Restriction map for
pF1KA0338
(Linear) (Six-base) MAPSORT of: KIAA0338.seq Check: 2252 from: 1 to: 2643
KIAA0338.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AccI GT'mk_AC
Cuts at: 0 854 2588 2643
Size: 854 1734 55
Fragments arranged by size:
1734 854 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 1573 1801 2643
Size: 1534 39 228 842
Fragments arranged by size:
1534 842 228 39
AgeI A'CCGG_T
Cuts at: 0 1070 2643
Size: 1070 1573
AleI CACnn'nnGTG
Cuts at: 0 91 2643
Size: 91 2552
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1759 1791 2643
Size: 1759 32 852
Fragments arranged by size:
1759 852 32
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1348 1402 2523 2643
Size: 363 985 54 1121 120
Fragments arranged by size:
1121 985 363 120 54
ApaI G_GGCC'C
Cuts at: 0 1725 1995 2643
Size: 1725 270 648
Fragments arranged by size:
1725 648 270
ApoI r'AATT_y
Cuts at: 0 501 2643
Size: 501 2142
AvaI C'yCGr_G
Cuts at: 0 64 740 795 1718 1973 2643
Size: 64 676 55 923 255 670
Fragments arranged by size:
923 676 670 255 64 55
BanI G'GyrC_C
Cuts at: 0 1645 2389 2643
Size: 1645 744 254
Fragments arranged by size:
1645 744 254
BanII G_rGCy'C
Cuts at: 0 649 719 1142 1171 1541 1562 1690 1725
Size: 649 70 423 29 370 21 128 35
Cuts at: 1725 1928 1995 2081 2643
Size: 203 67 86 562
Fragments arranged by size:
649 562 423 370 203 128 86 70
67 35 29 21
BbeI G_GCGC'C
Cuts at: 0 2393 2643
Size: 2393 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 560 1907 2643
Size: 560 1347 736
Fragments arranged by size:
1347 736 560
BbvCI CC'TCA_GC
Cuts at: 0 686 1403 1678 1853 2643
Size: 686 717 275 175 790
Fragments arranged by size:
790 717 686 275 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 55 89 1878 1912 2030 2064 2643
Size: 55 34 1789 34 118 34 579
Fragments arranged by size:
1789 579 118 55 34 34 34
BglII A'GATC_T
Cuts at: 0 977 2643
Size: 977 1666
BlpI GC'TnA_GC
Cuts at: 0 665 2643
Size: 665 1978
Bme1580I G_kGCm'C
Cuts at: 0 1648 1725 1995 2643
Size: 1648 77 270 648
Fragments arranged by size:
1648 648 270 77
BmgBI CAC'GTC
Cuts at: 0 1714 2643
Size: 1714 929
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 1602 2064 2501 2643
Size: 652 950 462 437 142
Fragments arranged by size:
950 652 462 437 142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 144 2643
Size: 144 2499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1800 1867 2643
Size: 769 1031 67 776
Fragments arranged by size:
1031 776 769 67
Bpu10I CC'TnA_GC
Cuts at: 0 41 686 1136 1403 1678 1853 1922 2643
Size: 41 645 450 267 275 175 69 721
Fragments arranged by size:
721 645 450 275 267 175 69 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 2263 2643
Size: 958 1305 380
Fragments arranged by size:
1305 958 380
BsaI GGTCTCn'nnnn_
Cuts at: 0 2242 2643
Size: 2242 401
BsaBI GATnn'nnATC
Cuts at: 0 2507 2643
Size: 2507 136
BsaHI Gr'CG_yC
Cuts at: 0 2390 2643
Size: 2390 253
BsaWI w'CCGG_w
Cuts at: 0 485 1070 1178 2643
Size: 485 585 108 1465
Fragments arranged by size:
1465 585 485 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 2643
Size: 313 30 2300
Fragments arranged by size:
2300 313 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 205 703 766 1005 1420 1608 1708
