Restriction map for
pF1KSDA0334
(Linear) (Six-base) MAPSORT of: KIAA0334.seq Check: 1069 from: 1 to: 2538
KIAA0334.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
Acc65I G'GTAC_C
Cuts at: 0 2172 2538
Size: 2172 366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1036 1648 2288 2538
Size: 665 371 612 640 250
Fragments arranged by size:
665 640 612 371 250
AleI CACnn'nnGTG
Cuts at: 0 1993 2538
Size: 1993 545
AlwNI CAG_nnn'CTG
Cuts at: 0 1198 1686 2538
Size: 1198 488 852
Fragments arranged by size:
1198 852 488
ApaLI G'TGCA_C
Cuts at: 0 798 2538
Size: 798 1740
ApoI r'AATT_y
Cuts at: 0 284 574 640 651 957 1086 1455 1509
Size: 284 290 66 11 306 129 369 54
Cuts at: 1509 1649 1722 1752 2538
Size: 140 73 30 786
Fragments arranged by size:
786 369 306 290 284 140 129 73
66 54 30 11
AseI AT'TA_AT
Cuts at: 0 337 2538
Size: 337 2201
BamHI G'GATC_C
Cuts at: 0 172 2538
Size: 172 2366
BanI G'GyrC_C
Cuts at: 0 876 2172 2538
Size: 876 1296 366
Fragments arranged by size:
1296 876 366
BanII G_rGCy'C
Cuts at: 0 34 2284 2538
Size: 34 2250 254
Fragments arranged by size:
2250 254 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 684 1365 2538
Size: 684 681 1173
Fragments arranged by size:
1173 684 681
BclI T'GATC_A
Cuts at: 0 1272 2538
Size: 1272 1266
BlpI GC'TnA_GC
Cuts at: 0 2327 2538
Size: 2327 211
Bme1580I G_kGCm'C
Cuts at: 0 802 879 1928 2538
Size: 802 77 1049 610
Fragments arranged by size:
1049 802 610 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 178 1455 1530 1943 2538
Size: 178 1277 75 413 595
Fragments arranged by size:
1277 595 413 178 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1484 2096 2538
Size: 1234 250 612 442
Fragments arranged by size:
1234 612 442 250
BsaBI GATnn'nnATC
Cuts at: 0 935 2538
Size: 935 1603
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1365 1395 2538
Size: 1365 30 1143
Fragments arranged by size:
1365 1143 30
BseYI C'CCAG_C
Cuts at: 0 2336 2538
Size: 2336 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 2538
Size: 248 2290
BsiEI CG_ry'CG
Cuts at: 0 1331 2538
Size: 1331 1207
BsiHKAI G_wGCw'C
Cuts at: 0 34 802 980 1381 1966 2284 2434 2538
Size: 34 768 178 401 585 318 150 104
Fragments arranged by size:
768 585 401 318 178 150 104 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1338 2538
Size: 1338 1200
Bsp1286I G_dGCh'C
Cuts at: 0 34 802 879 980 1381 1928 1966 2284
Size: 34 768 77 101 401 547 38 318
Cuts at: 2284 2434 2538
Size: 150 104
Fragments arranged by size:
768 547 401 318 150 104 101 77
38 34
BspHI T'CATG_A
Cuts at: 0 374 972 1407 2538
Size: 374 598 435 1131
Fragments arranged by size:
1131 598 435 374
BsrFI r'CCGG_y
Cuts at: 0 2489 2538
Size: 2489 49
BstAPI GCAn_nnn'nTGC
Cuts at: 0 984 2538
Size: 984 1554
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1216 1468 2538
Size: 1216 252 1070
Fragments arranged by size:
1216 1070 252
BstYI r'GATC_y
Cuts at: 0 172 1361 2538
Size: 172 1189 1177
Fragments arranged by size:
1189 1177 172
BtgI C'CryG_G
Cuts at: 0 1674 2376 2538
Size: 1674 702 162
Fragments arranged by size:
1674 702 162
BtsI GCAGTG_nn'
Cuts at: 0 260 2538
Size: 260 2278
DraI TTT'AAA
Cuts at: 0 554 665 2538
Size: 554 111 1873
Fragments arranged by size:
1873 554 111
EaeI y'GGCC_r
Cuts at: 0 1328 2538
Size: 1328 1210
EagI C'GGCC_G
Cuts at: 0 1328 2538
Size: 1328 1210
EarI CTCTTCn'nnn_
Cuts at: 0 317 684 815 1169 1187 1302 2538
Size: 317 367 131 354 18 115 1236
Fragments arranged by size:
1236 367 354 317 131 115 18
EcoICRI GAG'CTC
Cuts at: 0 32 2282 2538
Size: 32 2250 256
Fragments arranged by size:
