Restriction map for
pF1KSDA0332
(Linear) (Six-base) MAPSORT of: KIAA0332.seq Check: 1563 from: 1 to: 3087
KIAA0332.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AccI GT'mk_AC
Cuts at: 0 2823 3087
Size: 2823 264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1721 1730 1799 1944 2252 2453 3087
Size: 62 1659 9 69 145 308 201 634
Fragments arranged by size:
1659 634 308 201 145 69 62 9
AflII C'TTAA_G
Cuts at: 0 1600 3087
Size: 1600 1487
AlwNI CAG_nnn'CTG
Cuts at: 0 340 712 3087
Size: 340 372 2375
Fragments arranged by size:
2375 372 340
ApaLI G'TGCA_C
Cuts at: 0 2114 3087
Size: 2114 973
ApoI r'AATT_y
Cuts at: 0 638 877 1008 1275 1849 2138 3087
Size: 638 239 131 267 574 289 949
Fragments arranged by size:
949 638 574 289 267 239 131
AseI AT'TA_AT
Cuts at: 0 842 3087
Size: 842 2245
BanI G'GyrC_C
Cuts at: 0 2086 3087
Size: 2086 1001
BanII G_rGCy'C
Cuts at: 0 148 2546 2893 3087
Size: 148 2398 347 194
Fragments arranged by size:
2398 347 194 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 512 1234 2240 3087
Size: 512 722 1006 847
Fragments arranged by size:
1006 847 722 512
BclI T'GATC_A
Cuts at: 0 2003 3087
Size: 2003 1084
BfrBI ATG'CAT
Cuts at: 0 97 3087
Size: 97 2990
BglII A'GATC_T
Cuts at: 0 2875 3001 3043 3087
Size: 2875 126 42 44
Fragments arranged by size:
2875 126 44 42
BlpI GC'TnA_GC
Cuts at: 0 275 3087
Size: 275 2812
Bme1580I G_kGCm'C
Cuts at: 0 180 2091 2118 3087
Size: 180 1911 27 969
Fragments arranged by size:
1911 969 180 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 3030 3087
Size: 3030 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 3087
Size: 201 2886
BsaI GGTCTCn'nnnn_
Cuts at: 0 3062 3087
Size: 3062 25
BsaAI yAC'GTr
Cuts at: 0 1318 3087
Size: 1318 1769
BsaBI GATnn'nnATC
Cuts at: 0 2201 2955 3087
Size: 2201 754 132
Fragments arranged by size:
2201 754 132
BsaWI w'CCGG_w
Cuts at: 0 1135 3087
Size: 1135 1952
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 367 689 3087
Size: 191 176 322 2398
Fragments arranged by size:
2398 322 191 176
BseYI C'CCAG_C
Cuts at: 0 2797 3087
Size: 2797 290
BsiHKAI G_wGCw'C
Cuts at: 0 1599 2118 2546 2893 3087
Size: 1599 519 428 347 194
Fragments arranged by size:
1599 519 428 347 194
BsmI GAATG_Cn'
Cuts at: 0 247 3087
Size: 247 2840
Bsp1286I G_dGCh'C
Cuts at: 0 148 180 1599 2091 2118 2546 2893 3087
Size: 148 32 1419 492 27 428 347 194
Fragments arranged by size:
1419 492 428 347 194 148 32 27
BspEI T'CCGG_A
Cuts at: 0 1135 3087
Size: 1135 1952
BsrBI CCG'CTC
Cuts at: 0 1167 3087
Size: 1167 1920
BsrDI GCAATG_nn'
Cuts at: 0 1682 3087
Size: 1682 1405
BsrGI T'GTAC_A
Cuts at: 0 1805 3087
Size: 1805 1282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 880 1463 2279 3087
Size: 880 583 816 808
Fragments arranged by size:
880 816 808 583
BstYI r'GATC_y
Cuts at: 0 22 73 807 1498 2875 3001 3031 3043
Size: 22 51 734 691 1377 126 30 12
Cuts at: 3043 3087
Size: 44
Fragments arranged by size:
1377 734 691 126 51 44 30 22
12
BtsI GCAGTG_nn'
Cuts at: 0 2823 3087
Size: 2823 264
ClaI AT'CG_AT
Cuts at: 0 2162 3087
Size: 2162 925
DraI TTT'AAA
Cuts at: 0 1004 2262 3087
Size: 1004 1258 825
Fragments arranged by size:
1258 1004 825
EarI CTCTTCn'nnn_
Cuts at: 0 230 548 622 978 1544 1818 2096 2770
Size: 230 318 74 356 566 274 278 674
Cuts at: 2770 3087
Size: 317
Fragments arranged by size:
674 566 356 318 317 278 274 230
74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 35 1085 1482 3087
Size: 19 16 1050 397 1605
Fragments arranged by size:
1605 1050 397 19 16
EcoICRI GAG'CTC
Cuts at: 0 2544 2891 3087
Size: 2544 347 196
Fragments arranged by size:
2544 347 196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1721 1730 1799 1944 2252 2453 3087
Size: 62 1659 9 69 145 308 201 634
Fragments arranged by size:
1659 634 308 201 145 69 62 9
EcoRI G'AATT_C
Cuts at: 0 2138 3087
Size: 2138 949
FspI TGC'GCA
Cuts at: 0 1154 3087
Size: 1154 1933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 452 484 1726 1758 3087
Size: 452 32 1242 32 1329
Fragments arranged by size:
1329 1242 452 32 32
HindIII A'AGCT_T
Cuts at: 0 1426 1565 3087
Size: 1426 139 1522
Fragments arranged by size:
1522 1426 139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1480 2782 3087
Size: 109 1371 1302 305
Fragments arranged by size:
1371 1302 305 109
MslI CAynn'nnrTG
Cuts at: 0 92 2277 3087
Size: 92 2185 810
Fragments arranged by size:
2185 810 92
MspA1I CmG'CkG
Cuts at: 0 337 3087
Size: 337 2750
NdeI CA'TA_TG
Cuts at: 0 89 3087
Size: 89 2998
NsiI A_TGCA'T
Cuts at: 0 99 3087
Size: 99 2988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2470 2502 3087
Size: 2470 32 585
Fragments arranged by size:
2470 585 32
PstI C_TGCA'G
Cuts at: 0 1445 3087
Size: 1445 1642
PvuII CAG'CTG
Cuts at: 0 337 3087
Size: 337 2750
SacI G_AGCT'C
Cuts at: 0 2546 2893 3087
Size: 2546 347 194
Fragments arranged by size:
2546 347 194
SapI GCTCTTCn'nnn_
Cuts at: 0 1544 2096 3087
Size: 1544 552 991
Fragments arranged by size:
1544 991 552
ScaI AGT'ACT
Cuts at: 0 2497 3087
Size: 2497 590
SfcI C'TryA_G
Cuts at: 0 1416 1441 2631 3087
Size: 1416 25 1190 456
Fragments arranged by size:
1416 1190 456 25
SmlI C'TyrA_G
Cuts at: 0 216 1600 3087
Size: 216 1384 1487
Fragments arranged by size:
1487 1384 216
SspI AAT'ATT
Cuts at: 0 2019 2040 3087
Size: 2019 21 1047
Fragments arranged by size:
2019 1047 21
StyI C'CwwG_G
Cuts at: 0 831 1651 3087
Size: 831 820 1436
Fragments arranged by size:
1436 831 820
TatI w'GTAC_w
Cuts at: 0 268 1805 2447 2495 2752 2788 3087
Size: 268 1537 642 48 257 36 299
Fragments arranged by size:
1537 642 299 268 257 48 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 159 191 2776 2808 3087
Size: 159 32 2585 32 279
Fragments arranged by size:
2585 279 159 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1233 2478 3087
Size: 1233 1245 609
Fragments arranged by size:
1245 1233 609
XbaI T'CTAG_A
Cuts at: 0 2998 3087
Size: 2998 89
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1251 1785 3087
Size: 1251 534 1302
Fragments arranged by size:
1302 1251 534
XmnI GAAnn'nnTTC
Cuts at: 0 1478 3087
Size: 1478 1609
Enzymes that do cut:
AccI AcuI AflII AlwNI ApaLI ApoI AseI BanI
BanII BbsI BclI BfrBI BglII BlpI Bme1580I BmrI
BpuEI BsaI BsaAI BsaBI BsaWI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspEI BsrBI BsrDI BsrGI BstXI BstYI
BtsI ClaI DraI EarI EciI EcoICRI Eco57MI EcoRI
FspI Hin4I HindIII MmeI MslI MspA1I NdeI NsiI
PpiI PstI PvuII SacI SapI ScaI SfcI SmlI
SspI StyI TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BglI BmgBI
BmtI BplI BpmI Bpu10I BsaHI BsaXI BsgI BsiEI
BsiWI BsmBI BspHI BspMI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI CspCI DraIII
DrdI EaeI EagI EcoNI EcoO109I EcoRV FalI FseI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII XhoI XmaI ZraI