Size: 61 144 498 63 239 415 188 100
Cuts at: 1708 2629 2643
Size: 921 14
Fragments arranged by size:
921 498 415 239 188 144 100 63
61 14
BseYI C'CCAG_C
Cuts at: 0 149 238 542 669 1314 1996 2180 2643
Size: 149 89 304 127 645 682 184 463
Fragments arranged by size:
682 645 463 304 184 149 127 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 844 1223 2643
Size: 679 165 379 1420
Fragments arranged by size:
1420 679 379 165
BsiEI CG_ry'CG
Cuts at: 0 1932 2643
Size: 1932 711
BsiHKAI G_wGCw'C
Cuts at: 0 719 1045 1541 1816 1928 2643
Size: 719 326 496 275 112 715
Fragments arranged by size:
719 715 496 326 275 112
BsmI GAATG_Cn'
Cuts at: 0 831 2643
Size: 831 1812
Bsp1286I G_dGCh'C
Cuts at: 0 649 719 1045 1142 1171 1541 1562 1648
Size: 649 70 326 97 29 370 21 86
Cuts at: 1648 1690 1725 1816 1928 1995 2081 2643
Size: 42 35 91 112 67 86 562
Fragments arranged by size:
649 562 370 326 112 97 91 86
86 70 67 42 35 29 21
BspEI T'CCGG_A
Cuts at: 0 485 2643
Size: 485 2158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 866 2175 2643
Size: 866 1309 468
Fragments arranged by size:
1309 866 468
BsrFI r'CCGG_y
Cuts at: 0 608 935 1070 1232 2643
Size: 608 327 135 162 1411
Fragments arranged by size:
1411 608 327 162 135
BstBI TT'CG_AA
Cuts at: 0 1898 2643
Size: 1898 745
BstEII G'GTnAC_C
Cuts at: 0 2571 2643
Size: 2571 72
BstYI r'GATC_y
Cuts at: 0 482 968 977 1010 1977 2643
Size: 482 486 9 33 967 666
Fragments arranged by size:
967 666 486 482 33 9
BstZ17I GTA'TAC
Cuts at: 0 2589 2643
Size: 2589 54
BtgI C'CryG_G
Cuts at: 0 108 1884 2243 2264 2330 2643
Size: 108 1776 359 21 66 313
Fragments arranged by size:
1776 359 313 108 66 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1757 2643
Size: 1757 886
BtsI GCAGTG_nn'
Cuts at: 0 2393 2643
Size: 2393 250
DrdI GACnn_nn'nnGTC
Cuts at: 0 1867 2643
Size: 1867 776
EaeI y'GGCC_r
Cuts at: 0 111 348 1796 2114 2327 2531 2643
Size: 111 237 1448 318 213 204 112
Fragments arranged by size:
1448 318 237 213 204 112 111
EcoICRI GAG'CTC
Cuts at: 0 717 1539 1926 2643
Size: 717 822 387 717
Fragments arranged by size:
822 717 717 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1534 1573 1800 1801 1867 2643
Size: 769 765 39 227 1 66 776
Fragments arranged by size:
776 769 765 227 66 39 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 987 2643
Size: 400 587 1656
Fragments arranged by size:
1656 587 400
EcoO109I rG'GnC_Cy
Cuts at: 0 16 51 1193 1345 1547 1722 1959 1991
Size: 16 35 1142 152 202 175 237 32
Cuts at: 1991 1992 2045 2520 2643
Size: 1 53 475 123
Fragments arranged by size:
1142 475 237 202 175 152 123 53
35 32 16 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 426 2643
Size: 426 2217
HaeII r_GCGC'y
Cuts at: 0 1223 1895 2393 2643
Size: 1223 672 498 250
Fragments arranged by size:
1223 672 498 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1363 1395 1996 2028 2239 2271 2643
Size: 1363 32 601 32 211 32 372
Fragments arranged by size:
1363 601 372 211 32 32 32
HindIII A'AGCT_T
Cuts at: 0 835 2643
Size: 835 1808
KasI G'GCGC_C
Cuts at: 0 2389 2643
Size: 2389 254
MfeI C'AATT_G
Cuts at: 0 1046 2643
Size: 1046 1597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1450 2643
Size: 1450 1193