2250 256 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1036 1648 2288 2538
Size: 665 371 612 640 250
Fragments arranged by size:
665 640 612 371 250
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1027 2538
Size: 1027 1511
EcoRI G'AATT_C
Cuts at: 0 574 1086 2538
Size: 574 512 1452
Fragments arranged by size:
1452 574 512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1198 1678 2538
Size: 1198 480 860
Fragments arranged by size:
1198 860 480
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 1204 1236 1330 1362 2196 2228
Size: 814 32 358 32 94 32 834 32
Cuts at: 2228 2538
Size: 310
Fragments arranged by size:
834 814 358 310 94 32 32 32
32
HincII GTy'rAC
Cuts at: 0 42 1906 2119 2538
Size: 42 1864 213 419
Fragments arranged by size:
1864 419 213 42
KpnI G_GTAC'C
Cuts at: 0 2176 2538
Size: 2176 362
MfeI C'AATT_G
Cuts at: 0 1585 2538
Size: 1585 953
MslI CAynn'nnrTG
Cuts at: 0 944 1993 2538
Size: 944 1049 545
Fragments arranged by size:
1049 944 545
MspA1I CmG'CkG
Cuts at: 0 1195 2331 2538
Size: 1195 1136 207
Fragments arranged by size:
1195 1136 207
NaeI GCC'GGC
Cuts at: 0 2491 2538
Size: 2491 47
NcoI C'CATG_G
Cuts at: 0 1674 2376 2538
Size: 1674 702 162
Fragments arranged by size:
1674 702 162
NgoMIV G'CCGG_C
Cuts at: 0 2489 2538
Size: 2489 49
NspI r_CATG'y
Cuts at: 0 591 1533 2538
Size: 591 942 1005
Fragments arranged by size:
1005 942 591
PsiI TTA'TAA
Cuts at: 0 500 2538
Size: 500 2038
PstI C_TGCA'G
Cuts at: 0 224 1691 2013 2073 2538
Size: 224 1467 322 60 465
Fragments arranged by size:
1467 465 322 224 60
PvuII CAG'CTG
Cuts at: 0 1195 2331 2538
Size: 1195 1136 207
Fragments arranged by size:
1195 1136 207
SacI G_AGCT'C
Cuts at: 0 34 2284 2538
Size: 34 2250 254
Fragments arranged by size:
2250 254 34
ScaI AGT'ACT
Cuts at: 0 2181 2538
Size: 2181 357
SfcI C'TryA_G
Cuts at: 0 18 220 1121 1687 2009 2069 2150 2538
Size: 18 202 901 566 322 60 81 388
Fragments arranged by size:
901 566 388 322 202 81 60 18
SmlI C'TyrA_G
Cuts at: 0 1249 1499 2111 2538
Size: 1249 250 612 427
Fragments arranged by size:
1249 612 427 250
SspI AAT'ATT
Cuts at: 0 550 1620 2538
Size: 550 1070 918
Fragments arranged by size:
1070 918 550
StyI C'CwwG_G
Cuts at: 0 1674 2376 2522 2538
Size: 1674 702 146 16
Fragments arranged by size:
1674 702 146 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 897 1401 2074 2538
Size: 897 504 673 464
Fragments arranged by size:
897 673 504 464
TatI w'GTAC_w
Cuts at: 0 1111 1888 1914 2179 2538
Size: 1111 777 26 265 359
Fragments arranged by size:
1111 777 359 265 26
Tth111I GACn'n_nGTC
Cuts at: 0 2250 2538
Size: 2250 288
XbaI T'CTAG_A
Cuts at: 0 112 832 864 2538
Size: 112 720 32 1674
Fragments arranged by size:
1674 720 112 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1822 2538
Size: 1822 716
XmnI GAAnn'nnTTC
Cuts at: 0 1661 2538
Size: 1661 877
Enzymes that do cut:
Acc65I AcuI AleI AlwNI ApaLI ApoI AseI BamHI
BanI BanII BciVI BclI BlpI Bme1580I BmrI BpuEI
BsaBI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BsrFI BstAPI BstXI BstYI BtgI BtsI DraI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoRI FalI
Hin4I HincII KpnI MfeI MslI MspA1I NaeI NcoI
NgoMIV NspI PsiI PstI PvuII SacI ScaI SfcI
SmlI SspI StyI TaqII TatI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BbeI BbsI BbvCI BcgI BfrBI BglI BglII
BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BseRI BsiWI BsmI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EciI EcoO109I EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI MluI MmeI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
XhoI XmaI ZraI