Restriction map for
pF1KA0327
(Linear) (Six-base) MAPSORT of: KIAA0327.seq Check: 3528 from: 1 to: 2460
KIAA0327.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 2045 2460
Size: 2045 415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2053 2460
Size: 2053 407
AflIII A'CryG_T
Cuts at: 0 1156 1634 2460
Size: 1156 478 826
Fragments arranged by size:
1156 826 478
AgeI A'CCGG_T
Cuts at: 0 1531 2460
Size: 1531 929
AleI CACnn'nnGTG
Cuts at: 0 1720 2460
Size: 1720 740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 239 796 2460
Size: 239 557 1664
Fragments arranged by size:
1664 557 239
AlwNI CAG_nnn'CTG
Cuts at: 0 469 1590 2092 2460
Size: 469 1121 502 368
Fragments arranged by size:
1121 502 469 368
ApoI r'AATT_y
Cuts at: 0 394 838 1074 2460
Size: 394 444 236 1386
Fragments arranged by size:
1386 444 394 236
AseI AT'TA_AT
Cuts at: 0 851 2460
Size: 851 1609
AvaI C'yCGr_G
Cuts at: 0 1084 1678 1791 2460
Size: 1084 594 113 669
Fragments arranged by size:
1084 669 594 113
BanI G'GyrC_C
Cuts at: 0 1494 1731 2460
Size: 1494 237 729
Fragments arranged by size:
1494 729 237
BanII G_rGCy'C
Cuts at: 0 284 298 1893 2141 2460
Size: 284 14 1595 248 319
Fragments arranged by size:
1595 319 284 248 14
BbeI G_GCGC'C
Cuts at: 0 1498 1735 2460
Size: 1498 237 725
Fragments arranged by size:
1498 725 237
BbsI GAAGACnn'nnnn_
Cuts at: 0 2100 2460
Size: 2100 360
BbvCI CC'TCA_GC
Cuts at: 0 1624 2146 2460
Size: 1624 522 314
Fragments arranged by size:
1624 522 314
BciVI GTATCCnnnnn_n'
Cuts at: 0 693 2460
Size: 693 1767
BclI T'GATC_A
Cuts at: 0 251 1040 2412 2460
Size: 251 789 1372 48
Fragments arranged by size:
1372 789 251 48
BglII A'GATC_T
Cuts at: 0 408 1275 2460
Size: 408 867 1185
Fragments arranged by size:
1185 867 408
BlpI GC'TnA_GC
Cuts at: 0 514 2460
Size: 514 1946
Bme1580I G_kGCm'C
Cuts at: 0 978 2460
Size: 978 1482
BmgBI CAC'GTC
Cuts at: 0 543 2460
Size: 543 1917
BmrI ACTGGGnnnn_n'
Cuts at: 0 902 1057 2460
Size: 902 155 1403
Fragments arranged by size:
1403 902 155
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1927 2460
Size: 1927 533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 464 484 572 607 2158 2460
Size: 186 278 20 88 35 1551 302
Fragments arranged by size:
1551 302 278 186 88 35 20
Bpu10I CC'TnA_GC
Cuts at: 0 1624 2140 2146 2460
Size: 1624 516 6 314
Fragments arranged by size:
1624 516 314 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1125 1810 1897 2460
Size: 775 350 685 87 563
Fragments arranged by size:
775 685 563 350 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 1571 2460
Size: 1571 889
BsaAI yAC'GTr
Cuts at: 0 687 1159 1323 2460
Size: 687 472 164 1137
Fragments arranged by size:
1137 687 472 164
BsaHI Gr'CG_yC
Cuts at: 0 1495 1732 2460
Size: 1495 237 728
Fragments arranged by size:
1495 728 237
BsaWI w'CCGG_w
Cuts at: 0 842 1531 2182 2460
Size: 842 689 651 278
Fragments arranged by size:
842 689 651 278
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 517 547 2156 2186 2460
Size: 109 30 378 30 1609 30 274
Fragments arranged by size:
1609 378 274 109 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 1415 2245 2460
Size: 292 1123 830 215
Fragments arranged by size:
1123 830 292 215
BseYI C'CCAG_C
Cuts at: 0 57 2312 2460
Size: 57 2255 148
Fragments arranged by size:
2255 148 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1303 1409 1846 2460
Size: 565 738 106 437 614
Fragments arranged by size:
738 614 565 437 106
BsiEI CG_ry'CG
Cuts at: 0 2045 2460
Size: 2045 415
BsiHKAI G_wGCw'C
Cuts at: 0 284 450 2460
Size: 284 166 2010
Fragments arranged by size:
2010 284 166
BsmI GAATG_Cn'
Cuts at: 0 1455 2460
Size: 1455 1005
BsmBI CGTCTCn'nnnn_
Cuts at: 0 106 204 1898 2460
Size: 106 98 1694 562
Fragments arranged by size:
1694 562 106 98
Bsp1286I G_dGCh'C
Cuts at: 0 284 298 450 978 1893 2141 2460
Size: 284 14 152 528 915 248 319
Fragments arranged by size:
915 528 319 284 248 152 14
BspEI T'CCGG_A
Cuts at: 0 842 2460
Size: 842 1618
BspHI T'CATG_A
Cuts at: 0 2391 2460
Size: 2391 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1589 2460
Size: 1589 871
BsrBI CCG'CTC
Cuts at: 0 7 1739 2460
Size: 7 1732 721
Fragments arranged by size:
1732 721 7
BsrFI r'CCGG_y
Cuts at: 0 1531 2460
Size: 1531 929
BssHII G'CGCG_C
Cuts at: 0 50 591 2460
Size: 50 541 1869
Fragments arranged by size:
1869 541 50
BssSI C'ACGA_G
Cuts at: 0 1924 2077 2460
Size: 1924 153 383
Fragments arranged by size:
1924 383 153
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2092 2460
Size: 2092 368
BstEII G'GTnAC_C
Cuts at: 0 1761 2460
Size: 1761 699
BstXI CCAn_nnnn'nTGG
Cuts at: 0 153 1126 1722 1773 1945 2460
Size: 153 973 596 51 172 515
Fragments arranged by size:
973 596 515 172 153 51
BstYI r'GATC_y
Cuts at: 0 408 1275 1571 1682 2460
Size: 408 867 296 111 778
Fragments arranged by size:
867 778 408 296 111
BtgI C'CryG_G
Cuts at: 0 257 298 1944 1988 2460
Size: 257 41 1646 44 472
Fragments arranged by size:
1646 472 257 44 41
BtsI GCAGTG_nn'
Cuts at: 0 2093 2460
Size: 2093 367
DraI TTT'AAA
Cuts at: 0 1176 2460
Size: 1176 1284
DrdI GACnn_nn'nnGTC
Cuts at: 0 1534 2460
Size: 1534 926
EaeI y'GGCC_r
Cuts at: 0 2 1931 1947 2460
Size: 2 1929 16 513
Fragments arranged by size:
1929 513 16 2
EarI CTCTTCn'nnn_
Cuts at: 0 104 605 1085 1852 2287 2460
Size: 104 501 480 767 435 173
Fragments arranged by size:
767 501 480 435 173 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1953 2460
Size: 1953 507
EcoICRI GAG'CTC
Cuts at: 0 282 2460
Size: 282 2178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 464 484 572 607 2053 2158 2460
Size: 186 278 20 88 35 1446 105 302
Fragments arranged by size:
1446 302 278 186 105 88 35 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 1825 2173 2460
Size: 328 1497 348 287
Fragments arranged by size:
1497 348 328 287
EcoO109I rG'GnC_Cy
Cuts at: 0 150 1614 2460
Size: 150 1464 846
Fragments arranged by size:
1464 846 150
FspI TGC'GCA
Cuts at: 0 679 1879 2460
Size: 679 1200 581
Fragments arranged by size:
1200 679 581
FspAI rTGC'GCAy
Cuts at: 0 679 1879 2460
Size: 679 1200 581
Fragments arranged by size:
1200 679 581
HaeII r_GCGC'y
Cuts at: 0 1498 1735 2154 2460
Size: 1498 237 419 306
Fragments arranged by size:
1498 419 306 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 179 211 734 766 1108 1140
Size: 109 32 38 32 523 32 342 32
Cuts at: 1140 1226 1258 1394 1426 1774 1806 2460
Size: 86 32 136 32 348 32 654
Fragments arranged by size:
654 523 348 342 136 109 86 38
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 474 2046 2375 2460
Size: 474 1572 329 85
Fragments arranged by size:
1572 474 329 85
HpaI GTT'AAC
Cuts at: 0 2375 2460
Size: 2375 85
KasI G'GCGC_C
Cuts at: 0 1494 1731 2460
Size: 1494 237 729
Fragments arranged by size:
1494 729 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 2460
Size: 677 1783
MscI TGG'CCA
Cuts at: 0 1949 2460
Size: 1949 511
MslI CAynn'nnrTG
Cuts at: 0 685 1006 1264 1720 2460
Size: 685 321 258 456 740
Fragments arranged by size:
740 685 456 321 258
MspA1I CmG'CkG
Cuts at: 0 242 259 300 1610 2460
Size: 242 17 41 1310 850
Fragments arranged by size:
1310 850 242 41 17
NarI GG'CG_CC
Cuts at: 0 1495 1732 2460
Size: 1495 237 728
Fragments arranged by size:
1495 728 237
NcoI C'CATG_G
Cuts at: 0 1944 2460
Size: 1944 516
NspI r_CATG'y
Cuts at: 0 765 1638 2334 2460
Size: 765 873 696 126
Fragments arranged by size:
873 765 696 126
PciI A'CATG_T
Cuts at: 0 1634 2460
Size: 1634 826
PfoI T'CCnGG_A
Cuts at: 0 441 1937 2174 2460
Size: 441 1496 237 286
Fragments arranged by size:
1496 441 286 237
PmlI CAC'GTG
Cuts at: 0 687 1159 1323 2460
Size: 687 472 164 1137
Fragments arranged by size:
1137 687 472 164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1275 1307 2460
Size: 1275 32 1153
Fragments arranged by size:
1275 1153 32
PpuMI rG'GwC_Cy
Cuts at: 0 150 1614 2460
Size: 150 1464 846
Fragments arranged by size:
1464 846 150
PstI C_TGCA'G
Cuts at: 0 1490 1586 2252 2460
Size: 1490 96 666 208
Fragments arranged by size:
1490 666 208 96
SacI G_AGCT'C
Cuts at: 0 284 2460
Size: 284 2176
SacII CC_GC'GG
Cuts at: 0 260 301 2460
Size: 260 41 2159
Fragments arranged by size:
2159 260 41
SalI G'TCGA_C
Cuts at: 0 2044 2460
Size: 2044 416
SanDI GG'GwC_CC
Cuts at: 0 1614 2460
Size: 1614 846
SexAI A'CCwGG_T
Cuts at: 0 626 1757 2460
Size: 626 1131 703
Fragments arranged by size:
1131 703 626
SfcI C'TryA_G
Cuts at: 0 1486 1582 2248 2460
Size: 1486 96 666 212
Fragments arranged by size:
1486 666 212 96
SfoI GGC'GCC
Cuts at: 0 1496 1733 2460
Size: 1496 237 727
Fragments arranged by size:
1496 727 237
SgrAI Cr'CCGG_yG
Cuts at: 0 1531 2460
Size: 1531 929
SmaI CCC'GGG
Cuts at: 0 1086 2460
Size: 1086 1374
SmlI C'TyrA_G
Cuts at: 0 754 1104 1825 1912 2460
Size: 754 350 721 87 548
Fragments arranged by size:
754 721 548 350 87
SspI AAT'ATT
Cuts at: 0 315 1225 2460
Size: 315 910 1235
Fragments arranged by size:
1235 910 315
StyI C'CwwG_G
Cuts at: 0 146 1766 1944 2460
Size: 146 1620 178 516
Fragments arranged by size:
1620 516 178 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1247 1489 2031 2032 2212 2460
Size: 1247 242 542 1 180 248
Fragments arranged by size:
1247 542 248 242 180 1
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 2156 2188 2460
Size: 517 32 1607 32 272
Fragments arranged by size:
1607 517 272 32 32
XbaI T'CTAG_A
Cuts at: 0 411 918 2460
Size: 411 507 1542
Fragments arranged by size:
1542 507 411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2182 2460
Size: 2182 278
XmaI C'CCGG_G
Cuts at: 0 1084 2460
Size: 1084 1376
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlfI AlwNI ApoI
AseI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI BtsI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII
Hin4I HincII HpaI KasI MmeI MscI MslI MspA1I
NarI NcoI NspI PciI PfoI PmlI PpiI PpuMI
PstI SacI SacII SalI SanDI SexAI SfcI SfoI
SgrAI SmaI SmlI SspI StyI TaqII TstI XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BcgI
BfrBI BglI BmtI BsaBI BsiWI BsrDI BsrGI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI EcoRI
EcoRV FalI FseI HindIII KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SapI SbfI ScaI SfiI SgfI SnaBI
SpeI SphI SrfI StuI SwaI TatI Tth111I XhoI
XmnI ZraI