MscI TGG'CCA
Cuts at: 0 1798 2116 2329 2533 2643
Size: 1798 318 213 204 110
Fragments arranged by size:
1798 318 213 204 110
MslI CAynn'nnrTG
Cuts at: 0 91 2643
Size: 91 2552
MspA1I CmG'CkG
Cuts at: 0 79 546 585 2299 2643
Size: 79 467 39 1714 344
Fragments arranged by size:
1714 467 344 79 39
NaeI GCC'GGC
Cuts at: 0 610 2643
Size: 610 2033
NarI GG'CG_CC
Cuts at: 0 2390 2643
Size: 2390 253
NcoI C'CATG_G
Cuts at: 0 1884 2264 2643
Size: 1884 380 379
Fragments arranged by size:
1884 380 379
NgoMIV G'CCGG_C
Cuts at: 0 608 2643
Size: 608 2035
NspI r_CATG'y
Cuts at: 0 637 697 2643
Size: 637 60 1946
Fragments arranged by size:
1946 637 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 2523 2643
Size: 2523 120
PfoI T'CCnGG_A
Cuts at: 0 2284 2643
Size: 2284 359
PpuMI rG'GwC_Cy
Cuts at: 0 1193 1547 2643
Size: 1193 354 1096
Fragments arranged by size:
1193 1096 354
PshAI GACnn'nnGTC
Cuts at: 0 2158 2643
Size: 2158 485
PspOMI G'GGCC_C
Cuts at: 0 1721 1991 2643
Size: 1721 270 652
Fragments arranged by size:
1721 652 270
PstI C_TGCA'G
Cuts at: 0 105 584 2153 2555 2643
Size: 105 479 1569 402 88
Fragments arranged by size:
1569 479 402 105 88
PvuII CAG'CTG
Cuts at: 0 546 585 2299 2643
Size: 546 39 1714 344
Fragments arranged by size:
1714 546 344 39
SacI G_AGCT'C
Cuts at: 0 719 1541 1928 2643
Size: 719 822 387 715
Fragments arranged by size:
822 719 715 387
SbfI CC_TGCA'GG
Cuts at: 0 105 2643
Size: 105 2538
SexAI A'CCwGG_T
Cuts at: 0 371 2643
Size: 371 2272
SfcI C'TryA_G
Cuts at: 0 101 201 580 2149 2551 2643
Size: 101 100 379 1569 402 92
Fragments arranged by size:
1569 402 379 101 100 92
SfoI GGC'GCC
Cuts at: 0 2391 2643
Size: 2391 252
SmaI CCC'GGG
Cuts at: 0 742 797 1720 1975 2643
Size: 742 55 923 255 668
Fragments arranged by size:
923 742 668 255 55
SmlI C'TyrA_G
Cuts at: 0 973 2278 2643
Size: 973 1305 365
Fragments arranged by size:
1305 973 365
SphI G_CATG'C
Cuts at: 0 637 2643
Size: 637 2006
StyI C'CwwG_G
Cuts at: 0 464 869 1148 1640 1884 2264 2643
Size: 464 405 279 492 244 380 379
Fragments arranged by size:
492 464 405 380 379 279 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 131 661 1161 1311 2194 2440 2643
Size: 131 530 500 150 883 246 203
Fragments arranged by size:
883 530 500 246 203 150 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 2643
Size: 313 32 2298
Fragments arranged by size:
2298 313 32
Tth111I GACn'n_nGTC
Cuts at: 0 373 2643
Size: 373 2270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1155 2188 2643
Size: 1155 1033 455
Fragments arranged by size:
1155 1033 455
XmaI C'CCGG_G
Cuts at: 0 740 795 1718 1973 2643
Size: 740 55 923 255 670
Fragments arranged by size:
923 740 670 255 55
Enzymes that do cut:
AccI AcuI AgeI AleI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI DrdI EaeI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PfoI PpuMI PshAI PspOMI
PstI PvuII SacI SbfI SexAI SfcI SfoI SmaI
SmlI SphI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglI BmtI BsaAI BsiWI BsmBI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstXI
Bsu36I ClaI CspCI DraI DraIII EagI EarI EciI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI ScaI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI
XbaI XhoI XmnI ZraI