Restriction map for
pF1KSDA0323
(Linear) (Six-base) MAPSORT of: KIAA0323.seq Check: 1809 from: 1 to: 2157
KIAA0323.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1215 2157
Size: 1215 942
Acc65I G'GTAC_C
Cuts at: 0 1845 2157
Size: 1845 312
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 210 352 518 1563 1739 1915 2157
Size: 28 182 142 166 1045 176 176 242
Fragments arranged by size:
1045 242 182 176 176 166 142 28
AlwNI CAG_nnn'CTG
Cuts at: 0 2049 2157
Size: 2049 108
ApaI G_GGCC'C
Cuts at: 0 141 298 2157
Size: 141 157 1859
Fragments arranged by size:
1859 157 141
ApoI r'AATT_y
Cuts at: 0 1888 2157
Size: 1888 269
AvaI C'yCGr_G
Cuts at: 0 633 1136 1334 2018 2157
Size: 633 503 198 684 139
Fragments arranged by size:
684 633 503 198 139
BamHI G'GATC_C
Cuts at: 0 1386 2157
Size: 1386 771
BanI G'GyrC_C
Cuts at: 0 456 465 1102 1191 1330 1845 2157
Size: 456 9 637 89 139 515 312
Fragments arranged by size:
637 515 456 312 139 89 9
BanII G_rGCy'C
Cuts at: 0 141 298 408 763 801 2157
Size: 141 157 110 355 38 1356
Fragments arranged by size:
1356 355 157 141 110 38
BbeI G_GCGC'C
Cuts at: 0 469 1334 2157
Size: 469 865 823
Fragments arranged by size:
865 823 469
BbsI GAAGACnn'nnnn_
Cuts at: 0 1913 2020 2157
Size: 1913 107 137
Fragments arranged by size:
1913 137 107
BbvCI CC'TCA_GC
Cuts at: 0 1633 2157
Size: 1633 524
BclI T'GATC_A
Cuts at: 0 482 2157
Size: 482 1675
BglI GCCn_nnn'nGGC
Cuts at: 0 1572 2157
Size: 1572 585
BlpI GC'TnA_GC
Cuts at: 0 547 762 1931 2157
Size: 547 215 1169 226
Fragments arranged by size:
1169 547 226 215
Bme1580I G_kGCm'C
Cuts at: 0 141 298 461 1107 1196 2157
Size: 141 157 163 646 89 961
Fragments arranged by size:
961 646 163 157 141 89
BmgBI CAC'GTC
Cuts at: 0 443 1266 2157
Size: 443 823 891
Fragments arranged by size:
891 823 443
BmrI ACTGGGnnnn_n'
Cuts at: 0 1870 2157
Size: 1870 287
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1165 2157
Size: 1165 992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 456 617 1138 1465 1479 2071 2157
Size: 310 146 161 521 327 14 592 86
Fragments arranged by size:
592 521 327 310 161 146 86 14
Bpu10I CC'TnA_GC
Cuts at: 0 1633 2157
Size: 1633 524
BsaI GGTCTCn'nnnn_
Cuts at: 0 1229 1247 2157
Size: 1229 18 910
Fragments arranged by size:
1229 910 18
BsaBI GATnn'nnATC
Cuts at: 0 1792 2157
Size: 1792 365
BsaHI Gr'CG_yC
Cuts at: 0 466 712 1331 2157
Size: 466 246 619 826
Fragments arranged by size:
826 619 466 246
BsaWI w'CCGG_w
Cuts at: 0 261 577 1212 2157
Size: 261 316 635 945
Fragments arranged by size:
945 635 316 261
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1477 1507 2157
Size: 1477 30 650
Fragments arranged by size:
1477 650 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 1066 1082 1085 1141 1154 1172 1674
Size: 700 366 16 3 56 13 18 502
Cuts at: 1674 1786 2157
Size: 112 371
Fragments arranged by size:
700 502 371 366 112 56 18 16
13 3
BseYI C'CCAG_C
Cuts at: 0 108 551 771 1195 2157
Size: 108 443 220 424 962
Fragments arranged by size:
962 443 424 220 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 724 1193 1540 2157
Size: 370 354 469 347 617
Fragments arranged by size:
617 469 370 354 347
BsiHKAI G_wGCw'C
Cuts at: 0 442 738 763 780 801 2157
Size: 442 296 25 17 21 1356
Fragments arranged by size:
1356 442 296 25 21 17
BsmI GAATG_Cn'
Cuts at: 0 1179 2006 2157
Size: 1179 827 151
Fragments arranged by size:
1179 827 151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1256 2157
Size: 1256 901
Bsp1286I G_dGCh'C
Cuts at: 0 141 298 408 442 461 738 763 780
Size: 141 157 110 34 19 277 25 17
Cuts at: 780 801 1107 1196 2157
Size: 21 306 89 961
Fragments arranged by size:
961 306 277 157 141 110 89 34
25 21 19 17
BspEI T'CCGG_A
Cuts at: 0 261 577 2157
Size: 261 316 1580
Fragments arranged by size:
1580 316 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1215 2157
Size: 1215 942
BsrBI CCG'CTC
Cuts at: 0 543 2031 2157
Size: 543 1488 126
Fragments arranged by size:
1488 543 126
BsrDI GCAATG_nn'
Cuts at: 0 1505 1511 2157
Size: 1505 6 646
Fragments arranged by size:
1505 646 6
BssSI C'ACGA_G
Cuts at: 0 1658 2157
Size: 1658 499
BstEII G'GTnAC_C
Cuts at: 0 1540 2157
Size: 1540 617
BstXI CCAn_nnnn'nTGG
Cuts at: 0 924 2157
Size: 924 1233
BstYI r'GATC_y
Cuts at: 0 835 1386 1679 2157
Size: 835 551 293 478
Fragments arranged by size:
835 551 478 293
Bsu36I CC'TnA_GG
Cuts at: 0 982 2157
Size: 982 1175
BtgI C'CryG_G
Cuts at: 0 1107 1222 1466 1536 2157
Size: 1107 115 244 70 621
Fragments arranged by size:
1107 621 244 115 70
BtsI GCAGTG_nn'
Cuts at: 0 387 2157
Size: 387 1770
DraIII CAC_nnn'GTG
Cuts at: 0 910 1400 2079 2157
Size: 910 490 679 78
Fragments arranged by size:
910 679 490 78
DrdI GACnn_nn'nnGTC
Cuts at: 0 1555 1917 2157
Size: 1555 362 240
Fragments arranged by size:
1555 362 240
EaeI y'GGCC_r
Cuts at: 0 1419 1463 2157
Size: 1419 44 694
Fragments arranged by size:
1419 694 44
EarI CTCTTCn'nnn_
Cuts at: 0 524 950 1715 1843 1979 2157
Size: 524 426 765 128 136 178
Fragments arranged by size:
765 524 426 178 136 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 771 1423 1783 2157
Size: 771 652 360 374
Fragments arranged by size:
771 652 374 360
EcoICRI GAG'CTC
Cuts at: 0 761 799 2157
Size: 761 38 1358
Fragments arranged by size:
1358 761 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 210 310 352 456 518 617 1138
Size: 28 182 100 42 104 62 99 521
Cuts at: 1138 1465 1479 1563 1739 1915 2071 2157
Size: 327 14 84 176 176 156 86
Fragments arranged by size:
521 327 182 176 176 156 104 100
99 86 84 62 42 28 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 1045 1119 1768 2157
Size: 250 795 74 649 389
Fragments arranged by size:
795 649 389 250 74
EcoO109I rG'GnC_Cy
Cuts at: 0 137 294 295 336 844 1038 1312 1377
Size: 137 157 1 41 508 194 274 65
Cuts at: 1377 1761 2136 2157
Size: 384 375 21
Fragments arranged by size:
508 384 375 274 194 157 137 65
41 21 1
HaeII r_GCGC'y
Cuts at: 0 469 1334 1578 2157
Size: 469 865 244 579
Fragments arranged by size:
865 579 469 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 2157
Size: 820 32 1305
Fragments arranged by size:
1305 820 32
KasI G'GCGC_C
Cuts at: 0 465 1330 2157
Size: 465 865 827
Fragments arranged by size:
865 827 465
KpnI G_GTAC'C
Cuts at: 0 1849 2157
Size: 1849 308
MscI TGG'CCA
Cuts at: 0 1421 1465 2157
Size: 1421 44 692
Fragments arranged by size:
1421 692 44
MslI CAynn'nnrTG
Cuts at: 0 1516 2157
Size: 1516 641
MspA1I CmG'CkG
Cuts at: 0 288 1158 2046 2097 2157
Size: 288 870 888 51 60
Fragments arranged by size:
888 870 288 60 51
NarI GG'CG_CC
Cuts at: 0 466 1331 2157
Size: 466 865 826
Fragments arranged by size:
865 826 466
NcoI C'CATG_G
Cuts at: 0 1107 1222 1466 2157
Size: 1107 115 244 691
Fragments arranged by size:
1107 691 244 115
PasI CC'CwG_GG
Cuts at: 0 408 461 917 1041 2157
Size: 408 53 456 124 1116
Fragments arranged by size:
1116 456 408 124 53
PfoI T'CCnGG_A
Cuts at: 0 680 1757 2157
Size: 680 1077 400
Fragments arranged by size:
1077 680 400
PpuMI rG'GwC_Cy
Cuts at: 0 844 1761 2157
Size: 844 917 396
Fragments arranged by size:
917 844 396
PshAI GACnn'nnGTC
Cuts at: 0 1538 2157
Size: 1538 619
PspOMI G'GGCC_C
Cuts at: 0 137 294 2157
Size: 137 157 1863
Fragments arranged by size:
1863 157 137
PstI C_TGCA'G
Cuts at: 0 35 287 346 359 629 1385 2096 2157
Size: 35 252 59 13 270 756 711 61
Fragments arranged by size:
756 711 270 252 61 59 35 13
PvuII CAG'CTG
Cuts at: 0 288 2046 2157
Size: 288 1758 111
Fragments arranged by size:
1758 288 111
SacI G_AGCT'C
Cuts at: 0 763 801 2157
Size: 763 38 1356
Fragments arranged by size:
1356 763 38
SanDI GG'GwC_CC
Cuts at: 0 844 2157
Size: 844 1313
SapI GCTCTTCn'nnn_
Cuts at: 0 524 2157
Size: 524 1633
SbfI CC_TGCA'GG
Cuts at: 0 1385 2157
Size: 1385 772
ScaI AGT'ACT
Cuts at: 0 1525 2157
Size: 1525 632
SfcI C'TryA_G
Cuts at: 0 31 283 342 355 625 1381 2092 2157
Size: 31 252 59 13 270 756 711 65
Fragments arranged by size:
756 711 270 252 65 59 31 13
SfoI GGC'GCC
Cuts at: 0 467 1332 2157
Size: 467 865 825
Fragments arranged by size:
865 825 467
SmaI CCC'GGG
Cuts at: 0 635 2020 2157
Size: 635 1385 137
Fragments arranged by size:
1385 635 137
StyI C'CwwG_G
Cuts at: 0 299 320 786 1107 1222 1466 2157
Size: 299 21 466 321 115 244 691
Fragments arranged by size:
691 466 321 299 244 115 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 988 2157
Size: 763 225 1169
Fragments arranged by size:
1169 763 225
TatI w'GTAC_w
Cuts at: 0 592 1523 2157
Size: 592 931 634
Fragments arranged by size:
931 634 592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 894 919 926 946 951 978 1477 1509
Size: 894 25 7 20 5 27 499 32
Cuts at: 1509 2157
Size: 648
Fragments arranged by size:
894 648 499 32 27 25 20 7
5
XmaI C'CCGG_G
Cuts at: 0 633 2018 2157
Size: 633 1385 139
Fragments arranged by size:
1385 633 139
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BclI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI KpnI MscI MslI MspA1I NarI NcoI PasI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SapI SbfI ScaI SfcI SfoI SmaI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BfrBI BglII BmtI BpuEI BsaAI
BsiEI BsiWI BspHI BsrFI BsrGI BssHII BstAPI BstBI
BstZ17I BtgZI ClaI CspCI DraI EagI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI