Restriction map for
pF1KA0333
(Linear) (Six-base) MAPSORT of: KIAA0333.seq Check: 2735 from: 1 to: 3567
KIAA0333.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1860 3567
Size: 1860 1707
AatII G_ACGT'C
Cuts at: 0 2495 3567
Size: 2495 1072
Acc65I G'GTAC_C
Cuts at: 0 1926 3567
Size: 1926 1641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1808 2215 2290 2481 2744 3089 3567
Size: 475 1333 407 75 191 263 345 478
Fragments arranged by size:
1333 478 475 407 345 263 191 75
AfeI AGC'GCT
Cuts at: 0 351 3567
Size: 351 3216
AhdI GACnn_n'nnGTC
Cuts at: 0 1605 3567
Size: 1605 1962
AleI CACnn'nnGTG
Cuts at: 0 391 2932 3278 3567
Size: 391 2541 346 289
Fragments arranged by size:
2541 391 346 289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 979 3567
Size: 979 2588
AlwNI CAG_nnn'CTG
Cuts at: 0 168 231 2629 3567
Size: 168 63 2398 938
Fragments arranged by size:
2398 938 168 63
ApaLI G'TGCA_C
Cuts at: 0 1396 3567
Size: 1396 2171
ApoI r'AATT_y
Cuts at: 0 1998 2006 2561 3202 3567
Size: 1998 8 555 641 365
Fragments arranged by size:
1998 641 555 365 8
AvaI C'yCGr_G
Cuts at: 0 18 655 905 1198 1474 2812 3567
Size: 18 637 250 293 276 1338 755
Fragments arranged by size:
1338 755 637 293 276 250 18
BamHI G'GATC_C
Cuts at: 0 548 3567
Size: 548 3019
BanI G'GyrC_C
Cuts at: 0 33 245 1039 1170 1177 1774 1848 1926
Size: 33 212 794 131 7 597 74 78
Cuts at: 1926 3021 3567
Size: 1095 546
Fragments arranged by size:
1095 794 597 546 212 131 78 74
33 7
BanII G_rGCy'C
Cuts at: 0 296 527 607 656 2819 3350 3567
Size: 296 231 80 49 2163 531 217
Fragments arranged by size:
2163 531 296 231 217 80 49
BbeI G_GCGC'C
Cuts at: 0 249 1174 3567
Size: 249 925 2393
Fragments arranged by size:
2393 925 249
BbsI GAAGACnn'nnnn_
Cuts at: 0 941 1091 1345 1604 1703 2377 2487 2648
Size: 941 150 254 259 99 674 110 161
Cuts at: 2648 2837 3304 3323 3567
Size: 189 467 19 244
Fragments arranged by size:
941 674 467 259 254 244 189 161
150 110 99 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 1261 3230 3567
Size: 1261 1969 337
Fragments arranged by size:
1969 1261 337
BglI GCCn_nnn'nGGC
Cuts at: 0 1837 1846 3567
Size: 1837 9 1721
Fragments arranged by size:
1837 1721 9
BglII A'GATC_T
Cuts at: 0 1148 2326 3567
Size: 1148 1178 1241
Fragments arranged by size:
1241 1178 1148
BlpI GC'TnA_GC
Cuts at: 0 3041 3490 3567
Size: 3041 449 77
Fragments arranged by size:
3041 449 77
Bme1580I G_kGCm'C
Cuts at: 0 1400 1777 3026 3567
Size: 1400 377 1249 541
Fragments arranged by size:
1400 1249 541 377
BmgBI CAC'GTC
Cuts at: 0 299 3465 3567
Size: 299 3166 102
Fragments arranged by size:
3166 299 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 635 1343 3567
Size: 369 266 708 2224
Fragments arranged by size:
2224 708 369 266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 1427 2262 3567
Size: 345 1082 835 1305
Fragments arranged by size:
1305 1082 835 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 298 773 1046 1397 1640 2425 2780
Size: 163 135 475 273 351 243 785 355
Cuts at: 2780 3107 3567
Size: 327 460
Fragments arranged by size:
785 475 460 355 351 327 273 243
163 135
Bpu10I CC'TnA_GC
Cuts at: 0 728 1290 1370 3567
Size: 728 562 80 2197
Fragments arranged by size:
2197 728 562 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 3310 3567
Size: 1129 2181 257
Fragments arranged by size:
2181 1129 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 1014 1398 1420 3567
Size: 352 662 384 22 2147
Fragments arranged by size:
2147 662 384 352 22
BsaAI yAC'GTr
Cuts at: 0 1212 2306 2455 3567
Size: 1212 1094 149 1112
Fragments arranged by size:
1212 1112 1094 149
BsaBI GATnn'nnATC
Cuts at: 0 2807 3567
Size: 2807 760
BsaHI Gr'CG_yC
Cuts at: 0 246 317 632 1171 1962 2492 3567
Size: 246 71 315 539 791 530 1075
Fragments arranged by size:
1075 791 539 530 315 246 71
BsaWI w'CCGG_w
Cuts at: 0 2685 3567
Size: 2685 882
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1545 1575 1638 1668 3567
Size: 1545 30 63 30 1899
Fragments arranged by size:
1899 1545 63 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 273 595 760 1627 3400 3567
Size: 43 230 322 165 867 1773 167
Fragments arranged by size:
1773 867 322 230 167 165 43
BseYI C'CCAG_C
Cuts at: 0 168 685 2679 2847 3567
Size: 168 517 1994 168 720
Fragments arranged by size:
1994 720 517 168 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 991 1667 1967 2338 2654 3567
Size: 939 52 676 300 371 316 913
Fragments arranged by size:
939 913 676 371 316 300 52
BsiEI CG_ry'CG
Cuts at: 0 769 2895 3567
Size: 769 2126 672
Fragments arranged by size:
2126 769 672
BsiHKAI G_wGCw'C
Cuts at: 0 1371 1400 2476 3350 3567
Size: 1371 29 1076 874 217
Fragments arranged by size:
1371 1076 874 217 29
Bsp1286I G_dGCh'C
Cuts at: 0 296 527 607 656 1371 1400 1777 2476
Size: 296 231 80 49 715 29 377 699
Cuts at: 2476 2819 3026 3350 3567
Size: 343 207 324 217
Fragments arranged by size:
715 699 377 343 324 296 231 217
207 80 49 29
BspEI T'CCGG_A
Cuts at: 0 2685 3567
Size: 2685 882
BspHI T'CATG_A
Cuts at: 0 2078 3567
Size: 2078 1489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1366 1860 3567
Size: 1366 494 1707
Fragments arranged by size:
1707 1366 494
BsrBI CCG'CTC
Cuts at: 0 2864 2911 3567
Size: 2864 47 656
Fragments arranged by size:
2864 656 47
BsrDI GCAATG_nn'
Cuts at: 0 30 236 3567
Size: 30 206 3331
Fragments arranged by size:
3331 206 30
BsrFI r'CCGG_y
Cuts at: 0 1845 3548 3567
Size: 1845 1703 19
Fragments arranged by size:
1845 1703 19
BsrGI T'GTAC_A
Cuts at: 0 1048 3567
Size: 1048 2519
BssSI C'ACGA_G
Cuts at: 0 1696 3567
Size: 1696 1871
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1867 2924 3567
Size: 1867 1057 643
Fragments arranged by size:
1867 1057 643
BstBI TT'CG_AA
Cuts at: 0 87 3567
Size: 87 3480
BstEII G'GTnAC_C
Cuts at: 0 2871 3567
Size: 2871 696
BstYI r'GATC_y
Cuts at: 0 548 1148 2326 3567
Size: 548 600 1178 1241
Fragments arranged by size:
1241 1178 600 548
Bsu36I CC'TnA_GG
Cuts at: 0 38 1761 3567
Size: 38 1723 1806
Fragments arranged by size:
1806 1723 38
BtgI C'CryG_G
Cuts at: 0 249 1628 1664 1792 2933 3426 3567
Size: 249 1379 36 128 1141 493 141
Fragments arranged by size:
1379 1141 493 249 141 128 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 482 1973 2081 3567
Size: 482 1491 108 1486
Fragments arranged by size:
1491 1486 482 108
BtsI GCAGTG_nn'
Cuts at: 0 195 1451 3567
Size: 195 1256 2116
Fragments arranged by size:
2116 1256 195
DraIII CAC_nnn'GTG
Cuts at: 0 2973 3567
Size: 2973 594
EaeI y'GGCC_r
Cuts at: 0 2805 2892 3429 3567
Size: 2805 87 537 138
Fragments arranged by size:
2805 537 138 87
EagI C'GGCC_G
Cuts at: 0 2892 3567
Size: 2892 675
EarI CTCTTCn'nnn_
Cuts at: 0 417 1395 2224 2464 2548 3377 3567
Size: 417 978 829 240 84 829 190
Fragments arranged by size:
978 829 829 417 240 190 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2405 3567
Size: 2405 1162
EcoICRI GAG'CTC
Cuts at: 0 3348 3567
Size: 3348 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 298 475 773 1046 1397 1640 1808
Size: 163 135 177 298 273 351 243 168
Cuts at: 1808 2215 2290 2425 2481 2744 2780 3089 3107
Size: 407 75 135 56 263 36 309 18
Cuts at: 3107 3567
Size: 460
Fragments arranged by size:
460 407 351 309 298 273 263 243
177 168 163 135 135 75 56 36
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3250 3567
Size: 3250 317
EcoO109I rG'GnC_Cy
Cuts at: 0 536 586 2199 2898 3471 3501 3567
Size: 536 50 1613 699 573 30 66
Fragments arranged by size:
1613 699 573 536 66 50 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3021 3567
Size: 3021 546
HaeII r_GCGC'y
Cuts at: 0 249 353 576 1174 2482 3497 3549 3567
Size: 249 104 223 598 1308 1015 52 18
Fragments arranged by size:
1308 1015 598 249 223 104 52 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 436 468 540 572 1060 1092
Size: 283 32 121 32 72 32 488 32
Cuts at: 1092 1545 1577 1591 1623 2157 2189 2635 2667
Size: 453 32 14 32 534 32 446 32
Cuts at: 2667 3567
Size: 900
Fragments arranged by size:
900 534 488 453 446 283 121 72
32 32 32 32 32 32 32 32
14
KasI G'GCGC_C
Cuts at: 0 245 1170 3567
Size: 245 925 2397
Fragments arranged by size:
2397 925 245
KpnI G_GTAC'C
Cuts at: 0 1930 3567
Size: 1930 1637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 2405 3567
Size: 692 1713 1162
Fragments arranged by size:
1713 1162 692
MscI TGG'CCA
Cuts at: 0 2807 3567
Size: 2807 760
MslI CAynn'nnrTG
Cuts at: 0 391 2765 2932 2968 3278 3567
Size: 391 2374 167 36 310 289
Fragments arranged by size:
2374 391 310 289 167 36
MspA1I CmG'CkG
Cuts at: 0 168 251 391 860 1666 1828 1858 1894
Size: 168 83 140 469 806 162 30 36
Cuts at: 1894 2695 2826 3489 3567
Size: 801 131 663 78
Fragments arranged by size:
806 801 663 469 168 162 140 131
83 78 36 30
NaeI GCC'GGC
Cuts at: 0 3550 3567
Size: 3550 17
NarI GG'CG_CC
Cuts at: 0 246 1171 3567
Size: 246 925 2396
Fragments arranged by size:
2396 925 246
NcoI C'CATG_G
Cuts at: 0 1628 1792 3567
Size: 1628 164 1775
Fragments arranged by size:
1775 1628 164
NgoMIV G'CCGG_C
Cuts at: 0 3548 3567
Size: 3548 19
NspI r_CATG'y
Cuts at: 0 267 2508 3189 3567
Size: 267 2241 681 378
Fragments arranged by size:
2241 681 378 267
PasI CC'CwG_GG
Cuts at: 0 2788 3567
Size: 2788 779
PflMI CCAn_nnn'nTGG
Cuts at: 0 1816 2803 2973 3567
Size: 1816 987 170 594
Fragments arranged by size:
1816 987 594 170
PfoI T'CCnGG_A
Cuts at: 0 2796 3567
Size: 2796 771
PmlI CAC'GTG
Cuts at: 0 1212 2306 2455 3567
Size: 1212 1094 149 1112
Fragments arranged by size:
1212 1112 1094 149
PpuMI rG'GwC_Cy
Cuts at: 0 586 2199 2898 3471 3501 3567
Size: 586 1613 699 573 30 66
Fragments arranged by size:
1613 699 586 573 66 30
PspXI vC'TCGA_Gb
Cuts at: 0 905 3567
Size: 905 2662
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1919 1951 3567
Size: 1919 32 1616
Fragments arranged by size:
1919 1616 32
PstI C_TGCA'G
Cuts at: 0 1240 1857 3567
Size: 1240 617 1710
Fragments arranged by size:
1710 1240 617
PvuII CAG'CTG
Cuts at: 0 168 391 1828 2695 3567
Size: 168 223 1437 867 872
Fragments arranged by size:
1437 872 867 223 168
RsrII CG'GwC_CG
Cuts at: 0 1843 3567
Size: 1843 1724
SacI G_AGCT'C
Cuts at: 0 3350 3567
Size: 3350 217
SacII CC_GC'GG
Cuts at: 0 252 1667 3567
Size: 252 1415 1900
Fragments arranged by size:
1900 1415 252
SanDI GG'GwC_CC
Cuts at: 0 586 2898 3567
Size: 586 2312 669
Fragments arranged by size:
2312 669 586
SapI GCTCTTCn'nnn_
Cuts at: 0 2464 3567
Size: 2464 1103
SexAI A'CCwGG_T
Cuts at: 0 3537 3567
Size: 3537 30
SfcI C'TryA_G
Cuts at: 0 1236 1853 3567
Size: 1236 617 1714
Fragments arranged by size:
1714 1236 617
SfoI GGC'GCC
Cuts at: 0 247 1172 3567
Size: 247 925 2395
Fragments arranged by size:
2395 925 247
SmaI CCC'GGG
Cuts at: 0 1200 3567
Size: 1200 2367
SmlI C'TyrA_G
Cuts at: 0 905 1144 3325 3567
Size: 905 239 2181 242
Fragments arranged by size:
2181 905 242 239
SphI G_CATG'C
Cuts at: 0 267 3567
Size: 267 3300
StuI AGG'CCT
Cuts at: 0 2744 3567
Size: 2744 823
StyI C'CwwG_G
Cuts at: 0 1089 1628 1792 1965 3567
Size: 1089 539 164 173 1602
Fragments arranged by size:
1602 1089 539 173 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 783 2980 3221 3548 3567
Size: 783 2197 241 327 19
Fragments arranged by size:
2197 783 327 241 19
TatI w'GTAC_w
Cuts at: 0 1048 1232 3567
Size: 1048 184 2335
Fragments arranged by size:
2335 1048 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2444 2476 3567
Size: 2444 32 1091
Fragments arranged by size:
2444 1091 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 2400 3567
Size: 621 1779 1167
Fragments arranged by size:
1779 1167 621
XhoI C'TCGA_G
Cuts at: 0 905 3567
Size: 905 2662
XmaI C'CCGG_G
Cuts at: 0 1198 3567
Size: 1198 2369
XmnI GAAnn'nnTTC
Cuts at: 0 872 3567
Size: 872 2695
ZraI GAC'GTC
Cuts at: 0 2493 3567
Size: 2493 1074
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AhdI AleI AlfI
AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PspXI PsrI PstI PvuII
RsrII SacI SacII SanDI SapI SexAI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AloI ApaI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI
BmtI BsiWI BsmI BsmBI BssHII BstXI BstZ17I ClaI
CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PvuI SalI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI