
HincII GTy'rAC
Cuts at: 0 4781 4818
Size: 4781 37
HindIII A'AGCT_T
Cuts at: 0 3411 4818
Size: 3411 1407
HpaI GTT'AAC
Cuts at: 0 4781 4818
Size: 4781 37
KpnI G_GTAC'C
Cuts at: 0 2248 4818
Size: 2248 2570
MluI A'CGCG_T
Cuts at: 0 2021 4818
Size: 2021 2797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1006 2602 2994 3758 3834 4818
Size: 583 423 1596 392 764 76 984
Fragments arranged by size:
1596 984 764 583 423 392 76
MscI TGG'CCA
Cuts at: 0 1892 1921 3274 4818
Size: 1892 29 1353 1544
Fragments arranged by size:
1892 1544 1353 29
MslI CAynn'nnrTG
Cuts at: 0 241 829 995 1043 3031 3320 4288 4716
Size: 241 588 166 48 1988 289 968 428
Cuts at: 4716 4818
Size: 102
Fragments arranged by size:
1988 968 588 428 289 241 166 102
48
MspA1I CmG'CkG
Cuts at: 0 1005 1185 1246 1383 1569 1668 1822 2860
Size: 1005 180 61 137 186 99 154 1038
Cuts at: 2860 3596 4462 4538 4818
Size: 736 866 76 280
Fragments arranged by size:
1038 1005 866 736 280 186 180 154
137 99 76 61
NcoI C'CATG_G
Cuts at: 0 62 489 641 738 4818
Size: 62 427 152 97 4080
Fragments arranged by size:
4080 427 152 97 62
NspI r_CATG'y
Cuts at: 0 342 1710 2607 2905 3036 4721 4818
Size: 342 1368 897 298 131 1685 97
Fragments arranged by size:
1685 1368 897 342 298 131 97
PasI CC'CwG_GG
Cuts at: 0 1719 3574 4706 4818
Size: 1719 1855 1132 112
Fragments arranged by size:
1855 1719 1132 112
PciI A'CATG_T
Cuts at: 0 3032 4818
Size: 3032 1786
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 642 1287 2631 4818
Size: 150 492 645 1344 2187
Fragments arranged by size:
2187 1344 645 492 150
PfoI T'CCnGG_A
Cuts at: 0 923 4818
Size: 923 3895
PmlI CAC'GTG
Cuts at: 0 2337 2550 4818
Size: 2337 213 2268
Fragments arranged by size:
2337 2268 213
PpuMI rG'GwC_Cy
Cuts at: 0 282 1300 1653 4818
Size: 282 1018 353 3165
Fragments arranged by size:
3165 1018 353 282
PspOMI G'GGCC_C
Cuts at: 0 503 4818
Size: 503 4315
PspXI vC'TCGA_Gb
Cuts at: 0 4404 4818
Size: 4404 414
PstI C_TGCA'G
Cuts at: 0 1568 2035 2852 3560 4595 4818
Size: 1568 467 817 708 1035 223
Fragments arranged by size:
1568 1035 817 708 467 223
PvuI CG_AT'CG
Cuts at: 0 1209 4818
Size: 1209 3609
PvuII CAG'CTG
Cuts at: 0 1005 1185 1246 1383 1569 4818
Size: 1005 180 61 137 186 3249
Fragments arranged by size:
3249 1005 186 180 137 61
SacI G_AGCT'C
Cuts at: 0 1322 3824 3920 4818
Size: 1322 2502 96 898
Fragments arranged by size:
2502 1322 898 96
SacII CC_GC'GG
Cuts at: 0 1669 4463 4818
Size: 1669 2794 355
Fragments arranged by size:
2794 1669 355
SanDI GG'GwC_CC
Cuts at: 0 1653 4818
Size: 1653 3165
SapI GCTCTTCn'nnn_
Cuts at: 0 1361 1847 3925 4818
Size: 1361 486 2078 893
Fragments arranged by size:
2078 1361 893 486
SbfI CC_TGCA'GG
Cuts at: 0 3560 4818
Size: 3560 1258
ScaI AGT'ACT
Cuts at: 0 3949 4818
Size: 3949 869
SexAI A'CCwGG_T
Cuts at: 0 212 284 4818
Size: 212 72 4534
Fragments arranged by size:
4534 212 72
SfcI C'TryA_G
Cuts at: 0 1564 2031 2046 2061 2076 2091 2106 2848
Size: 1564 467 15 15 15 15 15 742
Cuts at: 2848 3556 3702 3873 4591 4818
Size: 708 146 171 718 227
Fragments arranged by size:
1564 742 718 708 467 227 171 146
15 15 15 15 15
SgrAI Cr'CCGG_yG
Cuts at: 0 98 4818
Size: 98 4720
SmaI CCC'GGG
Cuts at: 0 3064 4818
Size: 3064 1754
SmlI C'TyrA_G
Cuts at: 0 1771 3478 4012 4404 4813 4818
Size: 1771 1707 534 392 409 5
Fragments arranged by size:
1771 1707 534 409 392 5
SphI G_CATG'C
Cuts at: 0 1710 2607 4818
Size: 1710 897 2211
Fragments arranged by size:
2211 1710 897
SspI AAT'ATT
Cuts at: 0 4144 4818
Size: 4144 674
StuI AGG'CCT
Cuts at: 0 53 2454 3235 4818
Size: 53 2401 781 1583
Fragments arranged by size:
2401 1583 781 53
StyI C'CwwG_G
Cuts at: 0 62 489 588 641 738 1445 1457 4818
Size: 62 427 99 53 97 707 12 3361
Fragments arranged by size:
3361 707 427 99 97 62 53 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1055 1130 1177 1381 4381 4818
Size: 1055 75 47 204 3000 437
Fragments arranged by size:
3000 1055 437 204 75 47
TatI w'GTAC_w
Cuts at: 0 3947 4313 4818
Size: 3947 366 505
Fragments arranged by size:
3947 505 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1664 1696 2382 2414 4818
Size: 1664 32 686 32 2404
Fragments arranged by size:
2404 1664 686 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 214 286 4818
Size: 214 72 4532
Fragments arranged by size:
4532 214 72
XbaI T'CTAG_A
Cuts at: 0 76 3247 4818
Size: 76 3171 1571
Fragments arranged by size:
3171 1571 76
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 496 831 1486 2628 4818
Size: 496 335 655 1142 2190
Fragments arranged by size:
2190 1142 655 496 335
XhoI C'TCGA_G
Cuts at: 0 4404 4818
Size: 4404 414
XmaI C'CCGG_G
Cuts at: 0 3062 4818
Size: 3062 1756
XmnI GAAnn'nnTTC
Cuts at: 0 371 4818
Size: 371 4447
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI
CspCI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
HpaI KpnI MluI MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuI PvuII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SgrAI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BfrBI BmtI BsaWI
BsiWI BsmI BspEI BspHI BsrGI BssHII BstZ17I BtgZI
DraIII EagI EcoRV FseI FspI FspAI KasI MfeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PpiI PshAI PsiI PsrI RsrII SalI
SfiI SfoI SgfI SnaBI SpeI SrfI SwaI ZraI